Restriction map for
pFN21AE3059
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BanI BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MslI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 221 CGCG CG^CG
AcsI 28 AAATTC [GA]^AATT[CT]
AlwNI 50 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 451 AGCGCT AGC^GCT
ApoI 28 AAATTC [GA]^AATT[CT]
Asp700I 90 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 140 CCCGGG C^[CT]CG[GA]G
BamHI 136 GGATCC G^GATCC
BbsI 162 GAAGAC GAAGAC(2/6)
BbvCI 622 CCTCAGC CCTCAGC(-5/-2)
BfaI 532 CTAG C^TAG
BpuAI 162 GAAGAC GAAGAC(2/6)
BseRI 475 GAGGAG GAGGAG(10/8)
BsgI 47 GTGCAG GTGCAG(16/14)
BsmI 557 GCATTC GAATGC(1/-1)
BsoBI 140 CCCGGG C^[CT]CG[GA]G
BstUI 221 CGCG CG^CG
BstXI 335 CCAACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 136 GGATCC [GA]^GATC[CT]
CfoI 452 GCGC GCG^C
DpnII 137 GATC ^GATC
DraII 368 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 614 CCGTGG C^C[GA][CT]GG
EarI 22 CTCTTC CTCTTC(1/4)
Eco47III 451 AGCGCT AGC^GCT
EcoO109I 368 AGGACCT [GA]G^G[ACGT]CC[CT]
HaeII 451 AGCGCT [GA]GCGC^[CT]
HhaI 452 GCGC GCG^C
HinP1I 452 GCGC G^CGC
HincII 148 GTCAAC GT[CT]^[GA]AC
HindII 148 GTCAAC GT[CT]^[GA]AC
Ksp632I 22 CTCTTC CTCTTC(1/4)
MaeI 532 CTAG C^TAG
MboI 137 GATC ^GATC
MflI 136 GGATCC [GA]^GATC[CT]
MvnI 221 CGCG CG^CG
NdeII 137 GATC ^GATC
NlaIII 65 CATG CATG^
PflMI 609 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 368 AGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 137 GATC ^GATC
SfcI 175 CTACAG C^T[GA][CT]AG
SmaI 140 CCCGGG CCC^GGG
SmlI 487 CTCAAG C^T[CT][GA]AG
StuI 404 AGGCCT AGG^CCT
Tsp509I 29 AATT ^AATT
Van91I 609 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 136 GGATCC [GA]^GATC[CT]
XmaCI 140 CCCGGG C^CCGGG
XmaI 140 CCCGGG C^CCGGG
XmnI 90 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 261 GTAC GT^AC
564 GTAC
AlwI 137 GATCC GGATC(4/5)
136 GGATC
AvaII 369 GGACC G^G[AT]CC
375 GGACC
BalI 195 TGGCCA TGG^CCA
326 TGGCCA
BanII 248 GAGCCC G[GA]GC[CT]^C
549 GGGCTC
BsaI 7 GAGACC GGTCTC(1/5)
445 GAGACC
BsmFI 185 GGGAC GGGAC(10/14)
576 GTCCC
BsrBI 202 GAGCGG CCGCTC(-3/-3)
382 GAGCGG
EaeI 195 TGGCCA [CT]^GGCC[GA]
326 TGGCCA
HgaI 219 GACGC GACGC(5/10)
574 GCGTC
HinfI 106 GACTC G^A[ACGT]TC
520 GACTC
HphI 36 GGTGA GGTGA(8/7)
269 TCACC
MaeII 152 ACGT A^CGT
470 ACGT
MaeIII 217 GTGAC ^GT[ACGT]AC
391 GTCAC
MboII 162 GAAGA GAAGA(8/7)
23 TCTTC
MluNI 195 TGGCCA TGG^CCA
326 TGGCCA
MscI 195 TGGCCA TGG^CCA
326 TGGCCA
MwoI 241 GCGGCTGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
588 GCTTCGGCAGC
PleI 106 GACTC GAGTC(4/5)
520 GACTC
RsaI 261 GTAC GT^AC
564 GTAC
SfaNI 313 GATGC GCATC(5/9)
571 GATGC
Tsp45I 217 GTGAC ^GT[GC]AC
391 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MnlI MspI MvaI NciI
NlaIV Sau96I ScrFI TseI TspRI