################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BalI BbrPI BbsI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfaNI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 1397 GGTACC G^GTACC AcsI 933 GAATTT [GA]^AATT[CT] AgeI 1394 ACCGGT A^CCGGT AlwNI 1042 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 467 CCTCAGG CC^T[ACGT]AGG ApoI 933 GAATTT [GA]^AATT[CT] Asp718I 1397 GGTACC G^GTACC BamHI 166 GGATCC G^GATCC BbeI 86 GGCGCC GGCGC^C BbvCI 948 GCTGAGG CCTCAGC(-5/-2) BcgI 368 CGAAACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 801 GTATCC GTATCC(6/5) BglI 1411 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 341 GAGACC GGTCTC(1/5) BsaWI 1394 ACCGGT [AT]^CCGG[AT] BsiEI 1359 CGGCCG CG[GA][CT]^CG BsmBI 686 CGTCTC CGTCTC(1/5) BstEII 1287 GGTCACC G^GT[ACGT]ACC BstPI 1287 GGTCACC G^GT[ACGT]ACC BstYI 166 GGATCC [GA]^GATC[CT] Bsu36I 467 CCTCAGG CC^T[ACGT]AGG DraIII 1004 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1359 CGGCCG [CT]^GGCC[GA] EagI 1359 CGGCCG C^GGCCG EclXI 1359 CGGCCG C^GGCCG Eco52I 1359 CGGCCG C^GGCCG Eco81I 467 CCTCAGG CC^T[ACGT]AGG EcoO65I 1287 GGTCACC G^GT[ACGT]ACC HaeII 86 GGCGCC [GA]GCGC^[CT] HinfI 148 GAATC G^A[ACGT]TC KasI 86 GGCGCC G^GCGCC KpnI 1397 GGTACC GGTAC^C KspI 82 CCGCGG CCGC^GG MaeII 1073 ACGT A^CGT MflI 166 GGATCC [GA]^GATC[CT] NaeI 761 GCCGGC GCC^GGC NarI 86 GGCGCC GG^CGCC NgoMIV 761 GCCGGC G^CCGGC NspI 797 GCATGT [GA]CATG^[CT] PflMI 1003 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1394 ACCGGT A^CCGGT PvuII 1052 CAGCTG CAG^CTG SacI 291 GAGCTC GAGCT^C SacII 82 CCGCGG CCGC^GG SbfI 1413 CCTGCAGG CCTGCA^GG SfoI 86 GGCGCC GGC^GCC Sse8387I 1413 CCTGCAGG CCTGCA^GG TfiI 148 GAATC G^A[AT]TC Van91I 1003 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 166 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 86 GGCGCC G[GA]^CG[CT]C 1375 GACGCC AfaI 1014 GTAC GT^AC 1398 GTAC ApaI 330 GGGCCC GGGCC^C 680 GGGCCC Asp700I 837 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 969 GAACCGCTTC AspHI 291 GAGCTC G[AT]GC[AT]^C 724 GTGCTC BanI 86 GGCGCC G^G[CT][GA]CC 1397 GGTACC BfaI 1166 CTAG C^TAG 1387 CTAG BsaHI 86 GGCGCC G[GA]^CG[CT]C 1375 GACGCC BsgI 817 CTGCAC GTGCAG(16/14) 1222 CTGCAC BsiHKAI 291 GAGCTC G[AT]GC[AT]^C 724 GTGCTC BsmAI 341 GAGAC GTCTC(1/5) 687 GTCTC BsrFI 761 GCCGGC [GA]^CCGG[CT] 1394 ACCGGT BsrI 482 CCAGT ACTGG(1/-1) 1439 CCAGT BstF5I 1149 CATCC GGATG(2/0) 12 GGATG Cfr10I 761 GCCGGC [GA]^CCGG[CT] 1394 ACCGGT EcoNI 21 CCTCCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1035 CCTTCGGCAGG FokI 1149 CATCC GGATG(9/13) 12 GGATG HgaI 1200 GACGC GACGC(5/10) 1375 GACGC Hin1I 86 GGCGCC G[GA]^CG[CT]C 1375 GACGCC MaeI 1166 CTAG C^TAG 1387 CTAG MaeIII 1169 GTGAC ^GT[ACGT]AC 1288 GTCAC MslI 1020 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1324 CACGCTGATG PpuMI 461 AGGACCC [GA]G^G[AT]CC[CT] 1142 GGGTCCC PstI 1351 CTGCAG CTGCA^G 1414 CTGCAG RsaI 1014 GTAC GT^AC 1398 GTAC SapI 288 GAAGAGC GCTCTTC(1/4) 492 GAAGAGC SfcI 1351 CTGCAG C^T[GA][CT]AG 1414 CTGCAG SmaI 713 CCCGGG CCC^GGG 770 CCCGGG StuI 1295 AGGCCT AGG^CCT 1418 AGGCCT Tsp45I 1169 GTGAC ^GT[GC]AC 1288 GTCAC Tsp509I 886 AATT ^AATT 934 AATT XmaCI 713 CCCGGG C^CCGGG 770 CCCGGG XmaI 713 CCCGGG C^CCGGG 770 CCCGGG XmnI 837 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 969 GAACCGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AccII AciI AluI AlwI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BssKI BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI StyI TaqI TseI TspRI TthHB8I