Restriction map for
pFN21AE3385
(Linear) (Six-base) MAPSORT of: KIEE3385.seq Check: 8757 from: 1 to: 3513
KIEE3385.orf
With 182 enzymes: SgfI *
March 22, 2012 16:31 ..
AccI GT'mk_AC
Cuts at: 0 505 3461 3513
Size: 505 2956 52
Fragments arranged by size:
2956 505 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 883 1363 1987 2942 3513
Size: 709 174 480 624 955 571
Fragments arranged by size:
955 709 624 571 480 174
AflIII A'CryG_T
Cuts at: 0 30 815 1148 1466 1617 3513
Size: 30 785 333 318 151 1896
Fragments arranged by size:
1896 785 333 318 151 30
AgeI A'CCGG_T
Cuts at: 0 2233 3513
Size: 2233 1280
AhdI GACnn_n'nnGTC
Cuts at: 0 1152 3513
Size: 1152 2361
AleI CACnn'nnGTG
Cuts at: 0 1711 2110 3513
Size: 1711 399 1403
Fragments arranged by size:
1711 1403 399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 226 1843 2237 2756 2771 3197 3311 3335
Size: 226 1617 394 519 15 426 114 24
Cuts at: 3335 3513
Size: 178
Fragments arranged by size:
1617 519 426 394 226 178 114 24
15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 685 717 3513
Size: 685 32 2796
Fragments arranged by size:
2796 685 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2061 2085 2160 3190 3513
Size: 2061 24 75 1030 323
Fragments arranged by size:
2061 1030 323 75 24
ApaI G_GGCC'C
Cuts at: 0 1107 3007 3367 3513
Size: 1107 1900 360 146
Fragments arranged by size:
1900 1107 360 146
ApaLI G'TGCA_C
Cuts at: 0 2452 3166 3513
Size: 2452 714 347
Fragments arranged by size:
2452 714 347
AvaI C'yCGr_G
Cuts at: 0 2134 2519 3368 3396 3513
Size: 2134 385 849 28 117
Fragments arranged by size:
2134 849 385 117 28
AvrII C'CTAG_G
Cuts at: 0 951 3513
Size: 951 2562
BamHI G'GATC_C
Cuts at: 0 999 3513
Size: 999 2514
BanI G'GyrC_C
Cuts at: 0 658 2149 2877 3262 3513
Size: 658 1491 728 385 251
Fragments arranged by size:
1491 728 658 385 251
BanII G_rGCy'C
Cuts at: 0 1107 1995 2968 3007 3256 3367 3484 3513
Size: 1107 888 973 39 249 111 117 29
Fragments arranged by size:
1107 973 888 249 117 111 39 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 246 819 1351 3513
Size: 246 573 532 2162
Fragments arranged by size:
2162 573 532 246
BbvCI CC'TCA_GC
Cuts at: 0 288 1785 3513
Size: 288 1497 1728
Fragments arranged by size:
1728 1497 288
BciVI GTATCCnnnnn_n'
Cuts at: 0 3416 3463 3513
Size: 3416 47 50
Fragments arranged by size:
3416 50 47
BclI T'GATC_A
Cuts at: 0 1221 3513
Size: 1221 2292
BglI GCCn_nnn'nGGC
Cuts at: 0 2032 3513
Size: 2032 1481
BglII A'GATC_T
Cuts at: 0 1646 3513
Size: 1646 1867
BlpI GC'TnA_GC
Cuts at: 0 1994 3047 3513
Size: 1994 1053 466
Fragments arranged by size:
1994 1053 466
Bme1580I G_kGCm'C
Cuts at: 0 275 661 1107 1857 2456 2750 3007 3170
Size: 275 386 446 750 599 294 257 163
Cuts at: 3170 3367 3513
Size: 197 146
Fragments arranged by size:
750 599 446 386 294 275 257 197
163 146
BmgBI CAC'GTC
Cuts at: 0 503 1056 1620 1727 2502 3513
Size: 503 553 564 107 775 1011
Fragments arranged by size:
1011 775 564 553 503 107
BmrI ACTGGGnnnn_n'
Cuts at: 0 123 134 901 3083 3513
Size: 123 11 767 2182 430
Fragments arranged by size:
2182 767 430 123 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1565 2394 3513
Size: 1565 829 1119
Fragments arranged by size:
1565 1119 829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 2111 2190 2493 2691 2806 2910 2993
Size: 898 1213 79 303 198 115 104 83
Cuts at: 2993 3513
Size: 520
Fragments arranged by size:
1213 898 520 303 198 115 104 83
79
Bpu10I CC'TnA_GC
Cuts at: 0 288 1785 3513
Size: 288 1497 1728
Fragments arranged by size:
1728 1497 288
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1964 3513
Size: 376 1588 1549
Fragments arranged by size:
1588 1549 376
BsaI GGTCTCn'nnnn_
Cuts at: 0 2131 3513
Size: 2131 1382
BsaAI yAC'GTr
Cuts at: 0 818 1713 2286 3513
Size: 818 895 573 1227
Fragments arranged by size:
1227 895 818 573
BsaWI w'CCGG_w
Cuts at: 0 2233 3513
Size: 2233 1280
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 2491 2521 2686 2716 2890 2920
Size: 514 30 1947 30 165 30 174 30
Cuts at: 2920 2923 2953 3513
Size: 3 30 560
Fragments arranged by size:
1947 560 514 174 165 30 30 30
30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 421 457 2307 2370 2620 2623 2694 3513
Size: 421 36 1850 63 250 3 71 819
Fragments arranged by size:
1850 819 421 250 71 63 36 3
BseYI C'CCAG_C
Cuts at: 0 1720 1811 2027 2051 2070 2153 2560 2743
Size: 1720 91 216 24 19 83 407 183
Cuts at: 2743 3151 3212 3327 3513
Size: 408 61 115 186
Fragments arranged by size:
1720 408 407 216 186 183 115 91
83 61 24 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 580 2134 2207 2752 2781 3331 3513
Size: 451 129 1554 73 545 29 550 182
Fragments arranged by size:
1554 550 545 451 182 129 73 29
BsiEI CG_ry'CG
Cuts at: 0 3001 3513
Size: 3001 512
BsiHKAI G_wGCw'C
Cuts at: 0 580 754 1075 1995 2456 2968 3170 3513
Size: 580 174 321 920 461 512 202 343
Fragments arranged by size:
920 580 512 461 343 321 202 174
BsmI GAATG_Cn'
Cuts at: 0 799 3513
Size: 799 2714
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1062 3513
Size: 1062 2451
Bsp1286I G_dGCh'C
Cuts at: 0 275 580 661 754 1075 1107 1857 1995
Size: 275 305 81 93 321 32 750 138
Cuts at: 1995 2456 2750 2968 3007 3170 3256 3367 3484
Size: 461 294 218 39 163 86 111 117
Cuts at: 3484 3513
Size: 29
Fragments arranged by size:
750 461 321 305 294 275 218 163
138 117 111 93 86 81 39 32
29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 909 3513
Size: 909 2604
BsrFI r'CCGG_y
Cuts at: 0 213 1487 2233 2987 3240 3259 3513
Size: 213 1274 746 754 253 19 254
Fragments arranged by size:
1274 754 746 254 253 213 19
BssHII G'CGCG_C
Cuts at: 0 3298 3513
Size: 3298 215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 570 2061 3190 3513
Size: 570 1491 1129 323
Fragments arranged by size:
1491 1129 570 323
BstEII G'GTnAC_C
Cuts at: 0 282 2481 3513
Size: 282 2199 1032
Fragments arranged by size:
2199 1032 282
BstXI CCAn_nnnn'nTGG
Cuts at: 0 385 3513
Size: 385 3128
BstYI r'GATC_y
Cuts at: 0 683 999 1646 2006 2057 3513
Size: 683 316 647 360 51 1456
Fragments arranged by size:
1456 683 647 360 316 51
BtgI C'CryG_G
Cuts at: 0 1796 2141 2447 2567 3062 3113 3156 3266
Size: 1796 345 306 120 495 51 43 110
Cuts at: 3266 3513
Size: 247
Fragments arranged by size:
1796 495 345 306 247 120 110 51
43
BtsI GCAGTG_nn'
Cuts at: 0 491 1811 2598 2793 3233 3317 3513
Size: 491 1320 787 195 440 84 196
Fragments arranged by size:
1320 787 491 440 196 195 84
DraIII CAC_nnn'GTG
Cuts at: 0 279 3279 3309 3513
Size: 279 3000 30 204
Fragments arranged by size:
3000 279 204 30
EaeI y'GGCC_r
Cuts at: 0 1192 2038 2096 2998 3513
Size: 1192 846 58 902 515
Fragments arranged by size:
1192 902 846 515 58
EagI C'GGCC_G
Cuts at: 0 2998 3513
Size: 2998 515
EarI CTCTTCn'nnn_
Cuts at: 0 2006 2320 2961 3513
Size: 2006 314 641 552
Fragments arranged by size:
2006 641 552 314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 341 2179 3513
Size: 341 1838 1334
Fragments arranged by size:
1838 1334 341
EcoICRI GAG'CTC
Cuts at: 0 1993 2966 3513
Size: 1993 973 547
Fragments arranged by size:
1993 973 547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 883 898 1363 1987 2111 2190 2493
Size: 709 174 15 465 624 124 79 303
Cuts at: 2493 2691 2806 2910 2942 2993 3513
Size: 198 115 104 32 51 520
Fragments arranged by size:
709 624 520 465 303 198 174 124
115 104 79 51 32 15
EcoO109I rG'GnC_Cy
Cuts at: 0 1103 1104 2048 3004 3348 3364 3372 3513
Size: 1103 1 944 956 344 16 8 141
Fragments arranged by size:
1103 956 944 344 141 16 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 251 317 3513
Size: 251 66 3196
Fragments arranged by size:
3196 251 66
HaeII r_GCGC'y
Cuts at: 0 1280 3443 3513
Size: 1280 2163 70
Fragments arranged by size:
2163 1280 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 1138 1170 1303 1335 2491 2523
Size: 1013 32 93 32 133 32 1156 32
Cuts at: 2523 3390 3422 3513
Size: 867 32 91
Fragments arranged by size:
1156 1013 867 133 93 91 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2886 3495 3513
Size: 2886 609 18
Fragments arranged by size:
2886 609 18
MfeI C'AATT_G
Cuts at: 0 1142 2540 3513
Size: 1142 1398 973
Fragments arranged by size:
1398 1142 973
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 557 818 2034 2723 3513
Size: 134 423 261 1216 689 790
Fragments arranged by size:
1216 790 689 423 261 134
MscI TGG'CCA
Cuts at: 0 2040 2098 3513
Size: 2040 58 1415
Fragments arranged by size:
2040 1415 58
MslI CAynn'nnrTG
Cuts at: 0 994 1711 2110 2753 2821 3513
Size: 994 717 399 643 68 692
Fragments arranged by size:
994 717 692 643 399 68
MspA1I CmG'CkG
Cuts at: 0 527 555 1761 1789 1815 2160 2441 2532
Size: 527 28 1206 28 26 345 281 91
Cuts at: 2532 2669 2948 2997 3190 3196 3513
Size: 137 279 49 193 6 317
Fragments arranged by size:
1206 527 345 317 281 279 193 137
91 49 28 28 26 6
NaeI GCC'GGC
Cuts at: 0 3242 3513
Size: 3242 271
NcoI C'CATG_G
Cuts at: 0 1796 2141 2447 3113 3513
Size: 1796 345 306 666 400
Fragments arranged by size:
1796 666 400 345 306
NgoMIV G'CCGG_C
Cuts at: 0 3240 3513
Size: 3240 273
NspI r_CATG'y
Cuts at: 0 34 1152 1470 1824 2122 2185 3513
Size: 34 1118 318 354 298 63 1328
Fragments arranged by size:
1328 1118 354 318 298 63 34
PasI CC'CwG_GG
Cuts at: 0 1158 3249 3513
Size: 1158 2091 264
Fragments arranged by size:
2091 1158 264
PciI A'CATG_T
Cuts at: 0 30 1148 1466 3513
Size: 30 1118 318 2047
Fragments arranged by size:
2047 1118 318 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 1641 2160 3279 3513
Size: 1641 519 1119 234
Fragments arranged by size:
1641 1119 519 234
PfoI T'CCnGG_A
Cuts at: 0 1203 2320 3513
Size: 1203 1117 1193
Fragments arranged by size:
1203 1193 1117
PmlI CAC'GTG
Cuts at: 0 818 1713 2286 3513
Size: 818 895 573 1227
Fragments arranged by size:
1227 895 818 573
PpuMI rG'GwC_Cy
Cuts at: 0 2048 3348 3372 3513
Size: 2048 1300 24 141
Fragments arranged by size:
2048 1300 141 24
PshAI GACnn'nnGTC
Cuts at: 0 250 1768 2542 3513
Size: 250 1518 774 971
Fragments arranged by size:
1518 971 774 250
PsiI TTA'TAA
Cuts at: 0 2201 3513
Size: 2201 1312
PspOMI G'GGCC_C
Cuts at: 0 1103 3003 3363 3513
Size: 1103 1900 360 150
Fragments arranged by size:
1900 1103 360 150
PstI C_TGCA'G
Cuts at: 0 1806 1832 2473 3186 3513
Size: 1806 26 641 713 327
Fragments arranged by size:
1806 713 641 327 26
PvuII CAG'CTG
Cuts at: 0 555 1761 2160 2669 3513
Size: 555 1206 399 509 844
Fragments arranged by size:
1206 844 555 509 399
RsrII CG'GwC_CG
Cuts at: 0 3066 3513
Size: 3066 447
SacI G_AGCT'C
Cuts at: 0 1995 2968 3513
Size: 1995 973 545
Fragments arranged by size:
1995 973 545
SanDI GG'GwC_CC
Cuts at: 0 2048 3513
Size: 2048 1465
SexAI A'CCwGG_T
Cuts at: 0 2013 3275 3344 3513
Size: 2013 1262 69 169
Fragments arranged by size:
2013 1262 169 69
SfcI C'TryA_G
Cuts at: 0 75 1802 1828 2469 3182 3513
Size: 75 1727 26 641 713 331
Fragments arranged by size:
1727 713 641 331 75 26
SgrAI Cr'CCGG_yG
Cuts at: 0 3240 3513
Size: 3240 273
SmaI CCC'GGG
Cuts at: 0 2521 3370 3513
Size: 2521 849 143
Fragments arranged by size:
2521 849 143
SmlI C'TyrA_G
Cuts at: 0 391 1979 3513
Size: 391 1588 1534
Fragments arranged by size:
1588 1534 391
SphI G_CATG'C
Cuts at: 0 1824 2122 2185 3513
Size: 1824 298 63 1328
Fragments arranged by size:
1824 1328 298 63
SspI AAT'ATT
Cuts at: 0 1267 3513
Size: 1267 2246
StuI AGG'CCT
Cuts at: 0 1918 3097 3513
Size: 1918 1179 416
Fragments arranged by size:
1918 1179 416
StyI C'CwwG_G
Cuts at: 0 951 1061 1119 1796 1905 2141 2447 2774
Size: 951 110 58 677 109 236 306 327
Cuts at: 2774 3113 3488 3513
Size: 339 375 25
Fragments arranged by size:
951 677 375 339 327 306 236 110
109 58 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 468 713 2116 2167 2263 2469 2480 3226
Size: 468 245 1403 51 96 206 11 746
Cuts at: 3226 3513
Size: 287
Fragments arranged by size:
1403 746 468 287 245 206 96 51
11
TatI w'GTAC_w
Cuts at: 0 1400 3513
Size: 1400 2113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2115 2147 2887 2890 2919 2922 3513
Size: 2115 32 740 3 29 3 591
Fragments arranged by size:
2115 740 591 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 0 716 3513
Size: 716 2797
XbaI T'CTAG_A
Cuts at: 0 1006 3513
Size: 1006 2507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1331 1803 2781 2853 3264 3513
Size: 1331 472 978 72 411 249
Fragments arranged by size:
1331 978 472 411 249 72
XmaI C'CCGG_G
Cuts at: 0 2519 3368 3513
Size: 2519 849 145
Fragments arranged by size:
2519 849 145
Enzymes that do cut:
AccI AcuI AflIII AgeI AhdI AleI AlfI AloI
AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrFI BssHII BstAPI BstEII BstXI
BstYI BtgI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII MfeI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PstI PvuII RsrII SacI SanDI SexAI SfcI
SgrAI SmaI SmlI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII ApoI AscI
AseI AsiSI BaeI BbeI BcgI BfrBI BmtI BsaBI
BsaHI BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MluI NarI NdeI NheI NotI NruI
NsiI PacI PmeI PpiI PspXI PsrI PvuI SacII
SalI SapI SbfI ScaI SfiI SfoI SgfI SnaBI
SpeI SrfI SwaI XhoI XmnI ZraI