(Linear) (Six-base) MAPSORT of: KIBB7963.seq Check: 1114 from: 1 to: 876 KIBB7963.orf With 182 enzymes: SgfI * March 22, 2012 15:43 .. AccI GT'mk_AC Cuts at: 0 13 876 Size: 13 863 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 572 583 848 876 Size: 259 313 11 265 28 Fragments arranged by size: 313 265 259 28 11 AfeI AGC'GCT Cuts at: 0 499 876 Size: 499 377 AhdI GACnn_n'nnGTC Cuts at: 0 846 876 Size: 846 30 ApoI r'AATT_y Cuts at: 0 190 876 Size: 190 686 AvaI C'yCGr_G Cuts at: 0 346 876 Size: 346 530 BanI G'GyrC_C Cuts at: 0 724 876 Size: 724 152 BbsI GAAGACnn'nnnn_ Cuts at: 0 334 679 876 Size: 334 345 197 Fragments arranged by size: 345 334 197 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 426 460 876 Size: 426 34 416 Fragments arranged by size: 426 416 34 BglI GCCn_nnn'nGGC Cuts at: 0 482 876 Size: 482 394 BglII A'GATC_T Cuts at: 0 210 876 Size: 210 666 BmrI ACTGGGnnnn_n' Cuts at: 0 873 876 Size: 873 3 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 361 876 Size: 361 515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 451 876 Size: 451 425 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 39 202 876 Size: 39 163 674 Fragments arranged by size: 674 163 39 BsaI GGTCTCn'nnnn_ Cuts at: 0 346 691 876 Size: 346 345 185 Fragments arranged by size: 346 345 185 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 487 876 Size: 487 389 BsiHKAI G_wGCw'C Cuts at: 0 858 876 Size: 858 18 BsmI GAATG_Cn' Cuts at: 0 746 876 Size: 746 130 Bsp1286I G_dGCh'C Cuts at: 0 858 876 Size: 858 18 BspHI T'CATG_A Cuts at: 0 858 876 Size: 858 18 BsrDI GCAATG_nn' Cuts at: 0 98 876 Size: 98 778 BsrFI r'CCGG_y Cuts at: 0 259 288 633 876 Size: 259 29 345 243 Fragments arranged by size: 345 259 243 29 BssSI C'ACGA_G Cuts at: 0 523 876 Size: 523 353 BstYI r'GATC_y Cuts at: 0 210 876 Size: 210 666 BtgI C'CryG_G Cuts at: 0 74 299 387 419 644 876 Size: 74 225 88 32 225 232 Fragments arranged by size: 232 225 225 88 74 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 429 876 Size: 429 447 BtsI GCAGTG_nn' Cuts at: 0 783 876 Size: 783 93 EaeI y'GGCC_r Cuts at: 0 77 422 527 641 876 Size: 77 345 105 114 235 Fragments arranged by size: 345 235 114 105 77 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 163 508 876 Size: 163 345 368 Fragments arranged by size: 368 345 163 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 451 572 583 848 876 Size: 259 192 121 11 265 28 Fragments arranged by size: 265 259 192 121 28 11 EcoO109I rG'GnC_Cy Cuts at: 0 43 876 Size: 43 833 HaeII r_GCGC'y Cuts at: 0 501 876 Size: 501 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 131 163 506 538 832 864 876 Size: 131 32 343 32 294 32 12 Fragments arranged by size: 343 294 131 32 32 32 12 HincII GTy'rAC Cuts at: 0 234 840 876 Size: 234 606 36 Fragments arranged by size: 606 234 36 HindIII A'AGCT_T Cuts at: 0 200 876 Size: 200 676 MscI TGG'CCA Cuts at: 0 79 424 529 643 876 Size: 79 345 105 114 233 Fragments arranged by size: 345 233 114 105 79 MslI CAynn'nnrTG Cuts at: 0 776 876 Size: 776 100 MspA1I CmG'CkG Cuts at: 0 126 876 Size: 126 750 NaeI GCC'GGC Cuts at: 0 261 876 Size: 261 615 NcoI C'CATG_G Cuts at: 0 74 299 419 644 876 Size: 74 225 120 225 232 Fragments arranged by size: 232 225 225 120 74 NgoMIV G'CCGG_C Cuts at: 0 259 876 Size: 259 617 NspI r_CATG'y Cuts at: 0 279 876 Size: 279 597 PasI CC'CwG_GG Cuts at: 0 604 876 Size: 604 272 PflMI CCAn_nnn'nTGG Cuts at: 0 305 650 876 Size: 305 345 226 Fragments arranged by size: 345 305 226 PstI C_TGCA'G Cuts at: 0 366 876 Size: 366 510 SfcI C'TryA_G Cuts at: 0 362 876 Size: 362 514 SmlI C'TyrA_G Cuts at: 0 54 217 876 Size: 54 163 659 Fragments arranged by size: 659 163 54 StyI C'CwwG_G Cuts at: 0 74 299 419 644 876 Size: 74 225 120 225 232 Fragments arranged by size: 232 225 225 120 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 605 876 Size: 605 271 TatI w'GTAC_w Cuts at: 0 617 876 Size: 617 259 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 372 404 876 Size: 372 32 472 Fragments arranged by size: 472 372 32 Tth111I GACn'n_nGTC Cuts at: 0 865 876 Size: 865 11 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 426 876 Size: 426 450 XmnI GAAnn'nnTTC Cuts at: 0 169 212 514 876 Size: 169 43 302 362 Fragments arranged by size: 362 302 169 43 Enzymes that do cut: AccI AcuI AfeI AhdI ApoI AvaI BanI BbsI BcgI BglI BglII BmrI BplI BpmI BpuEI BsaI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BssSI BstYI BtgI BtgZI BtsI EaeI EciI Eco57MI EcoO109I HaeII Hin4I HincII HindIII MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PstI SfcI SmlI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI