(Linear) (Six-base) MAPSORT of: KIEE3501.seq Check: 9651 from: 1 to: 3666 KIEE3501.orf With 182 enzymes: SgfI * March 22, 2012 16:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2283 3666 Size: 2283 1383 Acc65I G'GTAC_C Cuts at: 0 1578 3666 Size: 1578 2088 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2383 2585 2638 2885 3128 3442 3666 Size: 2383 202 53 247 243 314 224 Fragments arranged by size: 2383 314 247 243 224 202 53 AflII C'TTAA_G Cuts at: 0 711 2371 3666 Size: 711 1660 1295 Fragments arranged by size: 1660 1295 711 AflIII A'CryG_T Cuts at: 0 2747 3666 Size: 2747 919 AleI CACnn'nnGTG Cuts at: 0 1633 3666 Size: 1633 2033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1008 1651 1979 3666 Size: 1008 643 328 1687 Fragments arranged by size: 1687 1008 643 328 AlwNI CAG_nnn'CTG Cuts at: 0 246 747 1366 3666 Size: 246 501 619 2300 Fragments arranged by size: 2300 619 501 246 ApaLI G'TGCA_C Cuts at: 0 993 3666 Size: 993 2673 ApoI r'AATT_y Cuts at: 0 1113 1909 2419 2546 3504 3588 3666 Size: 1113 796 510 127 958 84 78 Fragments arranged by size: 1113 958 796 510 127 84 78 AseI AT'TA_AT Cuts at: 0 2704 3666 Size: 2704 962 AvaI C'yCGr_G Cuts at: 0 310 674 3666 Size: 310 364 2992 Fragments arranged by size: 2992 364 310 BamHI G'GATC_C Cuts at: 0 150 3666 Size: 150 3516 BanI G'GyrC_C Cuts at: 0 324 1417 1578 1619 3365 3666 Size: 324 1093 161 41 1746 301 Fragments arranged by size: 1746 1093 324 301 161 41 BanII G_rGCy'C Cuts at: 0 681 698 977 3666 Size: 681 17 279 2689 Fragments arranged by size: 2689 681 279 17 BbsI GAAGACnn'nnnn_ Cuts at: 0 2369 3448 3666 Size: 2369 1079 218 Fragments arranged by size: 2369 1079 218 BbvCI CC'TCA_GC Cuts at: 0 1504 3666 Size: 1504 2162 BciVI GTATCCnnnnn_n' Cuts at: 0 1288 3666 Size: 1288 2378 Bme1580I G_kGCm'C Cuts at: 0 327 997 1422 3666 Size: 327 670 425 2244 Fragments arranged by size: 2244 670 425 327 BmrI ACTGGGnnnn_n' Cuts at: 0 496 1007 2237 3556 3666 Size: 496 511 1230 1319 110 Fragments arranged by size: 1319 1230 511 496 110 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 1349 1427 1481 3278 3666 Size: 604 745 78 54 1797 388 Fragments arranged by size: 1797 745 604 388 78 54 Bpu10I CC'TnA_GC Cuts at: 0 1504 3666 Size: 1504 2162 BsaI GGTCTCn'nnnn_ Cuts at: 0 113 3666 Size: 113 3553 BsaBI GATnn'nnATC Cuts at: 0 1304 2332 3666 Size: 1304 1028 1334 Fragments arranged by size: 1334 1304 1028 BsaHI Gr'CG_yC Cuts at: 0 82 3666 Size: 82 3584 BsaWI w'CCGG_w Cuts at: 0 122 187 553 1048 3666 Size: 122 65 366 495 2618 Fragments arranged by size: 2618 495 366 122 65 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2223 2253 2277 2307 2702 2732 3666 Size: 2223 30 24 30 395 30 934 Fragments arranged by size: 2223 934 395 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 35 271 1664 2128 3613 3666 Size: 35 236 1393 464 1485 53 Fragments arranged by size: 1485 1393 464 236 53 35 BseYI C'CCAG_C Cuts at: 0 246 3311 3666 Size: 246 3065 355 Fragments arranged by size: 3065 355 246 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2255 3666 Size: 2255 1411 BsiHKAI G_wGCw'C Cuts at: 0 698 997 2013 3666 Size: 698 299 1016 1653 Fragments arranged by size: 1653 1016 698 299 BsmI GAATG_Cn' Cuts at: 0 3087 3666 Size: 3087 579 BsmBI CGTCTCn'nnnn_ Cuts at: 0 3269 3666 Size: 3269 397 Bsp1286I G_dGCh'C Cuts at: 0 327 681 698 977 997 1422 2013 3666 Size: 327 354 17 279 20 425 591 1653 Fragments arranged by size: 1653 591 425 354 327 279 20 17 BspEI T'CCGG_A Cuts at: 0 553 3666 Size: 553 3113 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 299 2283 3666 Size: 299 1984 1383 Fragments arranged by size: 1984 1383 299 BsrBI CCG'CTC Cuts at: 0 551 3666 Size: 551 3115 BsrDI GCAATG_nn' Cuts at: 0 1883 3666 Size: 1883 1783 BssSI C'ACGA_G Cuts at: 0 229 3666 Size: 229 3437 BstAPI GCAn_nnn'nTGC Cuts at: 0 243 3559 3666 Size: 243 3316 107 Fragments arranged by size: 3316 243 107 BstBI TT'CG_AA Cuts at: 0 3246 3666 Size: 3246 420 BstEII G'GTnAC_C Cuts at: 0 261 490 2364 3109 3666 Size: 261 229 1874 745 557 Fragments arranged by size: 1874 745 557 261 229 BstXI CCAn_nnnn'nTGG Cuts at: 0 877 2026 2799 3666 Size: 877 1149 773 867 Fragments arranged by size: 1149 877 867 773 BstYI r'GATC_y Cuts at: 0 150 2327 3666 Size: 150 2177 1339 Fragments arranged by size: 2177 1339 150 Bsu36I CC'TnA_GG Cuts at: 0 53 839 859 1142 1349 3666 Size: 53 786 20 283 207 2317 Fragments arranged by size: 2317 786 283 207 53 20 BtgI C'CryG_G Cuts at: 0 1623 2360 2792 3593 3666 Size: 1623 737 432 801 73 Fragments arranged by size: 1623 801 737 432 73 BtsI GCAGTG_nn' Cuts at: 0 375 651 3666 Size: 375 276 3015 Fragments arranged by size: 3015 375 276 DraIII CAC_nnn'GTG Cuts at: 0 2604 3666 Size: 2604 1062 DrdI GACnn_nn'nnGTC Cuts at: 0 72 3666 Size: 72 3594 EaeI y'GGCC_r Cuts at: 0 219 3666 Size: 219 3447 EarI CTCTTCn'nnn_ Cuts at: 0 703 1169 1794 3602 3666 Size: 703 466 625 1808 64 Fragments arranged by size: 1808 703 625 466 64 EcoICRI GAG'CTC Cuts at: 0 696 3666 Size: 696 2970 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 1349 1427 1481 2383 2585 2638 2885 Size: 604 745 78 54 902 202 53 247 Cuts at: 2885 3128 3278 3442 3666 Size: 243 150 164 224 Fragments arranged by size: 902 745 604 247 243 224 202 164 150 78 54 53 EcoO109I rG'GnC_Cy Cuts at: 0 1146 3666 Size: 1146 2520 EcoRI G'AATT_C Cuts at: 0 2419 3666 Size: 2419 1247 EcoRV GAT'ATC Cuts at: 0 757 3666 Size: 757 2909 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2223 2248 2255 2280 2707 2739 3603 3635 Size: 2223 25 7 25 427 32 864 32 Cuts at: 3635 3666 Size: 31 Fragments arranged by size: 2223 864 427 32 32 31 25 25 7 HincII GTy'rAC Cuts at: 0 525 3666 Size: 525 3141 HindIII A'AGCT_T Cuts at: 0 439 1769 3666 Size: 439 1330 1897 Fragments arranged by size: 1897 1330 439 HpaI GTT'AAC Cuts at: 0 525 3666 Size: 525 3141 KpnI G_GTAC'C Cuts at: 0 1582 3666 Size: 1582 2084 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 392 2007 3666 Size: 392 1615 1659 Fragments arranged by size: 1659 1615 392 MslI CAynn'nnrTG Cuts at: 0 628 1633 2797 3666 Size: 628 1005 1164 869 Fragments arranged by size: 1164 1005 869 628 MspA1I CmG'CkG Cuts at: 0 225 243 1366 1503 3666 Size: 225 18 1123 137 2163 Fragments arranged by size: 2163 1123 225 137 18 NcoI C'CATG_G Cuts at: 0 1623 2360 2792 3666 Size: 1623 737 432 874 Fragments arranged by size: 1623 874 737 432 NdeI CA'TA_TG Cuts at: 0 1554 3666 Size: 1554 2112 NspI r_CATG'y Cuts at: 0 523 637 667 709 2751 3666 Size: 523 114 30 42 2042 915 Fragments arranged by size: 2042 915 523 114 42 30 PciI A'CATG_T Cuts at: 0 2747 3666 Size: 2747 919 PflMI CCAn_nnn'nTGG Cuts at: 0 1588 3666 Size: 1588 2078 PfoI T'CCnGG_A Cuts at: 0 136 446 1458 3255 3666 Size: 136 310 1012 1797 411 Fragments arranged by size: 1797 1012 411 310 136 PpuMI rG'GwC_Cy Cuts at: 0 1146 3666 Size: 1146 2520 PshAI GACnn'nnGTC Cuts at: 0 2355 3666 Size: 2355 1311 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 2939 2971 3666 Size: 2939 32 695 Fragments arranged by size: 2939 695 32 PstI C_TGCA'G Cuts at: 0 987 2586 2898 3666 Size: 987 1599 312 768 Fragments arranged by size: 1599 987 768 312 PvuII CAG'CTG Cuts at: 0 243 1366 1503 3666 Size: 243 1123 137 2163 Fragments arranged by size: 2163 1123 243 137 SacI G_AGCT'C Cuts at: 0 698 3666 Size: 698 2968 SapI GCTCTTCn'nnn_ Cuts at: 0 703 3666 Size: 703 2963 ScaI AGT'ACT Cuts at: 0 1491 3666 Size: 1491 2175 SfcI C'TryA_G Cuts at: 0 983 1317 2582 2772 2894 3666 Size: 983 334 1265 190 122 772 Fragments arranged by size: 1265 983 772 334 190 122 SmlI C'TyrA_G Cuts at: 0 711 2371 3666 Size: 711 1660 1295 Fragments arranged by size: 1660 1295 711 SphI G_CATG'C Cuts at: 0 667 709 3666 Size: 667 42 2957 Fragments arranged by size: 2957 667 42 SspI AAT'ATT Cuts at: 0 2157 3666 Size: 2157 1509 StyI C'CwwG_G Cuts at: 0 77 851 1070 1623 2288 2360 2792 3666 Size: 77 774 219 553 665 72 432 874 Fragments arranged by size: 874 774 665 553 432 219 77 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 280 342 478 3666 Size: 280 62 136 3188 Fragments arranged by size: 3188 280 136 62 TatI w'GTAC_w Cuts at: 0 1160 1489 2678 2818 3382 3666 Size: 1160 329 1189 140 564 284 Fragments arranged by size: 1189 1160 564 329 284 140 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 142 174 3578 3610 3666 Size: 142 32 3404 32 56 Fragments arranged by size: 3404 142 56 32 32 Tth111I GACn'n_nGTC Cuts at: 0 69 3666 Size: 69 3597 XmnI GAAnn'nnTTC Cuts at: 0 132 1689 2510 3479 3666 Size: 132 1557 821 969 187 Fragments arranged by size: 1557 969 821 187 132 Enzymes that do cut: AarI Acc65I AcuI AflII AflIII AleI AlfI AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HincII HindIII HpaI KpnI MmeI MslI MspA1I NcoI NdeI NspI PciI PflMI PfoI PpuMI PshAI PsrI PstI PvuII SacI SapI ScaI SfcI SmlI SphI SspI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AatII AccI AclI AfeI AgeI AhdI AloI ApaI AscI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsiEI BsiWI BspHI BsrFI BsrGI BssHII BstZ17I BtgZI ClaI CspCI DraI EagI EciI EcoNI FalI FseI FspI FspAI HaeII KasI MfeI MluI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI XbaI XcmI XhoI XmaI ZraI