(Linear) (Six-base) MAPSORT of: KIBB7806.seq Check: 4958 from: 1 to: 1779 KIBB7806.orf With 182 enzymes: SgfI * March 22, 2012 15:41 .. AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 727 759 1779 Size: 727 32 1020 Fragments arranged by size: 1020 727 32 AlwNI CAG_nnn'CTG Cuts at: 0 60 78 490 1228 1285 1779 Size: 60 18 412 738 57 494 Fragments arranged by size: 738 494 412 60 57 18 ApaI G_GGCC'C Cuts at: 0 117 254 481 1779 Size: 117 137 227 1298 Fragments arranged by size: 1298 227 137 117 ApaLI G'TGCA_C Cuts at: 0 1427 1779 Size: 1427 352 ApoI r'AATT_y Cuts at: 0 727 1779 Size: 727 1052 AscI GG'CGCG_CC Cuts at: 0 49 1779 Size: 49 1730 AvaI C'yCGr_G Cuts at: 0 116 145 281 304 406 1779 Size: 116 29 136 23 102 1373 Fragments arranged by size: 1373 136 116 102 29 23 BanI G'GyrC_C Cuts at: 0 502 1049 1534 1779 Size: 502 547 485 245 Fragments arranged by size: 547 502 485 245 BanII G_rGCy'C Cuts at: 0 117 146 171 254 301 481 1013 1779 Size: 117 29 25 83 47 180 532 766 Fragments arranged by size: 766 532 180 117 83 47 29 25 BbeI G_GCGC'C Cuts at: 0 1053 1779 Size: 1053 726 BbsI GAAGACnn'nnnn_ Cuts at: 0 407 1779 Size: 407 1372 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 76 110 1779 Size: 76 34 1669 Fragments arranged by size: 1669 76 34 BglI GCCn_nnn'nGGC Cuts at: 0 184 196 236 485 1396 1456 1734 1779 Size: 184 12 40 249 911 60 278 45 Fragments arranged by size: 911 278 249 184 60 45 40 12 BlpI GC'TnA_GC Cuts at: 0 1607 1779 Size: 1607 172 Bme1580I G_kGCm'C Cuts at: 0 117 156 254 481 505 1098 1431 1537 Size: 117 39 98 227 24 593 333 106 Cuts at: 1537 1779 Size: 242 Fragments arranged by size: 593 333 242 227 117 106 98 39 24 BmgBI CAC'GTC Cuts at: 0 914 1779 Size: 914 865 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1221 1779 Size: 1221 558 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 997 1378 1417 1779 Size: 82 915 381 39 362 Fragments arranged by size: 915 381 362 82 39 BsaAI yAC'GTr Cuts at: 0 630 828 1779 Size: 630 198 951 Fragments arranged by size: 951 630 198 BsaHI Gr'CG_yC Cuts at: 0 1050 1779 Size: 1050 729 BsaWI w'CCGG_w Cuts at: 0 1374 1702 1779 Size: 1374 328 77 Fragments arranged by size: 1374 328 77 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 13 43 337 367 1150 1180 1415 1445 Size: 13 30 294 30 783 30 235 30 Cuts at: 1445 1620 1650 1779 Size: 175 30 129 Fragments arranged by size: 783 294 235 175 129 30 30 30 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 280 283 346 397 400 403 433 436 Size: 280 3 63 51 3 3 30 3 Cuts at: 436 439 442 445 463 466 469 472 481 Size: 3 3 3 18 3 3 3 9 Cuts at: 481 691 1101 1213 1779 Size: 210 410 112 566 Fragments arranged by size: 566 410 280 210 112 63 51 30 18 9 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 222 474 530 573 1460 1723 1779 Size: 222 252 56 43 887 263 56 Fragments arranged by size: 887 263 252 222 56 56 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 83 947 1304 1779 Size: 83 864 357 475 Fragments arranged by size: 864 475 357 83 BsiEI CG_ry'CG Cuts at: 0 111 592 1689 1779 Size: 111 481 1097 90 Fragments arranged by size: 1097 481 111 90 BsiHKAI G_wGCw'C Cuts at: 0 338 1013 1431 1779 Size: 338 675 418 348 Fragments arranged by size: 675 418 348 338 BsmI GAATG_Cn' Cuts at: 0 1475 1779 Size: 1475 304 BsmBI CGTCTCn'nnnn_ Cuts at: 0 16 1779 Size: 16 1763 Bsp1286I G_dGCh'C Cuts at: 0 117 146 156 171 254 301 338 481 Size: 117 29 10 15 83 47 37 143 Cuts at: 481 505 1013 1098 1431 1537 1779 Size: 24 508 85 333 106 242 Fragments arranged by size: 508 333 242 143 117 106 85 83 47 37 29 24 15 10 BspEI T'CCGG_A Cuts at: 0 1374 1779 Size: 1374 405 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 914 1779 Size: 914 865 BsrBI CCG'CTC Cuts at: 0 588 1779 Size: 588 1191 BsrFI r'CCGG_y Cuts at: 0 1046 1614 1779 Size: 1046 568 165 Fragments arranged by size: 1046 568 165 BsrGI T'GTAC_A Cuts at: 0 1586 1779 Size: 1586 193 BssHII G'CGCG_C Cuts at: 0 49 1779 Size: 49 1730 BssSI C'ACGA_G Cuts at: 0 853 1779 Size: 853 926 BstAPI GCAn_nnn'nTGC Cuts at: 0 78 1779 Size: 78 1701 BstXI CCAn_nnnn'nTGG Cuts at: 0 573 1060 1779 Size: 573 487 719 Fragments arranged by size: 719 573 487 BtgI C'CryG_G Cuts at: 0 506 549 657 1451 1779 Size: 506 43 108 794 328 Fragments arranged by size: 794 506 328 108 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 569 1779 Size: 569 1210 DraIII CAC_nnn'GTG Cuts at: 0 1263 1779 Size: 1263 516 EaeI y'GGCC_r Cuts at: 0 108 176 589 794 1145 1230 1397 1448 Size: 108 68 413 205 351 85 167 51 Cuts at: 1448 1686 1716 1779 Size: 238 30 63 Fragments arranged by size: 413 351 238 205 167 108 85 68 63 51 30 EagI C'GGCC_G Cuts at: 0 108 589 1686 1779 Size: 108 481 1097 93 Fragments arranged by size: 1097 481 108 93 EcoICRI GAG'CTC Cuts at: 0 1011 1779 Size: 1011 768 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 997 1378 1417 1779 Size: 82 915 381 39 362 Fragments arranged by size: 915 381 362 82 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 409 856 1779 Size: 409 447 923 Fragments arranged by size: 923 447 409 EcoO109I rG'GnC_Cy Cuts at: 0 251 478 487 595 711 804 897 990 Size: 251 227 9 108 116 93 93 93 Cuts at: 990 1779 Size: 789 Fragments arranged by size: 789 251 227 116 108 93 93 93 9 EcoRI G'AATT_C Cuts at: 0 727 1779 Size: 727 1052 HaeII r_GCGC'y Cuts at: 0 1053 1779 Size: 1053 726 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 337 369 1779 Size: 337 32 1410 Fragments arranged by size: 1410 337 32 KasI G'GCGC_C Cuts at: 0 1049 1779 Size: 1049 730 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 380 1779 Size: 380 1399 MscI TGG'CCA Cuts at: 0 796 1450 1779 Size: 796 654 329 Fragments arranged by size: 796 654 329 MslI CAynn'nnrTG Cuts at: 0 1558 1564 1779 Size: 1558 6 215 Fragments arranged by size: 1558 215 6 MspA1I CmG'CkG Cuts at: 0 36 45 78 165 205 551 642 1285 Size: 36 9 33 87 40 346 91 643 Cuts at: 1285 1312 1330 1344 1464 1712 1732 1779 Size: 27 18 14 120 248 20 47 Fragments arranged by size: 643 346 248 120 91 87 47 40 36 33 27 20 18 14 9 NaeI GCC'GGC Cuts at: 0 1048 1616 1779 Size: 1048 568 163 Fragments arranged by size: 1048 568 163 NarI GG'CG_CC Cuts at: 0 1050 1779 Size: 1050 729 NcoI C'CATG_G Cuts at: 0 1451 1779 Size: 1451 328 NgoMIV G'CCGG_C Cuts at: 0 1046 1614 1779 Size: 1046 568 165 Fragments arranged by size: 1046 568 165 NspI r_CATG'y Cuts at: 0 1107 1563 1779 Size: 1107 456 216 Fragments arranged by size: 1107 456 216 PasI CC'CwG_GG Cuts at: 0 566 1137 1779 Size: 566 571 642 Fragments arranged by size: 642 571 566 PflMI CCAn_nnn'nTGG Cuts at: 0 60 1263 1779 Size: 60 1203 516 Fragments arranged by size: 1203 516 60 PfoI T'CCnGG_A Cuts at: 0 731 1779 Size: 731 1048 PpuMI rG'GwC_Cy Cuts at: 0 711 804 1779 Size: 711 93 975 Fragments arranged by size: 975 711 93 PspOMI G'GGCC_C Cuts at: 0 113 250 477 1779 Size: 113 137 227 1302 Fragments arranged by size: 1302 227 137 113 PstI C_TGCA'G Cuts at: 0 204 647 1368 1779 Size: 204 443 721 411 Fragments arranged by size: 721 443 411 204 PvuII CAG'CTG Cuts at: 0 78 205 642 1285 1312 1330 1344 1464 Size: 78 127 437 643 27 18 14 120 Cuts at: 1464 1779 Size: 315 Fragments arranged by size: 643 437 315 127 120 78 27 18 14 SacI G_AGCT'C Cuts at: 0 1013 1779 Size: 1013 766 SacII CC_GC'GG Cuts at: 0 552 1779 Size: 552 1227 SanDI GG'GwC_CC Cuts at: 0 711 1779 Size: 711 1068 SfcI C'TryA_G Cuts at: 0 7 200 643 1364 1779 Size: 7 193 443 721 415 Fragments arranged by size: 721 443 415 193 7 SfiI GGCCn_nnn'nGGCC Cuts at: 0 485 1396 1456 1779 Size: 485 911 60 323 Fragments arranged by size: 911 485 323 60 SfoI GGC'GCC Cuts at: 0 1051 1779 Size: 1051 728 SmaI CCC'GGG Cuts at: 0 118 283 306 1779 Size: 118 165 23 1473 Fragments arranged by size: 1473 165 118 23 SphI G_CATG'C Cuts at: 0 1107 1779 Size: 1107 672 SrfI GCCC'GGGC Cuts at: 0 118 1779 Size: 118 1661 StuI AGG'CCT Cuts at: 0 544 1779 Size: 544 1235 StyI C'CwwG_G Cuts at: 0 893 1451 1779 Size: 893 558 328 Fragments arranged by size: 893 558 328 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 236 1131 1779 Size: 236 895 648 Fragments arranged by size: 895 648 236 TatI w'GTAC_w Cuts at: 0 1586 1779 Size: 1586 193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1060 1779 Size: 1060 719 XmaI C'CCGG_G Cuts at: 0 116 281 304 1779 Size: 116 165 23 1475 Fragments arranged by size: 1475 165 116 23 XmnI GAAnn'nnTTC Cuts at: 0 727 1779 Size: 727 1052 Enzymes that do cut: AloI AlwNI ApaI ApaLI ApoI AscI AvaI BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BplI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI BtgI BtgZI DraIII EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI SfiI SfoI SmaI SphI SrfI StuI StyI TaqII TatI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BmrI BmtI Bpu10I BpuEI BsaI BsaBI BsiWI BspHI BsrDI BstBI BstEII BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI EarI EciI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SspI SwaI TstI Tth111I XbaI XhoI ZraI