(Linear) (Six-base) MAPSORT of: KIBB9645.seq Check: 331 from: 1 to: 3096 KIBB9645.orf With 182 enzymes: SgfI * March 22, 2012 15:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 358 1653 3096 Size: 358 1295 1443 Fragments arranged by size: 1443 1295 358 AatII G_ACGT'C Cuts at: 0 499 3096 Size: 499 2597 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 81 805 895 916 1373 2832 2875 3096 Size: 81 724 90 21 457 1459 43 221 Fragments arranged by size: 1459 724 457 221 90 81 43 21 AfeI AGC'GCT Cuts at: 0 739 2106 3096 Size: 739 1367 990 Fragments arranged by size: 1367 990 739 AflIII A'CryG_T Cuts at: 0 1769 3096 Size: 1769 1327 AgeI A'CCGG_T Cuts at: 0 2505 3096 Size: 2505 591 AhdI GACnn_n'nnGTC Cuts at: 0 2499 3096 Size: 2499 597 AleI CACnn'nnGTG Cuts at: 0 637 677 2236 3096 Size: 637 40 1559 860 Fragments arranged by size: 1559 860 637 40 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 485 2221 2267 3096 Size: 485 1736 46 829 Fragments arranged by size: 1736 829 485 46 AlwNI CAG_nnn'CTG Cuts at: 0 939 2690 3096 Size: 939 1751 406 Fragments arranged by size: 1751 939 406 ApaI G_GGCC'C Cuts at: 0 1948 2632 2938 3096 Size: 1948 684 306 158 Fragments arranged by size: 1948 684 306 158 ApaLI G'TGCA_C Cuts at: 0 652 2644 3096 Size: 652 1992 452 Fragments arranged by size: 1992 652 452 ApoI r'AATT_y Cuts at: 0 1441 2737 3096 Size: 1441 1296 359 Fragments arranged by size: 1441 1296 359 AvaI C'yCGr_G Cuts at: 0 143 1620 2580 3055 3096 Size: 143 1477 960 475 41 Fragments arranged by size: 1477 960 475 143 41 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 408 441 3096 Size: 408 33 2655 Fragments arranged by size: 2655 408 33 BamHI G'GATC_C Cuts at: 0 159 3096 Size: 159 2937 BanI G'GyrC_C Cuts at: 0 461 589 942 1066 1624 3096 Size: 461 128 353 124 558 1472 Fragments arranged by size: 1472 558 461 353 128 124 BanII G_rGCy'C Cuts at: 0 325 524 1451 1858 1948 2632 2669 2938 Size: 325 199 927 407 90 684 37 269 Cuts at: 2938 2968 3096 Size: 30 128 Fragments arranged by size: 927 684 407 325 269 199 128 90 37 30 BbeI G_GCGC'C Cuts at: 0 593 946 1070 3096 Size: 593 353 124 2026 Fragments arranged by size: 2026 593 353 124 BbsI GAAGACnn'nnnn_ Cuts at: 0 573 715 1694 2963 3096 Size: 573 142 979 1269 133 Fragments arranged by size: 1269 979 573 142 133 BbvCI CC'TCA_GC Cuts at: 0 829 3096 Size: 829 2267 BclI T'GATC_A Cuts at: 0 842 3096 Size: 842 2254 BglI GCCn_nnn'nGGC Cuts at: 0 1099 3096 Size: 1099 1997 BlpI GC'TnA_GC Cuts at: 0 258 2101 3096 Size: 258 1843 995 Fragments arranged by size: 1843 995 258 Bme1580I G_kGCm'C Cuts at: 0 656 1184 1627 1948 2632 2648 2938 3096 Size: 656 528 443 321 684 16 290 158 Fragments arranged by size: 684 656 528 443 321 290 158 16 BmrI ACTGGGnnnn_n' Cuts at: 0 46 1970 2565 2672 2950 3096 Size: 46 1924 595 107 278 146 Fragments arranged by size: 1924 595 278 146 107 46 BmtI G_CTAG'C Cuts at: 0 121 3096 Size: 121 2975 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1872 3096 Size: 1872 1224 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 390 1290 1549 3096 Size: 390 900 259 1547 Fragments arranged by size: 1547 900 390 259 Bpu10I CC'TnA_GC Cuts at: 0 518 829 959 3096 Size: 518 311 130 2137 Fragments arranged by size: 2137 518 311 130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1096 1504 3096 Size: 1096 408 1592 Fragments arranged by size: 1592 1096 408 BsaI GGTCTCn'nnnn_ Cuts at: 0 1827 2587 3096 Size: 1827 760 509 Fragments arranged by size: 1827 760 509 BsaHI Gr'CG_yC Cuts at: 0 77 496 590 943 1067 2419 3096 Size: 77 419 94 353 124 1352 677 Fragments arranged by size: 1352 677 419 353 124 94 77 BsaWI w'CCGG_w Cuts at: 0 602 2076 2505 2993 3096 Size: 602 1474 429 488 103 Fragments arranged by size: 1474 602 488 429 103 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 31 61 220 250 685 715 2950 2980 Size: 31 30 159 30 435 30 2235 30 Cuts at: 2980 3096 Size: 116 Fragments arranged by size: 2235 435 159 116 31 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1432 1492 1798 1843 2497 2733 3096 Size: 1432 60 306 45 654 236 363 Fragments arranged by size: 1432 654 363 306 236 60 45 BseYI C'CCAG_C Cuts at: 0 388 523 1450 1594 3096 Size: 388 135 927 144 1502 Fragments arranged by size: 1502 927 388 144 135 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 455 562 1672 1681 1687 1711 3010 3096 Size: 455 107 1110 9 6 24 1299 86 Fragments arranged by size: 1299 1110 455 107 86 24 9 6 BsiEI CG_ry'CG Cuts at: 0 1649 2262 3096 Size: 1649 613 834 Fragments arranged by size: 1649 834 613 BsiHKAI G_wGCw'C Cuts at: 0 325 385 656 1715 1858 2648 3096 Size: 325 60 271 1059 143 790 448 Fragments arranged by size: 1059 790 448 325 271 143 60 BsiWI C'GTAC_G Cuts at: 0 626 1028 3096 Size: 626 402 2068 Fragments arranged by size: 2068 626 402 BsmBI CGTCTCn'nnnn_ Cuts at: 0 7 186 1545 3096 Size: 7 179 1359 1551 Fragments arranged by size: 1551 1359 179 7 Bsp1286I G_dGCh'C Cuts at: 0 325 385 524 656 1184 1451 1627 1715 Size: 325 60 139 132 528 267 176 88 Cuts at: 1715 1858 1948 2632 2648 2669 2938 2968 3096 Size: 143 90 684 16 21 269 30 128 Fragments arranged by size: 684 528 325 269 267 176 143 139 132 128 90 88 60 30 21 16 BspHI T'CATG_A Cuts at: 0 2120 3096 Size: 2120 976 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 358 1478 1653 3096 Size: 358 1120 175 1443 Fragments arranged by size: 1443 1120 358 175 BsrDI GCAATG_nn' Cuts at: 0 12 3096 Size: 12 3084 BsrFI r'CCGG_y Cuts at: 0 79 1299 1641 2217 2505 2525 3096 Size: 79 1220 342 576 288 20 571 Fragments arranged by size: 1220 576 571 342 288 79 20 BsrGI T'GTAC_A Cuts at: 0 2186 3096 Size: 2186 910 BssSI C'ACGA_G Cuts at: 0 378 3096 Size: 378 2718 BstAPI GCAn_nnn'nTGC Cuts at: 0 398 614 1230 1659 3096 Size: 398 216 616 429 1437 Fragments arranged by size: 1437 616 429 398 216 BstBI TT'CG_AA Cuts at: 0 2908 3096 Size: 2908 188 BstEII G'GTnAC_C Cuts at: 0 1173 2031 3096 Size: 1173 858 1065 Fragments arranged by size: 1173 1065 858 BstYI r'GATC_y Cuts at: 0 159 1106 1256 2859 3096 Size: 159 947 150 1603 237 Fragments arranged by size: 1603 947 237 159 150 Bsu36I CC'TnA_GG Cuts at: 0 1949 3096 Size: 1949 1147 BtgI C'CryG_G Cuts at: 0 1334 1544 1778 3042 3096 Size: 1334 210 234 1264 54 Fragments arranged by size: 1334 1264 234 210 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2194 3096 Size: 2194 902 BtsI GCAGTG_nn' Cuts at: 0 172 624 1636 3096 Size: 172 452 1012 1460 Fragments arranged by size: 1460 1012 452 172 DraI TTT'AAA Cuts at: 0 272 3096 Size: 272 2824 DrdI GACnn_nn'nnGTC Cuts at: 0 832 2424 3096 Size: 832 1592 672 Fragments arranged by size: 1592 832 672 EaeI y'GGCC_r Cuts at: 0 476 974 1547 1646 1862 2696 3096 Size: 476 498 573 99 216 834 400 Fragments arranged by size: 834 573 498 476 400 216 99 EagI C'GGCC_G Cuts at: 0 1646 3096 Size: 1646 1450 EarI CTCTTCn'nnn_ Cuts at: 0 5 141 143 1244 1846 3096 Size: 5 136 2 1101 602 1250 Fragments arranged by size: 1250 1101 602 136 5 2 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 315 1695 3096 Size: 315 1380 1401 Fragments arranged by size: 1401 1380 315 EcoICRI GAG'CTC Cuts at: 0 323 1856 3096 Size: 323 1533 1240 Fragments arranged by size: 1533 1240 323 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 81 390 805 895 916 1290 1373 1549 Size: 81 309 415 90 21 374 83 176 Cuts at: 1549 2832 2875 3096 Size: 1283 43 221 Fragments arranged by size: 1283 415 374 309 221 176 90 83 81 43 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 1474 1864 2137 3096 Size: 1474 390 273 959 Fragments arranged by size: 1474 959 390 273 EcoO109I rG'GnC_Cy Cuts at: 0 825 936 1482 1944 1945 3096 Size: 825 111 546 462 1 1151 Fragments arranged by size: 1151 825 546 462 111 1 HaeII r_GCGC'y Cuts at: 0 593 741 931 946 1070 2108 3096 Size: 593 148 190 15 124 1038 988 Fragments arranged by size: 1038 988 593 190 148 124 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 166 198 685 717 2485 2517 2776 2808 Size: 166 32 487 32 1768 32 259 32 Cuts at: 2808 2950 2982 3096 Size: 142 32 114 Fragments arranged by size: 1768 487 259 166 142 114 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1815 3096 Size: 1815 1281 KasI G'GCGC_C Cuts at: 0 589 942 1066 3096 Size: 589 353 124 2030 Fragments arranged by size: 2030 589 353 124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2411 2471 2558 2600 3065 3096 Size: 2411 60 87 42 465 31 Fragments arranged by size: 2411 465 87 60 42 31 MscI TGG'CCA Cuts at: 0 478 1864 2698 3096 Size: 478 1386 834 398 Fragments arranged by size: 1386 834 478 398 MslI CAynn'nnrTG Cuts at: 0 637 677 2236 2976 3096 Size: 637 40 1559 740 120 Fragments arranged by size: 1559 740 637 120 40 MspA1I CmG'CkG Cuts at: 0 398 1336 1925 1986 2100 2175 2322 3096 Size: 398 938 589 61 114 75 147 774 Fragments arranged by size: 938 774 589 398 147 114 75 61 NaeI GCC'GGC Cuts at: 0 81 1301 1643 2527 3096 Size: 81 1220 342 884 569 Fragments arranged by size: 1220 884 569 342 81 NarI GG'CG_CC Cuts at: 0 590 943 1067 3096 Size: 590 353 124 2029 Fragments arranged by size: 2029 590 353 124 NcoI C'CATG_G Cuts at: 0 1778 3096 Size: 1778 1318 NgoMIV G'CCGG_C Cuts at: 0 79 1299 1641 2525 3096 Size: 79 1220 342 884 571 Fragments arranged by size: 1220 884 571 342 79 NheI G'CTAG_C Cuts at: 0 117 3096 Size: 117 2979 NspI r_CATG'y Cuts at: 0 795 2208 3096 Size: 795 1413 888 Fragments arranged by size: 1413 888 795 PasI CC'CwG_GG Cuts at: 0 1735 3096 Size: 1735 1361 PflMI CCAn_nnn'nTGG Cuts at: 0 719 1170 2694 3096 Size: 719 451 1524 402 Fragments arranged by size: 1524 719 451 402 PpuMI rG'GwC_Cy Cuts at: 0 825 936 1482 3096 Size: 825 111 546 1614 Fragments arranged by size: 1614 825 546 111 PspOMI G'GGCC_C Cuts at: 0 1944 2628 2934 3096 Size: 1944 684 306 162 Fragments arranged by size: 1944 684 306 162 PspXI vC'TCGA_Gb Cuts at: 0 2580 3096 Size: 2580 516 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2796 2828 3096 Size: 2796 32 268 Fragments arranged by size: 2796 268 32 PstI C_TGCA'G Cuts at: 0 403 491 619 1151 2567 3096 Size: 403 88 128 532 1416 529 Fragments arranged by size: 1416 532 529 403 128 88 PvuII CAG'CTG Cuts at: 0 398 3096 Size: 398 2698 SacI G_AGCT'C Cuts at: 0 325 1858 3096 Size: 325 1533 1238 Fragments arranged by size: 1533 1238 325 SacII CC_GC'GG Cuts at: 0 1337 3096 Size: 1337 1759 SapI GCTCTTCn'nnn_ Cuts at: 0 141 1846 3096 Size: 141 1705 1250 Fragments arranged by size: 1705 1250 141 SbfI CC_TGCA'GG Cuts at: 0 2567 3096 Size: 2567 529 SfcI C'TryA_G Cuts at: 0 399 487 615 1147 2563 3096 Size: 399 88 128 532 1416 533 Fragments arranged by size: 1416 533 532 399 128 88 SfoI GGC'GCC Cuts at: 0 591 944 1068 3096 Size: 591 353 124 2028 Fragments arranged by size: 2028 591 353 124 SmaI CCC'GGG Cuts at: 0 1622 3096 Size: 1622 1474 SmlI C'TyrA_G Cuts at: 0 143 1111 1519 2580 3096 Size: 143 968 408 1061 516 Fragments arranged by size: 1061 968 516 408 143 SphI G_CATG'C Cuts at: 0 2208 3096 Size: 2208 888 StyI C'CwwG_G Cuts at: 0 1004 1499 1778 1865 3096 Size: 1004 495 279 87 1231 Fragments arranged by size: 1231 1004 495 279 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1300 2143 3096 Size: 1300 843 953 Fragments arranged by size: 1300 953 843 TatI w'GTAC_w Cuts at: 0 794 2186 2609 3096 Size: 794 1392 423 487 Fragments arranged by size: 1392 794 487 423 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 151 183 2748 2780 3096 Size: 151 32 2565 32 316 Fragments arranged by size: 2565 316 151 32 32 Tth111I GACn'n_nGTC Cuts at: 0 178 3096 Size: 178 2918 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 261 900 1506 1785 2928 3096 Size: 261 639 606 279 1143 168 Fragments arranged by size: 1143 639 606 279 261 168 XhoI C'TCGA_G Cuts at: 0 143 2580 3096 Size: 143 2437 516 Fragments arranged by size: 2437 516 143 XmaI C'CCGG_G Cuts at: 0 1620 3096 Size: 1620 1476 XmnI GAAnn'nnTTC Cuts at: 0 2808 3096 Size: 2808 288 ZraI GAC'GTC Cuts at: 0 497 3096 Size: 497 2599 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI PpuMI PspOMI PspXI PsrI PstI PvuII SacI SacII SapI SbfI SfcI SfoI SmaI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AloI AscI AseI AsiSI AvrII BcgI BciVI BfrBI BglII BmgBI BsaAI BsaBI BsmI BspEI BsrBI BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PvuI RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI XbaI