(Circular) (Six-base) MAPSORT of: pFN21AB0420.seq Check: 4452 from: 1 to: 7144 pFN21AB0420 7144 bp With 182 enzymes: SgfI * March 28, 2012 12:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2182 2182 Size: 7144 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4954 278 Size: 53 83 186 936 3418 2468 Fragments arranged by size: 3418 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4014 4487 1937 Size: 2077 473 4594 Fragments arranged by size: 4594 2077 473 Acc65I G'GTAC_C Cuts at: 4465 4465 Size: 7144 AclI AA'CG_TT Cuts at: 1563 5271 5644 1563 Size: 3708 373 3063 Fragments arranged by size: 3708 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 2382 2565 5207 6591 6859 1632 Size: 521 229 183 2642 1384 268 1917 Fragments arranged by size: 2642 1917 1384 521 268 229 183 AflII C'TTAA_G Cuts at: 820 1017 2708 820 Size: 197 1691 5256 Fragments arranged by size: 5256 1691 197 AflIII A'CryG_T Cuts at: 1203 6043 7020 1203 Size: 4840 977 1327 Fragments arranged by size: 4840 1327 977 AgeI A'CCGG_T Cuts at: 2227 5975 2227 Size: 3748 3396 AhdI GACnn_n'nnGTC Cuts at: 5873 5873 Size: 7144 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7144 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7144 AlwNI CAG_nnn'CTG Cuts at: 3787 4183 4887 6459 3787 Size: 396 704 1572 4472 Fragments arranged by size: 4472 1572 704 396 ApaLI G'TGCA_C Cuts at: 3900 4134 5201 6357 3900 Size: 234 1067 1156 4687 Fragments arranged by size: 4687 1156 1067 234 ApoI r'AATT_y Cuts at: 1493 2282 3819 4453 4679 4831 6940 1493 Size: 789 1537 634 226 152 2109 1697 Fragments arranged by size: 2109 1697 1537 789 634 226 152 AseI AT'TA_AT Cuts at: 160 1032 5698 160 Size: 872 4666 1606 Fragments arranged by size: 4666 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7144 AvaI C'yCGr_G Cuts at: 1870 1943 4469 1870 Size: 73 2526 4545 Fragments arranged by size: 4545 2526 73 AvrII C'CTAG_G Cuts at: 4268 4268 Size: 7144 BamHI G'GATC_C Cuts at: 4474 4841 4474 Size: 367 6777 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4465 5920 618 Size: 325 217 627 2678 1455 1842 Fragments arranged by size: 2678 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3415 4463 1391 Size: 2024 1048 4072 Fragments arranged by size: 4072 2024 1048 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3085 3192 3379 928 Size: 971 1186 107 187 4693 Fragments arranged by size: 4693 1186 971 187 107 BbvCI CC'TCA_GC Cuts at: 3180 3180 Size: 7144 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2035 2069 5335 5369 1903 Size: 34 98 34 3266 34 3678 Fragments arranged by size: 3678 3266 98 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5036 6246 5036 Size: 1210 5934 BclI T'GATC_A Cuts at: 1548 1548 Size: 7144 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5755 136 Size: 107 122 71 631 605 4083 1525 Fragments arranged by size: 4083 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2722 3106 7139 2722 Size: 384 4033 2727 Fragments arranged by size: 4033 2727 384 BlpI GC'TnA_GC Cuts at: 4561 4561 Size: 7144 Bme1580I G_kGCm'C Cuts at: 1790 3904 4138 5205 6361 1790 Size: 2114 234 1067 1156 2573 Fragments arranged by size: 2573 2114 1156 1067 234 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7144 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4875 5833 442 Size: 416 53 83 399 24 3458 958 1753 Fragments arranged by size: 3458 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7144 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3465 5804 1239 Size: 2226 2339 2579 Fragments arranged by size: 2579 2339 2226 Bpu10I CC'TnA_GC Cuts at: 3180 3180 Size: 7144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5269 6134 6432 5269 Size: 865 298 5981 Fragments arranged by size: 5981 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5807 882 Size: 617 4308 2219 Fragments arranged by size: 4308 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4935 493 Size: 711 3731 2702 Fragments arranged by size: 3731 2702 711 BsaBI GATnn'nnATC Cuts at: 3111 7138 3111 Size: 4027 3117 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 3993 4951 5333 Size: 53 83 186 936 507 1953 958 382 Cuts at: 5333 5968 275 Size: 635 1451 Fragments arranged by size: 1953 1451 958 936 635 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2132 2227 5577 5975 6249 6396 6807 2132 Size: 95 3350 398 274 147 411 2469 Fragments arranged by size: 3350 2469 411 398 274 147 95 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6919 Fragments arranged by size: 6919 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2438 3237 1186 Size: 979 273 799 5093 Fragments arranged by size: 5093 979 799 273 BseYI C'CCAG_C Cuts at: 3128 6347 6843 3128 Size: 3219 496 3429 Fragments arranged by size: 3429 3219 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 3066 1163 Size: 573 336 994 5241 Fragments arranged by size: 5241 994 573 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3005 4594 5355 6383 1540 Size: 275 186 1004 1589 761 1028 2301 Fragments arranged by size: 2301 1589 1028 1004 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3415 3904 4138 5205 5290 6361 3415 Size: 489 234 1067 85 1071 4198 Fragments arranged by size: 4198 1071 1067 489 234 85 BsmI GAATG_Cn' Cuts at: 4655 4748 4655 Size: 93 7051 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3415 3904 4138 4463 5205 5290 6361 Size: 399 1625 489 234 325 742 85 1071 Cuts at: 6361 1391 Size: 2174 Fragments arranged by size: 2174 1625 1071 742 489 399 325 234 85 BspEI T'CCGG_A Cuts at: 6807 6807 Size: 7144 BspHI T'CATG_A Cuts at: 4381 5033 6763 4381 Size: 652 1730 4762 Fragments arranged by size: 4762 1730 652 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 2182 4070 4499 844 Size: 1250 88 1888 429 3489 Fragments arranged by size: 3489 1888 1250 429 88 BsrBI CCG'CTC Cuts at: 1394 1823 5031 1394 Size: 429 3208 3507 Fragments arranged by size: 3507 3208 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2411 2955 5639 5813 65 Size: 1183 1163 544 2684 174 1396 Fragments arranged by size: 2684 1396 1183 1163 544 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 2227 5788 5975 1700 Size: 110 246 171 3561 187 2869 Fragments arranged by size: 3561 2869 246 187 171 110 BsrGI T'GTAC_A Cuts at: 96 4057 96 Size: 3961 3183 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7144 BssSI C'ACGA_G Cuts at: 5204 6216 5204 Size: 1012 6132 BstAPI GCAn_nnn'nTGC Cuts at: 794 3746 3908 794 Size: 2952 162 4030 Fragments arranged by size: 4030 2952 162 BstBI TT'CG_AA Cuts at: 5948 5948 Size: 7144 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7144 BstXI CCAn_nnnn'nTGG Cuts at: 2137 2137 Size: 7144 BstYI r'GATC_y Cuts at: 1972 2074 2722 3106 4474 4841 5226 5243 6695 Size: 102 648 384 1368 367 385 17 1452 Cuts at: 6695 6781 6793 7139 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1368 648 385 384 367 346 102 86 17 12 BstZ17I GTA'TAC Cuts at: 4015 4015 Size: 7144 Bsu36I CC'TnA_GG Cuts at: 4006 4006 Size: 7144 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6592 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4445 487 Size: 683 56 3219 3186 Fragments arranged by size: 3219 3186 683 56 BtsI GCAGTG_nn' Cuts at: 2458 3083 3736 4660 5454 5474 2458 Size: 625 653 924 794 20 4128 Fragments arranged by size: 4128 924 794 653 625 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7144 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7109 DraI TTT'AAA Cuts at: 2935 4448 2935 Size: 1513 5631 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6151 809 Size: 507 4835 1802 Fragments arranged by size: 4835 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4308 4591 5480 Size: 54 1425 50 275 18 2478 283 889 Cuts at: 5480 7109 8 Size: 1629 43 Fragments arranged by size: 2478 1629 1425 889 283 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4591 1537 Size: 275 2779 4090 Fragments arranged by size: 4090 2779 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2142 2201 2758 3175 5074 1353 Size: 789 59 557 417 1899 3423 Fragments arranged by size: 3423 1899 789 557 417 59 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5731 6105 6251 387 Size: 1064 138 4142 374 146 1280 Fragments arranged by size: 4142 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3413 3413 Size: 7144 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2382 2565 3465 5207 5804 6591 Size: 393 521 229 183 900 1742 597 787 Cuts at: 6591 6859 1239 Size: 268 1524 Fragments arranged by size: 1742 1524 900 787 597 521 393 268 229 183 EcoO109I rG'GnC_Cy Cuts at: 3690 4339 3690 Size: 649 6495 EcoRI G'AATT_C Cuts at: 4453 4831 4453 Size: 378 6766 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3011 3011 Size: 7144 FspI TGC'GCA Cuts at: 5650 5650 Size: 7144 HaeII r_GCGC'y Cuts at: 1678 2036 6291 1678 Size: 358 4255 2531 Fragments arranged by size: 4255 2531 358 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2675 2707 2837 2869 3620 Size: 32 135 32 1103 32 130 32 751 Cuts at: 3620 3652 5785 5817 5859 5891 1373 Size: 32 2133 32 42 32 2626 Fragments arranged by size: 2626 2133 1103 751 135 130 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4488 4734 669 Size: 988 281 2550 246 3079 Fragments arranged by size: 3079 2550 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7144 HpaI GTT'AAC Cuts at: 4734 4734 Size: 7144 KpnI G_GTAC'C Cuts at: 4469 4469 Size: 7144 MfeI C'AATT_G Cuts at: 4743 4743 Size: 7144 MluI A'CGCG_T Cuts at: 7020 7020 Size: 7144 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 3025 6258 6442 1305 Size: 831 889 3233 184 2007 Fragments arranged by size: 3233 2007 889 831 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5322 Fragments arranged by size: 5322 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2135 3577 3618 5102 5461 5620 518 Size: 648 969 1442 41 1484 359 159 2042 Fragments arranged by size: 2042 1484 1442 969 648 359 159 41 MspA1I CmG'CkG Cuts at: 1606 2053 2067 2173 3053 4313 4560 5237 6385 Size: 447 14 106 880 1260 247 677 1148 Cuts at: 6385 6630 1606 Size: 245 2120 Fragments arranged by size: 2120 1260 1148 880 677 447 247 245 106 14 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6592 NdeI CA'TA_TG Cuts at: 387 387 Size: 7144 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7144 NotI GC'GGCC_GC Cuts at: 4591 4591 Size: 7144 NspI r_CATG'y Cuts at: 1132 1229 3702 4502 6047 1132 Size: 97 2473 800 1545 2229 Fragments arranged by size: 2473 2229 1545 800 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7144 PciI A'CATG_T Cuts at: 6043 6043 Size: 7144 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7144 PmeI GTTT'AAAC Cuts at: 4448 4448 Size: 7144 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5213 5245 6726 6758 5213 Size: 32 1481 32 5599 Fragments arranged by size: 5599 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3690 4339 3690 Size: 649 6495 PshAI GACnn'nnGTC Cuts at: 1613 3546 1613 Size: 1933 5211 PsiI TTA'TAA Cuts at: 4714 4714 Size: 7144 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3718 3750 4350 4382 3718 Size: 32 600 32 6480 Fragments arranged by size: 6480 600 32 32 PstI C_TGCA'G Cuts at: 830 2478 4318 4496 830 Size: 1648 1840 178 3478 Fragments arranged by size: 3478 1840 1648 178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7144 SacI G_AGCT'C Cuts at: 3415 3415 Size: 7144 SalI G'TCGA_C Cuts at: 1936 4486 1936 Size: 2550 4594 SanDI GG'GwC_CC Cuts at: 3690 3690 Size: 7144 SapI GCTCTTCn'nnn_ Cuts at: 3175 3175 Size: 7144 SbfI CC_TGCA'GG Cuts at: 4496 4496 Size: 7144 ScaI AGT'ACT Cuts at: 3239 4039 5392 7104 3239 Size: 800 1353 1712 3279 Fragments arranged by size: 3279 1712 1353 800 SfcI C'TryA_G Cuts at: 826 1045 2474 3640 4314 4356 4492 5627 6308 Size: 219 1429 1166 674 42 136 1135 681 Cuts at: 6308 6499 826 Size: 191 1471 Fragments arranged by size: 1471 1429 1166 1135 681 674 219 191 136 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7144 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7144 SmaI CCC'GGG Cuts at: 1872 4471 1872 Size: 2599 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2708 5248 6149 6411 820 Size: 197 926 765 2540 901 262 1553 Fragments arranged by size: 2540 1553 926 901 765 262 197 SnaBI TAC'GTA Cuts at: 493 4935 493 Size: 4442 2702 SpeI A'CTAG_T Cuts at: 152 3281 3556 152 Size: 3129 275 3740 Fragments arranged by size: 3740 3129 275 SphI G_CATG'C Cuts at: 1132 4502 1132 Size: 3370 3774 SspI AAT'ATT Cuts at: 5 52 2251 2534 4108 5068 5 Size: 47 2199 283 1574 960 2081 Fragments arranged by size: 2199 2081 1574 960 283 47 StuI AGG'CCT Cuts at: 1517 3595 1517 Size: 2078 5066 StyI C'CwwG_G Cuts at: 513 1065 3275 4268 4335 4370 4583 513 Size: 552 2210 993 67 35 213 3074 Fragments arranged by size: 3074 2210 993 552 213 67 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3019 5171 5188 5341 5526 5971 325 Size: 1255 1439 2152 17 153 185 445 1498 Fragments arranged by size: 2152 1498 1439 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3237 4037 4057 Size: 274 80 33 51 1443 1260 800 20 Cuts at: 4057 5390 7102 96 Size: 1333 1712 138 Fragments arranged by size: 1712 1443 1333 1260 800 274 138 80 51 33 20 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2605 2637 3969 4001 4264 4296 7104 7136 2605 Size: 32 1332 32 263 32 2808 32 2613 Fragments arranged by size: 2808 2613 1332 263 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7144 XbaI T'CTAG_A Cuts at: 2746 4480 2746 Size: 1734 5410 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3161 3356 3161 Size: 195 6949 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7144 XmaI C'CCGG_G Cuts at: 1870 4469 1870 Size: 2599 4545 XmnI GAAnn'nnTTC Cuts at: 2963 5273 2963 Size: 2310 4834 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4952 276 Size: 53 83 186 936 3418 2468 Fragments arranged by size: 3418 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI