################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI AocI Aor51HI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BsiWI BspDI BspHI BspLU11I BsrDI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MflI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI SwaI TfiI Tth111I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 1374 TCCGGA T^CCGGA AcsI 727 GAATTC [GA]^AATT[CT] AcyI 1049 GGCGCC G[GA]^CG[CT]C Alw44I 1427 GTGCAC G^TGCAC ApaLI 1427 GTGCAC G^TGCAC ApoI 727 GAATTC [GA]^AATT[CT] AscI 48 GGCGCGCC GG^CGCGCC Asp700I 723 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 1049 GGCGCC GGCGC^C BbsI 400 GAAGAC GAAGAC(2/6) BcgI 87 CGATCCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 1606 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1606 GCTCAGC GC^T[ACGT]AGC BpuAI 400 GAAGAC GAAGAC(2/6) BsaHI 1049 GGCGCC G[GA]^CG[CT]C BseAI 1374 TCCGGA T^CCGGA BsmAI 22 GAGAC GTCTC(1/5) BsmBI 22 GAGACG CGTCTC(1/5) BsmI 1475 GCATTC GAATGC(1/-1) Bsp1407I 1586 TGTACA T^GTACA BspEI 1374 TCCGGA T^CCGGA BspMI 905 ACCTGC ACCTGC(4/8) BsrBI 586 GAGCGG CCGCTC(-3/-3) BsrGI 1586 TGTACA T^GTACA BssHII 49 GCGCGC G^CGCGC BssSI 853 CTCGTG CACGAG(-5/-1) BstAPI 72 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1606 GCTCAGC GC^T[ACGT]AGC DraIII 1258 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 727 GAATTC G^AATTC HaeII 1049 GGCGCC [GA]GCGC^[CT] Hin1I 1049 GGCGCC G[GA]^CG[CT]C KasI 1049 GGCGCC G^GCGCC KspI 549 CCGCGG CCGC^GG MroI 1374 TCCGGA T^CCGGA MseI 686 TTAA T^TAA NarI 1049 GGCGCC GG^CGCC NcoI 1451 CCATGG C^CATGG SacI 1009 GAGCTC GAGCT^C SacII 549 CCGCGG CCGC^GG SfoI 1049 GGCGCC GGC^GCC SphI 1103 GCATGC GCATG^C SspBI 1586 TGTACA T^GTACA StuI 542 AGGCCT AGG^CCT TaqI 704 TCGA T^CGA Tru9I 686 TTAA T^TAA Tsp45I 835 GTGAC ^GT[GC]AC Tsp509I 728 AATT ^AATT TthHB8I 704 TCGA T^CGA XcmI 1053 CCAGAGCTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 723 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 88 GATCC GGATC(4/5) 1481 GGATC BalI 794 TGGCCA TGG^CCA 1448 TGGCCA BsaAI 628 CACGTA [CT]AC^GT[GA] 826 TACGTG BsaWI 1374 TCCGGA [AT]^CCGG[AT] 1702 ACCGGA BsrFI 1046 GCCGGC [GA]^CCGG[CT] 1614 GCCGGC BstXI 566 CCAGGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1053 CCAGAGCTTTGG Cfr10I 1046 GCCGGC [GA]^CCGG[CT] 1614 GCCGGC EcoNI 405 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 852 CCTCGTGGAGG EcoT14I 893 CCAAGG C^C[AT][AT]GG 1451 CCATGG HgaI 24 GACGC GACGC(5/10) 274 GACGC MaeIII 835 GTGAC ^GT[ACGT]AC 998 GTAAC MluNI 794 TGGCCA TGG^CCA 1448 TGGCCA MscI 794 TGGCCA TGG^CCA 1448 TGGCCA MslI 1554 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1560 CATGTCGGTG NaeI 1046 GCCGGC GCC^GGC 1614 GCCGGC NgoMIV 1046 GCCGGC G^CCGGC 1614 GCCGGC NspI 1103 GCATGC [GA]CATG^[CT] 1559 GCATGT PflMI 54 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1257 CCACCCTGTGG PpuMI 710 GGGACCC [GA]G^G[AT]CC[CT] 803 AGGTCCT StyI 893 CCAAGG C^C[AT][AT]GG 1451 CCATGG Van91I 54 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1257 CCACCCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI TseI TspRI XmaCI XmaI