(Circular) (Six-base) MAPSORT of: pFN21AB7746.seq Check: 3776 from: 1 to: 6166 pFN21AB7746 6166 bp With 182 enzymes: SgfI * March 28, 2012 12:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3976 278 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2136 3509 1937 Size: 199 1373 4594 Fragments arranged by size: 4594 1373 199 Acc65I G'GTAC_C Cuts at: 3487 3487 Size: 6166 AclI AA'CG_TT Cuts at: 1563 2363 4293 4666 1563 Size: 800 1930 373 3063 Fragments arranged by size: 3063 1930 800 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2679 3255 3320 4229 5613 5881 1632 Size: 1047 576 65 909 1384 268 1917 Fragments arranged by size: 1917 1384 1047 909 576 268 65 AflII C'TTAA_G Cuts at: 820 1017 2405 3311 820 Size: 197 1388 906 3675 Fragments arranged by size: 3675 1388 906 197 AflIII A'CryG_T Cuts at: 1203 3081 5065 6042 1203 Size: 1878 1984 977 1327 Fragments arranged by size: 1984 1878 1327 977 AgeI A'CCGG_T Cuts at: 4997 4997 Size: 6166 AhdI GACnn_n'nnGTC Cuts at: 2626 4895 2626 Size: 2269 3897 AleI CACnn'nnGTG Cuts at: 1166 3107 1166 Size: 1941 4225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6166 AlwNI CAG_nnn'CTG Cuts at: 2830 3909 5481 2830 Size: 1079 1572 3515 Fragments arranged by size: 3515 1572 1079 ApaLI G'TGCA_C Cuts at: 4223 5379 4223 Size: 1156 5010 ApoI r'AATT_y Cuts at: 1493 3265 3475 3701 3853 5962 1493 Size: 1772 210 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 210 152 AseI AT'TA_AT Cuts at: 160 1032 2886 4720 160 Size: 872 1854 1834 1606 Fragments arranged by size: 1854 1834 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 AvaI C'yCGr_G Cuts at: 1870 1943 3491 1870 Size: 73 1548 4545 Fragments arranged by size: 4545 1548 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2596 2629 2596 Size: 33 6133 BamHI G'GATC_C Cuts at: 3496 3863 3496 Size: 367 5799 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3101 3487 4942 618 Size: 325 217 627 1314 386 1455 1842 Fragments arranged by size: 1842 1455 1314 627 386 325 217 BanII G_rGCy'C Cuts at: 1391 2801 3485 1391 Size: 1410 684 4072 Fragments arranged by size: 4072 1410 684 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5195 BbvCI CC'TCA_GC Cuts at: 2021 2021 Size: 6166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4357 4391 1903 Size: 34 2420 34 3678 Fragments arranged by size: 3678 2420 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2597 4058 5268 2597 Size: 1461 1210 3495 Fragments arranged by size: 3495 1461 1210 BclI T'GATC_A Cuts at: 1548 3111 1548 Size: 1563 4603 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2038 3437 4777 Size: 107 122 71 631 605 366 1399 1340 Cuts at: 4777 136 Size: 1525 Fragments arranged by size: 1525 1399 1340 631 605 366 122 107 71 BglII A'GATC_T Cuts at: 6161 6161 Size: 6166 BlpI GC'TnA_GC Cuts at: 3583 3583 Size: 6166 Bme1580I G_kGCm'C Cuts at: 1790 3023 3104 4227 5383 1790 Size: 1233 81 1123 1156 2573 Fragments arranged by size: 2573 1233 1156 1123 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6166 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3107 3897 4855 Size: 416 53 83 399 24 1690 790 958 Cuts at: 4855 442 Size: 1753 Fragments arranged by size: 1753 1690 958 790 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2565 4826 1239 Size: 950 376 2261 2579 Fragments arranged by size: 2579 2261 950 376 Bpu10I CC'TnA_GC Cuts at: 2021 2802 2021 Size: 781 5385 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4291 5156 5454 4291 Size: 865 298 5003 Fragments arranged by size: 5003 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2150 3155 4829 882 Size: 617 651 1005 1674 2219 Fragments arranged by size: 2219 1674 1005 651 617 BsaAI yAC'GTr Cuts at: 493 1204 3957 493 Size: 711 2753 2702 Fragments arranged by size: 2753 2702 711 BsaBI GATnn'nnATC Cuts at: 2685 6160 2685 Size: 3475 2691 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3973 4355 4990 275 Size: 53 83 186 936 2440 382 635 1451 Fragments arranged by size: 2440 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4599 4997 5271 5418 5829 4599 Size: 398 274 147 411 4936 Fragments arranged by size: 4936 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2707 2737 1178 Size: 30 165 30 1304 30 4607 Fragments arranged by size: 4607 1304 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2715 1186 Size: 1529 4637 BseYI C'CCAG_C Cuts at: 2925 5369 5865 2925 Size: 2444 496 3226 Fragments arranged by size: 3226 2444 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5593 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3616 4377 5405 1540 Size: 275 186 1615 761 1028 2301 Fragments arranged by size: 2301 1615 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3075 4227 4312 5383 3075 Size: 1152 85 1071 3858 Fragments arranged by size: 3858 1152 1071 85 BsmI GAATG_Cn' Cuts at: 3677 3770 3677 Size: 93 6073 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 3442 2003 Size: 1439 4727 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2801 3023 3075 3104 3485 4227 4312 Size: 399 1011 222 52 29 381 742 85 Cuts at: 4312 5383 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1011 742 399 381 222 85 52 29 BspEI T'CCGG_A Cuts at: 5829 5829 Size: 6166 BspHI T'CATG_A Cuts at: 2706 4055 5785 2706 Size: 1349 1730 3087 Fragments arranged by size: 3087 1730 1349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2507 3521 844 Size: 1663 1014 3489 Fragments arranged by size: 3489 1663 1014 BsrBI CCG'CTC Cuts at: 1394 1823 4053 1394 Size: 429 2230 3507 Fragments arranged by size: 3507 2230 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2246 4661 4835 65 Size: 1183 998 2415 174 1396 Fragments arranged by size: 2415 1396 1183 998 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 2159 4810 4997 1700 Size: 110 227 122 2651 187 2869 Fragments arranged by size: 2869 2651 227 187 122 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6166 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6166 BssSI C'ACGA_G Cuts at: 4226 5238 4226 Size: 1012 5154 BstAPI GCAn_nnn'nTGC Cuts at: 794 2498 794 Size: 1704 4462 BstBI TT'CG_AA Cuts at: 4970 4970 Size: 6166 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6166 BstXI CCAn_nnnn'nTGG Cuts at: 2653 2751 2653 Size: 98 6068 BstYI r'GATC_y Cuts at: 1972 3387 3496 3863 4248 4265 5717 5803 5815 Size: 1415 109 367 385 17 1452 86 12 Cuts at: 5815 6161 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1415 385 367 346 109 86 17 12 BstZ17I GTA'TAC Cuts at: 2137 2137 Size: 6166 BtgI C'CryG_G Cuts at: 513 1065 2004 2535 2895 3105 513 Size: 552 939 531 360 210 3574 Fragments arranged by size: 3574 939 552 531 360 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5427 Fragments arranged by size: 5427 683 56 BtsI GCAGTG_nn' Cuts at: 3009 3682 4476 4496 3009 Size: 673 794 20 4679 Fragments arranged by size: 4679 794 673 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6166 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6131 DraI TTT'AAA Cuts at: 3470 3470 Size: 6166 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5173 809 Size: 507 3857 1802 Fragments arranged by size: 3857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2161 2538 3613 Size: 54 1425 50 275 18 331 377 1075 Cuts at: 3613 4502 6131 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1075 889 377 331 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3613 1537 Size: 275 1801 4090 Fragments arranged by size: 4090 1801 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2961 3126 3335 3378 3440 4096 1353 Size: 1608 165 209 43 62 656 3423 Fragments arranged by size: 3423 1608 656 209 165 62 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4753 5127 5273 387 Size: 1064 138 3164 374 146 1280 Fragments arranged by size: 3164 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2565 2679 3255 3320 4229 4826 Size: 393 557 376 114 576 65 909 597 Cuts at: 4826 5613 5881 1239 Size: 787 268 1524 Fragments arranged by size: 1524 909 787 597 576 557 393 376 268 114 65 EcoO109I rG'GnC_Cy Cuts at: 2121 3331 2121 Size: 1210 4956 EcoRI G'AATT_C Cuts at: 3475 3853 3475 Size: 378 5788 EcoRV GAT'ATC Cuts at: 2341 2452 2683 2341 Size: 111 231 5824 Fragments arranged by size: 5824 231 111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3364 3364 Size: 6166 FspI TGC'GCA Cuts at: 4672 4672 Size: 6166 HaeII r_GCGC'y Cuts at: 1678 5313 1678 Size: 3635 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2612 2644 2738 2770 4807 Size: 32 135 32 1040 32 94 32 2037 Cuts at: 4807 4839 4881 4913 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2037 1040 135 94 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2367 2522 3510 3756 669 Size: 988 281 429 155 988 246 3079 Fragments arranged by size: 3079 988 988 429 281 246 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 6166 HpaI GTT'AAC Cuts at: 2367 3756 2367 Size: 1389 4777 KpnI G_GTAC'C Cuts at: 3491 3491 Size: 6166 MfeI C'AATT_G Cuts at: 3765 3765 Size: 6166 MluI A'CGCG_T Cuts at: 6042 6042 Size: 6166 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 3299 5280 5464 1305 Size: 729 1265 1981 184 2007 Fragments arranged by size: 2007 1981 1265 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2540 10 Size: 54 1425 343 708 3636 Fragments arranged by size: 3636 1425 708 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2651 2900 3107 4124 4483 4642 518 Size: 648 1485 249 207 1017 359 159 2042 Fragments arranged by size: 2042 1485 1017 648 359 249 207 159 MspA1I CmG'CkG Cuts at: 1606 2020 2044 2932 3582 4259 5407 5652 1606 Size: 414 24 888 650 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 677 650 414 245 24 NaeI GCC'GGC Cuts at: 2039 2039 Size: 6166 NcoI C'CATG_G Cuts at: 513 1065 2004 2535 2895 3105 513 Size: 552 939 531 360 210 3574 Fragments arranged by size: 3574 939 552 531 360 210 NdeI CA'TA_TG Cuts at: 387 387 Size: 6166 NgoMIV G'CCGG_C Cuts at: 2037 2037 Size: 6166 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6166 NotI GC'GGCC_GC Cuts at: 3613 3613 Size: 6166 NspI r_CATG'y Cuts at: 1132 1229 2492 3524 5069 1132 Size: 97 1263 1032 1545 2229 Fragments arranged by size: 2229 1545 1263 1032 97 PacI TTA_AT'TAA Cuts at: 2886 2886 Size: 6166 PasI CC'CwG_GG Cuts at: 1348 2632 1348 Size: 1284 4882 PciI A'CATG_T Cuts at: 5065 5065 Size: 6166 PflMI CCAn_nnn'nTGG Cuts at: 1108 3293 1108 Size: 2185 3981 PfoI T'CCnGG_A Cuts at: 2205 3423 2205 Size: 1218 4948 PmeI GTTT'AAAC Cuts at: 3470 3470 Size: 6166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4235 4267 5748 5780 4235 Size: 32 1481 32 4621 Fragments arranged by size: 4621 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2121 3331 2121 Size: 1210 4956 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6166 PsiI TTA'TAA Cuts at: 3736 3736 Size: 6166 PstI C_TGCA'G Cuts at: 830 2835 3518 830 Size: 2005 683 3478 Fragments arranged by size: 3478 2005 683 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6166 PvuII CAG'CTG Cuts at: 2020 2044 2020 Size: 24 6142 SalI G'TCGA_C Cuts at: 1936 3508 1936 Size: 1572 4594 SbfI CC_TGCA'GG Cuts at: 3518 3518 Size: 6166 ScaI AGT'ACT Cuts at: 2315 4414 6126 2315 Size: 2099 1712 2355 Fragments arranged by size: 2355 2099 1712 SexAI A'CCwGG_T Cuts at: 3250 3250 Size: 6166 SfcI C'TryA_G Cuts at: 826 1045 2831 3514 4649 5330 5521 826 Size: 219 1786 683 1135 681 191 1471 Fragments arranged by size: 1786 1471 1135 683 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6166 SmaI CCC'GGG Cuts at: 1872 3493 1872 Size: 1621 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 3311 4270 5171 5433 820 Size: 197 926 462 906 959 901 262 1553 Fragments arranged by size: 1553 959 926 906 901 462 262 197 SnaBI TAC'GTA Cuts at: 493 3957 493 Size: 3464 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6166 SphI G_CATG'C Cuts at: 1132 3524 1132 Size: 2392 3774 SspI AAT'ATT Cuts at: 5 52 2267 2391 2467 3197 4090 5 Size: 47 2215 124 76 730 893 2081 Fragments arranged by size: 2215 2081 893 730 124 76 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6166 StyI C'CwwG_G Cuts at: 513 1065 2004 2535 2895 3105 3605 513 Size: 552 939 531 360 210 500 3074 Fragments arranged by size: 3074 939 552 531 500 360 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2853 2996 4193 4210 4363 4548 4993 Size: 1255 1273 143 1197 17 153 185 445 Cuts at: 4993 325 Size: 1498 Fragments arranged by size: 1498 1273 1255 1197 445 185 153 143 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2313 4412 6124 Size: 274 80 33 51 1443 336 2099 1712 Cuts at: 6124 96 Size: 138 Fragments arranged by size: 2099 1712 1443 336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6126 6158 6126 Size: 32 6134 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6166 XbaI T'CTAG_A Cuts at: 3502 3502 Size: 6166 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6166 XmaI C'CCGG_G Cuts at: 1870 3491 1870 Size: 1621 4545 XmnI GAAnn'nnTTC Cuts at: 2421 2589 4295 2421 Size: 168 1706 4292 Fragments arranged by size: 4292 1706 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3974 276 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI