Restriction map for
pFN21AB7645
(Circular) (Six-base) MAPSORT of: pFN21AB7645.seq Check: 965 from: 1 to: 5689
pFN21AB7645 5689 bp
With 182 enzymes: SgfI *
March 28, 2012 12:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2889 2889
Size: 5689
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 2850 3010 2850
Size: 160 5529
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2489 3752 5136 5404 1632
Size: 857 1263 1384 268 1917
Fragments arranged by size:
1917 1384 1263 857 268
AfeI AGC'GCT
Cuts at: 2680 2680
Size: 5689
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2600 2632 2600
Size: 32 5657
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2427 2998 3224 3376 5485 1493
Size: 934 571 226 152 2109 1697
Fragments arranged by size:
2109 1697 934 571 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2113 2797 2809 2863 3014 1870
Size: 73 149 21 684 12 54 151 4545
Fragments arranged by size:
4545 684 151 149 73 54 21 12
BamHI G'GATC_C
Cuts at: 2867 3019 3386 2867
Size: 152 367 5170
Fragments arranged by size:
5170 367 152
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2786 2850 2893 2960
Size: 325 217 627 318 681 64 43 67
Cuts at: 2960 3010 4465 618
Size: 50 1455 1842
Fragments arranged by size:
1842 1455 681 627 325 318 217 67
64 50 43
BanII G_rGCy'C
Cuts at: 1391 2249 2804 3008 1391
Size: 858 555 204 4072
Fragments arranged by size:
4072 858 555 204
BbeI G_GCGC'C
Cuts at: 2109 2790 2897 2964 2109
Size: 681 107 67 4834
Fragments arranged by size:
4834 681 107 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4718
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2118 2558 4300
Size: 107 122 71 631 605 446 440 1742
Cuts at: 4300 136
Size: 1525
Fragments arranged by size:
1742 1525 631 605 446 440 122 107
71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 3750 4906 1790
Size: 1960 1156 2573
Fragments arranged by size:
2573 1960 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3420 4378 442
Size: 416 53 83 399 24 2003 958 1753
Fragments arranged by size:
2003 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 2047 2467 2788 4349 1239
Size: 792 16 420 321 1561 2579
Fragments arranged by size:
2579 1561 792 420 321 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2438 3814 4679 4977 2438
Size: 1376 865 298 3150
Fragments arranged by size:
3150 1376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2070 3480 493
Size: 711 866 1410 2702
Fragments arranged by size:
2702 1410 866 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 2787 2894 2961
Size: 53 83 186 936 573 681 107 67
Cuts at: 2961 3496 3878 4513 275
Size: 535 382 635 1451
Fragments arranged by size:
1451 936 681 635 573 535 382 186
107 83 67 53
BsaWI w'CCGG_w
Cuts at: 2173 2460 4122 4520 4794 4941 5352 2173
Size: 287 1662 398 274 147 411 2510
Fragments arranged by size:
2510 1662 411 398 287 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5689
BseYI C'CCAG_C
Cuts at: 2386 4892 5388 2386
Size: 2506 496 2687
Fragments arranged by size:
2687 2506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2888 1163
Size: 573 1152 3964
Fragments arranged by size:
3964 1152 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3750 3835 4906 3750
Size: 85 1071 4533
Fragments arranged by size:
4533 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2249 2804 3008 3750 3835 4906 1391
Size: 399 459 555 204 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 555 459 399 204 85
BspEI T'CCGG_A
Cuts at: 2460 5352 2460
Size: 2892 2797
BspHI T'CATG_A
Cuts at: 3578 5308 3578
Size: 1730 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2889 3044 844
Size: 2045 155 3489
Fragments arranged by size:
3489 2045 155
BsrBI CCG'CTC
Cuts at: 1394 1823 2097 3576 1394
Size: 429 274 1479 3507
Fragments arranged by size:
3507 1479 429 274
BsrDI GCAATG_nn'
Cuts at: 65 1248 4184 4358 65
Size: 1183 2936 174 1396
Fragments arranged by size:
2936 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2108 2613 2716 2789 4333 4520 1700
Size: 110 298 505 103 73 1544 187 2869
Fragments arranged by size:
2869 1544 505 298 187 110 103 73
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2649 2867 3019 3386 3771 3788 5240 5326
Size: 677 218 152 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 677 385 367 346 218 152
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2889 513
Size: 552 1824 3313
Fragments arranged by size:
3313 1824 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2779 3205 3999 4019 2779
Size: 426 794 20 4449
Fragments arranged by size:
4449 794 426 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DraIII CAC_nnn'GTG
Cuts at: 2693 2693
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654
Size: 54 1425 50 275 18 1306 889 1629
Cuts at: 5654 8
Size: 43
Fragments arranged by size:
1629 1425 1306 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 3619 1353
Size: 1155 1111 3423
Fragments arranged by size:
3423 1155 1111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2302 2860 4276 4650 4796 387
Size: 1064 138 713 558 1416 374 146 1280
Fragments arranged by size:
1416 1280 1064 713 558 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2047 2467 2489 2788 3752 4349
Size: 393 399 16 420 22 299 964 597
Cuts at: 4349 5136 5404 1239
Size: 787 268 1524
Fragments arranged by size:
1524 964 787 597 420 399 393 299
268 22 16
EcoO109I rG'GnC_Cy
Cuts at: 2413 2413
Size: 5689
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2109 2682 2717 2790 2897 2964 4836 1678
Size: 431 573 35 73 107 67 1872 2531
Fragments arranged by size:
2531 1872 573 431 107 73 67 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 4330 4362 4404
Size: 32 135 32 455 32 2271 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 2271 455 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KasI G'GCGC_C
Cuts at: 2105 2786 2893 2960 2105
Size: 681 107 67 4834
Fragments arranged by size:
4834 681 107 67
KpnI G_GTAC'C
Cuts at: 2854 3014 2854
Size: 160 5529
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4803 4987 1305
Size: 3498 184 2007
Fragments arranged by size:
3498 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3867
Fragments arranged by size:
3867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3647 4006 4165 518
Size: 648 2481 359 159 2042
Fragments arranged by size:
2481 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2289 2494 2509 2746 2784 2899 3105 3782
Size: 683 205 15 237 38 115 206 677
Cuts at: 3782 4930 5175 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 683 677 245 237 206 205
115 38 15
NaeI GCC'GGC
Cuts at: 2110 2718 2791 2110
Size: 608 73 5008
Fragments arranged by size:
5008 608 73
NarI GG'CG_CC
Cuts at: 2106 2787 2894 2961 2106
Size: 681 107 67 4834
Fragments arranged by size:
4834 681 107 67
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NgoMIV G'CCGG_C
Cuts at: 2108 2716 2789 2108
Size: 608 73 5008
Fragments arranged by size:
5008 608 73
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2669 1348
Size: 1321 4368
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2413 2413
Size: 5689
PshAI GACnn'nnGTC
Cuts at: 1613 2012 2588 1613
Size: 399 576 4714
Fragments arranged by size:
4714 576 399
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PstI C_TGCA'G
Cuts at: 830 2886 3041 830
Size: 2056 155 3478
Fragments arranged by size:
3478 2056 155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2494 2494
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SapI GCTCTTCn'nnn_
Cuts at: 2508 2508
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 2886 3041 2886
Size: 155 5534
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2882 3037 4172 4853 5044 826
Size: 219 1837 155 1135 681 191 1471
Fragments arranged by size:
1837 1471 1135 681 219 191 155
SfoI GGC'GCC
Cuts at: 2107 2788 2895 2962 2107
Size: 681 107 67 4834
Fragments arranged by size:
4834 681 107 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2115 3016 1872
Size: 243 901 4545
Fragments arranged by size:
4545 901 243
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2417 3793 4694 4956 820
Size: 197 926 474 1376 901 262 1553
Fragments arranged by size:
1553 1376 926 901 474 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 3128 513
Size: 552 2063 3074
Fragments arranged by size:
3074 2063 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2594 3716 3733 3886 4071 4516 325
Size: 1255 1014 1122 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1122 1014 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3935 5647 96
Size: 274 80 33 51 1443 1958 1712 138
Fragments arranged by size:
1958 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 2629 2661 5649 5681 2252
Size: 32 345 32 2988 32 2260
Fragments arranged by size:
2988 2260 345 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2491 2491
Size: 5689
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 2113 3014 1870
Size: 243 901 4545
Fragments arranged by size:
4545 901 243
XmnI GAAnn'nnTTC
Cuts at: 2377 3818 2377
Size: 1441 4248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SrfI SwaI