################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII AccI AclI AcsI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbvCI BcgI BclI BfrI BlnI BlpI Bpu1102I BsaHI BsiEI BsiWI BsmBI BspDI BsrBI BssSI Bst1107I BstZ17I Bsu36I CelII ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI FauI FbaI FseI FspI HgaI Hin1I HpaI KasI KspI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 2227 GGTACC G^GTACC AccIII 116 TCCGGA T^CCGGA Alw44I 324 GTGCAC G^TGCAC ApaLI 324 GTGCAC G^TGCAC Asp700I 2563 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2227 GGTACC G^GTACC BanII 129 GAGCCC G[GA]GC[CT]^C BbrPI 2534 CACGTG CAC^GTG BbsI 1638 GAAGAC GAAGAC(2/6) BglI 101 GCCATGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 762 AGATCT A^GATCT BpuAI 1638 GAAGAC GAAGAC(2/6) BsaI 2957 GAGACC GGTCTC(1/5) BsaWI 116 TCCGGA [AT]^CCGG[AT] BseAI 116 TCCGGA T^CCGGA BspEI 116 TCCGGA T^CCGGA BsrFI 226 ACCGGC [GA]^CCGG[CT] BssHII 218 GCGCGC G^CGCGC BstBI 2303 TTCGAA TT^CGAA BstYI 762 AGATCT [GA]^GATC[CT] Cfr10I 226 ACCGGC [GA]^CCGG[CT] DraI 1140 TTTAAA TTT^AAA DraIII 327 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1864 CCATGG C^C[GA][CT]GG EcoNI 694 CCTGTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1226 ATGCAT ATGCA^T HindIII 2071 AAGCTT A^AGCTT KpnI 2227 GGTACC GGTAC^C MflI 762 AGATCT [GA]^GATC[CT] MroI 116 TCCGGA T^CCGGA NcoI 1864 CCATGG C^CATGG NdeI 578 CATATG CA^TATG NsiI 1226 ATGCAT ATGCA^T NspV 2303 TTCGAA TT^CGAA PflMI 274 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2534 CACGTG CAC^GTG PmlI 2534 CACGTG CAC^GTG PstI 1205 CTGCAG CTGCA^G ScaI 1542 AGTACT AGT^ACT SexAI 749 ACCTGGT A^CC[AT]GGT SfuI 2303 TTCGAA TT^CGAA SmlI 2778 CTCAAG C^T[CT][GA]AG SspI 2788 AATATT AAT^ATT Van91I 274 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2898 TCTAGA T^CTAGA XcmI 1506 CCATGCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 762 AGATCT [GA]^GATC[CT] XmnI 2563 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 58 CGCG CG^CG 219 CGCG AlwI 2344 GATCC GGATC(4/5) 1210 GGATC AspHI 324 GTGCAC G[AT]GC[AT]^C 2463 GTGCTC AvaI 47 CTCGGG C^[CT]CG[GA]G 133 CCCGAG BalI 1389 TGGCCA TGG^CCA 2643 TGGCCA BciVI 2097 GGATAC GTATCC(6/5) 891 GTATCC BsaAI 1930 CACGTA [CT]AC^GT[GA] 2534 CACGTG BseRI 136 GAGGAG GAGGAG(10/8) 476 GAGGAG BsiHKAI 324 GTGCAC G[AT]GC[AT]^C 2463 GTGCTC BsmFI 155 GGGAC GGGAC(10/14) 2220 GGGAC BsoBI 47 CTCGGG C^[CT]CG[GA]G 133 CCCGAG BspHI 873 TCATGA T^CATGA 1701 TCATGA BspLU11I 1425 ACATGT A^CATGT 2648 ACATGT BstAPI 2257 GCATCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2841 GCAAAAGTTGC BstEII 745 GGTGACC G^GT[ACGT]ACC 1285 GGTGACC BstPI 745 GGTGACC G^GT[ACGT]ACC 1285 GGTGACC BstUI 58 CGCG CG^CG 219 CGCG BstXI 1275 CCAAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1858 CCAAGACCATGG EcoO65I 745 GGTGACC G^GT[ACGT]ACC 1285 GGTGACC HincII 1429 GTTGAC GT[CT]^[GA]AC 2945 GTCAAC HindII 1429 GTTGAC GT[CT]^[GA]AC 2945 GTCAAC MluNI 1389 TGGCCA TGG^CCA 2643 TGGCCA MscI 1389 TGGCCA TGG^CCA 2643 TGGCCA MvnI 58 CGCG CG^CG 219 CGCG PvuII 167 CAGCTG CAG^CTG 2621 CAGCTG RcaI 873 TCATGA T^CATGA 1701 TCATGA SfcI 678 CTACAG C^T[GA][CT]AG 1205 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AfaI AflIII AluI AvaII BanI BbvI BcnI BfaI BmyI BpmI BsaBI BsaJI BsgI BsiYI BslI BsmAI BsmI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoRV EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I