################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AviII BbeI BcgI BfrI BsaBI BsaHI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI DraI DrdI Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MluI MroI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RcaI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SnaBI SpeI Sse8387I SspBI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 3299 CGCG CG^CG AgeI 2233 ACCGGT A^CCGGT AhdI 1147 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1147 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 713 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 951 CCTAGG C^CTAGG BamHI 999 GGATCC G^GATCC BclI 1221 TGATCA T^GATCA BglI 2026 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1646 AGATCT A^GATCT BlnI 951 CCTAGG C^CTAGG BsaI 2137 GAGACC GGTCTC(1/5) BsaWI 2233 ACCGGT [AT]^CCGG[AT] BsiEI 2998 CGGCCG CG[GA][CT]^CG BsmBI 1056 CGTCTC CGTCTC(1/5) BsmI 793 GAATGC GAATGC(1/-1) BspMI 918 GCAGGT ACCTGC(4/8) BssHII 3298 GCGCGC G^CGCGC BstUI 3299 CGCG CG^CG BstXI 378 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 3065 CGGACCG CG^G[AT]CCG EagI 2998 CGGCCG C^GGCCG Eam1105I 1147 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2998 CGGCCG C^GGCCG Eco52I 2998 CGGCCG C^GGCCG FbaI 1221 TGATCA T^GATCA MvnI 3299 CGCG CG^CG NaeI 3240 GCCGGC GCC^GGC NgoMIV 3240 GCCGGC G^CCGGC PflFI 713 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2233 ACCGGT A^CCGGT RsrII 3065 CGGACCG CG^G[AT]CCG SgrAI 3239 CGCCGGCG C[GA]^CCGG[CT]G SspI 1265 AATATT AAT^ATT Tth111I 713 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1006 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 504 GTCTAC GT^[AC][GT]AC 3460 GTCTAC Alw44I 2452 GTGCAC G^TGCAC 3166 GTGCAC ApaLI 2452 GTGCAC G^TGCAC 3166 GTGCAC BalI 2038 TGGCCA TGG^CCA 2096 TGGCCA BbvCI 287 CCTCAGC CCTCAGC(-5/-2) 1784 CCTCAGC BciVI 3469 GGATAC GTATCC(6/5) 3405 GTATCC BlpI 1993 GCTCAGC GC^T[ACGT]AGC 3046 GCTCAGC Bpu1102I 1993 GCTCAGC GC^T[ACGT]AGC 3046 GCTCAGC BstEII 282 GGTCACC G^GT[ACGT]ACC 2481 GGTCACC BstPI 282 GGTCACC G^GT[ACGT]ACC 2481 GGTCACC CelII 1993 GCTCAGC GC^T[ACGT]AGC 3046 GCTCAGC EcoO65I 282 GGTCACC G^GT[ACGT]ACC 2481 GGTCACC HaeII 1276 AGCGCC [GA]GCGC^[CT] 3439 AGCGCC HincII 2884 GTCAAC GT[CT]^[GA]AC 3493 GTCAAC HindII 2884 GTCAAC GT[CT]^[GA]AC 3493 GTCAAC MfeI 1142 CAATTG C^AATTG 2540 CAATTG MluNI 2038 TGGCCA TGG^CCA 2096 TGGCCA MscI 2038 TGGCCA TGG^CCA 2096 TGGCCA MunI 1142 CAATTG C^AATTG 2540 CAATTG SacI 1991 GAGCTC GAGCT^C 2964 GAGCTC SmaI 2519 CCCGGG CCC^GGG 3368 CCCGGG SmlI 391 CTCAAG C^T[CT][GA]AG 1979 CTCAAG StuI 1916 AGGCCT AGG^CCT 3095 AGGCCT XmaCI 2519 CCCGGG C^CCGGG 3368 CCCGGG XmaI 2519 CCCGGG C^CCGGG 3368 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII