################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaI BseAI BsgI BsiEI BsiHKAI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI BstXI BstYI CelII Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FseI HaeII HincII HindII HpaI KasI Ksp632I KspI MfeI MflI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 200 GGTACC G^GTACC AccI 1196 GTATAC GT^[AC][GT]AC AccII 1691 CGCG CG^CG AcyI 146 GACGCC G[GA]^CG[CT]C AocI 796 CCTGAGG CC^T[ACGT]AGG Asp718I 200 GGTACC G^GTACC AviII 1869 TGCGCA TGC^GCA BanI 200 GGTACC G^G[CT][GA]CC BclI 363 TGATCA T^GATCA BsaBI 943 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 146 GACGCC G[GA]^CG[CT]C BsiWI 1817 CGTACG C^GTACG BsmBI 144 GAGACG CGTCTC(1/5) BspMI 185 ACCTGC ACCTGC(4/8) Bst1107I 1196 GTATAC GTA^TAC BstAPI 1742 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1691 CGCG CG^CG BstZ17I 1196 GTATAC GTA^TAC Bsu36I 796 CCTGAGG CC^T[ACGT]AGG Cac8I 1784 GCGTGC GC[ACGT]^[ACGT]GC DraI 597 TTTAAA TTT^AAA Eco81I 796 CCTGAGG CC^T[ACGT]AGG FbaI 363 TGATCA T^GATCA FspI 1869 TGCGCA TGC^GCA HgaI 146 GACGC GACGC(5/10) Hin1I 146 GACGCC G[GA]^CG[CT]C KpnI 200 GGTACC GGTAC^C MamI 943 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1691 CGCG CG^CG NdeI 1680 CATATG CA^TATG PstI 44 CTGCAG CTGCA^G TaqI 2278 TCGA T^CGA TthHB8I 2278 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AvaII 29 GGTCC G^G[AT]CC 132 GGACC BbsI 919 GTCTTC GAAGAC(2/6) 1507 GTCTTC BcnI 33 CCCGG CC^[GC]GG 1049 CCGGG BpuAI 919 GTCTTC GAAGAC(2/6) 1507 GTCTTC BsaWI 1216 ACCGGA [AT]^CCGG[AT] 1468 ACCGGA BseRI 115 GAGGAG GAGGAG(10/8) 799 GAGGAG BsmAI 144 GAGAC GTCTC(1/5) 1728 GAGAC DsaI 25 CCATGG C^C[GA][CT]GG 253 CCATGG NciI 33 CCCGG CC^[GC]GG 1049 CCGGG NcoI 25 CCATGG C^CATGG 253 CCATGG PleI 16 GACTC GAGTC(4/5) 563 GACTC SfcI 44 CTGCAG C^T[GA][CT]AG 177 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI Asp700I BbvI BfaI BpmI BsaJI BsiYI BslI BsmFI BsmI Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NsiI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI