(Circular) (Six-base) MAPSORT of: pFN21AB8559.seq Check: 5554 from: 1 to: 7723 pFN21AB8559 7723 bp With 182 enzymes: SgfI * March 28, 2012 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2453 4058 2453 Size: 1605 6118 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5533 278 Size: 53 83 186 936 3997 2468 Fragments arranged by size: 3997 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5066 1937 Size: 3129 4594 Acc65I G'GTAC_C Cuts at: 4232 5044 4232 Size: 812 6911 AclI AA'CG_TT Cuts at: 1563 5850 6223 1563 Size: 4287 373 3063 Fragments arranged by size: 4287 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3102 3281 3708 4934 5786 7170 7438 1632 Size: 1470 179 427 1226 852 1384 268 1917 Fragments arranged by size: 1917 1470 1384 1226 852 427 268 179 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7526 AflIII A'CryG_T Cuts at: 1203 3430 4212 4540 4653 6622 7599 1203 Size: 2227 782 328 113 1969 977 1327 Fragments arranged by size: 2227 1969 1327 977 782 328 113 AgeI A'CCGG_T Cuts at: 6554 6554 Size: 7723 AhdI GACnn_n'nnGTC Cuts at: 6452 6452 Size: 7723 AleI CACnn'nnGTG Cuts at: 1166 4656 1166 Size: 3490 4233 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3828 1184 Size: 2644 5079 AlwNI CAG_nnn'CTG Cuts at: 5466 7038 5466 Size: 1572 6151 ApaLI G'TGCA_C Cuts at: 2329 5780 6936 2329 Size: 3451 1156 3116 Fragments arranged by size: 3451 3116 1156 ApoI r'AATT_y Cuts at: 1493 5032 5258 5410 7519 1493 Size: 3539 226 152 2109 1697 Fragments arranged by size: 3539 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6277 160 Size: 872 5245 1606 Fragments arranged by size: 5245 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 AvaI C'yCGr_G Cuts at: 1870 1943 2052 2138 5048 1870 Size: 73 109 86 2910 4545 Fragments arranged by size: 4545 2910 109 86 73 BamHI G'GATC_C Cuts at: 5053 5420 5053 Size: 367 7356 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 2465 2990 4070 4232 Size: 325 217 627 327 351 525 1080 162 Cuts at: 4232 5044 6499 618 Size: 812 1455 1842 Fragments arranged by size: 1842 1455 1080 812 627 525 351 327 325 217 162 BanII G_rGCy'C Cuts at: 1391 2138 5042 1391 Size: 747 2904 4072 Fragments arranged by size: 4072 2904 747 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3650 928 Size: 971 1751 5001 Fragments arranged by size: 5001 1751 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5914 5948 1903 Size: 34 3977 34 3678 Fragments arranged by size: 3977 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2907 4096 5615 6825 2907 Size: 1189 1519 1210 3805 Fragments arranged by size: 3805 1519 1210 1189 BclI T'GATC_A Cuts at: 1548 1548 Size: 7723 BfrBI ATG'CAT Cuts at: 3233 3233 Size: 7723 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2112 6334 136 Size: 107 122 71 631 605 440 4222 1525 Fragments arranged by size: 4222 1525 631 605 440 122 107 71 BglII A'GATC_T Cuts at: 2767 7718 2767 Size: 4951 2772 BlpI GC'TnA_GC Cuts at: 5140 5140 Size: 7723 Bme1580I G_kGCm'C Cuts at: 1790 2117 2333 4895 5784 6940 1790 Size: 327 216 2562 889 1156 2573 Fragments arranged by size: 2573 2562 1156 889 327 216 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7723 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3666 3825 5454 Size: 416 53 83 399 24 2249 159 1629 Cuts at: 5454 6412 442 Size: 958 1753 Fragments arranged by size: 2249 1753 1629 958 416 399 159 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7723 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4506 4506 Size: 7723 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2575 3662 3780 6383 1239 Size: 1336 1087 118 2603 2579 Fragments arranged by size: 2603 2579 1336 1087 118 Bpu10I CC'TnA_GC Cuts at: 2973 4301 2973 Size: 1328 6395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4768 5848 6713 7011 4768 Size: 1080 865 298 5480 Fragments arranged by size: 5480 1080 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4956 6386 882 Size: 617 3457 1430 2219 Fragments arranged by size: 3457 2219 1430 617 BsaAI yAC'GTr Cuts at: 493 1204 3937 4541 5514 493 Size: 711 2733 604 973 2702 Fragments arranged by size: 2733 2702 973 711 604 BsaBI GATnn'nnATC Cuts at: 2355 2874 4353 7717 2355 Size: 519 1479 3364 2361 Fragments arranged by size: 3364 2361 1479 519 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5530 5912 6547 275 Size: 53 83 186 936 3997 382 635 1451 Fragments arranged by size: 3997 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2121 6156 6554 6828 6975 7386 2121 Size: 4035 398 274 147 411 2458 Fragments arranged by size: 4035 2458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2174 2204 3753 3783 4140 Size: 30 165 30 771 30 1549 30 357 Cuts at: 4140 4170 1178 Size: 30 4731 Fragments arranged by size: 4731 1549 771 357 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 2496 1186 Size: 970 340 6413 Fragments arranged by size: 6413 970 340 BseYI C'CCAG_C Cuts at: 6926 7422 6926 Size: 496 7227 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2325 3000 3798 4086 4278 4718 1163 Size: 573 589 675 798 288 192 440 4168 Fragments arranged by size: 4168 798 675 589 573 440 288 192 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5173 5934 6962 1540 Size: 275 186 3172 761 1028 2301 Fragments arranged by size: 3172 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2333 4472 5784 5869 6940 2333 Size: 2139 1312 85 1071 3116 Fragments arranged by size: 3116 2139 1312 1071 85 BsmI GAATG_Cn' Cuts at: 2289 3580 4305 5234 5327 2289 Size: 1291 725 929 93 4685 Fragments arranged by size: 4685 1291 929 725 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2138 2333 4472 4895 5042 5784 Size: 399 327 21 195 2139 423 147 742 Cuts at: 5784 5869 6940 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 2139 1071 742 423 399 327 195 147 85 21 BspEI T'CCGG_A Cuts at: 2121 7386 2121 Size: 5265 2458 BspHI T'CATG_A Cuts at: 2878 3706 5612 7342 2878 Size: 828 1906 1730 3259 Fragments arranged by size: 3259 1906 1730 828 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2273 2453 4058 5078 844 Size: 1429 180 1605 1020 3489 Fragments arranged by size: 3489 1605 1429 1020 180 BsrBI CCG'CTC Cuts at: 1394 1823 5610 1394 Size: 429 3787 3507 Fragments arranged by size: 3787 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3235 3392 3843 6218 6392 65 Size: 1183 1987 157 451 2375 174 1396 Fragments arranged by size: 2375 1987 1396 1183 451 174 157 BsrFI r'CCGG_y Cuts at: 1700 1810 2231 6367 6554 1700 Size: 110 421 4136 187 2869 Fragments arranged by size: 4136 2869 421 187 110 BsrGI T'GTAC_A Cuts at: 96 2312 2785 3019 96 Size: 2216 473 234 4800 Fragments arranged by size: 4800 2216 473 234 BssHII G'CGCG_C Cuts at: 1925 2223 1925 Size: 298 7425 BssSI C'ACGA_G Cuts at: 5783 6795 5783 Size: 1012 6711 BstAPI GCAn_nnn'nTGC Cuts at: 794 4268 4852 794 Size: 3474 584 3665 Fragments arranged by size: 3665 3474 584 BstBI TT'CG_AA Cuts at: 4309 6527 4309 Size: 2218 5505 BstEII G'GTnAC_C Cuts at: 1184 2750 3290 1184 Size: 1566 540 5617 Fragments arranged by size: 5617 1566 540 BstXI CCAn_nnnn'nTGG Cuts at: 3287 3870 3287 Size: 583 7140 BstYI r'GATC_y Cuts at: 1972 2767 5053 5420 5805 5822 7274 7360 7372 Size: 795 2286 367 385 17 1452 86 12 Cuts at: 7372 7718 1972 Size: 346 1977 Fragments arranged by size: 2286 1977 1452 795 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3869 513 Size: 552 2804 4367 Fragments arranged by size: 4367 2804 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2354 3101 487 Size: 683 56 1128 747 5109 Fragments arranged by size: 5109 1128 747 683 56 BtsI GCAGTG_nn' Cuts at: 2501 2692 4706 5239 6033 6053 2501 Size: 191 2014 533 794 20 4171 Fragments arranged by size: 4171 2014 794 533 191 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7723 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7688 DraI TTT'AAA Cuts at: 3147 5027 3147 Size: 1880 5843 DraIII CAC_nnn'GTG Cuts at: 2337 2337 Size: 7723 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6730 809 Size: 507 5414 1802 Fragments arranged by size: 5414 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3112 3394 4648 Size: 54 1425 50 275 18 1282 282 1254 Cuts at: 4648 5170 6059 7688 8 Size: 522 889 1629 43 Fragments arranged by size: 1629 1425 1282 1254 889 522 282 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5170 1537 Size: 275 3358 4090 Fragments arranged by size: 4090 3358 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3708 4247 4449 4475 4717 5653 1353 Size: 2355 539 202 26 242 936 3423 Fragments arranged by size: 3423 2355 936 539 242 202 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6310 6684 6830 387 Size: 1064 138 4721 374 146 1280 Fragments arranged by size: 4721 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2575 3102 3281 3662 3708 3780 4934 Size: 393 943 527 179 381 46 72 1154 Cuts at: 4934 5786 6383 7170 7438 1239 Size: 852 597 787 268 1524 Fragments arranged by size: 1524 1154 943 852 787 597 527 393 381 268 179 72 46 EcoNI CCTnn'n_nnAGG Cuts at: 2703 2703 Size: 7723 EcoRI G'AATT_C Cuts at: 5032 5410 5032 Size: 378 7345 EcoRV GAT'ATC Cuts at: 3736 3985 4780 3736 Size: 249 795 6679 Fragments arranged by size: 6679 795 249 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3107 3371 3647 4746 3107 Size: 264 276 1099 6084 Fragments arranged by size: 6084 1099 276 264 FspI TGC'GCA Cuts at: 6229 6229 Size: 7723 HaeII r_GCGC'y Cuts at: 1678 2052 3537 3592 6870 1678 Size: 374 1485 55 3278 2531 Fragments arranged by size: 3278 2531 1485 374 55 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 2908 2940 3166 Size: 32 135 32 1052 32 252 32 226 Cuts at: 3166 3198 4766 4798 4963 4995 6364 6396 6438 Size: 32 1568 32 165 32 1369 32 42 Cuts at: 6438 6470 1373 Size: 32 2626 Fragments arranged by size: 2626 1568 1369 1052 252 226 165 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3436 4952 5067 5313 669 Size: 988 281 1498 1516 115 246 3079 Fragments arranged by size: 3079 1516 1498 988 281 246 115 HindIII A'AGCT_T Cuts at: 748 4076 748 Size: 3328 4395 HpaI GTT'AAC Cuts at: 5313 5313 Size: 7723 KpnI G_GTAC'C Cuts at: 4236 5048 4236 Size: 812 6911 MfeI C'AATT_G Cuts at: 5322 5322 Size: 7723 MluI A'CGCG_T Cuts at: 7599 7599 Size: 7723 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3119 6837 7021 1305 Size: 1814 3718 184 2007 Fragments arranged by size: 3718 2007 1814 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3396 4650 10 Size: 54 1425 343 1564 1254 3083 Fragments arranged by size: 3083 1564 1425 1254 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2805 3404 4656 5681 6040 6199 518 Size: 648 1639 599 1252 1025 359 159 2042 Fragments arranged by size: 2042 1639 1252 1025 648 599 359 159 MspA1I CmG'CkG Cuts at: 1606 2174 2187 4628 5139 5816 6964 7209 1606 Size: 568 13 2441 511 677 1148 245 2120 Fragments arranged by size: 2441 2120 1148 677 568 511 245 13 NcoI C'CATG_G Cuts at: 513 1065 3869 513 Size: 552 2804 4367 Fragments arranged by size: 4367 2804 552 NdeI CA'TA_TG Cuts at: 387 2584 387 Size: 2197 5526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7723 NotI GC'GGCC_GC Cuts at: 5170 5170 Size: 7723 NsiI A_TGCA'T Cuts at: 3235 3235 Size: 7723 NspI r_CATG'y Cuts at: 1132 1229 2641 3434 4657 5081 6626 1132 Size: 97 1412 793 1223 424 1545 2229 Fragments arranged by size: 2229 1545 1412 1223 793 424 97 PasI CC'CwG_GG Cuts at: 1348 4237 1348 Size: 2889 4834 PciI A'CATG_T Cuts at: 3430 4653 6622 3430 Size: 1223 1969 4531 Fragments arranged by size: 4531 1969 1223 PflMI CCAn_nnn'nTGG Cuts at: 1108 2285 1108 Size: 1177 6546 PfoI T'CCnGG_A Cuts at: 2015 2214 2547 2658 3678 4580 2015 Size: 199 333 111 1020 902 5158 Fragments arranged by size: 5158 1020 902 333 199 111 PmeI GTTT'AAAC Cuts at: 5027 5027 Size: 7723 PmlI CAC'GTG Cuts at: 4541 4541 Size: 7723 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5792 5824 7305 7337 5792 Size: 32 1481 32 6178 Fragments arranged by size: 6178 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7723 PsiI TTA'TAA Cuts at: 5293 5293 Size: 7723 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3942 3974 3942 Size: 32 7691 PstI C_TGCA'G Cuts at: 830 3214 5075 830 Size: 2384 1861 3478 Fragments arranged by size: 3478 2384 1861 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7723 PvuII CAG'CTG Cuts at: 2174 4628 2174 Size: 2454 5269 SalI G'TCGA_C Cuts at: 1936 5065 1936 Size: 3129 4594 SbfI CC_TGCA'GG Cuts at: 5075 5075 Size: 7723 ScaI AGT'ACT Cuts at: 3549 5971 7683 3549 Size: 2422 1712 3589 Fragments arranged by size: 3589 2422 1712 SexAI A'CCwGG_T Cuts at: 2754 2754 Size: 7723 SfcI C'TryA_G Cuts at: 826 1045 2683 3210 5071 6206 6887 7078 826 Size: 219 1638 527 1861 1135 681 191 1471 Fragments arranged by size: 1861 1638 1471 1135 681 527 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7723 SmaI CCC'GGG Cuts at: 1872 5050 1872 Size: 3178 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4783 5827 6728 6990 820 Size: 197 926 2840 1044 901 262 1553 Fragments arranged by size: 2840 1553 1044 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5514 493 Size: 5021 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7723 SphI G_CATG'C Cuts at: 1132 5081 1132 Size: 3949 3774 SspI AAT'ATT Cuts at: 5 52 4795 5647 5 Size: 47 4743 852 2081 Fragments arranged by size: 4743 2081 852 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7723 StyI C'CwwG_G Cuts at: 513 1065 2667 3198 3869 5162 513 Size: 552 1602 531 671 1293 3074 Fragments arranged by size: 3074 1602 1293 671 552 531 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2999 5750 5767 5920 6105 6550 325 Size: 1255 1419 2751 17 153 185 445 1498 Fragments arranged by size: 2751 1498 1419 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2312 2785 2890 Size: 274 80 33 51 1443 335 473 105 Cuts at: 2890 3019 3118 3547 5969 7681 96 Size: 129 99 429 2422 1712 138 Fragments arranged by size: 2422 1712 1443 473 429 335 274 138 129 105 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2063 2095 2480 2512 2719 2751 7683 7715 2063 Size: 32 385 32 207 32 4932 32 2071 Fragments arranged by size: 4932 2071 385 207 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7723 XbaI T'CTAG_A Cuts at: 4903 5059 4903 Size: 156 7567 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3518 3518 Size: 7723 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7723 XmaI C'CCGG_G Cuts at: 1870 5048 1870 Size: 3178 4545 XmnI GAAnn'nnTTC Cuts at: 4572 5852 4572 Size: 1280 6443 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5531 276 Size: 53 83 186 936 3997 2468 Fragments arranged by size: 3997 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI