(Linear) (Six-base) MAPSORT of: KIEE3059.seq Check: 8215 from: 1 to: 639 KIEE3059.orf With 182 enzymes: SgfI * March 22, 2012 16:07 .. AfeI AGC'GCT Cuts at: 0 453 639 Size: 453 186 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 466 498 639 Size: 466 32 141 Fragments arranged by size: 466 141 32 AlwNI CAG_nnn'CTG Cuts at: 0 55 639 Size: 55 584 ApoI r'AATT_y Cuts at: 0 28 639 Size: 28 611 AvaI C'yCGr_G Cuts at: 0 140 639 Size: 140 499 BamHI G'GATC_C Cuts at: 0 136 639 Size: 136 503 BanII G_rGCy'C Cuts at: 0 252 553 639 Size: 252 301 86 Fragments arranged by size: 301 252 86 BbsI GAAGACnn'nnnn_ Cuts at: 0 169 639 Size: 169 470 BbvCI CC'TCA_GC Cuts at: 0 623 639 Size: 623 16 Bme1580I G_kGCm'C Cuts at: 0 498 609 639 Size: 498 111 30 Fragments arranged by size: 498 111 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 421 463 639 Size: 266 155 42 176 Fragments arranged by size: 266 176 155 42 Bpu10I CC'TnA_GC Cuts at: 0 380 623 639 Size: 380 243 16 Fragments arranged by size: 380 243 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 639 Size: 472 167 BsaI GGTCTCn'nnnn_ Cuts at: 0 1 439 639 Size: 1 438 200 Fragments arranged by size: 438 200 1 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 466 496 639 Size: 466 30 143 Fragments arranged by size: 466 143 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 490 639 Size: 490 149 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 639 Size: 68 571 BsmI GAATG_Cn' Cuts at: 0 557 639 Size: 557 82 Bsp1286I G_dGCh'C Cuts at: 0 252 498 553 609 639 Size: 252 246 55 56 30 Fragments arranged by size: 252 246 56 55 30 BsrBI CCG'CTC Cuts at: 0 204 384 639 Size: 204 180 255 Fragments arranged by size: 255 204 180 BstXI CCAn_nnnn'nTGG Cuts at: 0 342 639 Size: 342 297 BstYI r'GATC_y Cuts at: 0 136 639 Size: 136 503 BtgI C'CryG_G Cuts at: 0 614 639 Size: 614 25 BtsI GCAGTG_nn' Cuts at: 0 51 639 Size: 51 588 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 107 138 142 173 639 Size: 107 31 4 31 466 Fragments arranged by size: 466 107 31 31 4 EaeI y'GGCC_r Cuts at: 0 195 326 639 Size: 195 131 313 Fragments arranged by size: 313 195 131 EarI CTCTTCn'nnn_ Cuts at: 0 28 639 Size: 28 611 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 571 639 Size: 571 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 421 463 639 Size: 266 155 42 176 Fragments arranged by size: 266 176 155 42 EcoO109I rG'GnC_Cy Cuts at: 0 369 639 Size: 369 270 HaeII r_GCGC'y Cuts at: 0 455 639 Size: 455 184 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 121 153 438 470 639 Size: 121 32 285 32 169 Fragments arranged by size: 285 169 121 32 32 HincII GTy'rAC Cuts at: 0 150 639 Size: 150 489 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 134 639 Size: 134 505 MscI TGG'CCA Cuts at: 0 197 328 639 Size: 197 131 311 Fragments arranged by size: 311 197 131 PflMI CCAn_nnn'nTGG Cuts at: 0 615 639 Size: 615 24 PfoI T'CCnGG_A Cuts at: 0 398 440 639 Size: 398 42 199 Fragments arranged by size: 398 199 42 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 79 111 421 453 466 498 639 Size: 79 32 310 32 13 32 141 Fragments arranged by size: 310 141 79 32 32 32 13 PpuMI rG'GwC_Cy Cuts at: 0 369 639 Size: 369 270 SfcI C'TryA_G Cuts at: 0 175 639 Size: 175 464 SmaI CCC'GGG Cuts at: 0 142 639 Size: 142 497 SmlI C'TyrA_G Cuts at: 0 487 639 Size: 487 152 StuI AGG'CCT Cuts at: 0 406 639 Size: 406 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 145 200 639 Size: 145 55 439 Fragments arranged by size: 439 145 55 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 128 160 639 Size: 128 32 479 Fragments arranged by size: 479 128 32 XmaI C'CCGG_G Cuts at: 0 140 639 Size: 140 499 XmnI GAAnn'nnTTC Cuts at: 0 94 639 Size: 94 545 Enzymes that do cut: AfeI AloI AlwNI ApoI AvaI BamHI BanII BbsI BbvCI Bme1580I BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsgI BsmI Bsp1286I BsrBI BstXI BstYI BtgI BtsI CspCI EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII MmeI MscI PflMI PfoI PpiI PpuMI SfcI SmaI SmlI StuI TaqII TstI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI Tth111I XbaI XcmI XhoI ZraI