################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AccIII AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI AspI AviII AvrII BbeI BciVI BclI BfrI BlnI BsaWI BseAI BsiWI Bsp1407I BspEI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CpoI DraI DraIII DrdI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1653 GACGTC GACGT^C Acc65I 1766 GGTACC G^GTACC AccI 1315 GTCTAC GT^[AC][GT]AC AcyI 1653 GACGTC G[GA]^CG[CT]C AflIII 1820 ACATGT A^C[GA][CT]GT AocI 2227 CCTGAGG CC^T[ACGT]AGG ApaI 2322 GGGCCC GGGCC^C Asp718I 1766 GGTACC G^GTACC BalI 248 TGGCCA TGG^CCA BbrPI 2169 CACGTG CAC^GTG BglI 1673 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1493 AGATCT A^GATCT BlpI 1668 GCTGAGC GC^T[ACGT]AGC BpmI 690 CTGGAG CTGGAG(16/14) Bpu1102I 1668 GCTGAGC GC^T[ACGT]AGC BsaAI 2169 CACGTG [CT]AC^GT[GA] BsaBI 1494 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1653 GACGTC G[GA]^CG[CT]C BsmI 477 GCATTC GAATGC(1/-1) BspDI 1891 ATCGAT AT^CGAT BspHI 2057 TCATGA T^CATGA BspLU11I 1820 ACATGT A^CATGT BstAPI 1218 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1755 GGTGACC G^GT[ACGT]ACC BstPI 1755 GGTGACC G^GT[ACGT]ACC BstXI 1808 CCACGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2227 CCTGAGG CC^T[ACGT]AGG CelII 1668 GCTGAGC GC^T[ACGT]AGC ClaI 1891 ATCGAT AT^CGAT Eco81I 2227 CCTGAGG CC^T[ACGT]AGG EcoO65I 1755 GGTGACC G^GT[ACGT]ACC EcoT14I 1796 CCAAGG C^C[AT][AT]GG HgaI 1262 GCGTC GACGC(5/10) Hin1I 1653 GACGTC G[GA]^CG[CT]C KpnI 1766 GGTACC GGTAC^C MamI 1494 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 248 TGGCCA TGG^CCA MscI 248 TGGCCA TGG^CCA MseI 103 TTAA T^TAA PmaCI 2169 CACGTG CAC^GTG PmlI 2169 CACGTG CAC^GTG PstI 1246 CTGCAG CTGCA^G RcaI 2057 TCATGA T^CATGA SbfI 1245 CCTGCAGG CCTGCA^GG SphI 360 GCATGC GCATG^C Sse8387I 1245 CCTGCAGG CCTGCA^GG StyI 1796 CCAAGG C^C[AT][AT]GG Tru9I 103 TTAA T^TAA XcmI 1593 CCAGCTGCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 44 AAATTC [GA]^AATT[CT] 108 AAATTC AhdI 781 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1819 GACATGTGGTC Aor51HI 2018 AGCGCT AGC^GCT 2098 AGCGCT ApoI 44 AAATTC [GA]^AATT[CT] 108 AAATTC Asp700I 43 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2061 GAACAAGTTC AspEI 781 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1819 GACATGTGGTC BamHI 1631 GGATCC G^GATCC 1723 GGATCC BbvCI 1407 GCTGAGG CCTCAGC(-5/-2) 2101 GCTGAGG BfaI 365 CTAG C^TAG 1931 CTAG BsmBI 737 GAGACG CGTCTC(1/5) 2275 GAGACG Eam1105I 781 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1819 GACATGTGGTC Eco47III 2018 AGCGCT AGC^GCT 2098 AGCGCT KspI 715 CCGCGG CCGC^GG 2318 CCGCGG MaeI 365 CTAG C^TAG 1931 CTAG MslI 1500 CATCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1809 CACGGCCGTG NaeI 1239 GCCGGC GCC^GGC 1678 GCCGGC NgoMIV 1239 GCCGGC G^CCGGC 1678 GCCGGC NspI 360 GCATGC [GA]CATG^[CT] 1820 ACATGT PflMI 683 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1062 CCACCTCTTGG PpuMI 779 GGGACCT [GA]G^G[AT]CC[CT] 1924 GGGACCC PvuII 1594 CAGCTG CAG^CTG 2251 CAGCTG SacI 1210 GAGCTC GAGCT^C 1960 GAGCTC SacII 715 CCGCGG CCGC^GG 2318 CCGCGG SmaI 1876 CCCGGG CCC^GGG 2083 CCCGGG StuI 1736 AGGCCT AGG^CCT 2235 AGGCCT Van91I 683 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1062 CCACCTCTTGG XmaCI 1876 CCCGGG C^CCGGG 2083 CCCGGG XmaI 1876 CCCGGG C^CCGGG 2083 CCCGGG XmnI 43 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2061 GAACAAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BmyI BpuAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII