Restriction map for
pFN21AE0992
(Circular) (Six-base) MAPSORT of: pFN21AE0992.seq Check: 4802 from: 1 to: 6598
pFN21AE0992 6598 bp
With 182 enzymes: SgfI *
March 28, 2012 12:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4408 278
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2404 3941 1937
Size: 467 1537 4594
Fragments arranged by size:
4594 1537 467
Acc65I G'GTAC_C
Cuts at: 3030 3919 3030
Size: 889 5709
AclI AA'CG_TT
Cuts at: 1563 4725 5098 1563
Size: 3162 373 3063
Fragments arranged by size:
3162 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3090 3613 3627 4661 6045 6313 1632
Size: 1458 523 14 1034 1384 268 1917
Fragments arranged by size:
1917 1458 1384 1034 523 268 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6401
AflIII A'CryG_T
Cuts at: 1203 5497 6474 1203
Size: 4294 977 1327
Fragments arranged by size:
4294 1327 977
AgeI A'CCGG_T
Cuts at: 5429 5429
Size: 6598
AhdI GACnn_n'nnGTC
Cuts at: 5327 5327
Size: 6598
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6598
AlwNI CAG_nnn'CTG
Cuts at: 4341 5913 4341
Size: 1572 5026
ApaLI G'TGCA_C
Cuts at: 3298 4655 5811 3298
Size: 1357 1156 4085
Fragments arranged by size:
4085 1357 1156
ApoI r'AATT_y
Cuts at: 1493 2008 2372 3358 3907 4133 4285 6394 1493
Size: 515 364 986 549 226 152 2109 1697
Fragments arranged by size:
2109 1697 986 549 515 364 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5152 160
Size: 872 4120 1606
Fragments arranged by size:
4120 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
AvaI C'yCGr_G
Cuts at: 1870 1943 3741 3923 1870
Size: 73 1798 182 4545
Fragments arranged by size:
4545 1798 182 73
BamHI G'GATC_C
Cuts at: 3928 4295 3928
Size: 367 6231
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3030 3919 5374 618
Size: 325 217 627 1243 889 1455 1842
Fragments arranged by size:
1842 1455 1243 889 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3178 3917 1391
Size: 1787 739 4072
Fragments arranged by size:
4072 1787 739
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2069 928
Size: 971 170 5457
Fragments arranged by size:
5457 971 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3262 3296 4789 4823 1903
Size: 34 1325 34 1493 34 3678
Fragments arranged by size:
3678 1493 1325 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2247 4490 5700 2247
Size: 2243 1210 3145
Fragments arranged by size:
3145 2243 1210
BclI T'GATC_A
Cuts at: 1548 3553 1548
Size: 2005 4593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5209 136
Size: 107 122 71 631 605 3537 1525
Fragments arranged by size:
3537 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3469 3844 6593 3469
Size: 375 2749 3474
Fragments arranged by size:
3474 2749 375
BlpI GC'TnA_GC
Cuts at: 3404 4015 3404
Size: 611 5987
Bme1580I G_kGCm'C
Cuts at: 1790 3302 4659 5815 1790
Size: 1512 1357 1156 2573
Fragments arranged by size:
2573 1512 1357 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6598
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3029 3172 4329
Size: 416 53 83 399 24 1612 143 1157
Cuts at: 4329 5287 442
Size: 958 1753
Fragments arranged by size:
1753 1612 1157 958 416 399 143 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2463 3464 5258 1239
Size: 1224 1001 1794 2579
Fragments arranged by size:
2579 1794 1224 1001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4723 5588 5886 4723
Size: 865 298 5435
Fragments arranged by size:
5435 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5261 882
Size: 617 3762 2219
Fragments arranged by size:
3762 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2704 4389 493
Size: 711 1500 1685 2702
Fragments arranged by size:
2702 1685 1500 711
BsaBI GATnn'nnATC
Cuts at: 6592 6592
Size: 6598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3773 4405 4787 5422
Size: 53 83 186 936 2240 632 382 635
Cuts at: 5422 275
Size: 1451
Fragments arranged by size:
2240 1451 936 635 632 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5031 5429 5703 5850 6261 5031
Size: 398 274 147 411 5368
Fragments arranged by size:
5368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2837 2867 2911 2941 1178
Size: 30 165 30 1434 30 44 30 4835
Fragments arranged by size:
4835 1434 165 44 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2774 2889 3239 1186
Size: 856 732 115 350 4545
Fragments arranged by size:
4545 856 732 350 115
BseYI C'CCAG_C
Cuts at: 3303 5801 6297 3303
Size: 2498 496 3604
Fragments arranged by size:
3604 2498 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3302 1163
Size: 573 1566 4459
Fragments arranged by size:
4459 1566 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4048 4809 5837 1540
Size: 275 186 2047 761 1028 2301
Fragments arranged by size:
2301 2047 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3302 4659 4744 5815 3302
Size: 1357 85 1071 4085
Fragments arranged by size:
4085 1357 1071 85
BsmI GAATG_Cn'
Cuts at: 3061 3699 4109 4202 3061
Size: 638 410 93 5457
Fragments arranged by size:
5457 638 410 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3387 3387
Size: 6598
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3178 3302 3917 4659 4744 5815 1391
Size: 399 1388 124 615 742 85 1071 2174
Fragments arranged by size:
2174 1388 1071 742 615 399 124 85
BspEI T'CCGG_A
Cuts at: 6261 6261
Size: 6598
BspHI T'CATG_A
Cuts at: 2254 4487 6217 2254
Size: 2233 1730 2635
Fragments arranged by size:
2635 2233 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3953 844
Size: 3109 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4485 1394
Size: 429 2662 3507
Fragments arranged by size:
3507 2662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3222 5093 5267 65
Size: 1183 1974 1871 174 1396
Fragments arranged by size:
1974 1871 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5242 5429 1700
Size: 110 3432 187 2869
Fragments arranged by size:
3432 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2844 96
Size: 2748 3850
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6598
BssSI C'ACGA_G
Cuts at: 4658 5670 4658
Size: 1012 5586
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6598
BstBI TT'CG_AA
Cuts at: 3840 5402 3840
Size: 1562 5036
BstEII G'GTnAC_C
Cuts at: 1184 3727 1184
Size: 2543 4055
BstYI r'GATC_y
Cuts at: 1972 3469 3844 3928 4295 4680 4697 6149 6235
Size: 1497 375 84 367 385 17 1452 86
Cuts at: 6235 6247 6593 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1497 1452 385 375 367 346 86
84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3732 513
Size: 552 2667 3379
Fragments arranged by size:
3379 2667 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2052 487
Size: 683 56 826 5033
Fragments arranged by size:
5033 826 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 3066 4114 4908 4928 2797
Size: 269 1048 794 20 4467
Fragments arranged by size:
4467 1048 794 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6563
DraI TTT'AAA
Cuts at: 3902 3902
Size: 6598
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5605 809
Size: 507 4289 1802
Fragments arranged by size:
4289 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3534 4045 4934
Size: 54 1425 50 275 18 1704 511 889
Cuts at: 4934 6563 8
Size: 1629 43
Fragments arranged by size:
1704 1629 1425 889 511 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4045 1537
Size: 275 2233 4090
Fragments arranged by size:
4090 2233 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3889 4528 1353
Size: 2536 639 3423
Fragments arranged by size:
3423 2536 639
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5185 5559 5705 387
Size: 1064 138 3596 374 146 1280
Fragments arranged by size:
3596 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2463 3090 3464 3613 3627 4661 5258
Size: 393 831 627 374 149 14 1034 597
Cuts at: 5258 6045 6313 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1034 831 787 627 597 393 374
268 149 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3121 3121
Size: 6598
EcoO109I rG'GnC_Cy
Cuts at: 2302 2302
Size: 6598
EcoRI G'AATT_C
Cuts at: 3907 4285 3907
Size: 378 6220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2108 3852 2108
Size: 1744 4854
FspI TGC'GCA
Cuts at: 3854 5104 3854
Size: 1250 5348
HaeII r_GCGC'y
Cuts at: 1678 5745 1678
Size: 4067 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2347 2379 2580 2612 3364
Size: 32 135 32 775 32 201 32 752
Cuts at: 3364 3396 3397 3424 3429 3456 3635 3667 5239
Size: 32 1 27 5 27 179 32 1572
Cuts at: 5239 5271 5313 5345 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1572 775 752 201 179 135 42
32 32 32 32 32 32 32 32
27 27 5 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2834 3942 4188 669
Size: 988 281 896 1108 246 3079
Fragments arranged by size:
3079 1108 988 896 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6598
HpaI GTT'AAC
Cuts at: 4188 4188
Size: 6598
KpnI G_GTAC'C
Cuts at: 3034 3923 3034
Size: 889 5709
MfeI C'AATT_G
Cuts at: 4197 4197
Size: 6598
MluI A'CGCG_T
Cuts at: 6474 6474
Size: 6598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3391 3801 5712 5896 1305
Size: 2086 410 1911 184 2007
Fragments arranged by size:
2086 2007 1911 410 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3536 10
Size: 54 1425 343 1704 3072
Fragments arranged by size:
3072 1704 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2428 4556 4915 5074 518
Size: 648 1262 2128 359 159 2042
Fragments arranged by size:
2128 2042 1262 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4014 4691 5839 6084 1606
Size: 2408 677 1148 245 2120
Fragments arranged by size:
2408 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3732 513
Size: 552 2667 3379
Fragments arranged by size:
3379 2667 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6598
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6598
NotI GC'GGCC_GC
Cuts at: 4045 4045
Size: 6598
NspI r_CATG'y
Cuts at: 1132 1229 2960 3412 3956 5501 1132
Size: 97 1731 452 544 1545 2229
Fragments arranged by size:
2229 1731 1545 544 452 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6598
PciI A'CATG_T
Cuts at: 5497 5497
Size: 6598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6598
PmeI GTTT'AAAC
Cuts at: 3902 3902
Size: 6598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 6180 6212 4667
Size: 32 1481 32 5053
Fragments arranged by size:
5053 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 2302
Size: 6598
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6598
PsiI TTA'TAA
Cuts at: 4168 4168
Size: 6598
PspXI vC'TCGA_Gb
Cuts at: 3741 3741
Size: 6598
PstI C_TGCA'G
Cuts at: 830 3073 3950 830
Size: 2243 877 3478
Fragments arranged by size:
3478 2243 877
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6598
SalI G'TCGA_C
Cuts at: 1936 3940 1936
Size: 2004 4594
SbfI CC_TGCA'GG
Cuts at: 3950 3950
Size: 6598
ScaI AGT'ACT
Cuts at: 4846 6558 4846
Size: 1712 4886
SfcI C'TryA_G
Cuts at: 826 1045 3069 3946 5081 5762 5953 826
Size: 219 2024 877 1135 681 191 1471
Fragments arranged by size:
2024 1471 1135 877 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6598
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6598
SmaI CCC'GGG
Cuts at: 1872 3925 1872
Size: 2053 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3741 4702 5603 5865 820
Size: 197 926 1798 961 901 262 1553
Fragments arranged by size:
1798 1553 961 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2704 4389 493
Size: 2211 1685 2702
Fragments arranged by size:
2702 2211 1685
SpeI A'CTAG_T
Cuts at: 152 3868 152
Size: 3716 2882
SphI G_CATG'C
Cuts at: 1132 3956 1132
Size: 2824 3774
SspI AAT'ATT
Cuts at: 5 52 2350 4522 5
Size: 47 2298 2172 2081
Fragments arranged by size:
2298 2172 2081 47
StuI AGG'CCT
Cuts at: 1517 2506 3832 1517
Size: 989 1326 4283
Fragments arranged by size:
4283 1326 989
StyI C'CwwG_G
Cuts at: 513 1065 3732 4037 513
Size: 552 2667 305 3074
Fragments arranged by size:
3074 2667 552 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2299 3076 3317 4625 4642 4795 4980
Size: 1255 719 777 241 1308 17 153 185
Cuts at: 4980 5425 325
Size: 445 1498
Fragments arranged by size:
1498 1308 1255 777 719 445 241 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2098 2844 3180
Size: 274 80 33 51 1443 121 746 336
Cuts at: 3180 3558 4844 6556 96
Size: 378 1286 1712 138
Fragments arranged by size:
1712 1443 1286 746 378 336 274 138
121 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6558 6590 6558
Size: 32 6566
Tth111I GACn'n_nGTC
Cuts at: 1316 3378 1316
Size: 2062 4536
XbaI T'CTAG_A
Cuts at: 3934 3934
Size: 6598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3544 3721 3544
Size: 177 6421
XhoI C'TCGA_G
Cuts at: 1943 3741 1943
Size: 1798 4800
XmaI C'CCGG_G
Cuts at: 1870 3923 1870
Size: 2053 4545
XmnI GAAnn'nnTTC
Cuts at: 3832 4727 3832
Size: 895 5703
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4406 276
Size: 53 83 186 936 2872 2468
Fragments arranged by size:
2872 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI