Restriction map for
pFN21AE3686
(Linear) (Six-base) MAPSORT of: KIEE3686.seq Check: 705 from: 1 to: 2337
KIEE3686.orf
With 182 enzymes: SgfI *
March 22, 2012 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 194 2337
Size: 194 2143
AccI GT'mk_AC
Cuts at: 0 1197 2337
Size: 1197 1140
Acc65I G'GTAC_C
Cuts at: 0 200 2337
Size: 200 2137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 90 2337
Size: 59 31 2247
Fragments arranged by size:
2247 59 31
AleI CACnn'nnGTG
Cuts at: 0 1973 2337
Size: 1973 364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1883 2337
Size: 1883 454
AlwNI CAG_nnn'CTG
Cuts at: 0 223 1412 1916 2337
Size: 223 1189 504 421
Fragments arranged by size:
1189 504 421 223
ApoI r'AATT_y
Cuts at: 0 206 326 338 720 788 872 1040 1124
Size: 206 120 12 382 68 84 168 84
Cuts at: 1124 1376 1460 2337
Size: 252 84 877
Fragments arranged by size:
877 382 252 206 168 120 84 84
84 68 12
BanI G'GyrC_C
Cuts at: 0 200 2337
Size: 200 2137
BbsI GAAGACnn'nnnn_
Cuts at: 0 912 1500 2337
Size: 912 588 837
Fragments arranged by size:
912 837 588
BclI T'GATC_A
Cuts at: 0 363 2337
Size: 363 1974
BfrBI ATG'CAT
Cuts at: 0 1284 2208 2304 2337
Size: 1284 924 96 33
Fragments arranged by size:
1284 924 96 33
BmgBI CAC'GTC
Cuts at: 0 55 93 2337
Size: 55 38 2244
Fragments arranged by size:
2244 55 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 1562 2337
Size: 165 1397 775
Fragments arranged by size:
1397 775 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 353 902 1406 1658 1910 2162 2337
Size: 283 70 549 504 252 252 252 175
Fragments arranged by size:
549 504 283 252 252 252 175 70
BsaBI GATnn'nnATC
Cuts at: 0 947 2337
Size: 947 1390
BsaHI Gr'CG_yC
Cuts at: 0 147 2337
Size: 147 2190
BsaWI w'CCGG_w
Cuts at: 0 1216 1468 2337
Size: 1216 252 869
Fragments arranged by size:
1216 869 252
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 814 2337
Size: 130 684 1523
Fragments arranged by size:
1523 684 130
BsiWI C'GTAC_G
Cuts at: 0 1817 2337
Size: 1817 520
BsmI GAATG_Cn'
Cuts at: 0 708 1156 1240 1408 1744 1837 1912 2337
Size: 708 448 84 168 336 93 75 425
Fragments arranged by size:
708 448 425 336 168 93 84 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 138 2337
Size: 138 2199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 2337
Size: 194 2143
BsrDI GCAATG_nn'
Cuts at: 0 1245 1665 1865 2337
Size: 1245 420 200 472
Fragments arranged by size:
1245 472 420 200
BsrGI T'GTAC_A
Cuts at: 0 945 1489 2245 2337
Size: 945 544 756 92
Fragments arranged by size:
945 756 544 92
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1748 2337
Size: 1748 589
BstZ17I GTA'TAC
Cuts at: 0 1198 2337
Size: 1198 1139
Bsu36I CC'TnA_GG
Cuts at: 0 797 2337
Size: 797 1540
BtgI C'CryG_G
Cuts at: 0 25 253 2337
Size: 25 228 2084
Fragments arranged by size:
2084 228 25
BtsI GCAGTG_nn'
Cuts at: 0 1077 1161 1413 2337
Size: 1077 84 252 924
Fragments arranged by size:
1077 924 252 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1227 1262 1647 1682 2337
Size: 1227 35 385 35 655
Fragments arranged by size:
1227 655 385 35 35
DraI TTT'AAA
Cuts at: 0 599 2337
Size: 599 1738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 90 283 353 902 1406 1658 1910
Size: 59 31 193 70 549 504 252 252
Cuts at: 1910 2162 2337
Size: 252 175
Fragments arranged by size:
549 504 252 252 252 193 175 70
59 31
EcoRI G'AATT_C
Cuts at: 0 326 872 1124 1376 1460 2337
Size: 326 546 252 252 84 877
Fragments arranged by size:
877 546 326 252 252 84
FspI TGC'GCA
Cuts at: 0 1871 2337
Size: 1871 466
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 348 380 1091 1123 2015 2047 2267 2299
Size: 348 32 711 32 892 32 220 32
Cuts at: 2299 2337
Size: 38
Fragments arranged by size:
892 711 348 220 38 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 541 805 2177 2337
Size: 541 264 1372 160
Fragments arranged by size:
1372 541 264 160
KpnI G_GTAC'C
Cuts at: 0 204 2337
Size: 204 2133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 2337
Size: 215 2122
MslI CAynn'nnrTG
Cuts at: 0 572 953 1973 2337
Size: 572 381 1020 364
Fragments arranged by size:
1020 572 381 364
NcoI C'CATG_G
Cuts at: 0 25 253 2337
Size: 25 228 2084
Fragments arranged by size:
2084 228 25
NdeI CA'TA_TG
Cuts at: 0 1681 2337
Size: 1681 656
NsiI A_TGCA'T
Cuts at: 0 1286 2210 2306 2337
Size: 1286 924 96 31
Fragments arranged by size:
1286 924 96 31
PfoI T'CCnGG_A
Cuts at: 0 330 2337
Size: 330 2007
PstI C_TGCA'G
Cuts at: 0 48 2337
Size: 48 2289
SfcI C'TryA_G
Cuts at: 0 44 177 2337
Size: 44 133 2160
Fragments arranged by size:
2160 133 44
StuI AGG'CCT
Cuts at: 0 1339 1843 2011 2263 2337
Size: 1339 504 168 252 74
Fragments arranged by size:
1339 504 252 168 74
StyI C'CwwG_G
Cuts at: 0 25 253 639 2337
Size: 25 228 386 1698
Fragments arranged by size:
1698 386 228 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 2036 2337
Size: 790 1246 301
Fragments arranged by size:
1246 790 301
TatI w'GTAC_w
Cuts at: 0 152 817 945 985 1489 2245 2337
Size: 152 665 128 40 504 756 92
Fragments arranged by size:
756 665 504 152 128 92 40
XmnI GAAnn'nnTTC
Cuts at: 0 1255 1339 1423 1759 1927 2095 2179 2337
Size: 1255 84 84 336 168 168 84 158
Fragments arranged by size:
1255 336 168 168 158 84 84 84
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlfI AlwNI ApoI
BanI BbsI BclI BfrBI BmgBI BmrI BpmI BsaBI
BsaHI BsaWI BseRI BsiWI BsmI BsmBI BspMI BsrDI
BsrGI BstAPI BstZ17I Bsu36I BtgI BtsI CspCI DraI
Eco57MI EcoRI FspI Hin4I HindIII KpnI MmeI MslI
NcoI NdeI NsiI PfoI PstI SfcI StuI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstYI
BtgZI ClaI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI HaeII
HincII HpaI KasI MfeI MluI MscI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI