################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstF5I BstPI BstUI BstYI Bsu36I Cac8I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeIII HgaI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI MwoI NaeI NcoI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 321 GTATAC GT^[AC][GT]AC AcyI 61 GGCGCC G[GA]^CG[CT]C AfaI 120 GTAC GT^AC AflIII 88 ACGTGT A^C[GA][CT]GT AlwI 536 GGATC GGATC(4/5) AlwNI 361 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 254 GAGCAC G[AT]GC[AT]^C AspI 490 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 27 GGACC G^G[AT]CC BanI 61 GGCGCC G^G[CT][GA]CC BbeI 61 GGCGCC GGCGC^C BbvI 520 GCTGC GCAGC(8/12) BcnI 68 CCGGG CC^[GC]GG BfaI 638 CTAG C^TAG BmyI 254 GAGCAC G[AGT]GC[ACT]^C BsaAI 87 TACGTG [CT]AC^GT[GA] BsaHI 61 GGCGCC G[GA]^CG[CT]C BsgI 653 GTGCAG GTGCAG(16/14) BsiHKAI 254 GAGCAC G[AT]GC[AT]^C BsmFI 56 GGGAC GGGAC(10/14) Bsp1286I 254 GAGCAC G[AGT]GC[ACT]^C BsrDI 164 CATTGC GCAATG(2/0) BsrFI 29 ACCGGC [GA]^CCGG[CT] Bst1107I 321 GTATAC GTA^TAC BstXI 444 CCAGGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 321 GTATAC GTA^TAC CfoI 62 GCGC GCG^C Cfr10I 29 ACCGGC [GA]^CCGG[CT] Cfr13I 27 GGACC G^G[ACGT]CC DpnII 537 GATC ^GATC Fnu4HI 520 GCTGC GC^[ACGT]GC HaeII 61 GGCGCC [GA]GCGC^[CT] HhaI 62 GCGC GCG^C Hin1I 61 GGCGCC G[GA]^CG[CT]C HinP1I 62 GCGC G^CGC HincII 628 GTTAAC GT[CT]^[GA]AC HindII 628 GTTAAC GT[CT]^[GA]AC HindIII 297 AAGCTT A^AGCTT HpaI 628 GTTAAC GTT^AAC ItaI 520 GCTGC GC^[ACGT]GC KasI 61 GGCGCC G^GCGCC MaeI 638 CTAG C^TAG MaeII 88 ACGT A^CGT MboI 537 GATC ^GATC NarI 61 GGCGCC GG^CGCC NciI 68 CCGGG CC^[GC]GG NdeI 403 CATATG CA^TATG NdeII 537 GATC ^GATC PflFI 490 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 120 GTAC GT^AC Sau3AI 537 GATC ^GATC Sau96I 27 GGACC G^G[ACGT]CC SfcI 131 CTATAG C^T[GA][CT]AG SfoI 61 GGCGCC GGC^GCC SmlI 98 CTCAAG C^T[CT][GA]AG TseI 520 GCTGC G^C[AT]GC Tsp45I 567 GTCAC ^GT[GC]AC TspRI 365 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 490 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 194 AAATTT [GA]^AATT[CT] 306 GAATTT AluI 298 AGCT AG^CT 643 AGCT ApoI 194 AAATTT [GA]^AATT[CT] 306 GAATTT BbsI 72 GAAGAC GAAGAC(2/6) 496 GTCTTC BpuAI 72 GAAGAC GAAGAC(2/6) 496 GTCTTC BsaJI 374 CCTTGG C^C[ACGT][ACGT]GG 531 CCTTGG BsmAI 488 GAGAC GTCTC(1/5) 472 GTCTC BsrI 138 ACTGG ACTGG(1/-1) 366 ACTGG EcoT14I 374 CCTTGG C^C[AT][AT]GG 531 CCTTGG HapII 30 CCGG C^CGG 68 CCGG HpaII 30 CCGG C^CGG 68 CCGG HphI 330 GGTGA GGTGA(8/7) 579 GGTGA MaeIII 184 GTTAC ^GT[ACGT]AC 567 GTCAC MspI 30 CCGG C^CGG 68 CCGG NlaIV 61 GGCGCC GG[ACGT]^[ACGT]CC 609 GGAACC StyI 374 CCTTGG C^C[AT][AT]GG 531 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### BseRI BssKI BstNI DdeI EcoRII HinfI MboII MnlI MseI MvaI PleI ScrFI Tru9I Tsp509I