(Circular) (Six-base) MAPSORT of: pFN21AE3385.seq Check: 9139 from: 1 to: 8218 pFN21AE3385 8218 bp With 182 enzymes: SgfI * March 28, 2012 12:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6028 278 Size: 53 83 186 936 4492 2468 Fragments arranged by size: 4492 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2510 5466 5561 1937 Size: 573 2956 95 4594 Fragments arranged by size: 4594 2956 573 95 Acc65I G'GTAC_C Cuts at: 5539 5539 Size: 8218 AclI AA'CG_TT Cuts at: 1563 6345 6718 1563 Size: 4782 373 3063 Fragments arranged by size: 4782 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2714 2888 3368 3992 4947 6281 7665 7933 Size: 1082 174 480 624 955 1334 1384 268 Cuts at: 7933 1632 Size: 1917 Fragments arranged by size: 1917 1384 1334 1082 955 624 480 268 174 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8021 AflIII A'CryG_T Cuts at: 1203 2035 2820 3153 3471 3622 7117 8094 1203 Size: 832 785 333 318 151 3495 977 1327 Fragments arranged by size: 3495 1327 977 832 785 333 318 151 AgeI A'CCGG_T Cuts at: 4238 7049 4238 Size: 2811 5407 AhdI GACnn_n'nnGTC Cuts at: 3157 6947 3157 Size: 3790 4428 AleI CACnn'nnGTG Cuts at: 1166 3716 4115 1166 Size: 2550 399 5269 Fragments arranged by size: 5269 2550 399 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2231 3848 4242 4761 4776 5202 5316 5340 Size: 1047 1617 394 519 15 426 114 24 Cuts at: 5340 1184 Size: 4062 Fragments arranged by size: 4062 1617 1047 519 426 394 114 24 15 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2690 2722 2690 Size: 32 8186 AlwNI CAG_nnn'CTG Cuts at: 4066 4090 4165 5195 5961 7533 4066 Size: 24 75 1030 766 1572 4751 Fragments arranged by size: 4751 1572 1030 766 75 24 ApaI G_GGCC'C Cuts at: 3112 5012 5372 3112 Size: 1900 360 5958 Fragments arranged by size: 5958 1900 360 ApaLI G'TGCA_C Cuts at: 4457 5171 6275 7431 4457 Size: 714 1104 1156 5244 Fragments arranged by size: 5244 1156 1104 714 ApoI r'AATT_y Cuts at: 1493 5527 5753 5905 8014 1493 Size: 4034 226 152 2109 1697 Fragments arranged by size: 4034 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6772 160 Size: 872 5740 1606 Fragments arranged by size: 5740 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8218 AvaI C'yCGr_G Cuts at: 1870 1943 4139 4524 5373 5401 5543 1870 Size: 73 2196 385 849 28 142 4545 Fragments arranged by size: 4545 2196 849 385 142 73 28 AvrII C'CTAG_G Cuts at: 2956 2956 Size: 8218 BamHI G'GATC_C Cuts at: 3004 5548 5915 3004 Size: 2544 367 5307 Fragments arranged by size: 5307 2544 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2663 4154 4882 5267 5539 Size: 325 217 627 876 1491 728 385 272 Cuts at: 5539 6994 618 Size: 1455 1842 Fragments arranged by size: 1842 1491 1455 876 728 627 385 325 272 217 BanII G_rGCy'C Cuts at: 1391 3112 4000 4973 5012 5261 5372 5489 5537 Size: 1721 888 973 39 249 111 117 48 Cuts at: 5537 1391 Size: 4072 Fragments arranged by size: 4072 1721 973 888 249 117 111 48 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2251 2824 3356 928 Size: 971 352 573 532 5790 Fragments arranged by size: 5790 971 573 532 352 BbvCI CC'TCA_GC Cuts at: 2293 3790 2293 Size: 1497 6721 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6409 6443 1903 Size: 34 4472 34 3678 Fragments arranged by size: 4472 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5421 5468 6110 7320 5421 Size: 47 642 1210 6319 Fragments arranged by size: 6319 1210 642 47 BclI T'GATC_A Cuts at: 1548 3226 1548 Size: 1678 6540 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4037 6829 136 Size: 107 122 71 631 605 2365 2792 1525 Fragments arranged by size: 2792 2365 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3651 8213 3651 Size: 4562 3656 BlpI GC'TnA_GC Cuts at: 3999 5052 5635 3999 Size: 1053 583 6582 Fragments arranged by size: 6582 1053 583 Bme1580I G_kGCm'C Cuts at: 1790 2280 2666 3112 3862 4461 4755 5012 5175 Size: 490 386 446 750 599 294 257 163 Cuts at: 5175 5372 6279 7435 1790 Size: 197 907 1156 2573 Fragments arranged by size: 2573 1156 907 750 599 490 446 386 294 257 197 163 BmgBI CAC'GTC Cuts at: 1318 2508 3061 3625 3732 4507 1318 Size: 1190 553 564 107 775 5029 Fragments arranged by size: 5029 1190 775 564 553 107 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 2139 2906 Size: 416 53 83 399 24 711 11 767 Cuts at: 2906 5088 5949 6907 442 Size: 2182 861 958 1753 Fragments arranged by size: 2182 1753 958 861 767 711 416 399 83 53 24 11 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8218 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3570 4399 3570 Size: 829 7389 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2903 4116 4195 4498 4696 4811 4915 4998 Size: 1664 1213 79 303 198 115 104 83 Cuts at: 4998 6878 1239 Size: 1880 2579 Fragments arranged by size: 2579 1880 1664 1213 303 198 115 104 83 79 Bpu10I CC'TnA_GC Cuts at: 2293 3790 2293 Size: 1497 6721 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2381 3969 6343 7208 7506 2381 Size: 1588 2374 865 298 3093 Fragments arranged by size: 3093 2374 1588 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4136 6881 882 Size: 617 2637 2745 2219 Fragments arranged by size: 2745 2637 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2823 3718 4291 6009 493 Size: 711 1619 895 573 1718 2702 Fragments arranged by size: 2702 1718 1619 895 711 573 BsaBI GATnn'nnATC Cuts at: 8212 8212 Size: 8218 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6025 6407 7042 275 Size: 53 83 186 936 4492 382 635 1451 Fragments arranged by size: 4492 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4238 6651 7049 7323 7470 7881 4238 Size: 2413 398 274 147 411 4575 Fragments arranged by size: 4575 2413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2519 2549 4496 4526 4691 Size: 30 165 30 1116 30 1947 30 165 Cuts at: 4691 4721 4895 4925 4928 4958 1178 Size: 30 174 30 3 30 4438 Fragments arranged by size: 4438 1947 1116 174 165 165 30 30 30 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2426 2462 4312 4375 4625 4628 4699 1186 Size: 1240 36 1850 63 250 3 71 4705 Fragments arranged by size: 4705 1850 1240 250 71 63 36 3 BseYI C'CCAG_C Cuts at: 3725 3816 4032 4056 4075 4158 4565 4748 5156 Size: 91 216 24 19 83 407 183 408 Cuts at: 5156 5217 5332 7421 7917 3725 Size: 61 115 2089 496 4026 Fragments arranged by size: 4026 2089 496 408 407 216 183 115 91 83 61 24 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 2585 4139 4212 4757 4786 5336 Size: 573 720 129 1554 73 545 29 550 Cuts at: 5336 1163 Size: 4045 Fragments arranged by size: 4045 1554 720 573 550 545 129 73 29 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5006 5668 6429 7457 1540 Size: 275 186 3005 662 761 1028 2301 Fragments arranged by size: 3005 2301 1028 761 662 275 186 BsiHKAI G_wGCw'C Cuts at: 2585 2759 3080 4000 4461 4973 5175 6279 6364 Size: 174 321 920 461 512 202 1104 85 Cuts at: 6364 7435 2585 Size: 1071 3368 Fragments arranged by size: 3368 1104 1071 920 512 461 321 202 174 85 BsmI GAATG_Cn' Cuts at: 2804 5729 5822 2804 Size: 2925 93 5200 Fragments arranged by size: 5200 2925 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3067 3067 Size: 8218 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2585 2666 2759 3080 3112 3862 Size: 399 490 305 81 93 321 32 750 Cuts at: 3862 4000 4461 4755 4973 5012 5175 5261 5372 Size: 138 461 294 218 39 163 86 111 Cuts at: 5372 5489 5537 6279 6364 7435 1391 Size: 117 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 750 742 490 461 399 321 305 294 218 163 138 117 111 93 86 85 81 48 39 32 BspEI T'CCGG_A Cuts at: 7881 7881 Size: 8218 BspHI T'CATG_A Cuts at: 6107 7837 6107 Size: 1730 6488 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2914 5573 844 Size: 2070 2659 3489 Fragments arranged by size: 3489 2659 2070 BsrBI CCG'CTC Cuts at: 1394 1823 6105 1394 Size: 429 4282 3507 Fragments arranged by size: 4282 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6713 6887 65 Size: 1183 5465 174 1396 Fragments arranged by size: 5465 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2218 3492 4238 4992 5245 5264 6862 Size: 110 408 1274 746 754 253 19 1598 Cuts at: 6862 7049 1700 Size: 187 2869 Fragments arranged by size: 2869 1598 1274 754 746 408 253 187 110 19 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8218 BssHII G'CGCG_C Cuts at: 1925 5303 1925 Size: 3378 4840 BssSI C'ACGA_G Cuts at: 6278 7290 6278 Size: 1012 7206 BstAPI GCAn_nnn'nTGC Cuts at: 794 2575 4066 5195 794 Size: 1781 1491 1129 3817 Fragments arranged by size: 3817 1781 1491 1129 BstBI TT'CG_AA Cuts at: 7022 7022 Size: 8218 BstEII G'GTnAC_C Cuts at: 1184 2287 4486 1184 Size: 1103 2199 4916 Fragments arranged by size: 4916 2199 1103 BstXI CCAn_nnnn'nTGG Cuts at: 2390 2390 Size: 8218 BstYI r'GATC_y Cuts at: 1972 2688 3004 3651 4011 4062 5548 5915 6300 Size: 716 316 647 360 51 1486 367 385 Cuts at: 6300 6317 7769 7855 7867 8213 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1486 1452 716 647 385 367 360 346 316 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3801 4146 4452 4572 5067 5118 Size: 552 939 1797 345 306 120 495 51 Cuts at: 5118 5161 5271 513 Size: 43 110 3460 Fragments arranged by size: 3460 1797 939 552 495 345 306 120 110 51 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7479 Fragments arranged by size: 7479 683 56 BtsI GCAGTG_nn' Cuts at: 2496 3816 4603 4798 5238 5322 5734 6528 6548 Size: 1320 787 195 440 84 412 794 20 Cuts at: 6548 2496 Size: 4166 Fragments arranged by size: 4166 1320 794 787 440 412 195 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8218 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8183 DraI TTT'AAA Cuts at: 5522 5522 Size: 8218 DraIII CAC_nnn'GTG Cuts at: 2284 5284 5314 2284 Size: 3000 30 5188 Fragments arranged by size: 5188 3000 30 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7225 809 Size: 507 5909 1802 Fragments arranged by size: 5909 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3197 4043 4101 Size: 54 1425 50 275 18 1367 846 58 Cuts at: 4101 5003 5665 6554 8183 8 Size: 902 662 889 1629 43 Fragments arranged by size: 1629 1425 1367 902 889 846 662 275 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5003 5665 1537 Size: 275 3191 662 4090 Fragments arranged by size: 4090 3191 662 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4011 4325 4966 6148 1353 Size: 2658 314 641 1182 3423 Fragments arranged by size: 3423 2658 1182 641 314 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2346 4184 6805 7179 7325 387 Size: 1064 138 757 1838 2621 374 146 1280 Fragments arranged by size: 2621 1838 1280 1064 757 374 146 138 EcoICRI GAG'CTC Cuts at: 3998 4971 3998 Size: 973 7245 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2714 2888 2903 3368 3992 4116 4195 Size: 393 1082 174 15 465 624 124 79 Cuts at: 4195 4498 4696 4811 4915 4947 4998 6281 6878 Size: 303 198 115 104 32 51 1283 597 Cuts at: 6878 7665 7933 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1283 1082 787 624 597 465 393 303 268 198 174 124 115 104 79 51 32 15 EcoO109I rG'GnC_Cy Cuts at: 3108 3109 4053 5009 5353 5369 5377 3108 Size: 1 944 956 344 16 8 5949 Fragments arranged by size: 5949 956 944 344 16 8 1 EcoRI G'AATT_C Cuts at: 5527 5905 5527 Size: 378 7840 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2256 2322 2256 Size: 66 8152 FspI TGC'GCA Cuts at: 6724 6724 Size: 8218 HaeII r_GCGC'y Cuts at: 1678 3285 5448 7365 1678 Size: 1607 2163 1917 2531 Fragments arranged by size: 2531 2163 1917 1607 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3018 3050 3143 3175 3308 Size: 32 135 32 1446 32 93 32 133 Cuts at: 3308 3340 4496 4528 5395 5427 6859 6891 6933 Size: 32 1156 32 867 32 1432 32 42 Cuts at: 6933 6965 1373 Size: 32 2626 Fragments arranged by size: 2626 1446 1432 1156 867 135 133 93 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4891 5500 5562 5808 669 Size: 988 281 2953 609 62 246 3079 Fragments arranged by size: 3079 2953 988 609 281 246 62 HindIII A'AGCT_T Cuts at: 748 748 Size: 8218 HpaI GTT'AAC Cuts at: 5808 5808 Size: 8218 KpnI G_GTAC'C Cuts at: 5543 5543 Size: 8218 MfeI C'AATT_G Cuts at: 3147 4545 5817 3147 Size: 1398 1272 5548 Fragments arranged by size: 5548 1398 1272 MluI A'CGCG_T Cuts at: 8094 8094 Size: 8218 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2139 2562 2823 4039 4728 7332 7516 1305 Size: 834 423 261 1216 689 2604 184 2007 Fragments arranged by size: 2604 2007 1216 834 689 423 261 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4045 4103 10 Size: 54 1425 343 2213 58 4125 Fragments arranged by size: 4125 2213 1425 343 58 54 MslI CAynn'nnrTG Cuts at: 518 1166 2999 3716 4115 4758 4826 6176 6535 Size: 648 1833 717 399 643 68 1350 359 Cuts at: 6535 6694 518 Size: 159 2042 Fragments arranged by size: 2042 1833 1350 717 648 643 399 359 159 68 MspA1I CmG'CkG Cuts at: 1606 2532 2560 3766 3794 3820 4165 4446 4537 Size: 926 28 1206 28 26 345 281 91 Cuts at: 4537 4674 4953 5002 5195 5201 5634 6311 7459 Size: 137 279 49 193 6 433 677 1148 Cuts at: 7459 7704 1606 Size: 245 2120 Fragments arranged by size: 2120 1206 1148 926 677 433 345 281 279 245 193 137 91 49 28 28 26 6 NaeI GCC'GGC Cuts at: 5247 5247 Size: 8218 NcoI C'CATG_G Cuts at: 513 1065 2004 3801 4146 4452 5118 513 Size: 552 939 1797 345 306 666 3613 Fragments arranged by size: 3613 1797 939 666 552 345 306 NdeI CA'TA_TG Cuts at: 387 387 Size: 8218 NgoMIV G'CCGG_C Cuts at: 5245 5245 Size: 8218 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8218 NotI GC'GGCC_GC Cuts at: 5665 5665 Size: 8218 NspI r_CATG'y Cuts at: 1132 1229 2039 3157 3475 3829 4127 4190 5576 Size: 97 810 1118 318 354 298 63 1386 Cuts at: 5576 7121 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1386 1118 810 354 318 298 97 63 PasI CC'CwG_GG Cuts at: 1348 3163 5254 1348 Size: 1815 2091 4312 Fragments arranged by size: 4312 2091 1815 PciI A'CATG_T Cuts at: 2035 3153 3471 7117 2035 Size: 1118 318 3646 3136 Fragments arranged by size: 3646 3136 1118 318 PflMI CCAn_nnn'nTGG Cuts at: 1108 3646 4165 5284 1108 Size: 2538 519 1119 4042 Fragments arranged by size: 4042 2538 1119 519 PfoI T'CCnGG_A Cuts at: 3208 4325 3208 Size: 1117 7101 PmeI GTTT'AAAC Cuts at: 5522 5522 Size: 8218 PmlI CAC'GTG Cuts at: 2823 3718 4291 2823 Size: 895 573 6750 Fragments arranged by size: 6750 895 573 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6287 6319 7800 7832 6287 Size: 32 1481 32 6673 Fragments arranged by size: 6673 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4053 5353 5377 4053 Size: 1300 24 6894 Fragments arranged by size: 6894 1300 24 PshAI GACnn'nnGTC Cuts at: 1613 2255 3773 4547 1613 Size: 642 1518 774 5284 Fragments arranged by size: 5284 1518 774 642 PsiI TTA'TAA Cuts at: 4206 5788 4206 Size: 1582 6636 PspOMI G'GGCC_C Cuts at: 3108 5008 5368 3108 Size: 1900 360 5958 Fragments arranged by size: 5958 1900 360 PstI C_TGCA'G Cuts at: 830 3811 3837 4478 5191 5570 830 Size: 2981 26 641 713 379 3478 Fragments arranged by size: 3478 2981 713 641 379 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8218 PvuII CAG'CTG Cuts at: 2560 3766 4165 4674 2560 Size: 1206 399 509 6104 Fragments arranged by size: 6104 1206 509 399 RsrII CG'GwC_CG Cuts at: 5071 5071 Size: 8218 SacI G_AGCT'C Cuts at: 4000 4973 4000 Size: 973 7245 SalI G'TCGA_C Cuts at: 1936 5560 1936 Size: 3624 4594 SanDI GG'GwC_CC Cuts at: 4053 4053 Size: 8218 SbfI CC_TGCA'GG Cuts at: 5570 5570 Size: 8218 ScaI AGT'ACT Cuts at: 6466 8178 6466 Size: 1712 6506 SexAI A'CCwGG_T Cuts at: 4018 5280 5349 4018 Size: 1262 69 6887 Fragments arranged by size: 6887 1262 69 SfcI C'TryA_G Cuts at: 826 1045 2080 3807 3833 4474 5187 5566 6701 Size: 219 1035 1727 26 641 713 379 1135 Cuts at: 6701 7382 7573 826 Size: 681 191 1471 Fragments arranged by size: 1727 1471 1135 1035 713 681 641 379 219 191 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8218 SgrAI Cr'CCGG_yG Cuts at: 1700 5245 1700 Size: 3545 4673 SmaI CCC'GGG Cuts at: 1872 4526 5375 5545 1872 Size: 2654 849 170 4545 Fragments arranged by size: 4545 2654 849 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 2396 3984 6322 7223 7485 820 Size: 197 926 453 1588 2338 901 262 1553 Fragments arranged by size: 2338 1588 1553 926 901 453 262 197 SnaBI TAC'GTA Cuts at: 493 6009 493 Size: 5516 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8218 SphI G_CATG'C Cuts at: 1132 3829 4127 4190 5576 1132 Size: 2697 298 63 1386 3774 Fragments arranged by size: 3774 2697 1386 298 63 SspI AAT'ATT Cuts at: 5 52 3272 6142 5 Size: 47 3220 2870 2081 Fragments arranged by size: 3220 2870 2081 47 StuI AGG'CCT Cuts at: 1517 3923 5102 1517 Size: 2406 1179 4633 Fragments arranged by size: 4633 2406 1179 StyI C'CwwG_G Cuts at: 513 1065 2004 2956 3066 3124 3801 3910 4146 Size: 552 939 952 110 58 677 109 236 Cuts at: 4146 4452 4779 5118 5493 5657 513 Size: 306 327 339 375 164 3074 Fragments arranged by size: 3074 952 939 677 552 375 339 327 306 236 164 110 109 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2473 2718 4121 4172 4268 4474 4485 Size: 1255 893 245 1403 51 96 206 11 Cuts at: 4485 5231 6245 6262 6415 6600 7045 325 Size: 746 1014 17 153 185 445 1498 Fragments arranged by size: 1498 1403 1255 1014 893 746 445 245 206 185 153 96 51 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3405 6464 8176 Size: 274 80 33 51 1443 1428 3059 1712 Cuts at: 8176 96 Size: 138 Fragments arranged by size: 3059 1712 1443 1428 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4120 4152 4892 4895 4924 4927 8178 8210 4120 Size: 32 740 3 29 3 3251 32 4128 Fragments arranged by size: 4128 3251 740 32 32 29 3 3 Tth111I GACn'n_nGTC Cuts at: 1316 2721 1316 Size: 1405 6813 XbaI T'CTAG_A Cuts at: 3011 5554 3011 Size: 2543 5675 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3336 3808 4786 4858 5269 3336 Size: 472 978 72 411 6285 Fragments arranged by size: 6285 978 472 411 72 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8218 XmaI C'CCGG_G Cuts at: 1870 4524 5373 5543 1870 Size: 2654 849 170 4545 Fragments arranged by size: 4545 2654 849 170 XmnI GAAnn'nnTTC Cuts at: 6347 6347 Size: 8218 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6026 276 Size: 53 83 186 936 4492 2468 Fragments arranged by size: 4492 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI SacII SapI SfiI SfoI SrfI SwaI