(Circular) (Six-base) MAPSORT of: pFN21AE3408.seq Check: 4147 from: 1 to: 5584 pFN21AE3408 5584 bp With 182 enzymes: SgfI * March 28, 2012 12:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3394 278 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2927 1937 Size: 990 4594 Acc65I G'GTAC_C Cuts at: 2905 2905 Size: 5584 AclI AA'CG_TT Cuts at: 1563 3711 4084 1563 Size: 2148 373 3063 Fragments arranged by size: 3063 2148 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2769 3647 5031 5299 1632 Size: 1137 878 1384 268 1917 Fragments arranged by size: 1917 1384 1137 878 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5387 AflIII A'CryG_T Cuts at: 1203 4483 5460 1203 Size: 3280 977 1327 Fragments arranged by size: 3280 1327 977 AgeI A'CCGG_T Cuts at: 4415 4415 Size: 5584 AhdI GACnn_n'nnGTC Cuts at: 4313 4313 Size: 5584 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5584 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5584 AlwNI CAG_nnn'CTG Cuts at: 2065 3327 4899 2065 Size: 1262 1572 2750 Fragments arranged by size: 2750 1572 1262 ApaLI G'TGCA_C Cuts at: 2685 3641 4797 2685 Size: 956 1156 3472 Fragments arranged by size: 3472 1156 956 ApoI r'AATT_y Cuts at: 1493 2236 2375 2383 2487 2503 2571 2893 3119 Size: 743 139 8 104 16 68 322 226 Cuts at: 3119 3271 5380 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 743 322 226 152 139 104 68 16 8 AseI AT'TA_AT Cuts at: 160 1032 4138 160 Size: 872 3106 1606 Fragments arranged by size: 3106 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 AvaI C'yCGr_G Cuts at: 1870 1943 2909 1870 Size: 73 966 4545 Fragments arranged by size: 4545 966 73 BamHI G'GATC_C Cuts at: 2914 3281 2914 Size: 367 5217 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2905 4360 618 Size: 325 217 627 1118 1455 1842 Fragments arranged by size: 1842 1455 1118 627 325 217 BanII G_rGCy'C Cuts at: 1391 2903 1391 Size: 1512 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4613 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3775 3809 1903 Size: 34 1838 34 3678 Fragments arranged by size: 3678 1838 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3476 4686 3476 Size: 1210 4374 BclI T'GATC_A Cuts at: 1548 1548 Size: 5584 BfrBI ATG'CAT Cuts at: 2371 2371 Size: 5584 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4195 136 Size: 107 122 71 631 605 2523 1525 Fragments arranged by size: 2523 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5579 5579 Size: 5584 BlpI GC'TnA_GC Cuts at: 3001 3001 Size: 5584 Bme1580I G_kGCm'C Cuts at: 1790 2689 3645 4801 1790 Size: 899 956 1156 2573 Fragments arranged by size: 2573 1156 956 899 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5584 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3315 4273 442 Size: 416 53 83 399 24 1898 958 1753 Fragments arranged by size: 1898 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5584 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2601 2685 2853 4244 1239 Size: 1362 84 168 1391 2579 Fragments arranged by size: 2579 1391 1362 168 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3709 4574 4872 3709 Size: 865 298 4421 Fragments arranged by size: 4421 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4247 882 Size: 617 2748 2219 Fragments arranged by size: 2748 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3375 493 Size: 711 2171 2702 Fragments arranged by size: 2702 2171 711 BsaBI GATnn'nnATC Cuts at: 5578 5578 Size: 5584 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3391 3773 4408 275 Size: 53 83 186 936 1858 382 635 1451 Fragments arranged by size: 1858 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4017 4415 4689 4836 5247 4017 Size: 398 274 147 411 4354 Fragments arranged by size: 4354 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2045 2075 1178 Size: 30 165 30 642 30 4687 Fragments arranged by size: 4687 642 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 4704 BseYI C'CCAG_C Cuts at: 4787 5283 4787 Size: 496 5088 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2058 1163 Size: 573 322 4689 Fragments arranged by size: 4689 573 322 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3034 3795 4823 1540 Size: 275 186 1033 761 1028 2301 Fragments arranged by size: 2301 1033 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2689 3645 3730 4801 2689 Size: 956 85 1071 3472 Fragments arranged by size: 3472 1071 956 85 BsmI GAATG_Cn' Cuts at: 3095 3188 3095 Size: 93 5491 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2689 2903 3645 3730 4801 1391 Size: 399 899 214 742 85 1071 2174 Fragments arranged by size: 2174 1071 899 742 399 214 85 BspEI T'CCGG_A Cuts at: 5247 5247 Size: 5584 BspHI T'CATG_A Cuts at: 3473 5203 3473 Size: 1730 3854 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2939 844 Size: 2095 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3471 1394 Size: 429 1648 3507 Fragments arranged by size: 3507 1648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4079 4253 65 Size: 1183 2831 174 1396 Fragments arranged by size: 2831 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4228 4415 1700 Size: 110 2418 187 2869 Fragments arranged by size: 2869 2418 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5584 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5584 BssSI C'ACGA_G Cuts at: 3644 4656 3644 Size: 1012 4572 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5584 BstBI TT'CG_AA Cuts at: 4388 4388 Size: 5584 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5584 BstYI r'GATC_y Cuts at: 1972 2914 3281 3666 3683 5135 5221 5233 5579 Size: 942 367 385 17 1452 86 12 346 Cuts at: 5579 1972 Size: 1977 Fragments arranged by size: 1977 1452 942 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4093 Fragments arranged by size: 4093 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4845 Fragments arranged by size: 4845 683 56 BtsI GCAGTG_nn' Cuts at: 2066 2070 3100 3894 3914 2066 Size: 4 1030 794 20 3736 Fragments arranged by size: 3736 1030 794 20 4 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5584 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5549 DraI TTT'AAA Cuts at: 2707 2888 2707 Size: 181 5403 DraIII CAC_nnn'GTG Cuts at: 2693 2693 Size: 5584 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4591 809 Size: 507 3275 1802 Fragments arranged by size: 3275 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 3031 3920 Size: 54 1425 50 275 18 201 1000 889 Cuts at: 3920 5549 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1000 889 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3031 1537 Size: 275 1219 4090 Fragments arranged by size: 4090 1219 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2514 2745 3514 1353 Size: 1161 231 769 3423 Fragments arranged by size: 3423 1161 769 231 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4171 4545 4691 387 Size: 1064 138 2582 374 146 1280 Fragments arranged by size: 2582 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2601 2685 2769 2853 3647 4244 5031 Size: 393 969 84 84 84 794 597 787 Cuts at: 5031 5299 1239 Size: 268 1524 Fragments arranged by size: 1524 969 794 787 597 393 268 84 84 84 EcoRI G'AATT_C Cuts at: 2893 3271 2893 Size: 378 5206 FspI TGC'GCA Cuts at: 4090 4090 Size: 5584 HaeII r_GCGC'y Cuts at: 1678 4731 1678 Size: 3053 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4225 4257 4299 4331 1373 Size: 32 135 32 2653 32 42 32 2626 Fragments arranged by size: 2653 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2928 3174 669 Size: 988 281 990 246 3079 Fragments arranged by size: 3079 990 988 281 246 HindIII A'AGCT_T Cuts at: 748 2868 748 Size: 2120 3464 HpaI GTT'AAC Cuts at: 3174 3174 Size: 5584 KpnI G_GTAC'C Cuts at: 2909 2909 Size: 5584 MfeI C'AATT_G Cuts at: 3183 3183 Size: 5584 MluI A'CGCG_T Cuts at: 5460 5460 Size: 5584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4698 4882 1305 Size: 3393 184 2007 Fragments arranged by size: 3393 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2033 10 Size: 54 1425 343 201 3561 Fragments arranged by size: 3561 1425 343 201 54 MslI CAynn'nnrTG Cuts at: 518 1166 2848 3542 3901 4060 518 Size: 648 1682 694 359 159 2042 Fragments arranged by size: 2042 1682 694 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 3000 3677 4825 5070 1606 Size: 474 920 677 1148 245 2120 Fragments arranged by size: 2120 1148 920 677 474 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4093 Fragments arranged by size: 4093 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5584 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5584 NotI GC'GGCC_GC Cuts at: 3031 3031 Size: 5584 NsiI A_TGCA'T Cuts at: 2373 2373 Size: 5584 NspI r_CATG'y Cuts at: 1132 1229 2099 2461 2942 4487 1132 Size: 97 870 362 481 1545 2229 Fragments arranged by size: 2229 1545 870 481 362 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5584 PciI A'CATG_T Cuts at: 4483 4483 Size: 5584 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5584 PmeI GTTT'AAAC Cuts at: 2888 2888 Size: 5584 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3653 3685 5166 5198 3653 Size: 32 1481 32 4039 Fragments arranged by size: 4039 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5584 PsiI TTA'TAA Cuts at: 2298 3154 2298 Size: 856 4728 PstI C_TGCA'G Cuts at: 830 2936 830 Size: 2106 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5584 SalI G'TCGA_C Cuts at: 1936 2926 1936 Size: 990 4594 SbfI CC_TGCA'GG Cuts at: 2936 2936 Size: 5584 ScaI AGT'ACT Cuts at: 3832 5544 3832 Size: 1712 3872 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 5584 SfcI C'TryA_G Cuts at: 826 1045 2113 2932 4067 4748 4939 826 Size: 219 1068 819 1135 681 191 1471 Fragments arranged by size: 1471 1135 1068 819 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5584 SmaI CCC'GGG Cuts at: 1872 2911 1872 Size: 1039 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3688 4589 4851 820 Size: 197 926 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3375 493 Size: 2882 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5584 SphI G_CATG'C Cuts at: 1132 2461 2942 1132 Size: 1329 481 3774 Fragments arranged by size: 3774 1329 481 SspI AAT'ATT Cuts at: 5 52 2340 3508 5 Size: 47 2288 1168 2081 Fragments arranged by size: 2288 2081 1168 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5584 StyI C'CwwG_G Cuts at: 513 1065 2004 2287 3023 513 Size: 552 939 283 736 3074 Fragments arranged by size: 3074 939 736 552 283 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3611 3628 3781 3966 4411 325 Size: 1255 2031 17 153 185 445 1498 Fragments arranged by size: 2031 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3830 5542 96 Size: 274 80 33 51 1443 1853 1712 138 Fragments arranged by size: 1853 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2045 2077 5544 5576 2045 Size: 32 3467 32 2053 Fragments arranged by size: 3467 2053 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5584 XbaI T'CTAG_A Cuts at: 2920 2920 Size: 5584 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5584 XmaI C'CCGG_G Cuts at: 1870 2909 1870 Size: 1039 4545 XmnI GAAnn'nnTTC Cuts at: 3713 3713 Size: 5584 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3392 276 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI