################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp718I AviII AvrII BamHI BanII BbeI BbrPI BbvCI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 12 GTCTAC GT^[AC][GT]AC AccII 472 CGCG CG^CG AcsI 190 AAATTC [GA]^AATT[CT] AhdI 841 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 23 GGATC GGATC(4/5) Aor51HI 497 AGCGCT AGC^GCT ApoI 190 AAATTC [GA]^AATT[CT] AspEI 841 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 854 GTGCTC G[AT]GC[AT]^C AspI 862 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 346 CTCGGG C^[CT]CG[GA]G BanI 724 GGCACC G^G[CT][GA]CC BcgI 437 GCACCTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 543 CCGGG CC^[GC]GG BglI 476 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 210 AGATCT A^GATCT BmyI 854 GTGCTC G[AGT]GC[ACT]^C BpmI 430 CTGGAG CTGGAG(16/14) BsgI 466 GTGCAG GTGCAG(16/14) BsiHKAI 854 GTGCTC G[AT]GC[AT]^C BsmI 746 GCATTC GAATGC(1/-1) BsoBI 346 CTCGGG C^[CT]CG[GA]G Bsp1286I 854 GTGCTC G[AGT]GC[ACT]^C BspHI 858 TCATGA T^CATGA BsrDI 99 CATTGC GCAATG(2/0) BssSI 523 CTCGTG CACGAG(-5/-1) BstF5I 384 CATCC GGATG(2/0) BstUI 472 CGCG CG^CG BstYI 210 AGATCT [GA]^GATC[CT] DraII 42 GGGGCCT [GA]G^G[ACGT]CC[CT] Eam1105I 841 GACTACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 497 AGCGCT AGC^GCT EcoO109I 42 GGGGCCT [GA]G^G[ACGT]CC[CT] FauI 126 GCGGG CCCGC(4/6) FokI 384 CATCC GGATG(9/13) HaeII 497 AGCGCT [GA]GCGC^[CT] HindIII 200 AAGCTT A^AGCTT MflI 210 AGATCT [GA]^GATC[CT] MseI 821 TTAA T^TAA MslI 772 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 124 CAGCGG C[AC]G^C[GT]G MvnI 472 CGCG CG^CG NaeI 259 GCCGGC GCC^GGC NciI 543 CCGGG CC^[GC]GG NgoMIV 259 GCCGGC G^CCGGC NspI 275 ACATGC [GA]CATG^[CT] PflFI 862 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 362 CTGCAG CTGCA^G RcaI 858 TCATGA T^CATGA SfaNI 687 GATGC GCATC(5/9) SfcI 362 CTGCAG C^T[GA][CT]AG TaqI 503 TCGA T^CGA Tru9I 821 TTAA T^TAA Tsp509I 191 AATT ^AATT TspRI 777 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 862 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 503 TCGA T^CGA XcmI 419 CCATGGCCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 210 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 92 GTAC GT^AC 618 GTAC AluI 58 AGCT AG^CT 201 AGCT BbsI 327 GAAGAC GAAGAC(2/6) 672 GAAGAC BbvI 468 GCAGC GCAGC(8/12) 567 GCAGC BpuAI 327 GAAGAC GAAGAC(2/6) 672 GAAGAC BsaI 352 GAGACC GGTCTC(1/5) 697 GAGACC BsmFI 379 GGGAC GGGAC(10/14) 737 GGGAC BsrI 834 ACTGG ACTGG(1/-1) 863 ACTGG DdeI 282 CTCAG C^T[ACGT]AG 627 CTCAG HincII 232 GTTGAC GT[CT]^[GA]AC 838 GTTGAC HindII 232 GTTGAC GT[CT]^[GA]AC 838 GTTGAC MaeII 230 ACGT A^CGT 665 ACGT PflMI 299 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 644 CCATGGTCTGG PleI 367 GACTC GAGTC(4/5) 338 GAGTC RsaI 92 GTAC GT^AC 618 GTAC SmlI 54 CTCAAG C^T[CT][GA]AG 217 CTCAAG TseI 468 GCAGC G^C[AT]GC 567 GCAGC Van91I 299 CCATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 644 CCATGGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI Asp700I AvaII BalI BsaJI BsiYI BslI BsmAI BsrFI BssKI BstNI Cac8I CfoI Cfr10I Cfr13I DpnII DsaI EaeI EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MluNI MnlI MscI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TfiI XmnI