Restriction map for
pFN21AE3088
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmFI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstNI BstPI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI HphI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspA1I MvaI MwoI NaeI
NarI NcoI NdeI NgoMIV NlaIV
NotI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 168 CGCG CG^CG
AcsI 146 AAATTC [GA]^AATT[CT]
AflIII 408 ACATGT A^C[GA][CT]GT
AocI 567 CCTAAGG CC^T[ACGT]AGG
ApoI 146 AAATTC [GA]^AATT[CT]
Asp700I 142 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 548 CTCGAG C^[CT]CG[GA]G
AvaII 185 GGTCC G^G[AT]CC
BcnI 358 CCGGG CC^[GC]GG
BfaI 341 CTAG C^TAG
BsaAI 257 TACGTA [CT]AC^GT[GA]
BsmAI 171 GAGAC GTCTC(1/5)
BsoBI 548 CTCGAG C^[CT]CG[GA]G
BspHI 63 TCATGA T^CATGA
BspLU11I 408 ACATGT A^CATGT
BssKI 358 CCGGG ^CC[ACGT]GG
BstUI 168 CGCG CG^CG
BstYI 533 AGATCC [GA]^GATC[CT]
Bsu36I 567 CCTAAGG CC^T[ACGT]AGG
Cfr13I 185 GGTCC G^G[ACGT]CC
DsaI 181 CCACGG C^C[GA][CT]GG
EaeI 178 CGGCCA [CT]^GGCC[GA]
EarI 639 GAAGAG CTCTTC(1/4)
Eco81I 567 CCTAAGG CC^T[ACGT]AGG
HapII 358 CCGG C^CGG
HincII 412 GTTGAC GT[CT]^[GA]AC
HindII 412 GTTGAC GT[CT]^[GA]AC
HpaII 358 CCGG C^CGG
Ksp632I 639 GAAGAG CTCTTC(1/4)
MaeI 341 CTAG C^TAG
MaeIII 613 GTGAC ^GT[ACGT]AC
MfeI 11 CAATTG C^AATTG
MflI 533 AGATCC [GA]^GATC[CT]
MslI 64 CATGACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 358 CCGG C^CGG
MunI 11 CAATTG C^AATTG
MvnI 168 CGCG CG^CG
NciI 358 CCGGG CC^[GC]GG
NheI 340 GCTAGC G^CTAGC
NruI 167 TCGCGA TCG^CGA
NspI 408 ACATGT [GA]CATG^[CT]
PaeR7I 548 CTCGAG C^TCGAG
PleI 630 GAGTC GAGTC(4/5)
RcaI 63 TCATGA T^CATGA
SapI 639 GAAGAGC GCTCTTC(1/4)
Sau96I 185 GGTCC G^G[ACGT]CC
ScaI 592 AGTACT AGT^ACT
ScrFI 358 CCGGG CC^[ACGT]GG
SnaBI 257 TACGTA TAC^GTA
SspI 495 AATATT AAT^ATT
TaqI 549 TCGA T^CGA
Tsp45I 613 GTGAC ^GT[GC]AC
TspRI 621 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 549 TCGA T^CGA
XcmI 188 CCACGTTAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 548 CTCGAG C^TCGAG
XhoII 533 AGATCC [GA]^GATC[CT]
XmnI 142 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AluI 39 AGCT AG^CT
102 AGCT
AlwI 355 GATCC GGATC(4/5)
534 GATCC
Cac8I 340 GCTAGC GC[ACGT]^[ACGT]GC
402 GCAAGC
DdeI 568 CTAAG C^T[ACGT]AG
634 CTGAG
EcoT14I 287 CCTTGG C^C[AT][AT]GG
392 CCAAGG
HaeIII 179 GGCC GG^CC
285 GGCC
HgaI 31 GACGC GACGC(5/10)
583 GCGTC
MaeII 190 ACGT A^CGT
258 ACGT
MnlI 444 GAGG CCTC(7/6)
636 GAGG
SfaNI 70 GATGC GCATC(5/9)
580 GATGC
SmlI 525 CTTGAG C^T[CT][GA]AG
548 CTCGAG
StyI 287 CCTTGG C^C[AT][AT]GG
392 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AfaI BbvI BsaJI DpnII Fnu4HI
HinfI ItaI MboI MboII MseI
NdeII NlaIII RsaI Sau3AI TfiI
Tru9I TseI Tsp509I