Restriction map for
pFN21AB7963
(Circular) (Six-base) MAPSORT of: pFN21AB7963.seq Check: 6218 from: 1 to: 5581
pFN21AB7963 5581 bp
With 182 enzymes: SgfI *
March 28, 2012 12:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3391 278
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2018 2924 1937
Size: 81 906 4594
Fragments arranged by size:
4594 906 81
Acc65I G'GTAC_C
Cuts at: 2902 2902
Size: 5581
AclI AA'CG_TT
Cuts at: 1563 3708 4081 1563
Size: 2145 373 3063
Fragments arranged by size:
3063 2145 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2264 2577 2588 2853 3644 5028 5296 1632
Size: 632 313 11 265 791 1384 268 1917
Fragments arranged by size:
1917 1384 791 632 313 268 265 11
AfeI AGC'GCT
Cuts at: 2504 2504
Size: 5581
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5384
AflIII A'CryG_T
Cuts at: 1203 4480 5457 1203
Size: 3277 977 1327
Fragments arranged by size:
3277 1327 977
AgeI A'CCGG_T
Cuts at: 4412 4412
Size: 5581
AhdI GACnn_n'nnGTC
Cuts at: 2851 4310 2851
Size: 1459 4122
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5581
AlwNI CAG_nnn'CTG
Cuts at: 3324 4896 3324
Size: 1572 4009
ApaLI G'TGCA_C
Cuts at: 3638 4794 3638
Size: 1156 4425
ApoI r'AATT_y
Cuts at: 1493 2195 2890 3116 3268 5377 1493
Size: 702 695 226 152 2109 1697
Fragments arranged by size:
2109 1697 702 695 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4135 160
Size: 872 3103 1606
Fragments arranged by size:
3103 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
AvaI C'yCGr_G
Cuts at: 1870 1943 2351 2906 1870
Size: 73 408 555 4545
Fragments arranged by size:
4545 555 408 73
BamHI G'GATC_C
Cuts at: 2911 3278 2911
Size: 367 5214
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2729 2902 4357 618
Size: 325 217 627 942 173 1455 1842
Fragments arranged by size:
1842 1455 942 627 325 217 173
BanII G_rGCy'C
Cuts at: 1391 2900 1391
Size: 1509 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2339 2684 928
Size: 971 440 345 3825
Fragments arranged by size:
3825 971 440 345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2431 2465 3772 3806 1903
Size: 34 494 34 1307 34 3678
Fragments arranged by size:
3678 1307 494 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3473 4683 3473
Size: 1210 4371
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2487 4192 136
Size: 107 122 71 631 605 815 1705 1525
Fragments arranged by size:
1705 1525 815 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2215 5576 2215
Size: 3361 2220
BlpI GC'TnA_GC
Cuts at: 2998 2998
Size: 5581
Bme1580I G_kGCm'C
Cuts at: 1790 3642 4798 1790
Size: 1852 1156 2573
Fragments arranged by size:
2573 1852 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2878 3312 4270
Size: 416 53 83 399 24 1461 434 958
Cuts at: 4270 442
Size: 1753
Fragments arranged by size:
1753 1461 958 434 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5581
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2366 2366
Size: 5581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2456 4241 1239
Size: 1217 1785 2579
Fragments arranged by size:
2579 1785 1217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2044 2207 3706 4571 4869 2044
Size: 163 1499 865 298 2756
Fragments arranged by size:
2756 1499 865 298 163
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2351 2696 4244 882
Size: 617 852 345 1548 2219
Fragments arranged by size:
2219 1548 852 617 345
BsaAI yAC'GTr
Cuts at: 493 1204 3372 493
Size: 711 2168 2702
Fragments arranged by size:
2702 2168 711
BsaBI GATnn'nnATC
Cuts at: 5575 5575
Size: 5581
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3388 3770 4405 275
Size: 53 83 186 936 1855 382 635 1451
Fragments arranged by size:
1855 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4014 4412 4686 4833 5244 4014
Size: 398 274 147 411 4351
Fragments arranged by size:
4351 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5356
Fragments arranged by size:
5356 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5581
BseYI C'CCAG_C
Cuts at: 4784 5280 4784
Size: 496 5085
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2492 1163
Size: 573 756 4252
Fragments arranged by size:
4252 756 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3031 3792 4820 1540
Size: 275 186 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2863 3642 3727 4798 2863
Size: 779 85 1071 3646
Fragments arranged by size:
3646 1071 779 85
BsmI GAATG_Cn'
Cuts at: 2751 3092 3185 2751
Size: 341 93 5147
Fragments arranged by size:
5147 341 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2863 2900 3642 3727 4798 1391
Size: 399 1073 37 742 85 1071 2174
Fragments arranged by size:
2174 1073 1071 742 399 85 37
BspEI T'CCGG_A
Cuts at: 5244 5244
Size: 5581
BspHI T'CATG_A
Cuts at: 2863 3470 5200 2863
Size: 607 1730 3244
Fragments arranged by size:
3244 1730 607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2936 844
Size: 2092 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3468 1394
Size: 429 1645 3507
Fragments arranged by size:
3507 1645 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2103 4076 4250 65
Size: 1183 855 1973 174 1396
Fragments arranged by size:
1973 1396 1183 855 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2264 2293 2638 4225 4412 1700
Size: 110 454 29 345 1587 187 2869
Fragments arranged by size:
2869 1587 454 345 187 110 29
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5581
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5581
BssSI C'ACGA_G
Cuts at: 2528 3641 4653 2528
Size: 1113 1012 3456
Fragments arranged by size:
3456 1113 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5581
BstBI TT'CG_AA
Cuts at: 4385 4385
Size: 5581
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5581
BstYI r'GATC_y
Cuts at: 1972 2215 2911 3278 3663 3680 5132 5218 5230
Size: 243 696 367 385 17 1452 86 12
Cuts at: 5230 5576 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 696 385 367 346 243 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2079 2304 2392 2424 2649 513
Size: 552 939 75 225 88 32 225 3445
Fragments arranged by size:
3445 939 552 225 225 88 75 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2434 487
Size: 683 56 1208 3634
Fragments arranged by size:
3634 1208 683 56
BtsI GCAGTG_nn'
Cuts at: 2788 3097 3891 3911 2788
Size: 309 794 20 4458
Fragments arranged by size:
4458 794 309 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5581
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5546
DraI TTT'AAA
Cuts at: 2885 2885
Size: 5581
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4588 809
Size: 507 3272 1802
Fragments arranged by size:
3272 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2427 2532
Size: 54 1425 50 275 18 252 345 105
Cuts at: 2532 2646 3028 3917 5546 8
Size: 114 382 889 1629 43
Fragments arranged by size:
1629 1425 889 382 345 275 252 114
105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3028 1537
Size: 275 1216 4090
Fragments arranged by size:
4090 1216 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3511 1353
Size: 2158 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2168 2513 4168 4542 4688 387
Size: 1064 138 579 345 1655 374 146 1280
Fragments arranged by size:
1655 1280 1064 579 374 345 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2456 2577 2588 2853 3644 4241
Size: 393 632 192 121 11 265 791 597
Cuts at: 4241 5028 5296 1239
Size: 787 268 1524
Fragments arranged by size:
1524 791 787 632 597 393 268 265
192 121 11
EcoO109I rG'GnC_Cy
Cuts at: 2048 2048
Size: 5581
EcoRI G'AATT_C
Cuts at: 2890 3268 2890
Size: 378 5203
FspI TGC'GCA
Cuts at: 4087 4087
Size: 5581
HaeII r_GCGC'y
Cuts at: 1678 2506 4728 1678
Size: 828 2222 2531
Fragments arranged by size:
2531 2222 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2136 2168 2511 2543 2837
Size: 32 135 32 564 32 343 32 294
Cuts at: 2837 2869 4222 4254 4296 4328 1373
Size: 32 1353 32 42 32 2626
Fragments arranged by size:
2626 1353 564 343 294 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2239 2845 2925 3171 669
Size: 988 281 301 606 80 246 3079
Fragments arranged by size:
3079 988 606 301 281 246 80
HindIII A'AGCT_T
Cuts at: 748 2205 748
Size: 1457 4124
HpaI GTT'AAC
Cuts at: 3171 3171
Size: 5581
KpnI G_GTAC'C
Cuts at: 2906 2906
Size: 5581
MfeI C'AATT_G
Cuts at: 3180 3180
Size: 5581
MluI A'CGCG_T
Cuts at: 5457 5457
Size: 5581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4695 4879 1305
Size: 3390 184 2007
Fragments arranged by size:
3390 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2084 2429 2534 2648 10
Size: 54 1425 343 252 345 105 114 2943
Fragments arranged by size:
2943 1425 345 343 252 114 105 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2781 3539 3898 4057 518
Size: 648 1615 758 359 159 2042
Fragments arranged by size:
2042 1615 758 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2131 2997 3674 4822 5067 1606
Size: 525 866 677 1148 245 2120
Fragments arranged by size:
2120 1148 866 677 525 245
NaeI GCC'GGC
Cuts at: 2266 2266
Size: 5581
NcoI C'CATG_G
Cuts at: 513 1065 2004 2079 2304 2424 2649 513
Size: 552 939 75 225 120 225 3445
Fragments arranged by size:
3445 939 552 225 225 120 75
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5581
NgoMIV G'CCGG_C
Cuts at: 2264 2264
Size: 5581
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5581
NotI GC'GGCC_GC
Cuts at: 3028 3028
Size: 5581
NspI r_CATG'y
Cuts at: 1132 1229 2284 2939 4484 1132
Size: 97 1055 655 1545 2229
Fragments arranged by size:
2229 1545 1055 655 97
PasI CC'CwG_GG
Cuts at: 1348 2609 1348
Size: 1261 4320
PciI A'CATG_T
Cuts at: 4480 4480
Size: 5581
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2310 2655 1108
Size: 1202 345 4034
Fragments arranged by size:
4034 1202 345
PmeI GTTT'AAAC
Cuts at: 2885 2885
Size: 5581
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 5163 5195 3650
Size: 32 1481 32 4036
Fragments arranged by size:
4036 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5581
PsiI TTA'TAA
Cuts at: 3151 3151
Size: 5581
PstI C_TGCA'G
Cuts at: 830 2371 2933 830
Size: 1541 562 3478
Fragments arranged by size:
3478 1541 562
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5581
SalI G'TCGA_C
Cuts at: 1936 2923 1936
Size: 987 4594
SbfI CC_TGCA'GG
Cuts at: 2933 2933
Size: 5581
ScaI AGT'ACT
Cuts at: 3829 5541 3829
Size: 1712 3869
SfcI C'TryA_G
Cuts at: 826 1045 2367 2929 4064 4745 4936 826
Size: 219 1322 562 1135 681 191 1471
Fragments arranged by size:
1471 1322 1135 681 562 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5581
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5581
SmaI CCC'GGG
Cuts at: 1872 2908 1872
Size: 1036 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2059 2222 3685 4586 4848 820
Size: 197 926 116 163 1463 901 262 1553
Fragments arranged by size:
1553 1463 926 901 262 197 163 116
SnaBI TAC'GTA
Cuts at: 493 3372 493
Size: 2879 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5581
SphI G_CATG'C
Cuts at: 1132 2939 1132
Size: 1807 3774
SspI AAT'ATT
Cuts at: 5 52 3505 5
Size: 47 3453 2081
Fragments arranged by size:
3453 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5581
StyI C'CwwG_G
Cuts at: 513 1065 2004 2079 2304 2424 2649 3020 513
Size: 552 939 75 225 120 225 371 3074
Fragments arranged by size:
3074 939 552 371 225 225 120 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2610 3608 3625 3778 3963 4408 325
Size: 1255 1030 998 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1030 998 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2622 3827 5539
Size: 274 80 33 51 1443 645 1205 1712
Cuts at: 5539 96
Size: 138
Fragments arranged by size:
1712 1443 1205 645 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2377 2409 5541 5573 2377
Size: 32 3132 32 2385
Fragments arranged by size:
3132 2385 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2870 1316
Size: 1554 4027
XbaI T'CTAG_A
Cuts at: 2917 2917
Size: 5581
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2431 2431
Size: 5581
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5581
XmaI C'CCGG_G
Cuts at: 1870 2906 1870
Size: 1036 4545
XmnI GAAnn'nnTTC
Cuts at: 2174 2217 2519 3710 2174
Size: 43 302 1191 4045
Fragments arranged by size:
4045 1191 302 43
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3389 276
Size: 53 83 186 936 1855 2468
Fragments arranged by size:
2468 1855 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI