(Circular) (Six-base) MAPSORT of: pFN21AB7806.seq Check: 1002 from: 1 to: 6484 pFN21AB7806 6484 bp With 182 enzymes: SgfI * March 28, 2012 12:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4547 5931 6199 1632 Size: 2915 1384 268 1917 Fragments arranged by size: 2915 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 5383 6360 1203 Size: 4180 977 1327 Fragments arranged by size: 4180 1327 977 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6484 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2732 2764 2732 Size: 32 6452 AlwNI CAG_nnn'CTG Cuts at: 2065 2083 2495 3233 3290 4227 5799 2065 Size: 18 412 738 57 937 1572 2750 Fragments arranged by size: 2750 1572 937 738 412 57 18 ApaI G_GGCC'C Cuts at: 2122 2259 2486 2122 Size: 137 227 6120 Fragments arranged by size: 6120 227 137 ApaLI G'TGCA_C Cuts at: 3432 4541 5697 3432 Size: 1109 1156 4219 Fragments arranged by size: 4219 1156 1109 ApoI r'AATT_y Cuts at: 1493 2732 3793 4019 4171 6280 1493 Size: 1239 1061 226 152 2109 1697 Fragments arranged by size: 2109 1697 1239 1061 226 152 AscI GG'CGCG_CC Cuts at: 2054 2054 Size: 6484 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 2121 2150 2286 2309 2411 3809 1870 Size: 73 178 29 136 23 102 1398 4545 Fragments arranged by size: 4545 1398 178 136 102 73 29 23 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2507 3054 3539 3805 5260 Size: 325 217 627 720 547 485 266 1455 Cuts at: 5260 618 Size: 1842 Fragments arranged by size: 1842 1455 720 627 547 485 325 266 217 BanII G_rGCy'C Cuts at: 1391 2122 2151 2176 2259 2306 2486 3018 3803 Size: 731 29 25 83 47 180 532 785 Cuts at: 3803 1391 Size: 4072 Fragments arranged by size: 4072 785 731 532 180 83 47 29 25 BbeI G_GCGC'C Cuts at: 3058 3058 Size: 6484 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2412 928 Size: 971 513 5000 Fragments arranged by size: 5000 971 513 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2081 2115 4675 4709 1903 Size: 34 144 34 2560 34 3678 Fragments arranged by size: 3678 2560 144 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4376 5586 4376 Size: 1210 5274 BclI T'GATC_A Cuts at: 1548 1548 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2189 2201 2241 Size: 107 122 71 631 605 517 12 40 Cuts at: 2241 2490 3401 3461 3739 5095 136 Size: 249 911 60 278 1356 1525 Fragments arranged by size: 1525 1356 911 631 605 517 278 249 122 107 71 60 40 12 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 3612 3901 3612 Size: 289 6195 Bme1580I G_kGCm'C Cuts at: 1790 2122 2161 2259 2486 2510 3103 3436 3542 Size: 332 39 98 227 24 593 333 106 Cuts at: 3542 4545 5701 1790 Size: 1003 1156 2573 Fragments arranged by size: 2573 1156 1003 593 333 332 227 106 98 39 24 BmgBI CAC'GTC Cuts at: 1318 2919 1318 Size: 1601 4883 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4215 5173 442 Size: 416 53 83 399 24 2798 958 1753 Fragments arranged by size: 2798 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3226 3226 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 3002 3383 3422 5144 1239 Size: 848 915 381 39 1722 2579 Fragments arranged by size: 2579 1722 915 848 381 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4609 5474 5772 4609 Size: 865 298 5321 Fragments arranged by size: 5321 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5147 882 Size: 617 3648 2219 Fragments arranged by size: 3648 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2635 2833 4275 493 Size: 711 1431 198 1442 2702 Fragments arranged by size: 2702 1442 1431 711 198 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3055 4291 4673 5308 Size: 53 83 186 936 1522 1236 382 635 Cuts at: 5308 275 Size: 1451 Fragments arranged by size: 1522 1451 1236 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3379 3707 4917 5315 5589 5736 6147 3379 Size: 328 1210 398 274 147 411 3716 Fragments arranged by size: 3716 1210 411 398 328 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2048 2342 2372 3155 Size: 30 165 30 615 30 294 30 783 Cuts at: 3155 3185 3420 3450 3625 3655 1178 Size: 30 235 30 175 30 4007 Fragments arranged by size: 4007 783 615 294 235 175 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2288 2351 2402 2405 2408 2438 2441 Size: 1099 3 63 51 3 3 30 3 Cuts at: 2441 2444 2447 2450 2468 2471 2474 2477 2486 Size: 3 3 3 18 3 3 3 9 Cuts at: 2486 2696 3106 3218 1186 Size: 210 410 112 4452 Fragments arranged by size: 4452 1099 410 210 112 63 51 30 18 9 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2227 2479 2535 2578 3465 3728 5687 6183 2227 Size: 252 56 43 887 263 1959 496 2528 Fragments arranged by size: 2528 1959 887 496 263 252 56 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2088 2952 3309 1163 Size: 573 352 864 357 4338 Fragments arranged by size: 4338 864 573 357 352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2116 2597 3694 3934 4695 5723 Size: 275 186 115 481 1097 240 761 1028 Cuts at: 5723 1540 Size: 2301 Fragments arranged by size: 2301 1097 1028 761 481 275 240 186 115 BsiHKAI G_wGCw'C Cuts at: 2343 3018 3436 4545 4630 5701 2343 Size: 675 418 1109 85 1071 3126 Fragments arranged by size: 3126 1109 1071 675 418 85 BsmI GAATG_Cn' Cuts at: 3480 3995 4088 3480 Size: 515 93 5876 Fragments arranged by size: 5876 515 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2021 2021 Size: 6484 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2151 2161 2176 2259 2306 2343 Size: 399 332 29 10 15 83 47 37 Cuts at: 2343 2486 2510 3018 3103 3436 3542 3803 4545 Size: 143 24 508 85 333 106 261 742 Cuts at: 4545 4630 5701 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 508 399 333 332 261 143 106 85 85 83 47 37 29 24 15 10 BspEI T'CCGG_A Cuts at: 3379 6147 3379 Size: 2768 3716 BspHI T'CATG_A Cuts at: 4373 6103 4373 Size: 1730 4754 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2919 3839 844 Size: 2075 920 3489 Fragments arranged by size: 3489 2075 920 BsrBI CCG'CTC Cuts at: 1394 1823 2593 4371 1394 Size: 429 770 1778 3507 Fragments arranged by size: 3507 1778 770 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4979 5153 65 Size: 1183 3731 174 1396 Fragments arranged by size: 3731 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3051 3619 5128 5315 1700 Size: 110 1241 568 1509 187 2869 Fragments arranged by size: 2869 1509 1241 568 187 110 BsrGI T'GTAC_A Cuts at: 96 3591 96 Size: 3495 2989 BssHII G'CGCG_C Cuts at: 1925 2054 1925 Size: 129 6355 BssSI C'ACGA_G Cuts at: 2858 4544 5556 2858 Size: 1686 1012 3786 Fragments arranged by size: 3786 1686 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2083 794 Size: 1289 5195 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstXI CCAn_nnnn'nTGG Cuts at: 2578 3065 2578 Size: 487 5997 BstYI r'GATC_y Cuts at: 1972 3814 4181 4566 4583 6035 6121 6133 6479 Size: 1842 367 385 17 1452 86 12 346 Cuts at: 6479 1972 Size: 1977 Fragments arranged by size: 1977 1842 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2511 2554 2662 3456 513 Size: 552 1446 43 108 794 3541 Fragments arranged by size: 3541 1446 794 552 108 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2574 487 Size: 683 56 1348 4397 Fragments arranged by size: 4397 1348 683 56 BtsI GCAGTG_nn' Cuts at: 4000 4794 4814 4000 Size: 794 20 5670 Fragments arranged by size: 5670 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DraIII CAC_nnn'GTG Cuts at: 3268 3268 Size: 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 2181 2594 Size: 54 1425 50 275 18 283 68 413 Cuts at: 2594 2799 3150 3235 3402 3453 3691 3721 3931 Size: 205 351 85 167 51 238 30 210 Cuts at: 3931 4820 6449 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 413 351 283 275 238 210 205 167 85 68 54 51 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2113 2594 3691 3931 1537 Size: 275 301 481 1097 240 4090 Fragments arranged by size: 4090 1097 481 301 275 240 EarI CTCTTCn'nnn_ Cuts at: 1353 4414 1353 Size: 3061 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5071 5445 5591 387 Size: 1064 138 3482 374 146 1280 Fragments arranged by size: 3482 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3016 3016 Size: 6484 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 3002 3383 3422 4547 5144 5931 Size: 393 455 915 381 39 1125 597 787 Cuts at: 5931 6199 1239 Size: 268 1524 Fragments arranged by size: 1524 1125 915 787 597 455 393 381 268 39 EcoNI CCTnn'n_nnAGG Cuts at: 2414 2861 2414 Size: 447 6037 EcoO109I rG'GnC_Cy Cuts at: 2256 2483 2492 2600 2716 2809 2902 2995 2256 Size: 227 9 108 116 93 93 93 5745 Fragments arranged by size: 5745 227 116 108 93 93 93 9 EcoRI G'AATT_C Cuts at: 2732 3793 4171 2732 Size: 1061 378 5045 Fragments arranged by size: 5045 1061 378 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 3058 5631 1678 Size: 1380 2573 2531 Fragments arranged by size: 2573 2531 1380 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2342 2374 5125 5157 5199 Size: 32 135 32 770 32 2751 32 42 Cuts at: 5199 5231 1373 Size: 32 2626 Fragments arranged by size: 2751 2626 770 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3828 4074 669 Size: 988 281 1890 246 3079 Fragments arranged by size: 3079 1890 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KasI G'GCGC_C Cuts at: 3054 3054 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2385 5598 5782 1305 Size: 1080 3213 184 2007 Fragments arranged by size: 3213 2007 1080 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2801 3455 10 Size: 54 1425 343 969 654 3039 Fragments arranged by size: 3039 1425 969 654 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3563 3569 4442 4801 4960 518 Size: 648 2397 6 873 359 159 2042 Fragments arranged by size: 2397 2042 873 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2041 2050 2083 2170 2210 2556 2647 3290 Size: 435 9 33 87 40 346 91 643 Cuts at: 3290 3317 3335 3349 3469 3717 3737 3900 4577 Size: 27 18 14 120 248 20 163 677 Cuts at: 4577 5725 5970 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 643 435 346 248 245 163 120 91 87 40 33 27 20 18 14 9 NaeI GCC'GGC Cuts at: 3053 3621 3053 Size: 568 5916 NarI GG'CG_CC Cuts at: 3055 3055 Size: 6484 NcoI C'CATG_G Cuts at: 513 1065 3456 513 Size: 552 2391 3541 Fragments arranged by size: 3541 2391 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NgoMIV G'CCGG_C Cuts at: 3051 3619 3051 Size: 568 5916 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 3112 3568 3842 5387 1132 Size: 97 1883 456 274 1545 2229 Fragments arranged by size: 2229 1883 1545 456 274 97 PasI CC'CwG_GG Cuts at: 1348 2571 3142 1348 Size: 1223 571 4690 Fragments arranged by size: 4690 1223 571 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 3268 1108 Size: 957 1203 4324 Fragments arranged by size: 4324 1203 957 PfoI T'CCnGG_A Cuts at: 2736 2736 Size: 6484 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2716 2809 2716 Size: 93 6391 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspOMI G'GGCC_C Cuts at: 2118 2255 2482 2118 Size: 137 227 6120 Fragments arranged by size: 6120 227 137 PstI C_TGCA'G Cuts at: 830 2209 2652 3373 3836 830 Size: 1379 443 721 463 3478 Fragments arranged by size: 3478 1379 721 463 443 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 2083 2210 2647 3290 3317 3335 3349 3469 2083 Size: 127 437 643 27 18 14 120 5098 Fragments arranged by size: 5098 643 437 127 120 27 18 14 SacI G_AGCT'C Cuts at: 3018 3018 Size: 6484 SacII CC_GC'GG Cuts at: 2557 2557 Size: 6484 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SanDI GG'GwC_CC Cuts at: 2716 2716 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SfcI C'TryA_G Cuts at: 826 1045 2012 2205 2648 3369 3832 4967 5648 Size: 219 967 193 443 721 463 1135 681 Cuts at: 5648 5839 826 Size: 191 1471 Fragments arranged by size: 1471 1135 967 721 681 463 443 219 193 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2490 3401 3461 2490 Size: 911 60 5513 Fragments arranged by size: 5513 911 60 SfoI GGC'GCC Cuts at: 3056 3056 Size: 6484 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 2123 2288 2311 3811 1872 Size: 251 165 23 1500 4545 Fragments arranged by size: 4545 1500 251 165 23 SmlI C'TyrA_G Cuts at: 820 1017 1943 4588 5489 5751 820 Size: 197 926 2645 901 262 1553 Fragments arranged by size: 2645 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3112 3842 1132 Size: 1980 730 3774 Fragments arranged by size: 3774 1980 730 SrfI GCCC'GGGC Cuts at: 2123 2123 Size: 6484 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 2549 1517 Size: 1032 5452 StyI C'CwwG_G Cuts at: 513 1065 2898 3456 3923 513 Size: 552 1833 558 467 3074 Fragments arranged by size: 3074 1833 558 552 467 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2241 3136 4511 4528 4681 4866 5311 Size: 1255 661 895 1375 17 153 185 445 Cuts at: 5311 325 Size: 1498 Fragments arranged by size: 1498 1375 1255 895 661 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3591 4730 6442 Size: 274 80 33 51 1443 1614 1139 1712 Cuts at: 6442 96 Size: 138 Fragments arranged by size: 1712 1614 1443 1139 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6444 6476 6444 Size: 32 6452 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3065 3065 Size: 6484 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 2121 2286 2309 3809 1870 Size: 251 165 23 1500 4545 Fragments arranged by size: 4545 1500 251 165 23 XmnI GAAnn'nnTTC Cuts at: 2732 4613 2732 Size: 1881 4603 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SwaI