################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BseRI BsgI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstYI BstZ17I CelII CpoI DraI DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HindIII HpaI KasI KpnI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp509I Tth111I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflIII 551 ACGTGT A^C[GA][CT]GT AgeI 559 ACCGGT A^CCGGT Alw44I 555 GTGCAC G^TGCAC AlwNI 331 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 255 CCTGAGG CC^T[ACGT]AGG ApaI 658 GGGCCC GGGCC^C ApaLI 555 GTGCAC G^TGCAC BanII 658 GGGCCC G[GA]GC[CT]^C BcgI 605 GCACCCCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 60 CTCCAG CTGGAG(16/14) BsaWI 559 ACCGGT [AT]^CCGG[AT] BsiEI 87 CGGCCG CG[GA][CT]^CG BsmBI 484 CGTCTC CGTCTC(1/5) BspDI 715 ATCGAT AT^CGAT BsrDI 590 GCAATG GCAATG(2/0) BstAPI 437 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 468 CCAGCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 255 CCTGAGG CC^T[ACGT]AGG ClaI 715 ATCGAT AT^CGAT DdeI 256 CTGAG C^T[ACGT]AG DraIII 634 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 87 CGGCCG C^GGCCG EarI 700 CTCTTC CTCTTC(1/4) EclXI 87 CGGCCG C^GGCCG Eco52I 87 CGGCCG C^GGCCG Eco81I 255 CCTGAGG CC^T[ACGT]AGG HaeII 696 GGCGCT [GA]GCGC^[CT] HincII 508 GTCAAC GT[CT]^[GA]AC HindII 508 GTCAAC GT[CT]^[GA]AC HinfI 135 GAATC G^A[ACGT]TC Ksp632I 700 CTCTTC CTCTTC(1/4) PaeR7I 48 CTCGAG C^TCGAG PflMI 633 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 559 ACCGGT A^CCGGT PshAI 532 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 412 CTGCAG CTGCA^G SapI 699 GCTCTTC GCTCTTC(1/4) SfcI 412 CTGCAG C^T[GA][CT]AG SgrAI 558 CACCGGTG C[GA]^CCGG[CT]G SmlI 48 CTCGAG C^T[CT][GA]AG TfiI 135 GAATC G^A[AT]TC Van91I 633 CCACGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 48 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 192 CGCG CG^CG 577 CGCG AcyI 42 GGCGTC G[GA]^CG[CT]C 482 GGCGTC AluI 199 AGCT AG^CT 359 AGCT AlwI 168 GATCC GGATC(4/5) 713 GGATC AspHI 555 GTGCAC G[AT]GC[AT]^C 565 GTGCTC BglI 22 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 575 GCCGCGGTGGC BsaHI 42 GGCGTC G[GA]^CG[CT]C 482 GGCGTC BsiHKAI 555 GTGCAC G[AT]GC[AT]^C 565 GTGCTC BsmAI 707 GAGAC GTCTC(1/5) 485 GTCTC BstUI 192 CGCG CG^CG 577 CGCG CfoI 471 GCGC GCG^C 697 GCGC DpnII 168 GATC ^GATC 714 GATC HgaI 43 GCGTC GACGC(5/10) 483 GCGTC HhaI 471 GCGC GCG^C 697 GCGC Hin1I 42 GGCGTC G[GA]^CG[CT]C 482 GGCGTC HinP1I 471 GCGC G^CGC 697 GCGC KspI 191 CCGCGG CCGC^GG 576 CCGCGG MboI 168 GATC ^GATC 714 GATC MboII 683 TCTTC GAAGA(8/7) 701 TCTTC MvnI 192 CGCG CG^CG 577 CGCG NdeII 168 GATC ^GATC 714 GATC NlaIII 172 CATG CATG^ 303 CATG PvuII 198 CAGCTG CAG^CTG 358 CAGCTG SacII 191 CCGCGG CCGC^GG 576 CCGCGG Sau3AI 168 GATC ^GATC 714 GATC SmaI 220 CCCGGG CCC^GGG 735 CCCGGG TaqI 49 TCGA T^CGA 716 TCGA TthHB8I 49 TCGA T^CGA 716 TCGA XcmI 62 CCAGCCCCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 440 CCAGATGCCAGGTGG XmaCI 220 CCCGGG C^CCGGG 735 CCCGGG XmaI 220 CCCGGG C^CCGGG 735 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AvaI AvaII BanI BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstNI Cac8I Cfr10I Cfr13I DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HpaII HphI ItaI MaeII MaeIII MnlI MslI MspA1I MspI MvaI MwoI NaeI NciI NgoMIV NlaIV Sau96I ScrFI SfaNI TseI Tsp45I TspRI