################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbvCI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FbaI FseI FspI HincII HindII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 526 GACGTC GACGT^C AflIII 710 ACATGT A^C[GA][CT]GT AlwNI 562 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1021 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 362 GTGCTC G[AT]GC[AT]^C AvaI 365 CTCGAG C^[CT]CG[GA]G BanI 24 GGCGCC G^G[CT][GA]CC BanII 322 GAGCCC G[GA]GC[CT]^C BbeI 24 GGCGCC GGCGC^C BcgI 531 CGATACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1120 GGATAC GTATCC(6/5) BfaI 37 CTAG C^TAG BglII 1169 AGATCT A^GATCT BpmI 1037 CTGGAG CTGGAG(16/14) BsiHKAI 362 GTGCTC G[AT]GC[AT]^C BsiYI 1068 CCCAGGTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1068 CCCAGGTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 564 GGGAC GGGAC(10/14) BsoBI 365 CTCGAG C^[CT]CG[GA]G Bsp1407I 894 TGTACA T^GTACA BspDI 425 ATCGAT AT^CGAT BspHI 144 TCATGA T^CATGA BspLU11I 710 ACATGT A^CATGT BsrDI 166 CATTGC GCAATG(2/0) BsrGI 894 TGTACA T^GTACA ClaI 425 ATCGAT AT^CGAT DraI 1159 TTTAAA TTT^AAA DrdI 33 GACCCTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 8 CCATGG C^C[GA][CT]GG EaeI 5 CGGCCA [CT]^GGCC[GA] EcoRI 440 GAATTC G^AATTC EcoRV 186 GATATC GAT^ATC EcoT14I 8 CCATGG C^C[AT][AT]GG HaeII 24 GGCGCC [GA]GCGC^[CT] HapII 542 CCGG C^CGG HgaI 1391 GACGC GACGC(5/10) HindIII 409 AAGCTT A^AGCTT HpaII 542 CCGG C^CGG KasI 24 GGCGCC G^GCGCC MaeI 37 CTAG C^TAG MaeIII 1013 GTGAC ^GT[ACGT]AC MslI 621 CATAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 542 CCGG C^CGG NarI 24 GGCGCC GG^CGCC NcoI 8 CCATGG C^CATGG NdeI 1198 CATATG CA^TATG PaeR7I 365 CTCGAG C^TCGAG PstI 635 CTGCAG CTGCA^G RcaI 144 TCATGA T^CATGA SfoI 24 GGCGCC GGC^GCC SspBI 894 TGTACA T^GTACA SspI 314 AATATT AAT^ATT StyI 8 CCATGG C^C[AT][AT]GG Tsp45I 1013 GTGAC ^GT[GC]AC XhoI 365 CTCGAG C^TCGAG XmnI 1021 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AvaII 1033 GGACC G^G[AT]CC 1246 GGTCC BmyI 322 GAGCCC G[AGT]GC[ACT]^C 362 GTGCTC BsaI 13 GAGACC GGTCTC(1/5) 794 GAGACC BsmBI 19 GAGACG CGTCTC(1/5) 1356 GAGACG Bsp1286I 322 GAGCCC G[AGT]GC[ACT]^C 362 GTGCTC BstYI 93 GGATCT [GA]^GATC[CT] 1169 AGATCT Cac8I 131 GCCAGC GC[ACGT]^[ACGT]GC 407 GCAAGC CfoI 25 GCGC GCG^C 394 GCGC DraII 1032 AGGACCT [GA]G^G[ACGT]CC[CT] 1245 GGGTCCT EcoO109I 1032 AGGACCT [GA]G^G[ACGT]CC[CT] 1245 GGGTCCT HhaI 25 GCGC GCG^C 394 GCGC HinP1I 25 GCGC G^CGC 394 GCGC HphI 1012 GGTGA GGTGA(8/7) 1048 GGTGA MaeII 527 ACGT A^CGT 1016 ACGT MflI 93 GGATCT [GA]^GATC[CT] 1169 AGATCT NspI 710 ACATGT [GA]CATG^[CT] 1204 GCATGT PpuMI 1032 AGGACCT [GA]G^G[AT]CC[CT] 1245 GGGTCCT PvuII 536 CAGCTG CAG^CTG 698 CAGCTG SfaNI 382 GATGC GCATC(5/9) 949 GATGC SfcI 635 CTGCAG C^T[GA][CT]AG 1226 CTATAG SmlI 307 CTCAAG C^T[CT][GA]AG 365 CTCGAG StuI 293 AGGCCT AGG^CCT 965 AGGCCT XhoII 93 GGATCT [GA]^GATC[CT] 1169 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI BbsI BbvI BpuAI BsaHI BsaJI BseRI BsmAI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII Hin1I HinfI ItaI Ksp632I MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I