Restriction map for
pFN21AE3228
(Linear) (Six-base) MAPSORT of: KIEE3228.seq Check: 5671 from: 1 to: 1560
KIEE3228.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 922 1560
Size: 892 30 638
Fragments arranged by size:
892 638 30
AfeI AGC'GCT
Cuts at: 0 1105 1539 1560
Size: 1105 434 21
Fragments arranged by size:
1105 434 21
AleI CACnn'nnGTG
Cuts at: 0 199 1560
Size: 199 1361
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1294 1326 1560
Size: 1294 32 234
Fragments arranged by size:
1294 234 32
AlwNI CAG_nnn'CTG
Cuts at: 0 77 870 1095 1560
Size: 77 793 225 465
Fragments arranged by size:
793 465 225 77
AvaI C'yCGr_G
Cuts at: 0 134 1560
Size: 134 1426
BanI G'GyrC_C
Cuts at: 0 42 1491 1560
Size: 42 1449 69
Fragments arranged by size:
1449 69 42
BclI T'GATC_A
Cuts at: 0 635 1118 1560
Size: 635 483 442
Fragments arranged by size:
635 483 442
BglI GCCn_nnn'nGGC
Cuts at: 0 761 1560
Size: 761 799
BglII A'GATC_T
Cuts at: 0 35 1560
Size: 35 1525
Bme1580I G_kGCm'C
Cuts at: 0 47 1496 1560
Size: 47 1449 64
Fragments arranged by size:
1449 64 47
BmgBI CAC'GTC
Cuts at: 0 651 1560
Size: 651 909
BmtI G_CTAG'C
Cuts at: 0 1396 1560
Size: 1396 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 766 1150 1533 1560
Size: 495 271 384 383 27
Fragments arranged by size:
495 384 383 271 27
Bpu10I CC'TnA_GC
Cuts at: 0 309 1560
Size: 309 1251
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 1560
Size: 339 1221
BsaAI yAC'GTr
Cuts at: 0 729 1560
Size: 729 831
BsaBI GATnn'nnATC
Cuts at: 0 721 1560
Size: 721 839
BsaHI Gr'CG_yC
Cuts at: 0 188 980 1261 1560
Size: 188 792 281 299
Fragments arranged by size:
792 299 281 188
BsaWI w'CCGG_w
Cuts at: 0 293 1560
Size: 293 1267
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 411 874 1003 1018 1042 1228 1453 1536
Size: 411 463 129 15 24 186 225 83
Cuts at: 1536 1560
Size: 24
Fragments arranged by size:
463 411 225 186 129 83 24 24
15
BseYI C'CCAG_C
Cuts at: 0 1471 1560
Size: 1471 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 700 751 1229 1302 1560
Size: 49 651 51 478 73 258
Fragments arranged by size:
651 478 258 73 51 49
BsiEI CG_ry'CG
Cuts at: 0 923 1463 1560
Size: 923 540 97
Fragments arranged by size:
923 540 97
BsiHKAI G_wGCw'C
Cuts at: 0 776 843 910 1560
Size: 776 67 67 650
Fragments arranged by size:
776 650 67 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1365 1560
Size: 1365 195
Bsp1286I G_dGCh'C
Cuts at: 0 47 776 843 910 1496 1560
Size: 47 729 67 67 586 64
Fragments arranged by size:
729 586 67 67 64 47
BspEI T'CCGG_A
Cuts at: 0 293 1560
Size: 293 1267
BspHI T'CATG_A
Cuts at: 0 560 1367 1560
Size: 560 807 193
Fragments arranged by size:
807 560 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 887 1560
Size: 887 673
BsrBI CCG'CTC
Cuts at: 0 1134 1365 1560
Size: 1134 231 195
Fragments arranged by size:
1134 231 195
BsrDI GCAATG_nn'
Cuts at: 0 307 1560
Size: 307 1253
BsrFI r'CCGG_y
Cuts at: 0 755 1560
Size: 755 805
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 1560
Size: 495 1065
BstYI r'GATC_y
Cuts at: 0 35 73 1560
Size: 35 38 1487
Fragments arranged by size:
1487 38 35
Bsu36I CC'TnA_GG
Cuts at: 0 1025 1560
Size: 1025 535
BtgI C'CryG_G
Cuts at: 0 47 548 1560
Size: 47 501 1012
Fragments arranged by size:
1012 501 47
BtsI GCAGTG_nn'
Cuts at: 0 955 1560
Size: 955 605
DrdI GACnn_nn'nnGTC
Cuts at: 0 979 1560
Size: 979 581
EaeI y'GGCC_r
Cuts at: 0 920 1460 1560
Size: 920 540 100
Fragments arranged by size:
920 540 100
EagI C'GGCC_G
Cuts at: 0 920 1460 1560
Size: 920 540 100
Fragments arranged by size:
920 540 100
EarI CTCTTCn'nnn_
Cuts at: 0 559 898 1244 1560
Size: 559 339 346 316
Fragments arranged by size:
559 346 339 316
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 253 1560
Size: 253 1307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 766 892 922 1150 1533 1560
Size: 495 271 126 30 228 383 27
Fragments arranged by size:
495 383 271 228 126 30 27
EcoO109I rG'GnC_Cy
Cuts at: 0 59 876 1092 1354 1560
Size: 59 817 216 262 206
Fragments arranged by size:
817 262 216 206 59
HaeII r_GCGC'y
Cuts at: 0 931 961 1107 1541 1560
Size: 931 30 146 434 19
Fragments arranged by size:
931 434 146 30 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 392 424 520 552 1560
Size: 392 32 96 32 1008
Fragments arranged by size:
1008 392 96 32 32
MslI CAynn'nnrTG
Cuts at: 0 115 199 727 1560
Size: 115 84 528 833
Fragments arranged by size:
833 528 115 84
MspA1I CmG'CkG
Cuts at: 0 900 1475 1503 1560
Size: 900 575 28 57
Fragments arranged by size:
900 575 57 28
NaeI GCC'GGC
Cuts at: 0 757 1560
Size: 757 803
NcoI C'CATG_G
Cuts at: 0 548 1560
Size: 548 1012
NgoMIV G'CCGG_C
Cuts at: 0 755 1560
Size: 755 805
NheI G'CTAG_C
Cuts at: 0 1392 1560
Size: 1392 168
NspI r_CATG'y
Cuts at: 0 1447 1560
Size: 1447 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 1095 1107 1560
Size: 1095 12 453
Fragments arranged by size:
1095 453 12
PfoI T'CCnGG_A
Cuts at: 0 1196 1528 1549 1560
Size: 1196 332 21 11
Fragments arranged by size:
1196 332 21 11
PmlI CAC'GTG
Cuts at: 0 729 1560
Size: 729 831
PpuMI rG'GwC_Cy
Cuts at: 0 876 1092 1354 1560
Size: 876 216 262 206
Fragments arranged by size:
876 262 216 206
PshAI GACnn'nnGTC
Cuts at: 0 1264 1560
Size: 1264 296
PvuII CAG'CTG
Cuts at: 0 900 1475 1503 1560
Size: 900 575 28 57
Fragments arranged by size:
900 575 57 28
SapI GCTCTTCn'nnn_
Cuts at: 0 898 1560
Size: 898 662
ScaI AGT'ACT
Cuts at: 0 1057 1560
Size: 1057 503
SexAI A'CCwGG_T
Cuts at: 0 1094 1560
Size: 1094 466
SfcI C'TryA_G
Cuts at: 0 473 1560
Size: 473 1087
SmaI CCC'GGG
Cuts at: 0 136 1560
Size: 136 1424
SmlI C'TyrA_G
Cuts at: 0 318 1560
Size: 318 1242
StuI AGG'CCT
Cuts at: 0 809 1560
Size: 809 751
StyI C'CwwG_G
Cuts at: 0 548 1560
Size: 548 1012
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 30 1560
Size: 30 1530
TatI w'GTAC_w
Cuts at: 0 242 707 1055 1146 1560
Size: 242 465 348 91 414
Fragments arranged by size:
465 414 348 242 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 288 320 1560
Size: 288 32 1240
Fragments arranged by size:
1240 288 32
Tth111I GACn'n_nGTC
Cuts at: 0 1096 1560
Size: 1096 464
XmaI C'CCGG_G
Cuts at: 0 134 1560
Size: 134 1426
Enzymes that do cut:
AcuI AfeI AleI AloI AlwNI AvaI BanI BclI
BglI BglII Bme1580I BmgBI BmtI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PflMI PfoI
PmlI PpuMI PshAI PvuII SapI ScaI SexAI SfcI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BfrBI BlpI BmrI BplI BsaI BsaXI BsiWI
BsmI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NdeI
NotI NruI NsiI PacI PasI PciI PmeI PpiI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI
XhoI XmnI ZraI