(Circular) (Six-base) MAPSORT of: pFN21AB9645.seq Check: 7456 from: 1 to: 7801 pFN21AB9645 7801 bp With 182 enzymes: SgfI * March 28, 2012 12:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2363 3658 2363 Size: 1295 6506 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2504 5611 278 Size: 53 83 186 936 968 3107 2468 Fragments arranged by size: 3107 2468 968 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5144 1937 Size: 3207 4594 Acc65I G'GTAC_C Cuts at: 5122 5122 Size: 7801 AclI AA'CG_TT Cuts at: 1563 5928 6301 1563 Size: 4365 373 3063 Fragments arranged by size: 4365 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 2810 2900 2921 3378 4837 4880 5864 Size: 454 724 90 21 457 1459 43 984 Cuts at: 5864 7248 7516 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1459 1384 984 724 457 454 268 90 43 21 AfeI AGC'GCT Cuts at: 2744 4111 2744 Size: 1367 6434 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7604 AflIII A'CryG_T Cuts at: 1203 3774 6700 7677 1203 Size: 2571 2926 977 1327 Fragments arranged by size: 2926 2571 1327 977 AgeI A'CCGG_T Cuts at: 4510 6632 4510 Size: 2122 5679 AhdI GACnn_n'nnGTC Cuts at: 4504 6530 4504 Size: 2026 5775 AleI CACnn'nnGTG Cuts at: 1166 2642 2682 4241 1166 Size: 1476 40 1559 4726 Fragments arranged by size: 4726 1559 1476 40 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2490 4226 4272 1184 Size: 1306 1736 46 4713 Fragments arranged by size: 4713 1736 1306 46 AlwNI CAG_nnn'CTG Cuts at: 2944 4695 5544 7116 2944 Size: 1751 849 1572 3629 Fragments arranged by size: 3629 1751 1572 849 ApaI G_GGCC'C Cuts at: 3953 4637 4943 3953 Size: 684 306 6811 Fragments arranged by size: 6811 684 306 ApaLI G'TGCA_C Cuts at: 2657 4649 5858 7014 2657 Size: 1992 1209 1156 3444 Fragments arranged by size: 3444 1992 1209 1156 ApoI r'AATT_y Cuts at: 1493 3446 4742 5110 5336 5488 7597 1493 Size: 1953 1296 368 226 152 2109 1697 Fragments arranged by size: 2109 1953 1697 1296 368 226 152 AseI AT'TA_AT Cuts at: 160 1032 6355 160 Size: 872 5323 1606 Fragments arranged by size: 5323 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7801 AvaI C'yCGr_G Cuts at: 1870 1943 2148 3625 4585 5060 5126 1870 Size: 73 205 1477 960 475 66 4545 Fragments arranged by size: 4545 1477 960 475 205 73 66 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2413 2446 2413 Size: 33 7768 BamHI G'GATC_C Cuts at: 2164 5131 5498 2164 Size: 2967 367 4467 Fragments arranged by size: 4467 2967 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2466 2594 2947 3071 3629 Size: 325 217 627 679 128 353 124 558 Cuts at: 3629 5122 6577 618 Size: 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 679 627 558 353 325 217 128 124 BanII G_rGCy'C Cuts at: 1391 2330 2529 3456 3863 3953 4637 4674 4943 Size: 939 199 927 407 90 684 37 269 Cuts at: 4943 4973 5120 1391 Size: 30 147 4072 Fragments arranged by size: 4072 939 927 684 407 269 199 147 90 37 30 BbeI G_GCGC'C Cuts at: 2598 2951 3075 2598 Size: 353 124 7324 Fragments arranged by size: 7324 353 124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2578 2720 3699 4968 928 Size: 971 679 142 979 1269 3761 Fragments arranged by size: 3761 1269 979 971 679 142 BbvCI CC'TCA_GC Cuts at: 2834 2834 Size: 7801 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5992 6026 1903 Size: 34 4055 34 3678 Fragments arranged by size: 4055 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5693 6903 5693 Size: 1210 6591 BclI T'GATC_A Cuts at: 1548 2847 1548 Size: 1299 6502 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3104 6412 136 Size: 107 122 71 631 605 1432 3308 1525 Fragments arranged by size: 3308 1525 1432 631 605 122 107 71 BglII A'GATC_T Cuts at: 7796 7796 Size: 7801 BlpI GC'TnA_GC Cuts at: 2263 4106 5218 2263 Size: 1843 1112 4846 Fragments arranged by size: 4846 1843 1112 Bme1580I G_kGCm'C Cuts at: 1790 2661 3189 3632 3953 4637 4653 4943 5862 Size: 871 528 443 321 684 16 290 919 Cuts at: 5862 7018 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 919 871 684 528 443 321 290 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7801 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2051 3975 4570 Size: 416 53 83 399 24 634 1924 595 Cuts at: 4570 4677 4955 5532 6490 442 Size: 107 278 577 958 1753 Fragments arranged by size: 1924 1753 958 634 595 577 416 399 278 107 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2126 1056 Size: 1070 6731 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3877 3877 Size: 7801 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2395 3295 3554 6461 1239 Size: 1156 900 259 2907 2579 Fragments arranged by size: 2907 2579 1156 900 259 Bpu10I CC'TnA_GC Cuts at: 2523 2834 2964 2523 Size: 311 130 7360 Fragments arranged by size: 7360 311 130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3101 3509 5926 6791 7089 3101 Size: 408 2417 865 298 3813 Fragments arranged by size: 3813 2417 865 408 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3832 4592 6464 882 Size: 617 2333 760 1872 2219 Fragments arranged by size: 2333 2219 1872 760 617 BsaAI yAC'GTr Cuts at: 493 1204 5592 493 Size: 711 4388 2702 Fragments arranged by size: 4388 2702 711 BsaBI GATnn'nnATC Cuts at: 7795 7795 Size: 7801 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 2501 2595 2948 Size: 53 83 186 936 549 419 94 353 Cuts at: 2948 3072 4424 5608 5990 6625 275 Size: 124 1352 1184 382 635 1451 Fragments arranged by size: 1451 1352 1184 936 635 549 419 382 353 186 124 94 83 53 BsaWI w'CCGG_w Cuts at: 2607 4081 4510 4998 6234 6632 6906 7053 7464 Size: 1474 429 488 1236 398 274 147 411 Cuts at: 7464 2607 Size: 2944 Fragments arranged by size: 2944 1474 1236 488 429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 2225 2255 2690 Size: 30 165 30 633 30 159 30 435 Cuts at: 2690 2720 4955 4985 1178 Size: 30 2235 30 3994 Fragments arranged by size: 3994 2235 633 435 165 159 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3437 3497 3803 3848 4502 4738 1186 Size: 2251 60 306 45 654 236 4249 Fragments arranged by size: 4249 2251 654 306 236 60 45 BseYI C'CCAG_C Cuts at: 2393 2528 3455 3599 7004 7500 2393 Size: 135 927 144 3405 496 2694 Fragments arranged by size: 3405 2694 927 496 144 135 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2460 2567 3677 3686 3692 3716 5015 Size: 573 724 107 1110 9 6 24 1299 Cuts at: 5015 1163 Size: 3949 Fragments arranged by size: 3949 1299 1110 724 573 107 24 9 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3654 4267 5251 6012 7040 1540 Size: 275 186 1653 613 984 761 1028 2301 Fragments arranged by size: 2301 1653 1028 984 761 613 275 186 BsiHKAI G_wGCw'C Cuts at: 2330 2390 2661 3720 3863 4653 5862 5947 7018 Size: 60 271 1059 143 790 1209 85 1071 Cuts at: 7018 2330 Size: 3113 Fragments arranged by size: 3113 1209 1071 1059 790 271 143 85 60 BsiWI C'GTAC_G Cuts at: 2631 3033 2631 Size: 402 7399 BsmI GAATG_Cn' Cuts at: 5312 5405 5312 Size: 93 7708 BsmBI CGTCTCn'nnnn_ Cuts at: 2012 2191 3550 2012 Size: 179 1359 6263 Fragments arranged by size: 6263 1359 179 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2390 2529 2661 3189 3456 3632 Size: 399 540 60 139 132 528 267 176 Cuts at: 3632 3720 3863 3953 4637 4653 4674 4943 4973 Size: 88 143 90 684 16 21 269 30 Cuts at: 4973 5120 5862 5947 7018 1391 Size: 147 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 684 540 528 399 269 267 176 147 143 139 132 90 88 85 60 30 21 16 BspEI T'CCGG_A Cuts at: 7464 7464 Size: 7801 BspHI T'CATG_A Cuts at: 4125 5690 7420 4125 Size: 1565 1730 4506 Fragments arranged by size: 4506 1730 1565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2363 3483 3658 5156 844 Size: 1519 1120 175 1498 3489 Fragments arranged by size: 3489 1519 1498 1120 175 BsrBI CCG'CTC Cuts at: 1394 1823 5688 1394 Size: 429 3865 3507 Fragments arranged by size: 3865 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2017 6296 6470 65 Size: 1183 769 4279 174 1396 Fragments arranged by size: 4279 1396 1183 769 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2084 3304 3646 4222 4510 4530 6445 Size: 110 274 1220 342 576 288 20 1915 Cuts at: 6445 6632 1700 Size: 187 2869 Fragments arranged by size: 2869 1915 1220 576 342 288 274 187 110 20 BsrGI T'GTAC_A Cuts at: 96 4191 96 Size: 4095 3706 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7801 BssSI C'ACGA_G Cuts at: 2383 5861 6873 2383 Size: 3478 1012 3311 Fragments arranged by size: 3478 3311 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2403 2619 3235 3664 794 Size: 1609 216 616 429 4931 Fragments arranged by size: 4931 1609 616 429 216 BstBI TT'CG_AA Cuts at: 4913 6605 4913 Size: 1692 6109 BstEII G'GTnAC_C Cuts at: 1184 3178 4036 1184 Size: 1994 858 4949 Fragments arranged by size: 4949 1994 858 BstYI r'GATC_y Cuts at: 1972 2164 3111 3261 4864 5131 5498 5883 5900 Size: 192 947 150 1603 267 367 385 17 Cuts at: 5900 7352 7438 7450 7796 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1603 1452 947 385 367 346 267 192 150 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3954 3954 Size: 7801 BtgI C'CryG_G Cuts at: 513 1065 3339 3549 3783 5047 513 Size: 552 2274 210 234 1264 3267 Fragments arranged by size: 3267 2274 1264 552 234 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4199 487 Size: 683 56 2973 4089 Fragments arranged by size: 4089 2973 683 56 BtsI GCAGTG_nn' Cuts at: 2177 2629 3641 5317 6111 6131 2177 Size: 452 1012 1676 794 20 3847 Fragments arranged by size: 3847 1676 1012 794 452 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7801 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7766 DraI TTT'AAA Cuts at: 2277 5105 2277 Size: 2828 4973 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2837 4429 6808 809 Size: 507 1521 1592 2379 1802 Fragments arranged by size: 2379 1802 1592 1521 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2481 2979 3552 Size: 54 1425 50 275 18 651 498 573 Cuts at: 3552 3651 3867 4701 5248 6137 7766 8 Size: 99 216 834 547 889 1629 43 Fragments arranged by size: 1629 1425 889 834 651 573 547 498 275 216 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3651 5248 1537 Size: 275 1839 1597 4090 Fragments arranged by size: 4090 1839 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2010 2146 2148 3249 3851 5731 1353 Size: 657 136 2 1101 602 1880 3423 Fragments arranged by size: 3423 1880 1101 657 602 136 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2320 3700 6388 6762 6908 387 Size: 1064 138 731 1380 2688 374 146 1280 Fragments arranged by size: 2688 1380 1280 1064 731 374 146 138 EcoICRI GAG'CTC Cuts at: 2328 3861 2328 Size: 1533 6268 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2395 2810 2900 2921 3295 3378 Size: 393 454 309 415 90 21 374 83 Cuts at: 3378 3554 4837 4880 5864 6461 7248 7516 1239 Size: 176 1283 43 984 597 787 268 1524 Fragments arranged by size: 1524 1283 984 787 597 454 415 393 374 309 268 176 90 83 43 21 EcoNI CCTnn'n_nnAGG Cuts at: 3479 3869 4142 3479 Size: 390 273 7138 Fragments arranged by size: 7138 390 273 EcoO109I rG'GnC_Cy Cuts at: 2830 2941 3487 3949 3950 2830 Size: 111 546 462 1 6681 Fragments arranged by size: 6681 546 462 111 1 EcoRI G'AATT_C Cuts at: 5110 5488 5110 Size: 378 7423 FspI TGC'GCA Cuts at: 6307 6307 Size: 7801 HaeII r_GCGC'y Cuts at: 1678 2598 2746 2936 2951 3075 4113 6948 1678 Size: 920 148 190 15 124 1038 2835 2531 Fragments arranged by size: 2835 2531 1038 920 190 148 124 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2690 2722 4490 Size: 32 135 32 599 32 487 32 1768 Cuts at: 4490 4522 4781 4813 4955 4987 6442 6474 6516 Size: 32 259 32 142 32 1455 32 42 Cuts at: 6516 6548 1373 Size: 32 2626 Fragments arranged by size: 2626 1768 1455 599 487 259 142 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3820 5145 5391 669 Size: 988 281 1882 1325 246 3079 Fragments arranged by size: 3079 1882 1325 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7801 HpaI GTT'AAC Cuts at: 5391 5391 Size: 7801 KasI G'GCGC_C Cuts at: 2594 2947 3071 2594 Size: 353 124 7324 Fragments arranged by size: 7324 353 124 KpnI G_GTAC'C Cuts at: 5126 5126 Size: 7801 MfeI C'AATT_G Cuts at: 5400 5400 Size: 7801 MluI A'CGCG_T Cuts at: 7677 7677 Size: 7801 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4416 4476 4563 4605 5070 6915 7099 1305 Size: 3111 60 87 42 465 1845 184 2007 Fragments arranged by size: 3111 2007 1845 465 184 87 60 42 MscI TGG'CCA Cuts at: 10 64 1489 1832 2483 3869 4703 10 Size: 54 1425 343 651 1386 834 3108 Fragments arranged by size: 3108 1425 1386 834 651 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2642 2682 4241 4981 5759 6118 6277 Size: 648 1476 40 1559 740 778 359 159 Cuts at: 6277 518 Size: 2042 Fragments arranged by size: 2042 1559 1476 778 740 648 359 159 40 MspA1I CmG'CkG Cuts at: 1606 2403 3341 3930 3991 4105 4180 4327 5217 Size: 797 938 589 61 114 75 147 890 Cuts at: 5217 5894 7042 7287 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 938 890 797 677 589 245 147 114 75 61 NaeI GCC'GGC Cuts at: 2086 3306 3648 4532 2086 Size: 1220 342 884 5355 Fragments arranged by size: 5355 1220 884 342 NarI GG'CG_CC Cuts at: 2595 2948 3072 2595 Size: 353 124 7324 Fragments arranged by size: 7324 353 124 NcoI C'CATG_G Cuts at: 513 1065 3783 513 Size: 552 2718 4531 Fragments arranged by size: 4531 2718 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7801 NgoMIV G'CCGG_C Cuts at: 2084 3304 3646 4530 2084 Size: 1220 342 884 5355 Fragments arranged by size: 5355 1220 884 342 NheI G'CTAG_C Cuts at: 1052 2122 1052 Size: 1070 6731 NotI GC'GGCC_GC Cuts at: 5248 5248 Size: 7801 NspI r_CATG'y Cuts at: 1132 1229 2800 4213 5159 6704 1132 Size: 97 1571 1413 946 1545 2229 Fragments arranged by size: 2229 1571 1545 1413 946 97 PasI CC'CwG_GG Cuts at: 1348 3740 1348 Size: 2392 5409 PciI A'CATG_T Cuts at: 6700 6700 Size: 7801 PflMI CCAn_nnn'nTGG Cuts at: 1108 2724 3175 4699 1108 Size: 1616 451 1524 4210 Fragments arranged by size: 4210 1616 1524 451 PmeI GTTT'AAAC Cuts at: 5105 5105 Size: 7801 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5870 5902 7383 7415 5870 Size: 32 1481 32 6256 Fragments arranged by size: 6256 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2830 2941 3487 2830 Size: 111 546 7144 Fragments arranged by size: 7144 546 111 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7801 PsiI TTA'TAA Cuts at: 5371 5371 Size: 7801 PspOMI G'GGCC_C Cuts at: 3949 4633 4939 3949 Size: 684 306 6811 Fragments arranged by size: 6811 684 306 PspXI vC'TCGA_Gb Cuts at: 4585 4585 Size: 7801 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4801 4833 4801 Size: 32 7769 PstI C_TGCA'G Cuts at: 830 2408 2496 2624 3156 4572 5153 830 Size: 1578 88 128 532 1416 581 3478 Fragments arranged by size: 3478 1578 1416 581 532 128 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7801 PvuII CAG'CTG Cuts at: 2403 2403 Size: 7801 SacI G_AGCT'C Cuts at: 2330 3863 2330 Size: 1533 6268 SacII CC_GC'GG Cuts at: 3342 3342 Size: 7801 SalI G'TCGA_C Cuts at: 1936 5143 1936 Size: 3207 4594 SapI GCTCTTCn'nnn_ Cuts at: 2146 3851 2146 Size: 1705 6096 SbfI CC_TGCA'GG Cuts at: 4572 5153 4572 Size: 581 7220 ScaI AGT'ACT Cuts at: 6049 7761 6049 Size: 1712 6089 SfcI C'TryA_G Cuts at: 826 1045 2404 2492 2620 3152 4568 5149 6284 Size: 219 1359 88 128 532 1416 581 1135 Cuts at: 6284 6965 7156 826 Size: 681 191 1471 Fragments arranged by size: 1471 1416 1359 1135 681 581 532 219 191 128 88 SfoI GGC'GCC Cuts at: 2596 2949 3073 2596 Size: 353 124 7324 Fragments arranged by size: 7324 353 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7801 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7801 SmaI CCC'GGG Cuts at: 1872 3627 5128 1872 Size: 1755 1501 4545 Fragments arranged by size: 4545 1755 1501 SmlI C'TyrA_G Cuts at: 820 1017 1943 2148 3116 3524 4585 5905 6806 Size: 197 926 205 968 408 1061 1320 901 Cuts at: 6806 7068 820 Size: 262 1553 Fragments arranged by size: 1553 1320 1061 968 926 901 408 262 205 197 SnaBI TAC'GTA Cuts at: 493 5592 493 Size: 5099 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7801 SphI G_CATG'C Cuts at: 1132 4213 5159 1132 Size: 3081 946 3774 Fragments arranged by size: 3774 3081 946 SspI AAT'ATT Cuts at: 5 52 5725 5 Size: 47 5673 2081 Fragments arranged by size: 5673 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7801 StyI C'CwwG_G Cuts at: 513 1065 3009 3504 3783 3870 5240 513 Size: 552 1944 495 279 87 1370 3074 Fragments arranged by size: 3074 1944 1370 552 495 279 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3305 4148 5828 5845 5998 6183 6628 Size: 1255 1725 843 1680 17 153 185 445 Cuts at: 6628 325 Size: 1498 Fragments arranged by size: 1725 1680 1498 1255 843 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2799 4191 4614 Size: 274 80 33 51 1443 822 1392 423 Cuts at: 4614 6047 7759 96 Size: 1433 1712 138 Fragments arranged by size: 1712 1443 1433 1392 822 423 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2156 2188 4753 4785 7761 7793 2156 Size: 32 2565 32 2976 32 2164 Fragments arranged by size: 2976 2565 2164 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2183 1316 Size: 867 6934 XbaI T'CTAG_A Cuts at: 5137 5137 Size: 7801 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2266 2905 3511 3790 4933 2266 Size: 639 606 279 1143 5134 Fragments arranged by size: 5134 1143 639 606 279 XhoI C'TCGA_G Cuts at: 1943 2148 4585 1943 Size: 205 2437 5159 Fragments arranged by size: 5159 2437 205 XmaI C'CCGG_G Cuts at: 1870 3625 5126 1870 Size: 1755 1501 4545 Fragments arranged by size: 4545 1755 1501 XmnI GAAnn'nnTTC Cuts at: 4813 5930 4813 Size: 1117 6684 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2502 5609 276 Size: 53 83 186 936 968 3107 2468 Fragments arranged by size: 3107 2468 968 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI RsrII SanDI SexAI SfiI SrfI SwaI