################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII AccI AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KspI MaeIII MamI MfeI MluI MluNI MscI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 845 GGTACC G^GTACC AccIII 455 TCCGGA T^CCGGA AcsI 422 AAATTC [GA]^AATT[CT] Aor51HI 673 AGCGCT AGC^GCT ApoI 422 AAATTC [GA]^AATT[CT] Asp700I 368 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 845 GGTACC G^GTACC BamHI 862 GGATCC G^GATCC BfaI 22 CTAG C^TAG BsaAI 63 CACGTA [CT]AC^GT[GA] BseAI 455 TCCGGA T^CCGGA BsgI 898 CTGCAC GTGCAG(16/14) BsmAI 703 GTCTC GTCTC(1/5) BspEI 455 TCCGGA T^CCGGA BspMI 875 ACCTGC ACCTGC(4/8) BsrBI 90 GAGCGG CCGCTC(-3/-3) BstF5I 743 GGATG GGATG(2/0) DraII 407 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 683 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 884 CCACGG C^C[GA][CT]GG EarI 497 CTCTTC CTCTTC(1/4) Eco47III 673 AGCGCT AGC^GCT EcoO109I 407 AGGACCT [GA]G^G[ACGT]CC[CT] FokI 743 GGATG GGATG(9/13) KpnI 845 GGTACC GGTAC^C Ksp632I 497 CTCTTC CTCTTC(1/4) MaeI 22 CTAG C^TAG MroI 455 TCCGGA T^CCGGA NlaIII 258 CATG CATG^ PpuMI 407 AGGACCT [GA]G^G[AT]CC[CT] PstI 877 CTGCAG CTGCA^G PvuII 487 CAGCTG CAG^CTG SapI 496 GCTCTTC GCTCTTC(1/4) SbfI 876 CCTGCAGG CCTGCA^GG SfaNI 924 GCATC GCATC(5/9) SfcI 877 CTGCAG C^T[GA][CT]AG SmaI 108 CCCGGG CCC^GGG SmlI 412 CTTGAG C^T[CT][GA]AG Sse8387I 876 CCTGCAGG CCTGCA^GG TaqI 40 TCGA T^CGA Tsp509I 423 AATT ^AATT TspRI 768 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 40 TCGA T^CGA XcmI 479 CCAGTTTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 108 CCCGGG C^CCGGG XmaI 108 CCCGGG C^CCGGG XmnI 368 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BanII 240 GGGCTC G[GA]GC[CT]^C 795 GGGCTC BglI 107 GCCCGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 547 GCCGAGCTGGC BmyI 240 GGGCTC G[AGT]GC[ACT]^C 795 GGGCTC BsaWI 168 TCCGGT [AT]^CCGG[AT] 455 TCCGGA Bsp1286I 240 GGGCTC G[AGT]GC[ACT]^C 795 GGGCTC BstYI 644 AGATCC [GA]^GATC[CT] 862 GGATCC MaeII 64 ACGT A^CGT 296 ACGT MboII 624 GAAGA GAAGA(8/7) 498 TCTTC MflI 644 AGATCC [GA]^GATC[CT] 862 GGATCC PshAI 3 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 579 GACTCAGGTC XhoII 644 AGATCC [GA]^GATC[CT] 862 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaI AvaII BanI BbeI BbvI BcnI BpmI BsaHI BsaJI BsiYI BslI BsmFI BsoBI BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MboI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfoI TseI