Restriction map for
pFN21AB0420
(Linear) (Six-base) MAPSORT of: KIBB0420.seq Check: 9578 from: 1 to: 2439
KIBB0420.orf
With 182 enzymes: SgfI *
March 22, 2012 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 177 2439
Size: 177 2262
AccI GT'mk_AC
Cuts at: 0 2009 2439
Size: 2009 430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 377 560 2439
Size: 148 229 183 1879
Fragments arranged by size:
1879 229 183 148
AflII C'TTAA_G
Cuts at: 0 703 2439
Size: 703 1736
AgeI A'CCGG_T
Cuts at: 0 222 2439
Size: 222 2217
AlwNI CAG_nnn'CTG
Cuts at: 0 1782 2178 2439
Size: 1782 396 261
Fragments arranged by size:
1782 396 261
ApaLI G'TGCA_C
Cuts at: 0 1895 2129 2439
Size: 1895 234 310
Fragments arranged by size:
1895 310 234
ApoI r'AATT_y
Cuts at: 0 277 1814 2439
Size: 277 1537 625
Fragments arranged by size:
1537 625 277
AvrII C'CTAG_G
Cuts at: 0 2263 2439
Size: 2263 176
BanII G_rGCy'C
Cuts at: 0 1410 2439
Size: 1410 1029
BbsI GAAGACnn'nnnn_
Cuts at: 0 1080 1187 1374 2439
Size: 1080 107 187 1065
Fragments arranged by size:
1080 1065 187 107
BbvCI CC'TCA_GC
Cuts at: 0 1175 2439
Size: 1175 1264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 30 64 2439
Size: 30 34 2375
Fragments arranged by size:
2375 34 30
BglII A'GATC_T
Cuts at: 0 717 1101 2439
Size: 717 384 1338
Fragments arranged by size:
1338 717 384
Bme1580I G_kGCm'C
Cuts at: 0 1899 2133 2439
Size: 1899 234 306
Fragments arranged by size:
1899 306 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1460 2439
Size: 1460 979
Bpu10I CC'TnA_GC
Cuts at: 0 1175 2439
Size: 1175 1264
BsaBI GATnn'nnATC
Cuts at: 0 1106 2439
Size: 1106 1333
BsaHI Gr'CG_yC
Cuts at: 0 35 1988 2439
Size: 35 1953 451
Fragments arranged by size:
1953 451 35
BsaWI w'CCGG_w
Cuts at: 0 127 222 2439
Size: 127 95 2217
Fragments arranged by size:
2217 127 95
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 433 1232 2439
Size: 160 273 799 1207
Fragments arranged by size:
1207 799 273 160
BseYI C'CCAG_C
Cuts at: 0 1123 2439
Size: 1123 1316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1061 2439
Size: 67 994 1378
Fragments arranged by size:
1378 994 67
BsiEI CG_ry'CG
Cuts at: 0 1000 2439
Size: 1000 1439
BsiHKAI G_wGCw'C
Cuts at: 0 1410 1899 2133 2439
Size: 1410 489 234 306
Fragments arranged by size:
1410 489 306 234
Bsp1286I G_dGCh'C
Cuts at: 0 1410 1899 2133 2439
Size: 1410 489 234 306
Fragments arranged by size:
1410 489 306 234
BspHI T'CATG_A
Cuts at: 0 2376 2439
Size: 2376 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 177 2065 2439
Size: 89 88 1888 374
Fragments arranged by size:
1888 374 89 88
BsrDI GCAATG_nn'
Cuts at: 0 406 950 2439
Size: 406 544 1489
Fragments arranged by size:
1489 544 406
BsrFI r'CCGG_y
Cuts at: 0 51 222 2439
Size: 51 171 2217
Fragments arranged by size:
2217 171 51
BsrGI T'GTAC_A
Cuts at: 0 2052 2439
Size: 2052 387
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1741 1903 2439
Size: 1741 162 536
Fragments arranged by size:
1741 536 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 2439
Size: 132 2307
BstYI r'GATC_y
Cuts at: 0 69 717 1101 2439
Size: 69 648 384 1338
Fragments arranged by size:
1338 648 384 69
BstZ17I GTA'TAC
Cuts at: 0 2010 2439
Size: 2010 429
Bsu36I CC'TnA_GG
Cuts at: 0 2001 2439
Size: 2001 438
BtsI GCAGTG_nn'
Cuts at: 0 453 1078 1731 2439
Size: 453 625 653 708
Fragments arranged by size:
708 653 625 453
DraI TTT'AAA
Cuts at: 0 930 2439
Size: 930 1509
EaeI y'GGCC_r
Cuts at: 0 2303 2439
Size: 2303 136
EarI CTCTTCn'nnn_
Cuts at: 0 137 196 753 1170 2439
Size: 137 59 557 417 1269
Fragments arranged by size:
1269 557 417 137 59
EcoICRI GAG'CTC
Cuts at: 0 1408 2439
Size: 1408 1031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 377 560 1460 2439
Size: 148 229 183 900 979
Fragments arranged by size:
979 900 229 183 148
EcoO109I rG'GnC_Cy
Cuts at: 0 1685 2334 2439
Size: 1685 649 105
Fragments arranged by size:
1685 649 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1006 2439
Size: 1006 1433
HaeII r_GCGC'y
Cuts at: 0 31 2439
Size: 31 2408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 832 864 1615 1647 2439
Size: 670 32 130 32 751 32 792
Fragments arranged by size:
792 751 670 130 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1020 2439
Size: 131 889 1419
Fragments arranged by size:
1419 889 131
MslI CAynn'nnrTG
Cuts at: 0 130 1572 1613 2439
Size: 130 1442 41 826
Fragments arranged by size:
1442 826 130 41
MspA1I CmG'CkG
Cuts at: 0 48 62 168 1048 2308 2439
Size: 48 14 106 880 1260 131
Fragments arranged by size:
1260 880 131 106 48 14
NspI r_CATG'y
Cuts at: 0 1697 2439
Size: 1697 742
PpuMI rG'GwC_Cy
Cuts at: 0 1685 2334 2439
Size: 1685 649 105
Fragments arranged by size:
1685 649 105
PshAI GACnn'nnGTC
Cuts at: 0 1541 2439
Size: 1541 898
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1713 1745 2345 2377 2439
Size: 1713 32 600 32 62
Fragments arranged by size:
1713 600 62 32 32
PstI C_TGCA'G
Cuts at: 0 473 2313 2439
Size: 473 1840 126
Fragments arranged by size:
1840 473 126
SacI G_AGCT'C
Cuts at: 0 1410 2439
Size: 1410 1029
SanDI GG'GwC_CC
Cuts at: 0 1685 2439
Size: 1685 754
SapI GCTCTTCn'nnn_
Cuts at: 0 1170 2439
Size: 1170 1269
ScaI AGT'ACT
Cuts at: 0 1234 2034 2439
Size: 1234 800 405
Fragments arranged by size:
1234 800 405
SfcI C'TryA_G
Cuts at: 0 469 1635 2309 2351 2439
Size: 469 1166 674 42 88
Fragments arranged by size:
1166 674 469 88 42
SmlI C'TyrA_G
Cuts at: 0 703 2439
Size: 703 1736
SpeI A'CTAG_T
Cuts at: 0 1276 1551 2439
Size: 1276 275 888
Fragments arranged by size:
1276 888 275
SspI AAT'ATT
Cuts at: 0 246 529 2103 2439
Size: 246 283 1574 336
Fragments arranged by size:
1574 336 283 246
StuI AGG'CCT
Cuts at: 0 1590 2439
Size: 1590 849
StyI C'CwwG_G
Cuts at: 0 1270 2263 2330 2365 2439
Size: 1270 993 67 35 74
Fragments arranged by size:
1270 993 74 67 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1014 2439
Size: 1014 1425
TatI w'GTAC_w
Cuts at: 0 1232 2032 2052 2439
Size: 1232 800 20 387
Fragments arranged by size:
1232 800 387 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 600 632 1964 1996 2259 2291 2439
Size: 600 32 1332 32 263 32 148
Fragments arranged by size:
1332 600 263 148 32 32 32
XbaI T'CTAG_A
Cuts at: 0 741 2439
Size: 741 1698
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1156 1351 2439
Size: 1156 195 1088
Fragments arranged by size:
1156 1088 195
XmnI GAAnn'nnTTC
Cuts at: 0 958 2439
Size: 958 1481
Enzymes that do cut:
AarI AccI AcuI AflII AgeI AlwNI ApaLI ApoI
AvrII BanII BbsI BbvCI BcgI BglII Bme1580I BpmI
Bpu10I BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI BstZ17I Bsu36I BtsI DraI EaeI EarI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I MmeI MslI
MspA1I NspI PpuMI PshAI PsrI PstI SacI SanDI
SapI ScaI SfcI SmlI SpeI SspI StuI StyI
TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvaI BaeI BamHI
BanI BbeI BciVI BclI BfrBI BglI BlpI BmgBI
BmrI BmtI BplI BpuEI BsaI BsaAI BsaXI BsiWI
BsmI BsmBI BspEI BsrBI BssHII BssSI BstBI BstEII
BtgI BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PvuI PvuII RsrII SacII SalI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SwaI Tth111I XhoI XmaI ZraI