################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BcnI BfaI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI BstBI BstEII BstPI Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI KspI MaeI MfeI MluI MroI MunI NarI NciI NdeI NheI NotI NruI NsiI NspV PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 130 GTATAC GT^[AC][GT]AC AccII 29 CGCG CG^CG AclI 357 AACGTT AA^CGTT AcsI 1260 AAATTC [GA]^AATT[CT] AflIII 1076 ACACGT A^C[GA][CT]GT AhdI 616 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 820 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 1260 AAATTC [GA]^AATT[CT] AseI 880 ATTAAT AT^TAAT AsnI 880 ATTAAT AT^TAAT AspEI 616 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1066 GAGCAC G[AT]GC[AT]^C BalI 533 TGGCCA TGG^CCA BanI 1096 GGCACC G^G[CT][GA]CC BanII 792 GAGCCC G[GA]GC[CT]^C BbvCI 15 GCTGAGG CCTCAGC(-5/-2) BciVI 598 GGATAC GTATCC(6/5) BclI 1106 TGATCA T^GATCA BsaBI 676 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 719 CTCCTC GAGGAG(10/8) BsiHKAI 1066 GAGCAC G[AT]GC[AT]^C BspHI 701 TCATGA T^CATGA BspMI 511 GCAGGT ACCTGC(4/8) BsrDI 234 GCAATG GCAATG(2/0) Bst1107I 130 GTATAC GTA^TAC BstAPI 487 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 29 CGCG CG^CG BstYI 1382 AGATCC [GA]^GATC[CT] BstZ17I 130 GTATAC GTA^TAC CfoI 30 GCGC GCG^C Eam1105I 616 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 1106 TGATCA T^GATCA HhaI 30 GCGC GCG^C HinP1I 30 GCGC G^CGC HpaI 360 GTTAAC GTT^AAC MaeIII 239 GTGAC ^GT[ACGT]AC MamI 676 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1382 AGATCC [GA]^GATC[CT] MluNI 533 TGGCCA TGG^CCA MscI 533 TGGCCA TGG^CCA MvnI 29 CGCG CG^CG NaeI 32 GCCGGC GCC^GGC NgoMIV 32 GCCGGC G^CCGGC NspI 483 ACATGC [GA]CATG^[CT] PacI 877 TTAATTAA TTAAT^TAA PflMI 1282 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 880 ATTAAT AT^TAAT Psp1406I 357 AACGTT AA^CGTT PstI 826 CTGCAG CTGCA^G RcaI 701 TCATGA T^CATGA ScaI 308 AGTACT AGT^ACT SexAI 1245 ACCTGGT A^CC[AT]GGT SfaNI 1423 GATGC GCATC(5/9) SfcI 826 CTGCAG C^T[GA][CT]AG Tsp45I 239 GTGAC ^GT[GC]AC Van91I 1282 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1382 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflII 400 CTTAAG C^TTAAG 1306 CTTAAG AlwI 1383 GATCC GGATC(4/5) 296 GGATC Asp700I 412 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 580 GAAGTGATTC BfrI 400 CTTAAG C^TTAAG 1306 CTTAAG BglI 27 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1426 GCCATAGAGGC BpmI 199 CTCCAG CTGGAG(16/14) 575 CTCCAG BsaI 151 GAGACC GGTCTC(1/5) 1156 GAGACC BsmBI 4 GAGACG CGTCTC(1/5) 1443 GAGACG BsmFI 405 GGGAC GGGAC(10/14) 624 GTCCC BsrFI 32 GCCGGC [GA]^CCGG[CT] 154 ACCGGC BsrI 1092 ACTGG ACTGG(1/-1) 1398 CCAGT BstXI 641 CCATAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 739 CCAGAGCTATGG Cfr10I 32 GCCGGC [GA]^CCGG[CT] 154 ACCGGC DraII 115 GGGTCCT [GA]G^G[ACGT]CC[CT] 1325 AGGACCT EaeI 156 CGGCCA [CT]^GGCC[GA] 533 TGGCCA EcoO109I 115 GGGTCCT [GA]G^G[ACGT]CC[CT] 1325 AGGACCT FauI 24 CCCGC CCCGC(4/6) 927 GCGGG HincII 360 GTTAAC GT[CT]^[GA]AC 515 GTCAAC HindII 360 GTTAAC GT[CT]^[GA]AC 515 GTCAAC HphI 1003 GGTGA GGTGA(8/7) 1104 GGTGA MaeII 358 ACGT A^CGT 1078 ACGT PpuMI 115 GGGTCCT [GA]G^G[AT]CC[CT] 1325 AGGACCT PvuII 13 CAGCTG CAG^CTG 37 CAGCTG SmlI 400 CTTAAG C^T[CT][GA]AG 1306 CTTAAG TaqI 342 TCGA T^CGA 993 TCGA TthHB8I 342 TCGA T^CGA 993 TCGA XmnI 412 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 580 GAAGTGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AfaI AluI AvaII BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EarI EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SspI StyI TfiI Tru9I TseI Tsp509I TspRI