(Circular) (Six-base) MAPSORT of: pFN21AE3501.seq Check: 172 from: 1 to: 8371 pFN21AE3501 8371 bp With 182 enzymes: SgfI * March 28, 2012 12:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4288 4288 Size: 8371 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6181 278 Size: 53 83 186 936 4645 2468 Fragments arranged by size: 4645 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5714 1937 Size: 3777 4594 Acc65I G'GTAC_C Cuts at: 3583 5692 3583 Size: 2109 6262 AclI AA'CG_TT Cuts at: 1563 6498 6871 1563 Size: 4935 373 3063 Fragments arranged by size: 4935 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4388 4590 4643 4890 5133 5447 6434 7818 Size: 2756 202 53 247 243 314 987 1384 Cuts at: 7818 8086 1632 Size: 268 1917 Fragments arranged by size: 2756 1917 1384 987 314 268 247 243 202 53 AflII C'TTAA_G Cuts at: 820 1017 2716 4376 820 Size: 197 1699 1660 4815 Fragments arranged by size: 4815 1699 1660 197 AflIII A'CryG_T Cuts at: 1203 4752 7270 8247 1203 Size: 3549 2518 977 1327 Fragments arranged by size: 3549 2518 1327 977 AgeI A'CCGG_T Cuts at: 7202 7202 Size: 8371 AhdI GACnn_n'nnGTC Cuts at: 7100 7100 Size: 8371 AleI CACnn'nnGTG Cuts at: 1166 3638 1166 Size: 2472 5899 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3013 3656 3984 1184 Size: 1829 643 328 5571 Fragments arranged by size: 5571 1829 643 328 AlwNI CAG_nnn'CTG Cuts at: 2251 2752 3371 6114 7686 2251 Size: 501 619 2743 1572 2936 Fragments arranged by size: 2936 2743 1572 619 501 ApaLI G'TGCA_C Cuts at: 2998 6428 7584 2998 Size: 3430 1156 3785 Fragments arranged by size: 3785 3430 1156 ApoI r'AATT_y Cuts at: 1493 3118 3914 4424 4551 5509 5593 5680 5906 Size: 1625 796 510 127 958 84 87 226 Cuts at: 5906 6058 8167 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1625 958 796 510 226 152 127 87 84 AseI AT'TA_AT Cuts at: 160 1032 4709 6925 160 Size: 872 3677 2216 1606 Fragments arranged by size: 3677 2216 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8371 AvaI C'yCGr_G Cuts at: 1870 1943 2315 2679 5696 1870 Size: 73 372 364 3017 4545 Fragments arranged by size: 4545 3017 372 364 73 BamHI G'GATC_C Cuts at: 2155 5701 6068 2155 Size: 3546 367 4458 Fragments arranged by size: 4458 3546 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2329 3422 3583 3624 5370 Size: 325 217 627 542 1093 161 41 1746 Cuts at: 5370 5692 7147 618 Size: 322 1455 1842 Fragments arranged by size: 1842 1746 1455 1093 627 542 325 322 217 161 41 BanII G_rGCy'C Cuts at: 1391 2686 2703 2982 5690 1391 Size: 1295 17 279 2708 4072 Fragments arranged by size: 4072 2708 1295 279 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4374 5453 928 Size: 971 2475 1079 3846 Fragments arranged by size: 3846 2475 1079 971 BbvCI CC'TCA_GC Cuts at: 3509 3509 Size: 8371 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6562 6596 1903 Size: 34 4625 34 3678 Fragments arranged by size: 4625 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3293 6263 7473 3293 Size: 2970 1210 4191 Fragments arranged by size: 4191 2970 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6982 136 Size: 107 122 71 631 605 5310 1525 Fragments arranged by size: 5310 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8366 8366 Size: 8371 BlpI GC'TnA_GC Cuts at: 5788 5788 Size: 8371 Bme1580I G_kGCm'C Cuts at: 1790 2332 3002 3427 6432 7588 1790 Size: 542 670 425 3005 1156 2573 Fragments arranged by size: 3005 2573 1156 670 542 425 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8371 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2501 3012 4242 Size: 416 53 83 399 24 1084 511 1230 Cuts at: 4242 5561 6102 7060 442 Size: 1319 541 958 1753 Fragments arranged by size: 1753 1319 1230 1084 958 541 511 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8371 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2609 3354 3432 3486 5283 7031 1239 Size: 1370 745 78 54 1797 1748 2579 Fragments arranged by size: 2579 1797 1748 1370 745 78 54 Bpu10I CC'TnA_GC Cuts at: 3509 3509 Size: 8371 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6496 7361 7659 6496 Size: 865 298 7208 Fragments arranged by size: 7208 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2118 7034 882 Size: 617 619 4916 2219 Fragments arranged by size: 4916 2219 619 617 BsaAI yAC'GTr Cuts at: 493 1204 6162 493 Size: 711 4958 2702 Fragments arranged by size: 4958 2702 711 BsaBI GATnn'nnATC Cuts at: 3309 4337 8365 3309 Size: 1028 4028 3315 Fragments arranged by size: 4028 3315 1028 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2087 6178 6560 7195 Size: 53 83 186 936 554 4091 382 635 Cuts at: 7195 275 Size: 1451 Fragments arranged by size: 4091 1451 936 635 554 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2127 2192 2558 3053 6804 7202 7476 7623 8034 Size: 65 366 495 3751 398 274 147 411 Cuts at: 8034 2127 Size: 2464 Fragments arranged by size: 3751 2464 495 411 398 366 274 147 65 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4228 4258 4282 4312 4707 Size: 30 165 30 2825 30 24 30 395 Cuts at: 4707 4737 1178 Size: 30 4812 Fragments arranged by size: 4812 2825 395 165 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2040 2276 3669 4133 5618 1186 Size: 854 236 1393 464 1485 3939 Fragments arranged by size: 3939 1485 1393 854 464 236 BseYI C'CCAG_C Cuts at: 2251 5316 7574 8070 2251 Size: 3065 2258 496 2552 Fragments arranged by size: 3065 2552 2258 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4260 1163 Size: 573 2524 5274 Fragments arranged by size: 5274 2524 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5821 6582 7610 1540 Size: 275 186 3820 761 1028 2301 Fragments arranged by size: 3820 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2703 3002 4018 6432 6517 7588 2703 Size: 299 1016 2414 85 1071 3486 Fragments arranged by size: 3486 2414 1071 1016 299 85 BsmI GAATG_Cn' Cuts at: 5092 5882 5975 5092 Size: 790 93 7488 Fragments arranged by size: 7488 790 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5274 5274 Size: 8371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2332 2686 2703 2982 3002 3427 4018 Size: 399 542 354 17 279 20 425 591 Cuts at: 4018 5690 6432 6517 7588 1391 Size: 1672 742 85 1071 2174 Fragments arranged by size: 2174 1672 1071 742 591 542 425 399 354 279 85 20 17 BspEI T'CCGG_A Cuts at: 2558 8034 2558 Size: 5476 2895 BspHI T'CATG_A Cuts at: 6260 7990 6260 Size: 1730 6641 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2304 4288 5726 844 Size: 1460 1984 1438 3489 Fragments arranged by size: 3489 1984 1460 1438 BsrBI CCG'CTC Cuts at: 1394 1823 2556 6258 1394 Size: 429 733 3702 3507 Fragments arranged by size: 3702 3507 733 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3888 6866 7040 65 Size: 1183 2640 2978 174 1396 Fragments arranged by size: 2978 2640 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7015 7202 1700 Size: 110 5205 187 2869 Fragments arranged by size: 5205 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8371 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8371 BssSI C'ACGA_G Cuts at: 2234 6431 7443 2234 Size: 4197 1012 3162 Fragments arranged by size: 4197 3162 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2248 5564 794 Size: 1454 3316 3601 Fragments arranged by size: 3601 3316 1454 BstBI TT'CG_AA Cuts at: 5251 7175 5251 Size: 1924 6447 BstEII G'GTnAC_C Cuts at: 1184 2266 2495 4369 5114 1184 Size: 1082 229 1874 745 4441 Fragments arranged by size: 4441 1874 1082 745 229 BstXI CCAn_nnnn'nTGG Cuts at: 2882 4031 4804 2882 Size: 1149 773 6449 Fragments arranged by size: 6449 1149 773 BstYI r'GATC_y Cuts at: 1972 2155 4332 5701 6068 6453 6470 7922 8008 Size: 183 2177 1369 367 385 17 1452 86 Cuts at: 8008 8020 8366 1972 Size: 12 346 1977 Fragments arranged by size: 2177 1977 1452 1369 385 367 346 183 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2058 2844 2864 3147 3354 2058 Size: 786 20 283 207 7075 Fragments arranged by size: 7075 786 283 207 20 BtgI C'CryG_G Cuts at: 513 1065 3628 4365 4797 5598 513 Size: 552 2563 737 432 801 3286 Fragments arranged by size: 3286 2563 801 737 552 432 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7632 Fragments arranged by size: 7632 683 56 BtsI GCAGTG_nn' Cuts at: 2380 2656 5887 6681 6701 2380 Size: 276 3231 794 20 4050 Fragments arranged by size: 4050 3231 794 276 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8336 DraI TTT'AAA Cuts at: 5675 5675 Size: 8371 DraIII CAC_nnn'GTG Cuts at: 4609 4609 Size: 8371 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2077 7378 809 Size: 507 761 5301 1802 Fragments arranged by size: 5301 1802 761 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2224 5818 6707 Size: 54 1425 50 275 18 394 3594 889 Cuts at: 6707 8336 8 Size: 1629 43 Fragments arranged by size: 3594 1629 1425 889 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5818 1537 Size: 275 4006 4090 Fragments arranged by size: 4090 4006 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2708 3174 3799 5607 6301 1353 Size: 1355 466 625 1808 694 3423 Fragments arranged by size: 3423 1808 1355 694 625 466 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6958 7332 7478 387 Size: 1064 138 5369 374 146 1280 Fragments arranged by size: 5369 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2701 2701 Size: 8371 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2609 3354 3432 3486 4388 4590 4643 Size: 393 977 745 78 54 902 202 53 Cuts at: 4643 4890 5133 5283 5447 6434 7031 7818 8086 Size: 247 243 150 164 987 597 787 268 Cuts at: 8086 1239 Size: 1524 Fragments arranged by size: 1524 987 977 902 787 745 597 393 268 247 243 202 164 150 78 54 53 EcoO109I rG'GnC_Cy Cuts at: 3151 3151 Size: 8371 EcoRI G'AATT_C Cuts at: 4424 5680 6058 4424 Size: 1256 378 6737 Fragments arranged by size: 6737 1256 378 EcoRV GAT'ATC Cuts at: 2762 2762 Size: 8371 FspI TGC'GCA Cuts at: 6877 6877 Size: 8371 HaeII r_GCGC'y Cuts at: 1678 7518 1678 Size: 5840 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4228 4253 4260 4285 4712 Size: 32 135 32 2656 25 7 25 427 Cuts at: 4712 4744 5608 5640 7012 7044 7086 7118 1373 Size: 32 864 32 1372 32 42 32 2626 Fragments arranged by size: 2656 2626 1372 864 427 135 42 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 2530 5715 5961 669 Size: 988 281 592 3185 246 3079 Fragments arranged by size: 3185 3079 988 592 281 246 HindIII A'AGCT_T Cuts at: 748 2444 3774 748 Size: 1696 1330 5345 Fragments arranged by size: 5345 1696 1330 HpaI GTT'AAC Cuts at: 2530 5961 2530 Size: 3431 4940 KpnI G_GTAC'C Cuts at: 3587 5696 3587 Size: 2109 6262 MfeI C'AATT_G Cuts at: 5970 5970 Size: 8371 MluI A'CGCG_T Cuts at: 8247 8247 Size: 8371 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2397 4012 7485 7669 1305 Size: 1092 1615 3473 184 2007 Fragments arranged by size: 3473 2007 1615 1092 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6549 Fragments arranged by size: 6549 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2633 3638 4802 6329 6688 6847 518 Size: 648 1467 1005 1164 1527 359 159 2042 Fragments arranged by size: 2042 1527 1467 1164 1005 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2230 2248 3371 3508 5787 6464 7612 7857 Size: 624 18 1123 137 2279 677 1148 245 Cuts at: 7857 1606 Size: 2120 Fragments arranged by size: 2279 2120 1148 1123 677 624 245 137 18 NcoI C'CATG_G Cuts at: 513 1065 3628 4365 4797 513 Size: 552 2563 737 432 4087 Fragments arranged by size: 4087 2563 737 552 432 NdeI CA'TA_TG Cuts at: 387 3559 387 Size: 3172 5199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8371 NotI GC'GGCC_GC Cuts at: 5818 5818 Size: 8371 NspI r_CATG'y Cuts at: 1132 1229 2528 2642 2672 2714 4756 5729 7274 Size: 97 1299 114 30 42 2042 973 1545 Cuts at: 7274 1132 Size: 2229 Fragments arranged by size: 2229 2042 1545 1299 973 114 97 42 30 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8371 PciI A'CATG_T Cuts at: 4752 7270 4752 Size: 2518 5853 PflMI CCAn_nnn'nTGG Cuts at: 1108 3593 1108 Size: 2485 5886 PfoI T'CCnGG_A Cuts at: 2141 2451 3463 5260 2141 Size: 310 1012 1797 5252 Fragments arranged by size: 5252 1797 1012 310 PmeI GTTT'AAAC Cuts at: 5675 5675 Size: 8371 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6440 6472 7953 7985 6440 Size: 32 1481 32 6826 Fragments arranged by size: 6826 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3151 3151 Size: 8371 PshAI GACnn'nnGTC Cuts at: 1613 4360 1613 Size: 2747 5624 PsiI TTA'TAA Cuts at: 5941 5941 Size: 8371 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4944 4976 4944 Size: 32 8339 PstI C_TGCA'G Cuts at: 830 2992 4591 4903 5723 830 Size: 2162 1599 312 820 3478 Fragments arranged by size: 3478 2162 1599 820 312 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8371 PvuII CAG'CTG Cuts at: 2248 3371 3508 2248 Size: 1123 137 7111 Fragments arranged by size: 7111 1123 137 SacI G_AGCT'C Cuts at: 2703 2703 Size: 8371 SalI G'TCGA_C Cuts at: 1936 5713 1936 Size: 3777 4594 SapI GCTCTTCn'nnn_ Cuts at: 2708 2708 Size: 8371 SbfI CC_TGCA'GG Cuts at: 5723 5723 Size: 8371 ScaI AGT'ACT Cuts at: 3496 6619 8331 3496 Size: 3123 1712 3536 Fragments arranged by size: 3536 3123 1712 SfcI C'TryA_G Cuts at: 826 1045 2988 3322 4587 4777 4899 5719 6854 Size: 219 1943 334 1265 190 122 820 1135 Cuts at: 6854 7535 7726 826 Size: 681 191 1471 Fragments arranged by size: 1943 1471 1265 1135 820 681 334 219 191 190 122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8371 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8371 SmaI CCC'GGG Cuts at: 1872 5698 1872 Size: 3826 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2716 4376 6475 7376 7638 820 Size: 197 926 773 1660 2099 901 262 1553 Fragments arranged by size: 2099 1660 1553 926 901 773 262 197 SnaBI TAC'GTA Cuts at: 493 6162 493 Size: 5669 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8371 SphI G_CATG'C Cuts at: 1132 2672 2714 5729 1132 Size: 1540 42 3015 3774 Fragments arranged by size: 3774 3015 1540 42 SspI AAT'ATT Cuts at: 5 52 4162 6295 5 Size: 47 4110 2133 2081 Fragments arranged by size: 4110 2133 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8371 StyI C'CwwG_G Cuts at: 513 1065 2082 2856 3075 3628 4293 4365 4797 Size: 552 1017 774 219 553 665 72 432 Cuts at: 4797 5810 513 Size: 1013 3074 Fragments arranged by size: 3074 1017 1013 774 665 553 552 432 219 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2285 2347 2483 6398 6415 6568 6753 Size: 1255 705 62 136 3915 17 153 185 Cuts at: 6753 7198 325 Size: 445 1498 Fragments arranged by size: 3915 1498 1255 705 445 185 153 136 62 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3165 3494 4683 Size: 274 80 33 51 1443 1188 329 1189 Cuts at: 4683 4823 5387 6617 8329 96 Size: 140 564 1230 1712 138 Fragments arranged by size: 1712 1443 1230 1189 1188 564 329 274 140 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2147 2179 5583 5615 8331 8363 2147 Size: 32 3404 32 2716 32 2155 Fragments arranged by size: 3404 2716 2155 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2074 1316 Size: 758 7613 XbaI T'CTAG_A Cuts at: 5707 5707 Size: 8371 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8371 XmaI C'CCGG_G Cuts at: 1870 5696 1870 Size: 3826 4545 XmnI GAAnn'nnTTC Cuts at: 2137 3694 4515 5484 6500 2137 Size: 1557 821 969 1016 4008 Fragments arranged by size: 4008 1557 1016 969 821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6179 276 Size: 53 83 186 936 4645 2468 Fragments arranged by size: 4645 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI