Restriction map for
pFN21AE3686
(Circular) (Six-base) MAPSORT of: pFN21AE3686.seq Check: 148 from: 1 to: 7042
pFN21AE3686 7042 bp
With 182 enzymes: SgfI *
March 28, 2012 12:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2199 2199
Size: 7042
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4852 278
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3202 4385 1937
Size: 1265 1183 4594
Fragments arranged by size:
4594 1265 1183
Acc65I G'GTAC_C
Cuts at: 2205 4363 2205
Size: 2158 4884
AclI AA'CG_TT
Cuts at: 1563 5169 5542 1563
Size: 3606 373 3063
Fragments arranged by size:
3606 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2064 2095 5105 6489 6757 1632
Size: 432 31 3010 1384 268 1917
Fragments arranged by size:
3010 1917 1384 432 268 31
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6845
AflIII A'CryG_T
Cuts at: 1203 5941 6918 1203
Size: 4738 977 1327
Fragments arranged by size:
4738 1327 977
AgeI A'CCGG_T
Cuts at: 5873 5873
Size: 7042
AhdI GACnn_n'nnGTC
Cuts at: 5771 5771
Size: 7042
AleI CACnn'nnGTG
Cuts at: 1166 3978 1166
Size: 2812 4230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3888 1184
Size: 2704 4338
AlwNI CAG_nnn'CTG
Cuts at: 2228 3417 3921 4785 6357 2228
Size: 1189 504 864 1572 2913
Fragments arranged by size:
2913 1572 1189 864 504
ApaLI G'TGCA_C
Cuts at: 5099 6255 5099
Size: 1156 5886
ApoI r'AATT_y
Cuts at: 1493 2211 2331 2343 2725 2793 2877 3045 3129
Size: 718 120 12 382 68 84 168 84
Cuts at: 3129 3381 3465 4351 4577 4729 6838 1493
Size: 252 84 886 226 152 2109 1697
Fragments arranged by size:
2109 1697 886 718 382 252 226 168
152 120 84 84 84 68 12
AseI AT'TA_AT
Cuts at: 160 1032 5596 160
Size: 872 4564 1606
Fragments arranged by size:
4564 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
AvaI C'yCGr_G
Cuts at: 1870 1943 4367 1870
Size: 73 2424 4545
Fragments arranged by size:
4545 2424 73
BamHI G'GATC_C
Cuts at: 4372 4739 4372
Size: 367 6675
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2205 4363 5818 618
Size: 325 217 627 418 2158 1455 1842
Fragments arranged by size:
2158 1842 1455 627 418 325 217
BanII G_rGCy'C
Cuts at: 1391 4361 1391
Size: 2970 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2917 3505 928
Size: 971 1018 588 4465
Fragments arranged by size:
4465 1018 971 588
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5233 5267 1903
Size: 34 3296 34 3678
Fragments arranged by size:
3678 3296 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4934 6144 4934
Size: 1210 5832
BclI T'GATC_A
Cuts at: 1548 2368 1548
Size: 820 6222
BfrBI ATG'CAT
Cuts at: 3289 4213 4309 3289
Size: 924 96 6022
Fragments arranged by size:
6022 924 96
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5653 136
Size: 107 122 71 631 605 3981 1525
Fragments arranged by size:
3981 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7037 7037
Size: 7042
BlpI GC'TnA_GC
Cuts at: 4459 4459
Size: 7042
Bme1580I G_kGCm'C
Cuts at: 1790 5103 6259 1790
Size: 3313 1156 2573
Fragments arranged by size:
3313 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2060 2098 1318
Size: 742 38 6262
Fragments arranged by size:
6262 742 38
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2170 3567 4773
Size: 416 53 83 399 24 753 1397 1206
Cuts at: 4773 5731 442
Size: 958 1753
Fragments arranged by size:
1753 1397 1206 958 753 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7042
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2358 2907 3411 3663 3915 4167 5702
Size: 1049 70 549 504 252 252 252 1535
Cuts at: 5702 1239
Size: 2579
Fragments arranged by size:
2579 1535 1049 549 504 252 252 252
70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5167 6032 6330 5167
Size: 865 298 5879
Fragments arranged by size:
5879 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5705 882
Size: 617 4206 2219
Fragments arranged by size:
4206 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4833 493
Size: 711 3629 2702
Fragments arranged by size:
3629 2702 711
BsaBI GATnn'nnATC
Cuts at: 2952 7036 2952
Size: 4084 2958
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2152 4849 5231 5866
Size: 53 83 186 936 619 2697 382 635
Cuts at: 5866 275
Size: 1451
Fragments arranged by size:
2697 1451 936 635 619 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3221 3473 5475 5873 6147 6294 6705 3221
Size: 252 2002 398 274 147 411 3558
Fragments arranged by size:
3558 2002 411 398 274 252 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6817
Fragments arranged by size:
6817 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2819 1186
Size: 949 684 5409
Fragments arranged by size:
5409 949 684
BseYI C'CCAG_C
Cuts at: 6245 6741 6245
Size: 496 6546
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6469
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4492 5253 6281 1540
Size: 275 186 2491 761 1028 2301
Fragments arranged by size:
2491 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5103 5188 6259 5103
Size: 85 1071 5886
Fragments arranged by size:
5886 1071 85
BsiWI C'GTAC_G
Cuts at: 3822 3822
Size: 7042
BsmI GAATG_Cn'
Cuts at: 2713 3161 3245 3413 3749 3842 3917 4553 4646
Size: 448 84 168 336 93 75 636 93
Cuts at: 4646 2713
Size: 5109
Fragments arranged by size:
5109 636 448 336 168 93 93 84
75
BsmBI CGTCTCn'nnnn_
Cuts at: 2143 2143
Size: 7042
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4361 5103 5188 6259 1391
Size: 399 2571 742 85 1071 2174
Fragments arranged by size:
2571 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6705 6705
Size: 7042
BspHI T'CATG_A
Cuts at: 4931 6661 4931
Size: 1730 5312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 4397 844
Size: 1355 2198 3489
Fragments arranged by size:
3489 2198 1355
BsrBI CCG'CTC
Cuts at: 1394 1823 4929 1394
Size: 429 3106 3507
Fragments arranged by size:
3507 3106 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3250 3670 3870 5537 5711 65
Size: 1183 2002 420 200 1667 174 1396
Fragments arranged by size:
2002 1667 1396 1183 420 200 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5686 5873 1700
Size: 110 3876 187 2869
Fragments arranged by size:
3876 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2950 3494 4250 96
Size: 2854 544 756 2888
Fragments arranged by size:
2888 2854 756 544
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7042
BssSI C'ACGA_G
Cuts at: 5102 6114 5102
Size: 1012 6030
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3753 794
Size: 2959 4083
BstBI TT'CG_AA
Cuts at: 5846 5846
Size: 7042
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7042
BstYI r'GATC_y
Cuts at: 1972 4372 4739 5124 5141 6593 6679 6691 7037
Size: 2400 367 385 17 1452 86 12 346
Cuts at: 7037 1972
Size: 1977
Fragments arranged by size:
2400 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3203 3203
Size: 7042
Bsu36I CC'TnA_GG
Cuts at: 2802 2802
Size: 7042
BtgI C'CryG_G
Cuts at: 513 1065 2004 2030 2258 513
Size: 552 939 26 228 5297
Fragments arranged by size:
5297 939 552 228 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6303
Fragments arranged by size:
6303 683 56
BtsI GCAGTG_nn'
Cuts at: 3082 3166 3418 4558 5352 5372 3082
Size: 84 252 1140 794 20 4752
Fragments arranged by size:
4752 1140 794 252 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7042
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3232 3267 3652 3687 529
Size: 35 2668 35 385 35 3884
Fragments arranged by size:
3884 2668 385 35 35 35
DraI TTT'AAA
Cuts at: 2604 4346 2604
Size: 1742 5300
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6049 809
Size: 507 4733 1802
Fragments arranged by size:
4733 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4489 5378 7007
Size: 54 1425 50 275 18 2659 889 1629
Cuts at: 7007 8
Size: 43
Fragments arranged by size:
2659 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4489 1537
Size: 275 2677 4090
Fragments arranged by size:
4090 2677 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4972 1353
Size: 3619 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5629 6003 6149 387
Size: 1064 138 4040 374 146 1280
Fragments arranged by size:
4040 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2064 2095 2288 2358 2907 3411 3663
Size: 393 432 31 193 70 549 504 252
Cuts at: 3663 3915 4167 5105 5702 6489 6757 1239
Size: 252 252 938 597 787 268 1524
Fragments arranged by size:
1524 938 787 597 549 504 432 393
268 252 252 252 193 70 31
EcoRI G'AATT_C
Cuts at: 2331 2877 3129 3381 3465 4351 4729 2331
Size: 546 252 252 84 886 378 4644
Fragments arranged by size:
4644 886 546 378 252 252 84
FspI TGC'GCA
Cuts at: 3876 5548 3876
Size: 1672 5370
HaeII r_GCGC'y
Cuts at: 1678 6189 1678
Size: 4511 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2353 2385 3096 3128 4020
Size: 32 135 32 781 32 711 32 892
Cuts at: 4020 4052 4272 4304 5683 5715 5757 5789 1373
Size: 32 220 32 1379 32 42 32 2626
Fragments arranged by size:
2626 1379 892 781 711 220 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4386 4632 669
Size: 988 281 2448 246 3079
Fragments arranged by size:
3079 2448 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2546 2810 4182 748
Size: 1798 264 1372 3608
Fragments arranged by size:
3608 1798 1372 264
HpaI GTT'AAC
Cuts at: 4632 4632
Size: 7042
KpnI G_GTAC'C
Cuts at: 2209 4367 2209
Size: 2158 4884
MfeI C'AATT_G
Cuts at: 4641 4641
Size: 7042
MluI A'CGCG_T
Cuts at: 6918 6918
Size: 7042
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 6156 6340 1305
Size: 915 3936 184 2007
Fragments arranged by size:
3936 2007 915 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5220
Fragments arranged by size:
5220 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2577 2958 3978 5000 5359 5518 518
Size: 648 1411 381 1020 1022 359 159 2042
Fragments arranged by size:
2042 1411 1022 1020 648 381 359 159
MspA1I CmG'CkG
Cuts at: 1606 4458 5135 6283 6528 1606
Size: 2852 677 1148 245 2120
Fragments arranged by size:
2852 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2030 2258 513
Size: 552 939 26 228 5297
Fragments arranged by size:
5297 939 552 228 26
NdeI CA'TA_TG
Cuts at: 387 3686 387
Size: 3299 3743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7042
NotI GC'GGCC_GC
Cuts at: 4489 4489
Size: 7042
NsiI A_TGCA'T
Cuts at: 3291 4215 4311 3291
Size: 924 96 6022
Fragments arranged by size:
6022 924 96
NspI r_CATG'y
Cuts at: 1132 1229 4400 5945 1132
Size: 97 3171 1545 2229
Fragments arranged by size:
3171 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7042
PciI A'CATG_T
Cuts at: 5941 5941
Size: 7042
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7042
PfoI T'CCnGG_A
Cuts at: 2335 2335
Size: 7042
PmeI GTTT'AAAC
Cuts at: 4346 4346
Size: 7042
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5111 5143 6624 6656 5111
Size: 32 1481 32 5497
Fragments arranged by size:
5497 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7042
PsiI TTA'TAA
Cuts at: 4612 4612
Size: 7042
PstI C_TGCA'G
Cuts at: 830 2053 4394 830
Size: 1223 2341 3478
Fragments arranged by size:
3478 2341 1223
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7042
SalI G'TCGA_C
Cuts at: 1936 4384 1936
Size: 2448 4594
SbfI CC_TGCA'GG
Cuts at: 4394 4394
Size: 7042
ScaI AGT'ACT
Cuts at: 5290 7002 5290
Size: 1712 5330
SfcI C'TryA_G
Cuts at: 826 1045 2049 2182 4390 5525 6206 6397 826
Size: 219 1004 133 2208 1135 681 191 1471
Fragments arranged by size:
2208 1471 1135 1004 681 219 191 133
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7042
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7042
SmaI CCC'GGG
Cuts at: 1872 4369 1872
Size: 2497 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5146 6047 6309 820
Size: 197 926 3203 901 262 1553
Fragments arranged by size:
3203 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4833 493
Size: 4340 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7042
SphI G_CATG'C
Cuts at: 1132 4400 1132
Size: 3268 3774
SspI AAT'ATT
Cuts at: 5 52 4966 5
Size: 47 4914 2081
Fragments arranged by size:
4914 2081 47
StuI AGG'CCT
Cuts at: 1517 3344 3848 4016 4268 1517
Size: 1827 504 168 252 4291
Fragments arranged by size:
4291 1827 504 252 168
StyI C'CwwG_G
Cuts at: 513 1065 2004 2030 2258 2644 4481 513
Size: 552 939 26 228 386 1837 3074
Fragments arranged by size:
3074 1837 939 552 386 228 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 4041 5069 5086 5239 5424 5869
Size: 1255 1215 1246 1028 17 153 185 445
Cuts at: 5869 325
Size: 1498
Fragments arranged by size:
1498 1255 1246 1215 1028 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2157 2822 2950
Size: 274 80 33 51 1443 180 665 128
Cuts at: 2950 2990 3494 4250 5288 7000 96
Size: 40 504 756 1038 1712 138
Fragments arranged by size:
1712 1443 1038 756 665 504 274 180
138 128 80 51 40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7002 7034 7002
Size: 32 7010
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7042
XbaI T'CTAG_A
Cuts at: 4378 4378
Size: 7042
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7042
XmaI C'CCGG_G
Cuts at: 1870 4367 1870
Size: 2497 4545
XmnI GAAnn'nnTTC
Cuts at: 3260 3344 3428 3764 3932 4100 4184 5171 3260
Size: 84 84 336 168 168 84 987 5131
Fragments arranged by size:
5131 987 336 168 168 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4850 276
Size: 53 83 186 936 3316 2468
Fragments arranged by size:
3316 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BstXI DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI