(Linear) (Six-base) MAPSORT of: KIBB8559.seq Check: 3143 from: 1 to: 3018 KIBB8559.orf With 182 enzymes: SgfI * March 22, 2012 15:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 448 2053 3018 Size: 448 1605 965 Fragments arranged by size: 1605 965 448 Acc65I G'GTAC_C Cuts at: 0 2227 3018 Size: 2227 791 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1097 1276 1703 2929 3018 Size: 1097 179 427 1226 89 Fragments arranged by size: 1226 1097 427 179 89 AflIII A'CryG_T Cuts at: 0 1425 2207 2535 2648 3018 Size: 1425 782 328 113 370 Fragments arranged by size: 1425 782 370 328 113 AleI CACnn'nnGTG Cuts at: 0 2651 3018 Size: 2651 367 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1823 3018 Size: 1823 1195 ApaLI G'TGCA_C Cuts at: 0 324 3018 Size: 324 2694 AvaI C'yCGr_G Cuts at: 0 47 133 3018 Size: 47 86 2885 Fragments arranged by size: 2885 86 47 BanI G'GyrC_C Cuts at: 0 109 460 985 2065 2227 3018 Size: 109 351 525 1080 162 791 Fragments arranged by size: 1080 791 525 351 162 109 BanII G_rGCy'C Cuts at: 0 133 3018 Size: 133 2885 BbsI GAAGACnn'nnnn_ Cuts at: 0 1645 3018 Size: 1645 1373 BciVI GTATCCnnnnn_n' Cuts at: 0 902 2091 3018 Size: 902 1189 927 Fragments arranged by size: 1189 927 902 BfrBI ATG'CAT Cuts at: 0 1228 3018 Size: 1228 1790 BglI GCCn_nnn'nGGC Cuts at: 0 107 3018 Size: 107 2911 BglII A'GATC_T Cuts at: 0 762 3018 Size: 762 2256 Bme1580I G_kGCm'C Cuts at: 0 112 328 2890 3018 Size: 112 216 2562 128 Fragments arranged by size: 2562 216 128 112 BmrI ACTGGGnnnn_n' Cuts at: 0 1661 1820 3018 Size: 1661 159 1198 Fragments arranged by size: 1661 1198 159 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2501 3018 Size: 2501 517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 570 1657 1775 3018 Size: 570 1087 118 1243 Fragments arranged by size: 1243 1087 570 118 Bpu10I CC'TnA_GC Cuts at: 0 968 2296 3018 Size: 968 1328 722 Fragments arranged by size: 1328 968 722 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2763 3018 Size: 2763 255 BsaI GGTCTCn'nnnn_ Cuts at: 0 2951 3018 Size: 2951 67 BsaAI yAC'GTr Cuts at: 0 1932 2536 3018 Size: 1932 604 482 Fragments arranged by size: 1932 604 482 BsaBI GATnn'nnATC Cuts at: 0 350 869 2348 3018 Size: 350 519 1479 670 Fragments arranged by size: 1479 670 519 350 BsaWI w'CCGG_w Cuts at: 0 116 3018 Size: 116 2902 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 169 199 1748 1778 2135 2165 3018 Size: 169 30 1549 30 357 30 853 Fragments arranged by size: 1549 853 357 169 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 151 491 3018 Size: 151 340 2527 Fragments arranged by size: 2527 340 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 320 995 1793 2081 2273 2713 3018 Size: 320 675 798 288 192 440 305 Fragments arranged by size: 798 675 440 320 305 288 192 BsiHKAI G_wGCw'C Cuts at: 0 328 2467 3018 Size: 328 2139 551 Fragments arranged by size: 2139 551 328 BsmI GAATG_Cn' Cuts at: 0 284 1575 2300 3018 Size: 284 1291 725 718 Fragments arranged by size: 1291 725 718 284 Bsp1286I G_dGCh'C Cuts at: 0 112 133 328 2467 2890 3018 Size: 112 21 195 2139 423 128 Fragments arranged by size: 2139 423 195 128 112 21 BspEI T'CCGG_A Cuts at: 0 116 3018 Size: 116 2902 BspHI T'CATG_A Cuts at: 0 873 1701 3018 Size: 873 828 1317 Fragments arranged by size: 1317 873 828 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 268 448 2053 3018 Size: 268 180 1605 965 Fragments arranged by size: 1605 965 268 180 BsrDI GCAATG_nn' Cuts at: 0 1230 1387 1838 3018 Size: 1230 157 451 1180 Fragments arranged by size: 1230 1180 451 157 BsrFI r'CCGG_y Cuts at: 0 226 3018 Size: 226 2792 BsrGI T'GTAC_A Cuts at: 0 307 780 1014 3018 Size: 307 473 234 2004 Fragments arranged by size: 2004 473 307 234 BssHII G'CGCG_C Cuts at: 0 218 3018 Size: 218 2800 BstAPI GCAn_nnn'nTGC Cuts at: 0 2263 2847 3018 Size: 2263 584 171 Fragments arranged by size: 2263 584 171 BstBI TT'CG_AA Cuts at: 0 2304 3018 Size: 2304 714 BstEII G'GTnAC_C Cuts at: 0 745 1285 3018 Size: 745 540 1733 Fragments arranged by size: 1733 745 540 BstXI CCAn_nnnn'nTGG Cuts at: 0 1282 1865 3018 Size: 1282 583 1153 Fragments arranged by size: 1282 1153 583 BstYI r'GATC_y Cuts at: 0 762 3018 Size: 762 2256 BtgI C'CryG_G Cuts at: 0 1864 3018 Size: 1864 1154 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 349 1096 3018 Size: 349 747 1922 Fragments arranged by size: 1922 747 349 BtsI GCAGTG_nn' Cuts at: 0 496 687 2701 3018 Size: 496 191 2014 317 Fragments arranged by size: 2014 496 317 191 DraI TTT'AAA Cuts at: 0 1142 3018 Size: 1142 1876 DraIII CAC_nnn'GTG Cuts at: 0 332 3018 Size: 332 2686 EaeI y'GGCC_r Cuts at: 0 1107 1389 2643 3018 Size: 1107 282 1254 375 Fragments arranged by size: 1254 1107 375 282 EarI CTCTTCn'nnn_ Cuts at: 0 1703 2242 2444 2470 2712 3018 Size: 1703 539 202 26 242 306 Fragments arranged by size: 1703 539 306 242 202 26 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 570 1097 1276 1657 1703 1775 2929 3018 Size: 570 527 179 381 46 72 1154 89 Fragments arranged by size: 1154 570 527 381 179 89 72 46 EcoNI CCTnn'n_nnAGG Cuts at: 0 698 3018 Size: 698 2320 EcoRV GAT'ATC Cuts at: 0 1731 1980 2775 3018 Size: 1731 249 795 243 Fragments arranged by size: 1731 795 249 243 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1102 1366 1642 2741 3018 Size: 1102 264 276 1099 277 Fragments arranged by size: 1102 1099 277 276 264 HaeII r_GCGC'y Cuts at: 0 47 1532 1587 3018 Size: 47 1485 55 1431 Fragments arranged by size: 1485 1431 55 47 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 619 651 903 935 1161 1193 2761 2793 Size: 619 32 252 32 226 32 1568 32 Cuts at: 2793 2958 2990 3018 Size: 165 32 28 Fragments arranged by size: 1568 619 252 226 165 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 0 1431 2947 3018 Size: 1431 1516 71 Fragments arranged by size: 1516 1431 71 HindIII A'AGCT_T Cuts at: 0 2071 3018 Size: 2071 947 KpnI G_GTAC'C Cuts at: 0 2231 3018 Size: 2231 787 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1114 3018 Size: 1114 1904 MscI TGG'CCA Cuts at: 0 1391 2645 3018 Size: 1391 1254 373 Fragments arranged by size: 1391 1254 373 MslI CAynn'nnrTG Cuts at: 0 800 1399 2651 3018 Size: 800 599 1252 367 Fragments arranged by size: 1252 800 599 367 MspA1I CmG'CkG Cuts at: 0 169 182 2623 3018 Size: 169 13 2441 395 Fragments arranged by size: 2441 395 169 13 NcoI C'CATG_G Cuts at: 0 1864 3018 Size: 1864 1154 NdeI CA'TA_TG Cuts at: 0 579 3018 Size: 579 2439 NsiI A_TGCA'T Cuts at: 0 1230 3018 Size: 1230 1788 NspI r_CATG'y Cuts at: 0 636 1429 2652 3018 Size: 636 793 1223 366 Fragments arranged by size: 1223 793 636 366 PasI CC'CwG_GG Cuts at: 0 2232 3018 Size: 2232 786 PciI A'CATG_T Cuts at: 0 1425 2648 3018 Size: 1425 1223 370 Fragments arranged by size: 1425 1223 370 PflMI CCAn_nnn'nTGG Cuts at: 0 280 3018 Size: 280 2738 PfoI T'CCnGG_A Cuts at: 0 10 209 542 653 1673 2575 3018 Size: 10 199 333 111 1020 902 443 Fragments arranged by size: 1020 902 443 333 199 111 10 PmlI CAC'GTG Cuts at: 0 2536 3018 Size: 2536 482 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1937 1969 3018 Size: 1937 32 1049 Fragments arranged by size: 1937 1049 32 PstI C_TGCA'G Cuts at: 0 1209 3018 Size: 1209 1809 PvuII CAG'CTG Cuts at: 0 169 2623 3018 Size: 169 2454 395 Fragments arranged by size: 2454 395 169 ScaI AGT'ACT Cuts at: 0 1544 3018 Size: 1544 1474 SexAI A'CCwGG_T Cuts at: 0 749 3018 Size: 749 2269 SfcI C'TryA_G Cuts at: 0 678 1205 3018 Size: 678 527 1813 Fragments arranged by size: 1813 678 527 SmlI C'TyrA_G Cuts at: 0 2778 3018 Size: 2778 240 SspI AAT'ATT Cuts at: 0 2790 3018 Size: 2790 228 StyI C'CwwG_G Cuts at: 0 662 1193 1864 3018 Size: 662 531 671 1154 Fragments arranged by size: 1154 671 662 531 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 994 3018 Size: 994 2024 TatI w'GTAC_w Cuts at: 0 307 780 885 1014 1113 1542 3018 Size: 307 473 105 129 99 429 1476 Fragments arranged by size: 1476 473 429 307 129 105 99 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 58 90 475 507 714 746 3018 Size: 58 32 385 32 207 32 2272 Fragments arranged by size: 2272 385 207 58 32 32 32 XbaI T'CTAG_A Cuts at: 0 2898 3018 Size: 2898 120 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1513 3018 Size: 1513 1505 XmnI GAAnn'nnTTC Cuts at: 0 2567 3018 Size: 2567 451 Enzymes that do cut: AarI Acc65I AcuI AflIII AleI AlfI ApaLI AvaI BanI BanII BbsI BciVI BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII EaeI EarI Eco57MI EcoNI EcoRV FalI HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI PfoI PmlI PsrI PstI PvuII ScaI SexAI SfcI SmlI SspI StyI TaqII TatI TstI XbaI XcmI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI BlpI BmgBI BmtI BsaHI BseYI BsiEI BsiWI BsmBI BsrBI BssSI BstZ17I Bsu36I ClaI CspCI DrdI EagI EciI EcoICRI EcoO109I EcoRI FseI FspI FspAI HpaI KasI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI ZraI