################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbvI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HapII HincII HindII HindIII HinfI HpaI HpaII HphI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NciI NcoI NdeI NdeII NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI TseI Tth111I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AciI 484 CCGC CCGC(-3/-1) AcyI 443 GGCGCC G[GA]^CG[CT]C AseI 331 ATTAAT AT^TAAT AsnI 331 ATTAAT AT^TAAT BanI 443 GGCGCC G^G[CT][GA]CC BbeI 443 GGCGCC GGCGC^C BbvCI 478 CCTCAGC CCTCAGC(-5/-2) BcgI 432 GCAAGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 443 GGCGCC G[GA]^CG[CT]C BsmFI 100 GTCCC GGGAC(10/14) BssKI 4 CCAGG ^CC[ACGT]GG BstF5I 218 GGATG GGATG(2/0) BstNI 4 CCAGG CC^[AT]GG Cac8I 390 GCTAGC GC[ACGT]^[ACGT]GC CfoI 444 GCGC GCG^C EcoRI 405 GAATTC G^AATTC EcoRII 4 CCAGG ^CC[AT]GG Fnu4HI 483 GCCGC GC^[ACGT]GC FokI 218 GGATG GGATG(9/13) HaeII 443 GGCGCC [GA]GCGC^[CT] HgaI 19 GCGTC GACGC(5/10) HhaI 444 GCGC GCG^C Hin1I 443 GGCGCC G[GA]^CG[CT]C HinP1I 444 GCGC G^CGC ItaI 483 GCCGC GC^[ACGT]GC KasI 443 GGCGCC G^GCGCC MslI 381 CATATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 4 CCAGG CC^[AT]GG MwoI 161 GCACAGTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 443 GGCGCC GG^CGCC NheI 390 GCTAGC G^CTAGC NlaIII 195 CATG CATG^ PshBI 331 ATTAAT AT^TAAT ScrFI 4 CCAGG CC^[ACGT]GG SfaNI 416 GCATC GCATC(5/9) SfoI 443 GGCGCC GGC^GCC TaqI 131 TCGA T^CGA Tsp45I 33 GTCAC ^GT[GC]AC TthHB8I 131 TCGA T^CGA XbaI 177 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 76 AAATTT [GA]^AATT[CT] 405 GAATTC AluI 169 AGCT AG^CT 437 AGCT ApoI 76 AAATTT [GA]^AATT[CT] 405 GAATTC AvaII 283 GGACC G^G[AT]CC 369 GGACC BbsI 59 GAAGAC GAAGAC(2/6) 190 GAAGAC BpuAI 59 GAAGAC GAAGAC(2/6) 190 GAAGAC BsiYI 114 CCGACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 484 CCGCCCGAAGG BslI 114 CCGACTGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 484 CCGCCCGAAGG DdeI 46 CTGAG C^T[ACGT]AG 479 CTCAG HaeIII 139 GGCC GG^CC 230 GGCC MseI 269 TTAA T^TAA 332 TTAA NlaIV 369 GGACCC GG[ACGT]^[ACGT]CC 443 GGCGCC Tru9I 269 TTAA T^TAA 332 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### BfaI Cfr13I MaeI MaeIII MboII MnlI Sau96I Tsp509I TspRI