Restriction map for
pFN21AE3336
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbrPI BciVI BclI BfrI BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI CpoI DraI Eam1105I
EcoO65I EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NruI NsiI PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I XbaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 100 GAATTC [GA]^AATT[CT]
AcyI 2114 GGCGCC G[GA]^CG[CT]C
AflIII 381 ACATGT A^C[GA][CT]GT
Alw44I 243 GTGCAC G^TGCAC
Aor51HI 365 AGCGCT AGC^GCT
ApaLI 243 GTGCAC G^TGCAC
ApoI 100 GAATTC [GA]^AATT[CT]
AspI 634 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2114 GGCGCC GGCGC^C
BcgI 1646 GCAAGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2017 CTAG C^TAG
BglI 999 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2570 AGATCT A^GATCT
BlpI 2880 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2880 GCTCAGC GC^T[ACGT]AGC
BsaHI 2114 GGCGCC G[GA]^CG[CT]C
BsmBI 949 CGTCTC CGTCTC(1/5)
BsmI 138 GAATGC GAATGC(1/-1)
BspLU11I 381 ACATGT A^CATGT
BsrDI 1303 CATTGC GCAATG(2/0)
BstBI 59 TTCGAA TT^CGAA
CelII 2880 GCTCAGC GC^T[ACGT]AGC
DraIII 1357 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 365 AGCGCT AGC^GCT
EcoRI 100 GAATTC G^AATTC
FseI 2584 GGCCGGCC GGCCGG^CC
Hin1I 2114 GGCGCC G[GA]^CG[CT]C
KasI 2114 GGCGCC G^GCGCC
MaeI 2017 CTAG C^TAG
NarI 2114 GGCGCC GG^CGCC
NcoI 200 CCATGG C^CATGG
NotI 2865 GCGGCCGC GC^GGCCGC
NspV 59 TTCGAA TT^CGAA
PflFI 634 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 2883 CAGCTG CAG^CTG
SfoI 2114 GGCGCC GGC^GCC
SfuI 59 TTCGAA TT^CGAA
StuI 1520 AGGCCT AGG^CCT
Tsp509I 101 AATT ^AATT
Tth111I 634 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbsI 299 GAAGAC GAAGAC(2/6)
340 GAAGAC
BbvCI 618 CCTCAGC CCTCAGC(-5/-2)
1884 GCTGAGG
BpuAI 299 GAAGAC GAAGAC(2/6)
340 GAAGAC
BsaI 196 GAGACC GGTCTC(1/5)
1117 GAGACC
BsrFI 2585 GCCGGC [GA]^CCGG[CT]
2874 GCCGGC
BstYI 332 AGATCC [GA]^GATC[CT]
2570 AGATCT
Cfr10I 2585 GCCGGC [GA]^CCGG[CT]
2874 GCCGGC
HgaI 948 GCGTC GACGC(5/10)
1269 GCGTC
KspI 1015 CCGCGG CCGC^GG
2104 CCGCGG
MaeII 638 ACGT A^CGT
1748 ACGT
MflI 332 AGATCC [GA]^GATC[CT]
2570 AGATCT
NaeI 2585 GCCGGC GCC^GGC
2874 GCCGGC
NgoMIV 2585 GCCGGC G^CCGGC
2874 GCCGGC
NspI 381 ACATGT [GA]CATG^[CT]
2357 ACATGC
PaeR7I 1330 CTCGAG C^TCGAG
1524 CTCGAG
PshAI 378 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1872 GACTCAGGTC
PstI 1278 CTGCAG CTGCA^G
1823 CTGCAG
SacII 1015 CCGCGG CCGC^GG
2104 CCGCGG
SexAI 722 ACCAGGT A^CC[AT]GGT
1358 ACCTGGT
XhoI 1330 CTCGAG C^TCGAG
1524 CTCGAG
XhoII 332 AGATCC [GA]^GATC[CT]
2570 AGATCT
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AviII BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI