Restriction map for
pFN21AB0420
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII BalI BamHI BanI BbeI
BbrPI BciVI BclI BcnI BglI
BlpI Bpu1102I BsaAI BsaI BseAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BssHII BssSI
BstBI BstEII BstPI CelII ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2008 GTATAC GT^[AC][GT]AC
AflII 703 CTTAAG C^TTAAG
AgeI 222 ACCGGT A^CCGGT
AocI 2000 CCTCAGG CC^T[ACGT]AGG
Asp700I 954 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2263 CCTAGG C^CTAGG
BanII 1406 GAGCTC G[GA]GC[CT]^C
BbvCI 1174 GCTGAGG CCTCAGC(-5/-2)
BcgI 41 CGAAGCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 703 CTTAAG C^TTAAG
BlnI 2263 CCTAGG C^CTAGG
BpmI 1475 CTCCAG CTGGAG(16/14)
BsaBI 1102 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 997 CGACCG CG[GA][CT]^CG
BsmAI 867 GAGAC GTCTC(1/5)
Bsp1407I 2052 TGTACA T^GTACA
BspHI 2376 TCATGA T^CATGA
BsrGI 2052 TGTACA T^GTACA
Bst1107I 2008 GTATAC GTA^TAC
BstXI 125 CCACCGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2008 GTATAC GTA^TAC
Bsu36I 2000 CCTCAGG CC^T[ACGT]AGG
DraI 928 TTTAAA TTT^AAA
EaeI 2303 TGGCCG [CT]^GGCC[GA]
Eco81I 2000 CCTCAGG CC^T[ACGT]AGG
HaeII 27 GGCGCT [GA]GCGC^[CT]
MamI 1102 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 1693 ACATGC [GA]CATG^[CT]
PinAI 222 ACCGGT A^CCGGT
PshAI 1537 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2376 TCATGA T^CATGA
SacI 1406 GAGCTC GAGCT^C
SapI 1163 GCTCTTC GCTCTTC(1/4)
SmlI 703 CTTAAG C^T[CT][GA]AG
SspBI 2052 TGTACA T^GTACA
StuI 1588 AGGCCT AGG^CCT
XbaI 741 TCTAGA T^CTAGA
XmnI 954 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 23 CGCG CG^CG
1381 CGCG
AcsI 277 GAATTT [GA]^AATT[CT]
1814 AAATTC
AcyI 34 GACGCC G[GA]^CG[CT]C
1987 GGCGTC
Alw44I 1895 GTGCAC G^TGCAC
2129 GTGCAC
AlwNI 1777 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
2173 CAGGTTCTG
ApaLI 1895 GTGCAC G^TGCAC
2129 GTGCAC
ApoI 277 GAATTT [GA]^AATT[CT]
1814 AAATTC
BglII 717 AGATCT A^GATCT
1101 AGATCT
BsaHI 34 GACGCC G[GA]^CG[CT]C
1987 GGCGTC
BsaWI 127 ACCGGA [AT]^CCGG[AT]
222 ACCGGT
BsgI 82 CTGCAC GTGCAG(16/14)
1076 CTGCAC
BsrDI 407 CATTGC GCAATG(2/0)
943 GCAATG
BsrFI 51 GCCGGT [GA]^CCGG[CT]
222 ACCGGT
BstAPI 1735 GCACACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1897 GCACAGTTTGC
BstUI 23 CGCG CG^CG
1381 CGCG
Cfr10I 51 GCCGGT [GA]^CCGG[CT]
222 ACCGGT
DraII 1684 GGGACCC [GA]G^G[ACGT]CC[CT]
2333 AGGTCCC
EcoO109I 1684 GGGACCC [GA]G^G[ACGT]CC[CT]
2333 AGGTCCC
FauI 24 GCGGG CCCGC(4/6)
168 GCGGG
HgaI 34 GACGC GACGC(5/10)
1988 GCGTC
Hin1I 34 GACGCC G[GA]^CG[CT]C
1987 GGCGTC
MvnI 23 CGCG CG^CG
1381 CGCG
PpuMI 1684 GGGACCC [GA]G^G[AT]CC[CT]
2333 AGGTCCC
PstI 469 CTGCAG CTGCA^G
2309 CTGCAG
ScaI 1232 AGTACT AGT^ACT
2032 AGTACT
SpeI 1276 ACTAGT A^CTAGT
1551 ACTAGT
XcmI 1149 CCAGGTTTTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1344 CCAAAGGTGTATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbsI BbvI BfaI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII