(Linear) (Six-base) MAPSORT of: KIEE2092.seq Check: 2238 from: 1 to: 2346 KIEE2092.orf With 182 enzymes: SgfI * March 22, 2012 15:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1625 2346 Size: 1625 721 AatII G_ACGT'C Cuts at: 0 1657 2346 Size: 1657 689 AccI GT'mk_AC Cuts at: 0 1316 2346 Size: 1316 1030 Acc65I G'GTAC_C Cuts at: 0 1766 2346 Size: 1766 580 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 1387 1753 1971 2290 2316 2346 Size: 34 1353 366 218 319 26 30 Fragments arranged by size: 1353 366 319 218 34 30 26 AfeI AGC'GCT Cuts at: 0 2020 2100 2346 Size: 2020 80 246 Fragments arranged by size: 2020 246 80 AflIII A'CryG_T Cuts at: 0 1820 2346 Size: 1820 526 AhdI GACnn_n'nnGTC Cuts at: 0 786 1824 2346 Size: 786 1038 522 Fragments arranged by size: 1038 786 522 AleI CACnn'nnGTG Cuts at: 0 1813 2346 Size: 1813 533 AlwNI CAG_nnn'CTG Cuts at: 0 689 868 1224 1281 1599 2346 Size: 689 179 356 57 318 747 Fragments arranged by size: 747 689 356 318 179 57 ApaI G_GGCC'C Cuts at: 0 2326 2346 Size: 2326 20 ApoI r'AATT_y Cuts at: 0 44 108 2346 Size: 44 64 2238 Fragments arranged by size: 2238 64 44 AvaI C'yCGr_G Cuts at: 0 1876 2083 2217 2346 Size: 1876 207 134 129 Fragments arranged by size: 1876 207 134 129 BamHI G'GATC_C Cuts at: 0 1631 1723 2346 Size: 1631 92 623 Fragments arranged by size: 1631 623 92 BanI G'GyrC_C Cuts at: 0 1300 1766 1792 2209 2346 Size: 1300 466 26 417 137 Fragments arranged by size: 1300 466 417 137 26 BanII G_rGCy'C Cuts at: 0 1214 1964 2031 2326 2346 Size: 1214 750 67 295 20 Fragments arranged by size: 1214 750 295 67 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 215 227 829 1585 2346 Size: 215 12 602 756 761 Fragments arranged by size: 761 756 602 215 12 BbvCI CC'TCA_GC Cuts at: 0 1408 2102 2346 Size: 1408 694 244 Fragments arranged by size: 1408 694 244 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 256 290 379 413 1174 1208 2004 2038 Size: 256 34 89 34 761 34 796 34 Cuts at: 2038 2346 Size: 308 Fragments arranged by size: 796 761 308 256 89 34 34 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1679 2346 Size: 1679 667 BglII A'GATC_T Cuts at: 0 1493 2346 Size: 1493 853 BlpI GC'TnA_GC Cuts at: 0 1669 2346 Size: 1669 677 Bme1580I G_kGCm'C Cuts at: 0 1184 1303 2212 2326 2346 Size: 1184 119 909 114 20 Fragments arranged by size: 1184 909 119 114 20 BmgBI CAC'GTC Cuts at: 0 1403 2346 Size: 1403 943 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 711 2346 Size: 711 1635 Bpu10I CC'TnA_GC Cuts at: 0 861 1408 2102 2346 Size: 861 547 694 244 Fragments arranged by size: 861 694 547 244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 1198 1414 1513 1633 1672 2098 2323 Size: 163 1035 216 99 120 39 426 225 Cuts at: 2323 2346 Size: 23 Fragments arranged by size: 1035 426 225 216 163 120 99 39 23 BsaI GGTCTCn'nnnn_ Cuts at: 0 961 1537 2128 2346 Size: 961 576 591 218 Fragments arranged by size: 961 591 576 218 BsaAI yAC'GTr Cuts at: 0 2171 2346 Size: 2171 175 BsaBI GATnn'nnATC Cuts at: 0 1498 2346 Size: 1498 848 BsaHI Gr'CG_yC Cuts at: 0 1654 2346 Size: 1654 692 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 903 933 1261 1291 2346 Size: 903 30 328 30 1055 Fragments arranged by size: 1055 903 328 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 160 242 610 895 898 901 910 Size: 94 66 82 368 285 3 3 9 Cuts at: 910 913 916 946 949 952 955 970 973 Size: 3 3 30 3 3 3 15 3 Cuts at: 973 976 979 1000 1282 1309 2103 2346 Size: 3 3 21 282 27 794 243 Fragments arranged by size: 794 368 285 282 243 94 82 66 30 27 21 15 9 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 263 1436 1965 2253 2346 Size: 263 1173 529 288 93 Fragments arranged by size: 1173 529 288 263 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 620 1603 1621 2346 Size: 620 983 18 725 Fragments arranged by size: 983 725 620 18 BsiEI CG_ry'CG Cuts at: 0 712 744 836 1095 1751 1814 2346 Size: 712 32 92 259 656 63 532 Fragments arranged by size: 712 656 532 259 92 63 32 BsiHKAI G_wGCw'C Cuts at: 0 392 916 1214 1523 1964 1997 2346 Size: 392 524 298 309 441 33 349 Fragments arranged by size: 524 441 392 349 309 298 33 BsmI GAATG_Cn' Cuts at: 0 477 2346 Size: 477 1869 BsmBI CGTCTCn'nnnn_ Cuts at: 0 731 2269 2346 Size: 731 1538 77 Fragments arranged by size: 1538 731 77 Bsp1286I G_dGCh'C Cuts at: 0 392 916 1184 1214 1303 1523 1964 1997 Size: 392 524 268 30 89 220 441 33 Cuts at: 1997 2031 2212 2326 2346 Size: 34 181 114 20 Fragments arranged by size: 524 441 392 268 220 181 114 89 34 33 30 20 BspHI T'CATG_A Cuts at: 0 2057 2346 Size: 2057 289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 791 1244 1625 1670 2346 Size: 791 453 381 45 676 Fragments arranged by size: 791 676 453 381 45 BsrBI CCG'CTC Cuts at: 0 528 585 600 612 723 2346 Size: 528 57 15 12 111 1623 Fragments arranged by size: 1623 528 111 57 15 12 BsrFI r'CCGG_y Cuts at: 0 970 1239 1678 2346 Size: 970 269 439 668 Fragments arranged by size: 970 668 439 269 BstAPI GCAn_nnn'nTGC Cuts at: 0 1224 2346 Size: 1224 1122 BstEII G'GTnAC_C Cuts at: 0 1755 2346 Size: 1755 591 BstXI CCAn_nnnn'nTGG Cuts at: 0 1815 2346 Size: 1815 531 BstYI r'GATC_y Cuts at: 0 1493 1631 1723 1917 2346 Size: 1493 138 92 194 429 Fragments arranged by size: 1493 429 194 138 92 Bsu36I CC'TnA_GG Cuts at: 0 2228 2346 Size: 2228 118 BtgI C'CryG_G Cuts at: 0 715 1808 1814 2318 2346 Size: 715 1093 6 504 28 Fragments arranged by size: 1093 715 504 28 6 ClaI AT'CG_AT Cuts at: 0 1892 2346 Size: 1892 454 EaeI y'GGCC_r Cuts at: 0 248 709 741 833 1811 2346 Size: 248 461 32 92 978 535 Fragments arranged by size: 978 535 461 248 92 32 EagI C'GGCC_G Cuts at: 0 709 741 833 1811 2346 Size: 709 32 92 978 535 Fragments arranged by size: 978 709 535 92 32 EarI CTCTTCn'nnn_ Cuts at: 0 198 448 755 872 887 941 947 2346 Size: 198 250 307 117 15 54 6 1399 Fragments arranged by size: 1399 307 250 198 117 54 15 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 607 2346 Size: 607 1739 EcoICRI GAG'CTC Cuts at: 0 1212 1962 2346 Size: 1212 750 384 Fragments arranged by size: 1212 750 384 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 711 1387 1753 1971 2290 2316 2346 Size: 34 677 676 366 218 319 26 30 Fragments arranged by size: 677 676 366 319 218 34 30 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 145 1165 2231 2346 Size: 145 1020 1066 115 Fragments arranged by size: 1066 1020 145 115 EcoO109I rG'GnC_Cy Cuts at: 0 780 1925 2222 2309 2323 2346 Size: 780 1145 297 87 14 23 Fragments arranged by size: 1145 780 297 87 23 14 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1407 2001 2346 Size: 1407 594 345 Fragments arranged by size: 1407 594 345 HaeII r_GCGC'y Cuts at: 0 1160 2022 2102 2346 Size: 1160 862 80 244 Fragments arranged by size: 1160 862 244 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 772 804 829 861 976 1008 1708 1740 Size: 772 32 25 32 115 32 700 32 Cuts at: 1740 1810 1842 2346 Size: 70 32 504 Fragments arranged by size: 772 700 504 115 70 32 32 32 32 32 25 KpnI G_GTAC'C Cuts at: 0 1770 2346 Size: 1770 576 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 379 815 1682 2346 Size: 379 436 867 664 Fragments arranged by size: 867 664 436 379 MscI TGG'CCA Cuts at: 0 250 2346 Size: 250 2096 MslI CAynn'nnrTG Cuts at: 0 1504 1813 2346 Size: 1504 309 533 Fragments arranged by size: 1504 533 309 MspA1I CmG'CkG Cuts at: 0 564 717 850 1596 2253 2320 2346 Size: 564 153 133 746 657 67 26 Fragments arranged by size: 746 657 564 153 133 67 26 NaeI GCC'GGC Cuts at: 0 1241 1680 2346 Size: 1241 439 666 Fragments arranged by size: 1241 666 439 NgoMIV G'CCGG_C Cuts at: 0 1239 1678 2346 Size: 1239 439 668 Fragments arranged by size: 1239 668 439 NspI r_CATG'y Cuts at: 0 364 1824 2346 Size: 364 1460 522 Fragments arranged by size: 1460 522 364 PciI A'CATG_T Cuts at: 0 1820 2346 Size: 1820 526 PflMI CCAn_nnn'nTGG Cuts at: 0 689 1068 2346 Size: 689 379 1278 Fragments arranged by size: 1278 689 379 PfoI T'CCnGG_A Cuts at: 0 481 2346 Size: 481 1865 PmlI CAC'GTG Cuts at: 0 2171 2346 Size: 2171 175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1072 1104 2346 Size: 1072 32 1242 Fragments arranged by size: 1242 1072 32 PpuMI rG'GwC_Cy Cuts at: 0 780 1925 2346 Size: 780 1145 421 Fragments arranged by size: 1145 780 421 PspOMI G'GGCC_C Cuts at: 0 2322 2346 Size: 2322 24 PstI C_TGCA'G Cuts at: 0 1250 2346 Size: 1250 1096 PvuII CAG'CTG Cuts at: 0 1596 2253 2346 Size: 1596 657 93 Fragments arranged by size: 1596 657 93 SacI G_AGCT'C Cuts at: 0 1214 1964 2346 Size: 1214 750 382 Fragments arranged by size: 1214 750 382 SacII CC_GC'GG Cuts at: 0 718 2321 2346 Size: 718 1603 25 Fragments arranged by size: 1603 718 25 SanDI GG'GwC_CC Cuts at: 0 1925 2346 Size: 1925 421 SbfI CC_TGCA'GG Cuts at: 0 1250 2346 Size: 1250 1096 SfcI C'TryA_G Cuts at: 0 663 1246 1317 1953 2244 2346 Size: 663 583 71 636 291 102 Fragments arranged by size: 663 636 583 291 102 71 SmaI CCC'GGG Cuts at: 0 1878 2085 2346 Size: 1878 207 261 Fragments arranged by size: 1878 261 207 SmlI C'TyrA_G Cuts at: 0 142 1213 1429 1528 1648 1687 2113 2338 Size: 142 1071 216 99 120 39 426 225 Cuts at: 2338 2346 Size: 8 Fragments arranged by size: 1071 426 225 216 142 120 99 39 8 SphI G_CATG'C Cuts at: 0 364 2346 Size: 364 1982 StuI AGG'CCT Cuts at: 0 1738 2237 2346 Size: 1738 499 109 Fragments arranged by size: 1738 499 109 StyI C'CwwG_G Cuts at: 0 1796 2346 Size: 1796 550 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1109 1123 1684 2245 2346 Size: 1109 14 561 561 101 Fragments arranged by size: 1109 561 561 101 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 903 935 1261 1293 1917 1949 2346 Size: 903 32 326 32 624 32 397 Fragments arranged by size: 903 624 397 326 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1600 2346 Size: 1600 746 XmaI C'CCGG_G Cuts at: 0 1876 2083 2346 Size: 1876 207 263 Fragments arranged by size: 1876 263 207 XmnI GAAnn'nnTTC Cuts at: 0 47 2065 2346 Size: 47 2018 281 Fragments arranged by size: 2018 281 47 ZraI GAC'GTC Cuts at: 0 1655 2346 Size: 1655 691 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI NgoMIV NspI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SbfI SfcI SmaI SmlI SphI StuI StyI TaqII TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AclI AflII AgeI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BmrI BmtI BplI BsaWI BsiWI BspEI BsrDI BsrGI BssHII BssSI BstBI BstZ17I BtgZI BtsI CspCI DraI DraIII DrdI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI