Restriction map for
pFN21AE3201
(Linear) (Six-base) MAPSORT of: KIEE3201.seq Check: 215 from: 1 to: 1413
KIEE3201.orf
With 182 enzymes: SgfI *
March 22, 2012 16:20 ..
AatII G_ACGT'C
Cuts at: 0 530 1413
Size: 530 883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1413
Size: 533 880
AflIII A'CryG_T
Cuts at: 0 710 1413
Size: 710 703
AlwNI CAG_nnn'CTG
Cuts at: 0 567 1413
Size: 567 846
ApoI r'AATT_y
Cuts at: 0 440 800 1051 1413
Size: 440 360 251 362
Fragments arranged by size:
440 362 360 251
AvaI C'yCGr_G
Cuts at: 0 365 1413
Size: 365 1048
BanI G'GyrC_C
Cuts at: 0 24 1413
Size: 24 1389
BanII G_rGCy'C
Cuts at: 0 326 1413
Size: 326 1087
BbeI G_GCGC'C
Cuts at: 0 28 1413
Size: 28 1385
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 168 380 1304 1413
Size: 129 39 212 924 109
Fragments arranged by size:
924 212 129 109 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 520 554 1413
Size: 520 34 859
Fragments arranged by size:
859 520 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1114 1413
Size: 1114 299
BglII A'GATC_T
Cuts at: 0 1169 1413
Size: 1169 244
BmgBI CAC'GTC
Cuts at: 0 1017 1413
Size: 1017 396
BmrI ACTGGGnnnn_n'
Cuts at: 0 851 1413
Size: 851 562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1058 1413
Size: 1058 355
Bpu10I CC'TnA_GC
Cuts at: 0 1007 1413
Size: 1007 406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1413
Size: 292 1121
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 788 1413
Size: 7 781 625
Fragments arranged by size:
781 625 7
BsaHI Gr'CG_yC
Cuts at: 0 25 527 1392 1413
Size: 25 502 865 21
Fragments arranged by size:
865 502 25 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 1413
Size: 313 30 1070
Fragments arranged by size:
1070 313 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 1291 1294 1297 1306 1366 1377 1413
Size: 55 1236 3 3 9 60 11 36
Fragments arranged by size:
1236 60 55 36 11 9 3 3
BsiHKAI G_wGCw'C
Cuts at: 0 366 1413
Size: 366 1047
BsmBI CGTCTCn'nnnn_
Cuts at: 0 13 1350 1413
Size: 13 1337 63
Fragments arranged by size:
1337 63 13
Bsp1286I G_dGCh'C
Cuts at: 0 326 366 1413
Size: 326 40 1047
Fragments arranged by size:
1047 326 40
BspHI T'CATG_A
Cuts at: 0 144 1413
Size: 144 1269
BsrDI GCAATG_nn'
Cuts at: 0 165 1413
Size: 165 1248
BsrGI T'GTAC_A
Cuts at: 0 894 1413
Size: 894 519
BstYI r'GATC_y
Cuts at: 0 93 1169 1413
Size: 93 1076 244
Fragments arranged by size:
1076 244 93
BtgI C'CryG_G
Cuts at: 0 8 1413
Size: 8 1405
BtsI GCAGTG_nn'
Cuts at: 0 632 1413
Size: 632 781
ClaI AT'CG_AT
Cuts at: 0 426 1413
Size: 426 987
DraI TTT'AAA
Cuts at: 0 1161 1413
Size: 1161 252
DrdI GACnn_nn'nnGTC
Cuts at: 0 39 1413
Size: 39 1374
EaeI y'GGCC_r
Cuts at: 0 5 1413
Size: 5 1408
EarI CTCTTCn'nnn_
Cuts at: 0 191 238 1256 1268 1283 1343 1413
Size: 191 47 1018 12 15 60 70
Fragments arranged by size:
1018 191 70 60 47 15 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1058 1413
Size: 533 525 355
Fragments arranged by size:
533 525 355
EcoO109I rG'GnC_Cy
Cuts at: 0 1033 1246 1413
Size: 1033 213 167
Fragments arranged by size:
1033 213 167
EcoRI G'AATT_C
Cuts at: 0 440 1413
Size: 440 973
EcoRV GAT'ATC
Cuts at: 0 188 1413
Size: 188 1225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 970 1413
Size: 970 443
HaeII r_GCGC'y
Cuts at: 0 28 1413
Size: 28 1385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 1413
Size: 232 32 1149
Fragments arranged by size:
1149 232 32
HindIII A'AGCT_T
Cuts at: 0 409 1413
Size: 409 1004
KasI G'GCGC_C
Cuts at: 0 24 1413
Size: 24 1389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 956 1234 1413
Size: 202 754 278 179
Fragments arranged by size:
754 278 202 179
MslI CAynn'nnrTG
Cuts at: 0 625 1413
Size: 625 788
MspA1I CmG'CkG
Cuts at: 0 30 153 538 700 1397 1413
Size: 30 123 385 162 697 16
Fragments arranged by size:
697 385 162 123 30 16
NarI GG'CG_CC
Cuts at: 0 25 1413
Size: 25 1388
NcoI C'CATG_G
Cuts at: 0 8 1413
Size: 8 1405
NdeI CA'TA_TG
Cuts at: 0 1199 1413
Size: 1199 214
NspI r_CATG'y
Cuts at: 0 714 1208 1413
Size: 714 494 205
Fragments arranged by size:
714 494 205
PciI A'CATG_T
Cuts at: 0 710 1413
Size: 710 703
PpuMI rG'GwC_Cy
Cuts at: 0 1033 1246 1413
Size: 1033 213 167
Fragments arranged by size:
1033 213 167
PstI C_TGCA'G
Cuts at: 0 639 1413
Size: 639 774
PvuII CAG'CTG
Cuts at: 0 538 700 1413
Size: 538 162 713
Fragments arranged by size:
713 538 162
SfcI C'TryA_G
Cuts at: 0 635 1226 1413
Size: 635 591 187
Fragments arranged by size:
635 591 187
SfoI GGC'GCC
Cuts at: 0 26 1413
Size: 26 1387
SmlI C'TyrA_G
Cuts at: 0 307 365 1413
Size: 307 58 1048
Fragments arranged by size:
1048 307 58
SspI AAT'ATT
Cuts at: 0 316 1413
Size: 316 1097
StuI AGG'CCT
Cuts at: 0 295 967 1413
Size: 295 672 446
Fragments arranged by size:
672 446 295
StyI C'CwwG_G
Cuts at: 0 8 1413
Size: 8 1405
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 1413
Size: 31 1382
TatI w'GTAC_w
Cuts at: 0 617 894 1413
Size: 617 277 519
Fragments arranged by size:
617 519 277
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 1413
Size: 313 32 1068
Fragments arranged by size:
1068 313 32
XhoI C'TCGA_G
Cuts at: 0 365 1413
Size: 365 1048
XmnI GAAnn'nnTTC
Cuts at: 0 1025 1413
Size: 1025 388
ZraI GAC'GTC
Cuts at: 0 528 1413
Size: 528 885
Enzymes that do cut:
AatII AcuI AflIII AlwNI ApoI AvaI BanI BanII
BbeI BbsI BcgI BciVI BglII BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BsiHKAI BsmBI
Bsp1286I BspHI BsrDI BsrGI BstYI BtgI BtsI ClaI
DraI DrdI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HindIII KasI MmeI MslI MspA1I
NarI NcoI NdeI NspI PciI PpuMI PstI PvuII
SfcI SfoI SmlI SspI StuI StyI TaqII TatI
TstI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BclI BfrBI BglI BlpI
Bme1580I BmtI BplI BsaAI BsaBI BsaWI BseYI BsgI
BsiEI BsiWI BsmI BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
CspCI DraIII EagI EciI EcoICRI EcoNI FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MscI NaeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XcmI XmaI