Restriction map for
pFN21AE3059
(Circular) (Six-base) MAPSORT of: pFN21AE3059.seq Check: 7839 from: 1 to: 5344
pFN21AE3059 5344 bp
With 182 enzymes: SgfI *
March 28, 2012 12:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3154 278
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2687 1937
Size: 750 4594
Acc65I G'GTAC_C
Cuts at: 2665 2665
Size: 5344
AclI AA'CG_TT
Cuts at: 1563 3471 3844 1563
Size: 1908 373 3063
Fragments arranged by size:
3063 1908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3407 4791 5059 1632
Size: 1775 1384 268 1917
Fragments arranged by size:
1917 1775 1384 268
AfeI AGC'GCT
Cuts at: 2458 2458
Size: 5344
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5147
AflIII A'CryG_T
Cuts at: 1203 4243 5220 1203
Size: 3040 977 1327
Fragments arranged by size:
3040 1327 977
AgeI A'CCGG_T
Cuts at: 4175 4175
Size: 5344
AhdI GACnn_n'nnGTC
Cuts at: 4073 4073
Size: 5344
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5344
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2471 2503 2471
Size: 32 5312
AlwNI CAG_nnn'CTG
Cuts at: 2060 3087 4659 2060
Size: 1027 1572 2745
Fragments arranged by size:
2745 1572 1027
ApaLI G'TGCA_C
Cuts at: 3401 4557 3401
Size: 1156 4188
ApoI r'AATT_y
Cuts at: 1493 2033 2653 2879 3031 5140 1493
Size: 540 620 226 152 2109 1697
Fragments arranged by size:
2109 1697 620 540 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3898 160
Size: 872 2866 1606
Fragments arranged by size:
2866 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2669 1870
Size: 73 202 524 4545
Fragments arranged by size:
4545 524 202 73
BamHI G'GATC_C
Cuts at: 2141 2674 3041 2141
Size: 533 367 4444
Fragments arranged by size:
4444 533 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2665 4120 618
Size: 325 217 627 878 1455 1842
Fragments arranged by size:
1842 1455 878 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2257 2558 2663 1391
Size: 866 301 105 4072
Fragments arranged by size:
4072 866 301 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2174 928
Size: 971 275 4098
Fragments arranged by size:
4098 971 275
BbvCI CC'TCA_GC
Cuts at: 2628 2628
Size: 5344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3535 3569 1903
Size: 34 1598 34 3678
Fragments arranged by size:
3678 1598 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3236 4446 3236
Size: 1210 4134
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3955 136
Size: 107 122 71 631 605 2283 1525
Fragments arranged by size:
2283 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5339 5339
Size: 5344
BlpI GC'TnA_GC
Cuts at: 2761 2761
Size: 5344
Bme1580I G_kGCm'C
Cuts at: 1790 2503 2614 3405 4561 1790
Size: 713 111 791 1156 2573
Fragments arranged by size:
2573 1156 791 713 111
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3075 4033 442
Size: 416 53 83 399 24 1658 958 1753
Fragments arranged by size:
1753 1658 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2271 2426 2468 4004 1239
Size: 1032 155 42 1536 2579
Fragments arranged by size:
2579 1536 1032 155 42
Bpu10I CC'TnA_GC
Cuts at: 2385 2628 2385
Size: 243 5101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 3469 4334 4632 2477
Size: 992 865 298 3189
Fragments arranged by size:
3189 992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2006 2444 4007 882
Size: 617 507 438 1563 2219
Fragments arranged by size:
2219 1563 617 507 438
BsaAI yAC'GTr
Cuts at: 493 1204 3135 493
Size: 711 1931 2702
Fragments arranged by size:
2702 1931 711
BsaBI GATnn'nnATC
Cuts at: 5338 5338
Size: 5344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3151 3533 4168 275
Size: 53 83 186 936 1618 382 635 1451
Fragments arranged by size:
1618 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3777 4175 4449 4596 5007 3777
Size: 398 274 147 411 4114
Fragments arranged by size:
4114 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2471 2501 1178
Size: 30 165 30 1068 30 4021
Fragments arranged by size:
4021 1068 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 1186
Size: 1309 4035
BseYI C'CCAG_C
Cuts at: 4547 5043 4547
Size: 496 4848
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2073 1163
Size: 573 337 4434
Fragments arranged by size:
4434 573 337
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2794 3555 4583 1540
Size: 275 186 793 761 1028 2301
Fragments arranged by size:
2301 1028 793 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3405 3490 4561 3405
Size: 85 1071 4188
Fragments arranged by size:
4188 1071 85
BsmI GAATG_Cn'
Cuts at: 2562 2855 2948 2562
Size: 293 93 4958
Fragments arranged by size:
4958 293 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2257 2503 2558 2614 2663 3405 3490
Size: 399 467 246 55 56 49 742 85
Cuts at: 3490 4561 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 467 399 246 85 56
55 49
BspEI T'CCGG_A
Cuts at: 5007 5007
Size: 5344
BspHI T'CATG_A
Cuts at: 3233 4963 3233
Size: 1730 3614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 844
Size: 1855 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2209 2389 3231 1394
Size: 429 386 180 842 3507
Fragments arranged by size:
3507 842 429 386 180
BsrDI GCAATG_nn'
Cuts at: 65 1248 3839 4013 65
Size: 1183 2591 174 1396
Fragments arranged by size:
2591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3988 4175 1700
Size: 110 2178 187 2869
Fragments arranged by size:
2869 2178 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5344
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5344
BssSI C'ACGA_G
Cuts at: 3404 4416 3404
Size: 1012 4332
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5344
BstBI TT'CG_AA
Cuts at: 4148 4148
Size: 5344
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5344
BstXI CCAn_nnnn'nTGG
Cuts at: 2347 2347
Size: 5344
BstYI r'GATC_y
Cuts at: 1972 2141 2674 3041 3426 3443 4895 4981 4993
Size: 169 533 367 385 17 1452 86 12
Cuts at: 4993 5339 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 533 385 367 346 169 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2619 513
Size: 552 939 615 3238
Fragments arranged by size:
3238 939 615 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4605
Fragments arranged by size:
4605 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 2860 3654 3674 2056
Size: 804 794 20 3726
Fragments arranged by size:
3726 804 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2112 2143 2147 2178 529
Size: 35 1548 31 4 31 3695
Fragments arranged by size:
3695 1548 35 31 31 4
DraI TTT'AAA
Cuts at: 2648 2648
Size: 5344
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4351 809
Size: 507 3035 1802
Fragments arranged by size:
3035 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2200 2331 2791
Size: 54 1425 50 275 18 370 131 460
Cuts at: 2791 3680 5309 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 460 370 275 131 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2791 1537
Size: 275 979 4090
Fragments arranged by size:
4090 979 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2033 3274 1353
Size: 680 1241 3423
Fragments arranged by size:
3423 1241 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2576 3931 4305 4451 387
Size: 1064 138 987 1355 374 146 1280
Fragments arranged by size:
1355 1280 1064 987 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2426 2468 3407 4004 4791 5059
Size: 393 639 155 42 939 597 787 268
Cuts at: 5059 1239
Size: 1524
Fragments arranged by size:
1524 939 787 639 597 393 268 155
42
EcoO109I rG'GnC_Cy
Cuts at: 2374 2374
Size: 5344
EcoRI G'AATT_C
Cuts at: 2653 3031 2653
Size: 378 4966
FspI TGC'GCA
Cuts at: 3850 3850
Size: 5344
HaeII r_GCGC'y
Cuts at: 1678 2460 4491 1678
Size: 782 2031 2531
Fragments arranged by size:
2531 2031 782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2158 2443 2475 3985
Size: 32 135 32 554 32 285 32 1510
Cuts at: 3985 4017 4059 4091 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1510 554 285 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2155 2688 2934 669
Size: 988 281 217 533 246 3079
Fragments arranged by size:
3079 988 533 281 246 217
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5344
HpaI GTT'AAC
Cuts at: 2934 2934
Size: 5344
KpnI G_GTAC'C
Cuts at: 2669 2669
Size: 5344
MfeI C'AATT_G
Cuts at: 2943 2943
Size: 5344
MluI A'CGCG_T
Cuts at: 5220 5220
Size: 5344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 4458 4642 1305
Size: 834 2319 184 2007
Fragments arranged by size:
2319 2007 834 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2202 2333 10
Size: 54 1425 343 370 131 3021
Fragments arranged by size:
3021 1425 370 343 131 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3302 3661 3820 518
Size: 648 2136 359 159 2042
Fragments arranged by size:
2136 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2760 3437 4585 4830 1606
Size: 1154 677 1148 245 2120
Fragments arranged by size:
2120 1154 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3853
Fragments arranged by size:
3853 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5344
NotI GC'GGCC_GC
Cuts at: 2791 2791
Size: 5344
NspI r_CATG'y
Cuts at: 1132 1229 2702 4247 1132
Size: 97 1473 1545 2229
Fragments arranged by size:
2229 1545 1473 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5344
PciI A'CATG_T
Cuts at: 4243 4243
Size: 5344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2620 1108
Size: 1512 3832
PfoI T'CCnGG_A
Cuts at: 2403 2445 2403
Size: 42 5302
PmeI GTTT'AAAC
Cuts at: 2648 2648
Size: 5344
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2084 2116 2426 2458 2471 2503 3413 3445 4926
Size: 32 310 32 13 32 910 32 1481
Cuts at: 4926 4958 2084
Size: 32 2470
Fragments arranged by size:
2470 1481 910 310 32 32 32 32
32 13
PpuMI rG'GwC_Cy
Cuts at: 2374 2374
Size: 5344
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5344
PsiI TTA'TAA
Cuts at: 2914 2914
Size: 5344
PstI C_TGCA'G
Cuts at: 830 2696 830
Size: 1866 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5344
SalI G'TCGA_C
Cuts at: 1936 2686 1936
Size: 750 4594
SbfI CC_TGCA'GG
Cuts at: 2696 2696
Size: 5344
ScaI AGT'ACT
Cuts at: 3592 5304 3592
Size: 1712 3632
SfcI C'TryA_G
Cuts at: 826 1045 2180 2692 3827 4508 4699 826
Size: 219 1135 512 1135 681 191 1471
Fragments arranged by size:
1471 1135 1135 681 512 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5344
SmaI CCC'GGG
Cuts at: 1872 2147 2671 1872
Size: 275 524 4545
Fragments arranged by size:
4545 524 275
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2492 3448 4349 4611 820
Size: 197 926 549 956 901 262 1553
Fragments arranged by size:
1553 956 926 901 549 262 197
SnaBI TAC'GTA
Cuts at: 493 3135 493
Size: 2642 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5344
SphI G_CATG'C
Cuts at: 1132 2702 1132
Size: 1570 3774
SspI AAT'ATT
Cuts at: 5 52 3268 5
Size: 47 3216 2081
Fragments arranged by size:
3216 2081 47
StuI AGG'CCT
Cuts at: 1517 2411 1517
Size: 894 4450
StyI C'CwwG_G
Cuts at: 513 1065 2004 2783 513
Size: 552 939 779 3074
Fragments arranged by size:
3074 939 779 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2150 2205 3371 3388 3541 3726 4171
Size: 1255 570 55 1166 17 153 185 445
Cuts at: 4171 325
Size: 1498
Fragments arranged by size:
1498 1255 1166 570 445 185 153 55
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3590 5302 96
Size: 274 80 33 51 1443 1613 1712 138
Fragments arranged by size:
1712 1613 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2133 2165 5304 5336 2133
Size: 32 3139 32 2141
Fragments arranged by size:
3139 2141 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5344
XbaI T'CTAG_A
Cuts at: 2680 2680
Size: 5344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5344
XmaI C'CCGG_G
Cuts at: 1870 2145 2669 1870
Size: 275 524 4545
Fragments arranged by size:
4545 524 275
XmnI GAAnn'nnTTC
Cuts at: 2099 3473 2099
Size: 1374 3970
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3152 276
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI