(Circular) (Six-base) MAPSORT of: pFN21AE3086.seq Check: 2944 from: 1 to: 5458 pFN21AE3086 5458 bp With 182 enzymes: SgfI * March 28, 2012 12:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3268 278 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2801 1937 Size: 864 4594 Acc65I G'GTAC_C Cuts at: 2779 2779 Size: 5458 AclI AA'CG_TT Cuts at: 1563 3585 3958 1563 Size: 2022 373 3063 Fragments arranged by size: 3063 2022 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 3521 4905 5173 1632 Size: 713 1176 1384 268 1917 Fragments arranged by size: 1917 1384 1176 713 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5261 AflIII A'CryG_T Cuts at: 1203 2556 4357 5334 1203 Size: 1353 1801 977 1327 Fragments arranged by size: 1801 1353 1327 977 AgeI A'CCGG_T Cuts at: 2564 4289 2564 Size: 1725 3733 AhdI GACnn_n'nnGTC Cuts at: 4187 4187 Size: 5458 AleI CACnn'nnGTG Cuts at: 1166 2240 2567 2696 1166 Size: 1074 327 129 3928 Fragments arranged by size: 3928 1074 327 129 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2258 2585 1184 Size: 1074 327 4057 Fragments arranged by size: 4057 1074 327 AlwNI CAG_nnn'CTG Cuts at: 2341 3201 4773 2341 Size: 860 1572 3026 Fragments arranged by size: 3026 1572 860 ApaI G_GGCC'C Cuts at: 2667 2667 Size: 5458 ApaLI G'TGCA_C Cuts at: 2560 3515 4671 2560 Size: 955 1156 3347 Fragments arranged by size: 3347 1156 955 ApoI r'AATT_y Cuts at: 1493 2767 2993 3145 5254 1493 Size: 1274 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 226 152 AseI AT'TA_AT Cuts at: 160 1032 4012 160 Size: 872 2980 1606 Fragments arranged by size: 2980 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 AvaI C'yCGr_G Cuts at: 1870 1943 2053 2225 2531 2694 2740 2783 1870 Size: 73 110 172 306 163 46 43 4545 Fragments arranged by size: 4545 306 172 163 110 73 46 43 BamHI G'GATC_C Cuts at: 2788 3155 2788 Size: 367 5091 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2165 2249 2441 2609 2779 Size: 325 217 627 378 84 192 168 170 Cuts at: 2779 4234 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 378 325 217 192 170 168 84 BanII G_rGCy'C Cuts at: 1391 2667 2777 1391 Size: 1276 110 4072 Fragments arranged by size: 4072 1276 110 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4487 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2599 2633 3649 3683 1903 Size: 34 662 34 1016 34 3678 Fragments arranged by size: 3678 1016 662 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3350 4560 3350 Size: 1210 4248 BclI T'GATC_A Cuts at: 1548 1548 Size: 5458 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 2586 4069 Size: 107 122 71 631 605 361 553 1483 Cuts at: 4069 136 Size: 1525 Fragments arranged by size: 1525 1483 631 605 553 361 122 107 71 BglII A'GATC_T Cuts at: 5453 5453 Size: 5458 BlpI GC'TnA_GC Cuts at: 2875 2875 Size: 5458 Bme1580I G_kGCm'C Cuts at: 1790 2170 2564 2612 2667 3519 4675 1790 Size: 380 394 48 55 852 1156 2573 Fragments arranged by size: 2573 1156 852 394 380 55 48 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5458 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3189 4147 442 Size: 416 53 83 399 24 1772 958 1753 Fragments arranged by size: 1772 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5458 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2720 2720 Size: 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2050 4118 1239 Size: 811 2068 2579 Fragments arranged by size: 2579 2068 811 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3583 4448 4746 3583 Size: 865 298 4295 Fragments arranged by size: 4295 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4121 882 Size: 617 2622 2219 Fragments arranged by size: 2622 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3249 493 Size: 711 2045 2702 Fragments arranged by size: 2702 2045 711 BsaBI GATnn'nnATC Cuts at: 5452 5452 Size: 5458 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 2488 3265 3647 Size: 53 83 186 936 515 440 777 382 Cuts at: 3647 4282 275 Size: 635 1451 Fragments arranged by size: 1451 936 777 635 515 440 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2564 3891 4289 4563 4710 5121 2564 Size: 1327 398 274 147 411 2901 Fragments arranged by size: 2901 1327 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 1178 Size: 30 165 30 861 30 4342 Fragments arranged by size: 4342 861 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5458 BseYI C'CCAG_C Cuts at: 2245 2331 2463 4661 5157 2245 Size: 86 132 2198 496 2546 Fragments arranged by size: 2546 2198 496 132 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4885 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2095 2908 3669 4697 1540 Size: 275 186 94 813 761 1028 2301 Fragments arranged by size: 2301 1028 813 761 275 186 94 BsiHKAI G_wGCw'C Cuts at: 2564 2574 3519 3604 4675 2564 Size: 10 945 85 1071 3347 Fragments arranged by size: 3347 1071 945 85 10 BsmI GAATG_Cn' Cuts at: 2969 3062 2969 Size: 93 5365 BsmBI CGTCTCn'nnnn_ Cuts at: 2495 2495 Size: 5458 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2170 2564 2574 2612 2667 2777 3519 Size: 399 380 394 10 38 55 110 742 Cuts at: 3519 3604 4675 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 380 110 85 55 38 10 BspEI T'CCGG_A Cuts at: 5121 5121 Size: 5458 BspHI T'CATG_A Cuts at: 3347 5077 3347 Size: 1730 3728 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2813 844 Size: 1969 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3345 1394 Size: 429 1522 3507 Fragments arranged by size: 3507 1522 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2602 3953 4127 65 Size: 1183 1354 1351 174 1396 Fragments arranged by size: 1396 1354 1351 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2438 2459 2564 4102 4289 1700 Size: 110 250 378 21 105 1538 187 2869 Fragments arranged by size: 2869 1538 378 250 187 110 105 21 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5458 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5458 BssSI C'ACGA_G Cuts at: 3518 4530 3518 Size: 1012 4446 BstAPI GCAn_nnn'nTGC Cuts at: 794 2448 794 Size: 1654 3804 BstBI TT'CG_AA Cuts at: 4262 4262 Size: 5458 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5458 BstXI CCAn_nnnn'nTGG Cuts at: 2480 2480 Size: 5458 BstYI r'GATC_y Cuts at: 1972 2788 3155 3540 3557 5009 5095 5107 5453 Size: 816 367 385 17 1452 86 12 346 Cuts at: 5453 1972 Size: 1977 Fragments arranged by size: 1977 1452 816 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2261 2261 Size: 5458 BtgI C'CryG_G Cuts at: 513 1065 2196 2238 2427 2581 2638 513 Size: 552 1131 42 189 154 57 3333 Fragments arranged by size: 3333 1131 552 189 154 57 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4719 Fragments arranged by size: 4719 683 56 BtsI GCAGTG_nn' Cuts at: 2408 2485 2974 3768 3788 2408 Size: 77 489 794 20 4078 Fragments arranged by size: 4078 794 489 77 20 ClaI AT'CG_AT Cuts at: 1554 2721 1554 Size: 1167 4291 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5423 DraI TTT'AAA Cuts at: 2762 2762 Size: 5458 DraIII CAC_nnn'GTG Cuts at: 2644 2644 Size: 5458 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4465 809 Size: 507 3149 1802 Fragments arranged by size: 3149 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2199 2457 Size: 54 1425 50 275 18 262 107 258 Cuts at: 2457 2671 2905 3794 5423 8 Size: 214 234 889 1629 43 Fragments arranged by size: 1629 1425 889 275 262 258 234 214 107 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2092 2905 1537 Size: 275 280 813 4090 Fragments arranged by size: 4090 813 280 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2711 3388 1353 Size: 1358 677 3423 Fragments arranged by size: 3423 1358 677 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4045 4419 4565 387 Size: 1064 138 2456 374 146 1280 Fragments arranged by size: 2456 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2050 2345 3521 4118 4905 5173 1239 Size: 393 418 295 1176 597 787 268 1524 Fragments arranged by size: 1524 1176 787 597 418 393 295 268 EcoO109I rG'GnC_Cy Cuts at: 2106 2629 2663 2664 2678 2106 Size: 523 34 1 14 4886 Fragments arranged by size: 4886 523 34 14 1 EcoRI G'AATT_C Cuts at: 2767 3145 2767 Size: 378 5080 FspI TGC'GCA Cuts at: 3964 3964 Size: 5458 HaeII r_GCGC'y Cuts at: 1678 2705 4605 1678 Size: 1027 1900 2531 Fragments arranged by size: 2531 1900 1027 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 2703 2735 4099 Size: 32 135 32 692 32 407 32 1364 Cuts at: 4099 4131 4173 4205 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1364 692 407 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2515 2802 3048 669 Size: 988 281 577 287 246 3079 Fragments arranged by size: 3079 988 577 287 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5458 HpaI GTT'AAC Cuts at: 3048 3048 Size: 5458 KpnI G_GTAC'C Cuts at: 2783 2783 Size: 5458 MfeI C'AATT_G Cuts at: 3057 3057 Size: 5458 MluI A'CGCG_T Cuts at: 5334 5334 Size: 5458 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4572 4756 1305 Size: 3267 184 2007 Fragments arranged by size: 3267 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3636 Fragments arranged by size: 3636 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 2567 2696 3416 3775 3934 518 Size: 648 1074 327 129 720 359 159 2042 Fragments arranged by size: 2042 1074 720 648 359 327 159 129 MspA1I CmG'CkG Cuts at: 1606 2198 2205 2365 2583 2874 3551 4699 4944 Size: 592 7 160 218 291 677 1148 245 Cuts at: 4944 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 592 291 245 218 160 7 NaeI GCC'GGC Cuts at: 2062 2440 2461 2062 Size: 378 21 5059 Fragments arranged by size: 5059 378 21 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4906 NdeI CA'TA_TG Cuts at: 387 387 Size: 5458 NgoMIV G'CCGG_C Cuts at: 2060 2438 2459 2060 Size: 378 21 5059 Fragments arranged by size: 5059 378 21 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5458 NotI GC'GGCC_GC Cuts at: 2905 2905 Size: 5458 NspI r_CATG'y Cuts at: 1132 1229 2816 4361 1132 Size: 97 1587 1545 2229 Fragments arranged by size: 2229 1587 1545 97 PasI CC'CwG_GG Cuts at: 1348 2659 1348 Size: 1311 4147 PciI A'CATG_T Cuts at: 4357 4357 Size: 5458 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 1108 Size: 1536 3922 PmeI GTTT'AAAC Cuts at: 2762 2762 Size: 5458 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3527 3559 5040 5072 3527 Size: 32 1481 32 3913 Fragments arranged by size: 3913 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2541 1613 Size: 928 4530 PsiI TTA'TAA Cuts at: 3028 3028 Size: 5458 PspOMI G'GGCC_C Cuts at: 2663 2663 Size: 5458 PspXI vC'TCGA_Gb Cuts at: 2053 2053 Size: 5458 PstI C_TGCA'G Cuts at: 830 2421 2810 830 Size: 1591 389 3478 Fragments arranged by size: 3478 1591 389 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5458 PvuII CAG'CTG Cuts at: 2205 2365 2205 Size: 160 5298 SacII CC_GC'GG Cuts at: 2199 2584 2199 Size: 385 5073 SalI G'TCGA_C Cuts at: 1936 2800 1936 Size: 864 4594 SapI GCTCTTCn'nnn_ Cuts at: 2711 2711 Size: 5458 SbfI CC_TGCA'GG Cuts at: 2810 2810 Size: 5458 ScaI AGT'ACT Cuts at: 3706 5418 3706 Size: 1712 3746 SfcI C'TryA_G Cuts at: 826 1045 2417 2806 3941 4622 4813 826 Size: 219 1372 389 1135 681 191 1471 Fragments arranged by size: 1471 1372 1135 681 389 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5458 SgrAI Cr'CCGG_yG Cuts at: 1700 2564 1700 Size: 864 4594 SmaI CCC'GGG Cuts at: 1872 2227 2742 2785 1872 Size: 355 515 43 4545 Fragments arranged by size: 4545 515 355 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 3562 4463 4725 820 Size: 197 926 110 1509 901 262 1553 Fragments arranged by size: 1553 1509 926 901 262 197 110 SnaBI TAC'GTA Cuts at: 493 3249 493 Size: 2756 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5458 SphI G_CATG'C Cuts at: 1132 2816 1132 Size: 1684 3774 SspI AAT'ATT Cuts at: 5 52 3382 5 Size: 47 3330 2081 Fragments arranged by size: 3330 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5458 StyI C'CwwG_G Cuts at: 513 1065 2897 513 Size: 552 1832 3074 Fragments arranged by size: 3074 1832 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2237 3485 3502 3655 3840 4285 325 Size: 1255 657 1248 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1248 657 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3704 5416 96 Size: 274 80 33 51 1443 1727 1712 138 Fragments arranged by size: 1727 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2710 2742 5418 5450 2710 Size: 32 2676 32 2718 Fragments arranged by size: 2718 2676 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5458 XbaI T'CTAG_A Cuts at: 2794 2794 Size: 5458 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 2452 2074 Size: 378 5080 XhoI C'TCGA_G Cuts at: 1943 2053 1943 Size: 110 5348 XmaI C'CCGG_G Cuts at: 1870 2225 2740 2783 1870 Size: 355 515 43 4545 Fragments arranged by size: 4545 515 355 43 XmnI GAAnn'nnTTC Cuts at: 3587 3587 Size: 5458 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3266 276 Size: 53 83 186 936 1732 2468 Fragments arranged by size: 2468 1732 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI