(Circular) (Six-base) MAPSORT of: pFN21AE3228.seq Check: 4000 from: 1 to: 6265 pFN21AE3228 6265 bp With 182 enzymes: SgfI * June 19, 2019 18:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4075 278 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3608 1937 Size: 1671 4594 Acc65I G'GTAC_C Cuts at: 3586 3586 Size: 6265 AclI AA'CG_TT Cuts at: 1563 4392 4765 1563 Size: 2829 373 3063 Fragments arranged by size: 3063 2829 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2897 2927 4328 5712 5980 1632 Size: 1265 30 1401 1384 268 1917 Fragments arranged by size: 1917 1401 1384 1265 268 30 AfeI AGC'GCT Cuts at: 3110 3544 3110 Size: 434 5831 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6068 AflIII A'CryG_T Cuts at: 1203 3562 5164 6141 1203 Size: 2359 1602 977 1327 Fragments arranged by size: 2359 1602 1327 977 AgeI A'CCGG_T Cuts at: 5096 5096 Size: 6265 AhdI GACnn_n'nnGTC Cuts at: 4994 4994 Size: 6265 AleI CACnn'nnGTG Cuts at: 1166 2204 1166 Size: 1038 5227 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3299 3331 3299 Size: 32 6233 AlwNI CAG_nnn'CTG Cuts at: 2082 2875 3100 4008 5580 2082 Size: 793 225 908 1572 2767 Fragments arranged by size: 2767 1572 908 793 225 ApaLI G'TGCA_C Cuts at: 4322 5478 4322 Size: 1156 5109 ApoI r'AATT_y Cuts at: 1493 3574 3800 3952 6061 1493 Size: 2081 226 152 2109 1697 Fragments arranged by size: 2109 2081 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4819 160 Size: 872 3787 1606 Fragments arranged by size: 3787 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 AvaI C'yCGr_G Cuts at: 1870 1943 2139 3590 1870 Size: 73 196 1451 4545 Fragments arranged by size: 4545 1451 196 73 BamHI G'GATC_C Cuts at: 3595 3962 3595 Size: 367 5898 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2047 3496 3586 5041 618 Size: 325 217 627 260 1449 90 1455 1842 Fragments arranged by size: 1842 1455 1449 627 325 260 217 90 BanII G_rGCy'C Cuts at: 1391 3584 1391 Size: 2193 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5294 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4456 4490 1903 Size: 34 50 34 2435 34 3678 Fragments arranged by size: 3678 2435 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4157 5367 4157 Size: 1210 5055 BclI T'GATC_A Cuts at: 1548 2640 3123 1548 Size: 1092 483 4690 Fragments arranged by size: 4690 1092 483 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2766 4876 136 Size: 107 122 71 631 605 1094 2110 1525 Fragments arranged by size: 2110 1525 1094 631 605 122 107 71 BglII A'GATC_T Cuts at: 2040 6260 2040 Size: 4220 2045 BlpI GC'TnA_GC Cuts at: 3682 3682 Size: 6265 Bme1580I G_kGCm'C Cuts at: 1790 2052 3501 4326 5482 1790 Size: 262 1449 825 1156 2573 Fragments arranged by size: 2573 1449 1156 825 262 BmgBI CAC'GTC Cuts at: 1318 2656 1318 Size: 1338 4927 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3996 4954 442 Size: 416 53 83 399 24 2579 958 1753 Fragments arranged by size: 2579 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3401 1056 Size: 2345 3920 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2500 2771 3155 3538 4925 1239 Size: 1261 271 384 383 1387 2579 Fragments arranged by size: 2579 1387 1261 384 383 271 Bpu10I CC'TnA_GC Cuts at: 2314 2314 Size: 6265 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2344 4390 5255 5553 2344 Size: 2046 865 298 3056 Fragments arranged by size: 3056 2046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4928 882 Size: 617 3429 2219 Fragments arranged by size: 3429 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2734 4056 493 Size: 711 1530 1322 2702 Fragments arranged by size: 2702 1530 1322 711 BsaBI GATnn'nnATC Cuts at: 2726 6259 2726 Size: 3533 2732 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2193 2985 3266 4072 Size: 53 83 186 936 660 792 281 806 Cuts at: 4072 4454 5089 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 806 792 660 635 382 281 186 83 53 BsaWI w'CCGG_w Cuts at: 2298 4698 5096 5370 5517 5928 2298 Size: 2400 398 274 147 411 2635 Fragments arranged by size: 2635 2400 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6040 Fragments arranged by size: 6040 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2416 2879 3008 3023 3047 3233 3458 3541 Size: 1230 463 129 15 24 186 225 83 Cuts at: 3541 1186 Size: 3910 Fragments arranged by size: 3910 1230 463 225 186 129 83 24 15 BseYI C'CCAG_C Cuts at: 3476 5468 5964 3476 Size: 1992 496 3777 Fragments arranged by size: 3777 1992 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 2705 2756 3234 3307 1163 Size: 573 318 651 51 478 73 4121 Fragments arranged by size: 4121 651 573 478 318 73 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2928 3468 3715 4476 5504 1540 Size: 275 186 927 540 247 761 1028 2301 Fragments arranged by size: 2301 1028 927 761 540 275 247 186 BsiHKAI G_wGCw'C Cuts at: 2781 2848 2915 4326 4411 5482 2781 Size: 67 67 1411 85 1071 3564 Fragments arranged by size: 3564 1411 1071 85 67 67 BsmI GAATG_Cn' Cuts at: 3776 3869 3776 Size: 93 6172 BsmBI CGTCTCn'nnnn_ Cuts at: 3370 3370 Size: 6265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2781 2848 2915 3501 3584 4326 Size: 399 262 729 67 67 586 83 742 Cuts at: 4326 4411 5482 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 729 586 399 262 85 83 67 67 BspEI T'CCGG_A Cuts at: 2298 5928 2298 Size: 3630 2635 BspHI T'CATG_A Cuts at: 2565 3372 4154 5884 2565 Size: 807 782 1730 2946 Fragments arranged by size: 2946 1730 807 782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2892 3620 844 Size: 2048 728 3489 Fragments arranged by size: 3489 2048 728 BsrBI CCG'CTC Cuts at: 1394 1823 3139 3370 4152 1394 Size: 429 1316 231 782 3507 Fragments arranged by size: 3507 1316 782 429 231 BsrDI GCAATG_nn' Cuts at: 65 1248 2312 4760 4934 65 Size: 1183 1064 2448 174 1396 Fragments arranged by size: 2448 1396 1183 1064 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2760 4909 5096 1700 Size: 110 950 2149 187 2869 Fragments arranged by size: 2869 2149 950 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6265 BssSI C'ACGA_G Cuts at: 4325 5337 4325 Size: 1012 5253 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6265 BstBI TT'CG_AA Cuts at: 5069 5069 Size: 6265 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6265 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2500 2011 Size: 489 5776 BstYI r'GATC_y Cuts at: 1972 2040 2078 3595 3962 4347 4364 5816 5902 Size: 68 38 1517 367 385 17 1452 86 Cuts at: 5902 5914 6260 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1517 1452 385 367 346 86 68 38 17 12 Bsu36I CC'TnA_GG Cuts at: 3030 3030 Size: 6265 BtgI C'CryG_G Cuts at: 513 1065 2052 2553 513 Size: 552 987 501 4225 Fragments arranged by size: 4225 987 552 501 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5526 Fragments arranged by size: 5526 683 56 BtsI GCAGTG_nn' Cuts at: 2960 3781 4575 4595 2960 Size: 821 794 20 4630 Fragments arranged by size: 4630 821 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6230 DraI TTT'AAA Cuts at: 3569 3569 Size: 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2984 5272 809 Size: 507 1668 2288 1802 Fragments arranged by size: 2288 1802 1668 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2925 3465 3712 Size: 54 1425 50 275 18 1095 540 247 Cuts at: 3712 4601 6230 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1095 889 540 275 247 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2925 3465 3712 1537 Size: 275 1113 540 247 4090 Fragments arranged by size: 4090 1113 540 275 247 EarI CTCTTCn'nnn_ Cuts at: 1353 2564 2903 3249 4195 1353 Size: 1211 339 346 946 3423 Fragments arranged by size: 3423 1211 946 346 339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2258 4852 5226 5372 387 Size: 1064 138 669 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 669 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2500 2771 2897 2927 3155 3538 4328 Size: 393 868 271 126 30 228 383 790 Cuts at: 4328 4925 5712 5980 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 868 790 787 597 393 383 271 268 228 126 30 EcoO109I rG'GnC_Cy Cuts at: 2064 2881 3097 3359 2064 Size: 817 216 262 4970 Fragments arranged by size: 4970 817 262 216 EcoRI G'AATT_C Cuts at: 3574 3952 3574 Size: 378 5887 FspI TGC'GCA Cuts at: 4771 4771 Size: 6265 HaeII r_GCGC'y Cuts at: 1678 2936 2966 3112 3546 5412 1678 Size: 1258 30 146 434 1866 2531 Fragments arranged by size: 2531 1866 1258 434 146 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2397 2429 2525 2557 4906 Size: 32 135 32 825 32 96 32 2349 Cuts at: 4906 4938 4980 5012 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2349 825 135 96 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3609 3855 669 Size: 988 281 1671 246 3079 Fragments arranged by size: 3079 1671 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6265 HpaI GTT'AAC Cuts at: 3855 3855 Size: 6265 KpnI G_GTAC'C Cuts at: 3590 3590 Size: 6265 MfeI C'AATT_G Cuts at: 3864 3864 Size: 6265 MluI A'CGCG_T Cuts at: 6141 6141 Size: 6265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1992 5379 5563 1305 Size: 687 3387 184 2007 Fragments arranged by size: 3387 2007 687 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4443 Fragments arranged by size: 4443 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2204 2732 4223 4582 4741 518 Size: 648 954 84 528 1491 359 159 2042 Fragments arranged by size: 2042 1491 954 648 528 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2905 3480 3508 3681 4358 5506 5751 1606 Size: 1299 575 28 173 677 1148 245 2120 Fragments arranged by size: 2120 1299 1148 677 575 245 173 28 NaeI GCC'GGC Cuts at: 2762 2762 Size: 6265 NcoI C'CATG_G Cuts at: 513 1065 2553 513 Size: 552 1488 4225 Fragments arranged by size: 4225 1488 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6265 NgoMIV G'CCGG_C Cuts at: 2760 2760 Size: 6265 NheI G'CTAG_C Cuts at: 1052 3397 1052 Size: 2345 3920 NotI GC'GGCC_GC Cuts at: 3712 3712 Size: 6265 NspI r_CATG'y Cuts at: 1132 1229 3452 3623 5168 1132 Size: 97 2223 171 1545 2229 Fragments arranged by size: 2229 2223 1545 171 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6265 PciI A'CATG_T Cuts at: 5164 5164 Size: 6265 PflMI CCAn_nnn'nTGG Cuts at: 1108 3100 3112 1108 Size: 1992 12 4261 Fragments arranged by size: 4261 1992 12 PfoI T'CCnGG_A Cuts at: 3201 3533 3554 3201 Size: 332 21 5912 Fragments arranged by size: 5912 332 21 PmeI GTTT'AAAC Cuts at: 3569 3569 Size: 6265 PmlI CAC'GTG Cuts at: 2734 2734 Size: 6265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4334 4366 5847 5879 4334 Size: 32 1481 32 4720 Fragments arranged by size: 4720 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2881 3097 3359 2881 Size: 216 262 5787 Fragments arranged by size: 5787 262 216 PshAI GACnn'nnGTC Cuts at: 1613 3269 1613 Size: 1656 4609 PsiI TTA'TAA Cuts at: 3835 3835 Size: 6265 PstI C_TGCA'G Cuts at: 830 3617 830 Size: 2787 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6265 PvuII CAG'CTG Cuts at: 2905 3480 3508 2905 Size: 575 28 5662 Fragments arranged by size: 5662 575 28 SalI G'TCGA_C Cuts at: 1936 3607 1936 Size: 1671 4594 SapI GCTCTTCn'nnn_ Cuts at: 2903 2903 Size: 6265 SbfI CC_TGCA'GG Cuts at: 3617 3617 Size: 6265 ScaI AGT'ACT Cuts at: 3062 4513 6225 3062 Size: 1451 1712 3102 Fragments arranged by size: 3102 1712 1451 SexAI A'CCwGG_T Cuts at: 3099 3099 Size: 6265 SfcI C'TryA_G Cuts at: 826 1045 2478 3613 4748 5429 5620 826 Size: 219 1433 1135 1135 681 191 1471 Fragments arranged by size: 1471 1433 1135 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6265 SmaI CCC'GGG Cuts at: 1872 2141 3592 1872 Size: 269 1451 4545 Fragments arranged by size: 4545 1451 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 2323 4369 5270 5532 820 Size: 197 926 380 2046 901 262 1553 Fragments arranged by size: 2046 1553 926 901 380 262 197 SnaBI TAC'GTA Cuts at: 493 4056 493 Size: 3563 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6265 SphI G_CATG'C Cuts at: 1132 3623 1132 Size: 2491 3774 SspI AAT'ATT Cuts at: 5 52 4189 5 Size: 47 4137 2081 Fragments arranged by size: 4137 2081 47 StuI AGG'CCT Cuts at: 1517 2814 1517 Size: 1297 4968 StyI C'CwwG_G Cuts at: 513 1065 2553 3704 513 Size: 552 1488 1151 3074 Fragments arranged by size: 3074 1488 1151 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2035 4292 4309 4462 4647 5092 325 Size: 1255 455 2257 17 153 185 445 1498 Fragments arranged by size: 2257 1498 1255 455 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 2712 3060 Size: 274 80 33 51 1443 270 465 348 Cuts at: 3060 3151 4511 6223 96 Size: 91 1360 1712 138 Fragments arranged by size: 1712 1443 1360 465 348 274 270 138 91 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2293 2325 6225 6257 2293 Size: 32 3900 32 2301 Fragments arranged by size: 3900 2301 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3101 1316 Size: 1785 4480 XbaI T'CTAG_A Cuts at: 3601 3601 Size: 6265 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6265 XmaI C'CCGG_G Cuts at: 1870 2139 3590 1870 Size: 269 1451 4545 Fragments arranged by size: 4545 1451 269 XmnI GAAnn'nnTTC Cuts at: 4394 4394 Size: 6265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4073 276 Size: 53 83 186 936 2539 2468 Fragments arranged by size: 2539 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI