Restriction map for
pFN21AE0992
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AgeI AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI AspEI AvrII BamHI BbeI
BbrPI BbvCI BfrI BglI BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstUI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FseI HaeII HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SphI Sse8387I SwaI Van91I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 86 enzymes) #
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Acc65I 1025 GGTACC G^GTACC
AccI 398 GTAGAC GT^[AC][GT]AC
AcyI 1767 GGCGTC G[GA]^CG[CT]C
Alw44I 1293 GTGCAC G^TGCAC
ApaLI 1293 GTGCAC G^TGCAC
Asp700I 1823 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1025 GGTACC G^GTACC
AspHI 1293 GTGCAC G[AT]GC[AT]^C
AspI 1370 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1736 CTCGAG C^[CT]CG[GA]G
AvaII 297 GGACC G^G[AT]CC
AviII 1847 TGCGCA TGC^GCA
BalI 1529 TGGCCA TGG^CCA
BanI 1025 GGTACC G^G[CT][GA]CC
BanII 1169 GAGCCC G[GA]GC[CT]^C
BbsI 57 GAAGAC GAAGAC(2/6)
BcgI 1268 CGAAGTTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 231 GTATCC GTATCC(6/5)
BclI 1548 TGATCA T^GATCA
BcnI 421 CCCGG CC^[GC]GG
BlpI 1398 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1398 GCTGAGC GC^T[ACGT]AGC
BpuAI 57 GAAGAC GAAGAC(2/6)
BsaAI 697 TACGTA [CT]AC^GT[GA]
BsaHI 1767 GGCGTC G[GA]^CG[CT]C
BsaJI 1727 CCATGG C^C[ACGT][ACGT]GG
BsgI 1276 GTGCAG GTGCAG(16/14)
BsiHKAI 1293 GTGCAC G[AT]GC[AT]^C
BsmBI 1388 GAGACG CGTCTC(1/5)
BsmFI 296 GGGAC GGGAC(10/14)
BsoBI 1736 CTCGAG C^[CT]CG[GA]G
Bsp1407I 839 TGTACA T^GTACA
BspHI 249 TCATGA T^CATGA
BsrDI 1210 GCAATG GCAATG(2/0)
BsrGI 839 TGTACA T^GTACA
BstBI 1834 TTCGAA TT^CGAA
BstEII 1722 GGTAACC G^GT[ACGT]ACC
BstPI 1722 GGTAACC G^GT[ACGT]ACC
Cac8I 1167 GCGAGC GC[ACGT]^[ACGT]GC
CelII 1398 GCTGAGC GC^T[ACGT]AGC
CfoI 1848 GCGC GCG^C
Cfr13I 297 GGACC G^G[ACGT]CC
DraII 296 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1727 CCATGG C^C[GA][CT]GG
EaeI 1529 TGGCCA [CT]^GGCC[GA]
EcoNI 1112 CCTTTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 296 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1722 GGTAACC G^GT[ACGT]ACC
EcoT14I 1727 CCATGG C^C[AT][AT]GG
FauI 1498 GCGGG CCCGC(4/6)
FbaI 1548 TGATCA T^GATCA
FspI 1847 TGCGCA TGC^GCA
HapII 422 CCGG C^CGG
HgaI 1768 GCGTC GACGC(5/10)
HhaI 1848 GCGC GCG^C
Hin1I 1767 GGCGTC G[GA]^CG[CT]C
HinP1I 1848 GCGC G^CGC
HincII 827 GTCAAC GT[CT]^[GA]AC
HindII 827 GTCAAC GT[CT]^[GA]AC
HpaII 422 CCGG C^CGG
KpnI 1025 GGTACC GGTAC^C
MluNI 1529 TGGCCA TGG^CCA
MscI 1529 TGGCCA TGG^CCA
MslI 419 CACCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 422 CCGG C^CGG
NciI 421 CCCGG CC^[GC]GG
NcoI 1727 CCATGG C^CATGG
NspV 1834 TTCGAA TT^CGAA
PaeR7I 1736 CTCGAG C^TCGAG
PflFI 1370 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1748 GACTC GAGTC(4/5)
PpuMI 296 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1064 CTGCAG CTGCA^G
RcaI 249 TCATGA T^CATGA
Sau96I 297 GGACC G^G[ACGT]CC
SfcI 1064 CTGCAG C^T[GA][CT]AG
SfuI 1834 TTCGAA TT^CGAA
SmlI 1736 CTCGAG C^T[CT][GA]AG
SnaBI 697 TACGTA TAC^GTA
SpeI 1863 ACTAGT A^CTAGT
SspBI 839 TGTACA T^GTACA
SspI 343 AATATT AAT^ATT
StyI 1727 CCATGG C^C[AT][AT]GG
Tth111I 1370 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1736 CTCGAG C^TCGAG
XmnI 1823 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BglII 1464 AGATCT A^GATCT
1839 AGATCT
BmyI 1169 GAGCCC G[AGT]GC[ACT]^C
1293 GTGCAC
BpmI 473 CTCCAG CTGGAG(16/14)
1438 CTGGAG
BsiYI 1016 CCAGGCTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1112 CCTTTATGAGG
BslI 1016 CCAGGCTTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1112 CCTTTATGAGG
BsmI 1050 GAATGC GAATGC(1/-1)
1688 GAATGC
Bsp1286I 1169 GAGCCC G[AGT]GC[ACT]^C
1293 GTGCAC
BstYI 1464 AGATCT [GA]^GATC[CT]
1839 AGATCT
MaeII 698 ACGT A^CGT
1391 ACGT
MflI 1464 AGATCT [GA]^GATC[CT]
1839 AGATCT
NspI 951 ACATGC [GA]CATG^[CT]
1403 GCATGT
SfaNI 253 GATGC GCATC(5/9)
1791 GATGC
StuI 499 AGGCCT AGG^CCT
1825 AGGCCT
Tsp45I 42 GTCAC ^GT[GC]AC
1012 GTCAC
XcmI 1532 CCAGGTATGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1709 CCAATTATGAAGTGG
XhoII 1464 AGATCT [GA]^GATC[CT]
1839 AGATCT
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BfaI BseRI BsmAI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I