(Circular) (Six-base) MAPSORT of: pFN21AE0525.seq Check: 6498 from: 1 to: 5857 pFN21AE0525 5857 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2061 2861 3040 2061 Size: 800 179 4878 Fragments arranged by size: 4878 800 179 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3200 1937 Size: 1263 4594 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3920 5304 5572 1632 Size: 2288 1384 268 1917 Fragments arranged by size: 2288 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 3146 1166 Size: 1980 3877 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2190 1184 Size: 1006 4851 AlwNI CAG_nnn'CTG Cuts at: 2454 2464 2969 3600 5172 2454 Size: 10 505 631 1572 3139 Fragments arranged by size: 3139 1572 631 505 10 ApaI G_GGCC'C Cuts at: 2442 2442 Size: 5857 ApaLI G'TGCA_C Cuts at: 2219 3914 5070 2219 Size: 1695 1156 3006 Fragments arranged by size: 3006 1695 1156 ApoI r'AATT_y Cuts at: 1493 2414 3166 3392 3544 5653 1493 Size: 921 752 226 152 2109 1697 Fragments arranged by size: 2109 1697 921 752 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2902 2950 3182 1870 Size: 73 959 48 232 4545 Fragments arranged by size: 4545 959 232 73 48 BamHI G'GATC_C Cuts at: 3187 3554 3187 Size: 367 5490 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2712 2873 3178 4633 618 Size: 325 217 627 925 161 305 1455 1842 Fragments arranged by size: 1842 1455 925 627 325 305 217 161 BanII G_rGCy'C Cuts at: 1391 2442 3111 3176 1391 Size: 1051 669 65 4072 Fragments arranged by size: 4072 1051 669 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 928 Size: 971 261 4625 Fragments arranged by size: 4625 971 261 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2270 4468 136 Size: 107 122 71 631 605 598 2198 1525 Fragments arranged by size: 2198 1525 631 605 598 122 107 71 BglII A'GATC_T Cuts at: 5852 5852 Size: 5857 BlpI GC'TnA_GC Cuts at: 3024 3274 3024 Size: 250 5607 Bme1580I G_kGCm'C Cuts at: 1790 2223 2442 2876 3918 5074 1790 Size: 433 219 434 1042 1156 2573 Fragments arranged by size: 2573 1156 1042 434 433 219 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5857 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3588 4546 442 Size: 416 53 83 399 24 2171 958 1753 Fragments arranged by size: 2171 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2917 2917 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2131 4517 1239 Size: 812 80 2386 2579 Fragments arranged by size: 2579 2386 812 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2287 4520 882 Size: 617 788 2233 2219 Fragments arranged by size: 2233 2219 788 617 BsaAI yAC'GTr Cuts at: 493 1204 2224 3648 493 Size: 711 1020 1424 2702 Fragments arranged by size: 2702 1424 1020 711 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3664 4046 4681 275 Size: 53 83 186 936 2131 382 635 1451 Fragments arranged by size: 2131 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2564 4290 4688 4962 5109 5520 2564 Size: 1726 398 274 147 411 2901 Fragments arranged by size: 2901 1726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5632 Fragments arranged by size: 5632 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 1186 Size: 1021 4836 BseYI C'CCAG_C Cuts at: 5060 5556 5060 Size: 496 5361 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5284 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3307 4068 5096 1540 Size: 275 186 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 2791 2883 3111 3918 4003 5074 2223 Size: 568 92 228 807 85 1071 3006 Fragments arranged by size: 3006 1071 807 568 228 92 85 BsmI GAATG_Cn' Cuts at: 2042 3368 3461 2042 Size: 1326 93 4438 Fragments arranged by size: 4438 1326 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2197 2197 Size: 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2442 2791 2876 2883 3111 3176 Size: 399 433 219 349 85 7 228 65 Cuts at: 3176 3918 4003 5074 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 349 228 219 85 85 65 7 BspEI T'CCGG_A Cuts at: 2564 5520 2564 Size: 2956 2901 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2061 2861 3040 3075 3212 844 Size: 1217 800 179 35 137 3489 Fragments arranged by size: 3489 1217 800 179 137 35 BsrBI CCG'CTC Cuts at: 1394 1823 2059 3744 1394 Size: 429 236 1685 3507 Fragments arranged by size: 3507 1685 429 236 BsrDI GCAATG_nn' Cuts at: 65 1248 2334 2832 4352 4526 65 Size: 1183 1086 498 1520 174 1396 Fragments arranged by size: 1520 1396 1183 1086 498 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 2736 96 Size: 2640 3217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 2834 794 Size: 2040 3817 BstBI TT'CG_AA Cuts at: 2136 4661 2136 Size: 2525 3332 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstXI CCAn_nnnn'nTGG Cuts at: 2933 2933 Size: 5857 BstYI r'GATC_y Cuts at: 1972 3187 3554 3939 3956 5408 5494 5506 5852 Size: 1215 367 385 17 1452 86 12 346 Cuts at: 5852 1972 Size: 1977 Fragments arranged by size: 1977 1452 1215 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2547 513 Size: 552 939 543 3823 Fragments arranged by size: 3823 939 552 543 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 2842 3373 4167 4187 2842 Size: 531 794 20 4512 Fragments arranged by size: 4512 794 531 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DraIII CAC_nnn'GTG Cuts at: 2662 2662 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2775 3304 4193 Size: 54 1425 50 275 18 945 529 889 Cuts at: 4193 5822 8 Size: 1629 43 Fragments arranged by size: 1629 1425 945 889 529 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3304 1537 Size: 275 1492 4090 Fragments arranged by size: 4090 1492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3787 1353 Size: 2434 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4444 4818 4964 387 Size: 1064 138 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3109 3109 Size: 5857 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2131 3920 4517 5304 5572 1239 Size: 393 419 80 1789 597 787 268 1524 Fragments arranged by size: 1789 1524 787 597 419 393 268 80 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 2016 5004 1678 Size: 338 2988 2531 Fragments arranged by size: 2988 2531 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 2328 2360 4498 Size: 32 135 32 437 32 287 32 2138 Cuts at: 4498 4530 4572 4604 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2138 437 287 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2341 2628 3201 3447 669 Size: 988 281 403 287 573 246 3079 Fragments arranged by size: 3079 988 573 403 287 281 246 HindIII A'AGCT_T Cuts at: 748 2111 2130 2399 748 Size: 1363 19 269 4206 Fragments arranged by size: 4206 1363 269 19 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3153 4971 5155 1305 Size: 1848 1818 184 2007 Fragments arranged by size: 2007 1848 1818 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4035 Fragments arranged by size: 4035 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 2526 3146 3815 4174 4333 518 Size: 648 1320 40 620 669 359 159 2042 Fragments arranged by size: 2042 1320 669 648 620 359 159 40 MspA1I CmG'CkG Cuts at: 1606 2189 2200 3273 3950 5098 5343 1606 Size: 583 11 1073 677 1148 245 2120 Fragments arranged by size: 2120 1148 1073 677 583 245 11 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4366 Fragments arranged by size: 4366 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 3215 4760 1132 Size: 97 1986 1545 2229 Fragments arranged by size: 2229 1986 1545 97 PasI CC'CwG_GG Cuts at: 1348 2124 1348 Size: 776 5081 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 2482 1108 Size: 1374 4483 PfoI T'CCnGG_A Cuts at: 2355 2502 2355 Size: 147 5710 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PmlI CAC'GTG Cuts at: 2224 2224 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PspOMI G'GGCC_C Cuts at: 2438 2438 Size: 5857 PspXI vC'TCGA_Gb Cuts at: 2950 2950 Size: 5857 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2687 2719 2687 Size: 32 5825 PstI C_TGCA'G Cuts at: 830 2169 3209 830 Size: 1339 1040 3478 Fragments arranged by size: 3478 1339 1040 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 2200 2200 Size: 5857 SacI G_AGCT'C Cuts at: 3111 3111 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SfcI C'TryA_G Cuts at: 826 1045 2165 3133 3205 4340 5021 5212 826 Size: 219 1120 968 72 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 968 681 219 191 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 3184 1872 Size: 1312 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2950 3961 4862 5124 820 Size: 197 926 1007 1011 901 262 1553 Fragments arranged by size: 1553 1011 1007 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5857 StyI C'CwwG_G Cuts at: 513 1065 2004 2405 3296 513 Size: 552 939 401 891 3074 Fragments arranged by size: 3074 939 891 552 401 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2600 3884 3901 4054 4239 4684 325 Size: 1255 1020 1284 17 153 185 445 1498 Fragments arranged by size: 1498 1284 1255 1020 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2736 4103 5815 Size: 274 80 33 51 1443 759 1367 1712 Cuts at: 5815 96 Size: 138 Fragments arranged by size: 1712 1443 1367 759 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2519 2551 5817 5849 2519 Size: 32 3266 32 2527 Fragments arranged by size: 3266 2527 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2866 2866 Size: 5857 XhoI C'TCGA_G Cuts at: 1943 2950 1943 Size: 1007 4850 XmaI C'CCGG_G Cuts at: 1870 3182 1870 Size: 1312 4545 XmnI GAAnn'nnTTC Cuts at: 3986 3986 Size: 5857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI