Restriction map for
pFN21AE3228
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BanII BbeI
BbsI BbvCI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BpuAI BsaI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII Eam1105I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MscI MunI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 293 TCCGGA T^CCGGA
AocI 1024 CCTGAGG CC^T[ACGT]AGG
AspI 1093 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 134 CCCGGG C^[CT]CG[GA]G
BbrPI 727 CACGTG CAC^GTG
BglI 755 GCCGGCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 35 AGATCT A^GATCT
BsaAI 727 CACGTG [CT]AC^GT[GA]
BsaBI 717 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 293 TCCGGA [AT]^CCGG[AT]
BseAI 293 TCCGGA T^CCGGA
BsmBI 1371 GAGACG CGTCTC(1/5)
BsoBI 134 CCCGGG C^[CT]CG[GA]G
BspEI 293 TCCGGA T^CCGGA
BspMI 878 ACCTGC ACCTGC(4/8)
BsrDI 300 GCAATG GCAATG(2/0)
BsrFI 755 GCCGGC [GA]^CCGG[CT]
BstXI 488 CCAATATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1024 CCTGAGG CC^T[ACGT]AGG
Cfr10I 755 GCCGGC [GA]^CCGG[CT]
DrdI 973 GACGCGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1024 CCTGAGG CC^T[ACGT]AGG
EcoT14I 548 CCATGG C^C[AT][AT]GG
MaeIII 1384 GTCAC ^GT[ACGT]AC
MamI 717 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 293 TCCGGA T^CCGGA
NaeI 755 GCCGGC GCC^GGC
NcoI 548 CCATGG C^CATGG
NgoMIV 755 GCCGGC G^CCGGC
NheI 1392 GCTAGC G^CTAGC
NspI 1443 GCATGT [GA]CATG^[CT]
PflFI 1093 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 727 CACGTG CAC^GTG
PmlI 727 CACGTG CAC^GTG
PshAI 1260 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 903 GAAGAGC GCTCTTC(1/4)
ScaI 1055 AGTACT AGT^ACT
SexAI 1094 ACCTGGT A^CC[AT]GGT
SfcI 473 CTACAG C^T[GA][CT]AG
SmaI 134 CCCGGG CCC^GGG
SmlI 318 CTTGAG C^T[CT][GA]AG
StuI 807 AGGCCT AGG^CCT
StyI 548 CCATGG C^C[AT][AT]GG
TfiI 1288 GAATC G^A[AT]TC
Tsp45I 1384 GTCAC ^GT[GC]AC
Tsp509I 360 AATT ^AATT
Tth111I 1093 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 134 CCCGGG C^CCGGG
XmaI 134 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 1103 AGCGCT AGC^GCT
1537 AGCGCT
BanI 42 GGTGCC G^G[CT][GA]CC
1491 GGTGCC
BclI 635 TGATCA T^GATCA
1118 TGATCA
BfaI 389 CTAG C^TAG
1393 CTAG
BsiEI 920 CGGCCG CG[GA][CT]^CG
1460 CGGCCG
BsmAI 399 GAGAC GTCTC(1/5)
1371 GAGAC
BspHI 560 TCATGA T^CATGA
1367 TCATGA
BsrBI 1363 CCGCTC CCGCTC(-3/-3)
1132 GAGCGG
BstYI 35 AGATCT [GA]^GATC[CT]
73 AGATCC
DsaI 47 CCGTGG C^C[GA][CT]GG
548 CCATGG
EaeI 920 CGGCCG [CT]^GGCC[GA]
1460 CGGCCG
EagI 920 CGGCCG C^GGCCG
1460 CGGCCG
EclXI 920 CGGCCG C^GGCCG
1460 CGGCCG
Eco47III 1103 AGCGCT AGC^GCT
1537 AGCGCT
Eco52I 920 CGGCCG C^GGCCG
1460 CGGCCG
FbaI 635 TGATCA T^GATCA
1118 TGATCA
MaeI 389 CTAG C^TAG
1393 CTAG
MflI 35 AGATCT [GA]^GATC[CT]
73 AGATCC
MseI 437 TTAA T^TAA
503 TTAA
PflMI 1089 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1101 CCAGCGCTTGG
RcaI 560 TCATGA T^CATGA
1367 TCATGA
Tru9I 437 TTAA T^TAA
503 TTAA
Van91I 1089 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1101 CCAGCGCTTGG
XhoII 35 AGATCT [GA]^GATC[CT]
73 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
TspRI TthHB8I