################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII AccI AclI AgeI AhdI Aor51HI ApaI AscI AspEI AviII AvrII BalI BbeI BbrPI BcgI BclI BglI BglII BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI Bsp1407I BspDI BspHI BsrFI BsrGI BssHII Bst1107I BstZ17I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoT22I FbaI FseI FspI HaeII KasI KspI MfeI MluI MluNI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1578 GGTACC G^GTACC AccIII 553 TCCGGA T^CCGGA AcyI 81 GGCGTC G[GA]^CG[CT]C AflIII 2747 ACATGT A^C[GA][CT]GT Alw44I 993 GTGCAC G^TGCAC ApaLI 993 GTGCAC G^TGCAC AseI 2703 ATTAAT AT^TAAT AsnI 2703 ATTAAT AT^TAAT Asp718I 1578 GGTACC G^GTACC AspI 66 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 150 GGATCC G^GATCC BbvCI 1503 GCTGAGG CCTCAGC(-5/-2) BciVI 1277 GTATCC GTATCC(6/5) BsaHI 81 GGCGTC G[GA]^CG[CT]C BsaI 119 GAGACC GGTCTC(1/5) BseAI 553 TCCGGA T^CCGGA BsgI 2270 CTGCAC GTGCAG(16/14) BsmBI 3275 GAGACG CGTCTC(1/5) BsmI 3087 GCATTC GAATGC(1/-1) BspEI 553 TCCGGA T^CCGGA BspLU11I 2747 ACATGT A^CATGT BsrBI 549 CCGCTC CCGCTC(-3/-3) BsrDI 1876 GCAATG GCAATG(2/0) BssSI 229 CACGAG CACGAG(-5/-1) BstBI 3245 TTCGAA TT^CGAA DraII 1145 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 2599 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 66 GACTCCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 219 CGGCCA [CT]^GGCC[GA] EcoO109I 1145 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 2419 GAATTC G^AATTC EcoRV 755 GATATC GAT^ATC FauI 597 GCGGG CCCGC(4/6) Hin1I 81 GGCGTC G[GA]^CG[CT]C HincII 523 GTTAAC GT[CT]^[GA]AC HindII 523 GTTAAC GT[CT]^[GA]AC HpaI 523 GTTAAC GTT^AAC KpnI 1578 GGTACC GGTAC^C MroI 553 TCCGGA T^CCGGA NdeI 1553 CATATG CA^TATG NspV 3245 TTCGAA TT^CGAA PflFI 66 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1582 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1145 AGGACCT [GA]G^G[AT]CC[CT] PshAI 2351 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2703 ATTAAT AT^TAAT SacI 694 GAGCTC GAGCT^C SapI 696 GCTCTTC GCTCTTC(1/4) ScaI 1489 AGTACT AGT^ACT SfuI 3245 TTCGAA TT^CGAA SspI 2155 AATATT AAT^ATT Tth111I 66 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1582 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflII 711 CTTAAG C^TTAAG 2371 CTTAAG AvaI 310 CTCGGG C^[CT]CG[GA]G 674 CTCGGG BbsI 2376 GTCTTC GAAGAC(2/6) 3455 GTCTTC BfrI 711 CTTAAG C^TTAAG 2371 CTTAAG BpuAI 2376 GTCTTC GAAGAC(2/6) 3455 GTCTTC BsaBI 1300 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2328 GATCCGAATC BsoBI 310 CTCGGG C^[CT]CG[GA]G 674 CTCGGG BspMI 290 ACCTGC ACCTGC(4/8) 2274 ACCTGC BstAPI 237 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3553 GCAGCCTTTGC BstYI 150 GGATCC [GA]^GATC[CT] 2327 AGATCC HgaI 82 GCGTC GACGC(5/10) 168 GCGTC HindIII 439 AAGCTT A^AGCTT 1769 AAGCTT MaeII 275 ACGT A^CGT 3062 ACGT MamI 1300 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2328 GATCCGAATC MflI 150 GGATCC [GA]^GATC[CT] 2327 AGATCC SmlI 711 CTTAAG C^T[CT][GA]AG 2371 CTTAAG SphI 663 GCATGC GCATG^C 705 GCATGC XhoII 150 GGATCC [GA]^GATC[CT] 2327 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI Eco81I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI