################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AscI AseI AsnI Asp718I AviII AvrII BbrPI BcgI BciVI BfrI BglII BlnI BsaAI BsaBI BseAI BsmI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstXI BstZ17I ClaI CpoI DraIII EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NotI NruI NsiI PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SexAI SfiI SgrAI SnaBI SpeI SspI StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 495 GACGTC GACGT^C AflIII 1769 ACGTGT A^C[GA][CT]GT AgeI 2505 ACCGGT A^CCGGT AhdI 2494 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1948 CCTGAGG CC^T[ACGT]AGG Asp700I 2804 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2494 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 175 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 159 GGATCC G^GATCC BbvCI 828 CCTCAGC CCTCAGC(-5/-2) BclI 842 TGATCA T^GATCA BfaI 118 CTAG C^TAG BglI 1093 GCCCGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC Bsp1407I 2186 TGTACA T^GTACA BspHI 2120 TCATGA T^CATGA BsrDI 5 GCAATG GCAATG(2/0) BsrGI 2186 TGTACA T^GTACA BssSI 378 CACGAG CACGAG(-5/-1) BstBI 2907 TTCGAA TT^CGAA Bsu36I 1948 CCTGAGG CC^T[ACGT]AGG DraI 270 TTTAAA TTT^AAA EagI 1646 CGGCCG C^GGCCG Eam1105I 2494 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1646 CGGCCG C^GGCCG Eco52I 1646 CGGCCG C^GGCCG Eco81I 1948 CCTGAGG CC^T[ACGT]AGG FbaI 842 TGATCA T^GATCA HincII 1813 GTCAAC GT[CT]^[GA]AC HindII 1813 GTCAAC GT[CT]^[GA]AC KspI 1334 CCGCGG CCGC^GG MaeI 118 CTAG C^TAG NcoI 1778 CCATGG C^CATGG NheI 117 GCTAGC G^CTAGC NspV 2907 TTCGAA TT^CGAA PflFI 175 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2505 ACCGGT A^CCGGT PvuII 396 CAGCTG CAG^CTG RcaI 2120 TCATGA T^CATGA SacII 1334 CCGCGG CCGC^GG SbfI 2562 CCTGCAGG CCTGCA^GG SfuI 2907 TTCGAA TT^CGAA SmaI 1620 CCCGGG CCC^GGG SphI 2204 GCATGC GCATG^C Sse8387I 2562 CCTGCAGG CCTGCA^GG SspBI 2186 TGTACA T^GTACA Tth111I 175 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1620 CCCGGG C^CCGGG XmaI 1620 CCCGGG C^CCGGG XmnI 2804 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcsI 1441 AAATTT [GA]^AATT[CT] 2737 AAATTC Alw44I 652 GTGCAC G^TGCAC 2644 GTGCAC AlwNI 934 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 2685 CAGCCACTG Aor51HI 737 AGCGCT AGC^GCT 2104 AGCGCT ApaLI 652 GTGCAC G^TGCAC 2644 GTGCAC ApoI 1441 AAATTT [GA]^AATT[CT] 2737 AAATTC BlpI 257 GCTTAGC GC^T[ACGT]AGC 2100 GCTGAGC Bpu1102I 257 GCTTAGC GC^T[ACGT]AGC 2100 GCTGAGC BsaI 1833 GAGACC GGTCTC(1/5) 2593 GAGACC BsiEI 1646 CGGCCG CG[GA][CT]^CG 2259 CGACCG BsiWI 626 CGTACG C^GTACG 1028 CGTACG BstEII 1173 GGTCACC G^GT[ACGT]ACC 2031 GGTCACC BstPI 1173 GGTCACC G^GT[ACGT]ACC 2031 GGTCACC CelII 257 GCTTAGC GC^T[ACGT]AGC 2100 GCTGAGC DrdI 826 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2418 GACGCCCAAGTC Eco47III 737 AGCGCT AGC^GCT 2104 AGCGCT EcoO65I 1173 GGTCACC G^GT[ACGT]ACC 2031 GGTCACC MseI 271 TTAA T^TAA 2299 TTAA NspI 791 GCATGT [GA]CATG^[CT] 2204 GCATGC PaeR7I 143 CTCGAG C^TCGAG 2580 CTCGAG SacI 321 GAGCTC GAGCT^C 1854 GAGCTC SapI 1851 GAAGAGC GCTCTTC(1/4) 134 GCTCTTC TfiI 2341 GATTC G^A[AT]TC 2886 GATTC Tru9I 271 TTAA T^TAA 2299 TTAA Tsp509I 1442 AATT ^AATT 2738 AATT XhoI 143 CTCGAG C^TCGAG 2580 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII