(Circular) (Six-base) MAPSORT of: pFN21AE3684.seq Check: 8422 from: 1 to: 6172 pFN21AE3684 6172 bp With 182 enzymes: SgfI * January 27, 2015 16:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3982 278 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3515 1937 Size: 1578 4594 Acc65I G'GTAC_C Cuts at: 3402 3493 3402 Size: 91 6081 AclI AA'CG_TT Cuts at: 1563 4299 4672 1563 Size: 2736 373 3063 Fragments arranged by size: 3063 2736 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 2573 4235 5619 5887 1632 Size: 698 243 1662 1384 268 1917 Fragments arranged by size: 1917 1662 1384 698 268 243 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 5071 6048 1203 Size: 3868 977 1327 Fragments arranged by size: 3868 1327 977 AgeI A'CCGG_T Cuts at: 3399 5003 3399 Size: 1604 4568 AhdI GACnn_n'nnGTC Cuts at: 4901 4901 Size: 6172 AleI CACnn'nnGTG Cuts at: 1166 3029 1166 Size: 1863 4309 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6172 AlwNI CAG_nnn'CTG Cuts at: 3052 3915 5487 3052 Size: 863 1572 3737 Fragments arranged by size: 3737 1572 863 ApaI G_GGCC'C Cuts at: 2339 2689 2339 Size: 350 5822 ApaLI G'TGCA_C Cuts at: 4229 5385 4229 Size: 1156 5016 ApoI r'AATT_y Cuts at: 1493 2938 3481 3707 3859 5968 1493 Size: 1445 543 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 543 226 152 AseI AT'TA_AT Cuts at: 160 1032 4726 160 Size: 872 3694 1606 Fragments arranged by size: 3694 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 2718 2775 2925 3497 1870 Size: 73 775 57 150 572 4545 Fragments arranged by size: 4545 775 572 150 73 57 BamHI G'GATC_C Cuts at: 2171 3502 3869 2171 Size: 1331 367 4474 Fragments arranged by size: 4474 1331 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 3402 3493 4948 618 Size: 325 217 627 304 1311 91 1455 1842 Fragments arranged by size: 1842 1455 1311 627 325 304 217 91 BanII G_rGCy'C Cuts at: 1391 2078 2300 2339 2483 2504 2689 3491 1391 Size: 687 222 39 144 21 185 802 4072 Fragments arranged by size: 4072 802 687 222 185 144 39 21 BbeI G_GCGC'C Cuts at: 2095 2095 Size: 6172 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5201 BbvCI CC'TCA_GC Cuts at: 2954 2954 Size: 6172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2362 2396 4363 4397 1903 Size: 34 50 34 341 34 1967 34 3678 Fragments arranged by size: 3678 1967 341 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2817 4064 5274 2817 Size: 1247 1210 3715 Fragments arranged by size: 3715 1247 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3422 4783 136 Size: 107 122 71 631 605 1750 1361 1525 Fragments arranged by size: 1750 1525 1361 631 605 122 107 71 BglII A'GATC_T Cuts at: 6167 6167 Size: 6172 BlpI GC'TnA_GC Cuts at: 3589 3589 Size: 6172 Bme1580I G_kGCm'C Cuts at: 1790 2339 2689 4233 5389 1790 Size: 549 350 1544 1156 2573 Fragments arranged by size: 2573 1544 1156 549 350 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6172 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3903 4861 442 Size: 416 53 83 399 24 2486 958 1753 Fragments arranged by size: 2486 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6172 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2314 2314 Size: 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2388 3230 3427 4832 1239 Size: 1149 842 197 1405 2579 Fragments arranged by size: 2579 1405 1149 842 197 Bpu10I CC'TnA_GC Cuts at: 2072 2126 2954 2072 Size: 54 828 5290 Fragments arranged by size: 5290 828 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4297 5162 5460 4297 Size: 865 298 5009 Fragments arranged by size: 5009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2340 4835 882 Size: 617 841 2495 2219 Fragments arranged by size: 2495 2219 841 617 BsaAI yAC'GTr Cuts at: 493 1204 3963 493 Size: 711 2759 2702 Fragments arranged by size: 2759 2702 711 BsaBI GATnn'nnATC Cuts at: 6166 6166 Size: 6172 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2092 3381 3979 4361 Size: 53 83 186 936 559 1289 598 382 Cuts at: 4361 4996 275 Size: 635 1451 Fragments arranged by size: 1451 1289 936 635 598 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3399 4605 5003 5277 5424 5835 3399 Size: 1206 398 274 147 411 3736 Fragments arranged by size: 3736 1206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2885 2915 3248 3278 1178 Size: 30 165 30 1482 30 333 30 4072 Fragments arranged by size: 4072 1482 333 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2213 2216 2225 2234 2249 2332 2612 Size: 1024 3 3 9 9 15 83 280 Cuts at: 2612 2615 2618 2693 2943 1186 Size: 3 3 75 250 4415 Fragments arranged by size: 4415 1024 280 250 83 75 15 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2482 2503 3082 5375 5871 2482 Size: 21 579 2293 496 2783 Fragments arranged by size: 2783 2293 579 496 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2807 3212 1163 Size: 573 1071 405 4123 Fragments arranged by size: 4123 1071 573 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 3622 4383 5411 1540 Size: 275 186 1366 255 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 255 186 BsiHKAI G_wGCw'C Cuts at: 2300 2733 4233 4318 5389 2300 Size: 433 1500 85 1071 3083 Fragments arranged by size: 3083 1500 1071 433 85 BsmI GAATG_Cn' Cuts at: 3683 3776 3683 Size: 93 6079 BsmBI CGTCTCn'nnnn_ Cuts at: 2697 2697 Size: 6172 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2300 2339 2483 2504 2689 2733 Size: 399 288 222 39 144 21 185 44 Cuts at: 2733 3491 4233 4318 5389 1391 Size: 758 742 85 1071 2174 Fragments arranged by size: 2174 1071 758 742 399 288 222 185 144 85 44 39 21 BspEI T'CCGG_A Cuts at: 5835 5835 Size: 6172 BspHI T'CATG_A Cuts at: 4061 5791 4061 Size: 1730 4442 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3527 844 Size: 2683 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4059 1394 Size: 429 2236 3507 Fragments arranged by size: 3507 2236 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4667 4841 65 Size: 1183 3419 174 1396 Fragments arranged by size: 3419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2766 3399 4816 5003 1700 Size: 110 956 633 1417 187 2869 Fragments arranged by size: 2869 1417 956 633 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6172 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6172 BssSI C'ACGA_G Cuts at: 4232 5244 4232 Size: 1012 5160 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6172 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 3292 1184 Size: 2108 4064 BstXI CCAn_nnnn'nTGG Cuts at: 2518 2968 3082 3246 2518 Size: 450 114 164 5444 Fragments arranged by size: 5444 450 164 114 BstYI r'GATC_y Cuts at: 1972 2171 3502 3869 4254 4271 5723 5809 5821 Size: 199 1331 367 385 17 1452 86 12 Cuts at: 5821 6167 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1331 385 367 346 199 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2473 2473 Size: 6172 BtgI C'CryG_G Cuts at: 513 1065 2087 2166 2700 3091 513 Size: 552 1022 79 534 391 3594 Fragments arranged by size: 3594 1022 552 534 391 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3418 487 Size: 683 56 2192 3241 Fragments arranged by size: 3241 2192 683 56 BtsI GCAGTG_nn' Cuts at: 3688 4482 4502 3688 Size: 794 20 5358 Fragments arranged by size: 5358 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6137 DraI TTT'AAA Cuts at: 3476 3476 Size: 6172 DraIII CAC_nnn'GTG Cuts at: 3014 3014 Size: 6172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5179 809 Size: 507 3863 1802 Fragments arranged by size: 3863 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3364 3619 4508 Size: 54 1425 50 275 18 1534 255 889 Cuts at: 4508 6137 8 Size: 1629 43 Fragments arranged by size: 1629 1534 1425 889 275 255 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 3619 1537 Size: 275 1552 255 4090 Fragments arranged by size: 4090 1552 275 255 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 2202 2226 2288 2492 2589 4102 1353 Size: 776 73 24 62 204 97 1513 3423 Fragments arranged by size: 3423 1513 776 204 97 73 62 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3389 4759 5133 5279 387 Size: 1064 138 1800 1370 374 146 1280 Fragments arranged by size: 1800 1370 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2298 2298 Size: 6172 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 2388 2573 3230 3427 4235 4832 Size: 393 698 58 185 657 197 808 597 Cuts at: 4832 5619 5887 1239 Size: 787 268 1524 Fragments arranged by size: 1524 808 787 698 657 597 393 268 197 185 58 EcoNI CCTnn'n_nnAGG Cuts at: 2030 3044 2030 Size: 1014 5158 EcoO109I rG'GnC_Cy Cuts at: 2083 2146 2335 2467 2685 2686 3148 2083 Size: 63 189 132 218 1 462 5107 Fragments arranged by size: 5107 462 218 189 132 63 1 EcoRI G'AATT_C Cuts at: 3481 3859 3481 Size: 378 5794 FspI TGC'GCA Cuts at: 4678 4678 Size: 6172 HaeII r_GCGC'y Cuts at: 1678 2095 5319 1678 Size: 417 3224 2531 Fragments arranged by size: 3224 2531 417 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3248 3280 4813 4845 4887 Size: 32 135 32 1676 32 1533 32 42 Cuts at: 4887 4919 1373 Size: 32 2626 Fragments arranged by size: 2626 1676 1533 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3516 3762 669 Size: 988 281 1578 246 3079 Fragments arranged by size: 3079 1578 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6172 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KasI G'GCGC_C Cuts at: 2091 2091 Size: 6172 KpnI G_GTAC'C Cuts at: 3406 3497 3406 Size: 91 6081 MfeI C'AATT_G Cuts at: 3771 3771 Size: 6172 MluI A'CGCG_T Cuts at: 6048 6048 Size: 6172 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2985 5286 5470 1305 Size: 1680 2301 184 2007 Fragments arranged by size: 2301 2007 1680 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4350 Fragments arranged by size: 4350 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3029 3333 4130 4489 4648 518 Size: 648 1863 304 797 359 159 2042 Fragments arranged by size: 2042 1863 797 648 359 304 159 MspA1I CmG'CkG Cuts at: 1606 2089 3059 3409 3588 4265 5413 5658 1606 Size: 483 970 350 179 677 1148 245 2120 Fragments arranged by size: 2120 1148 970 677 483 350 245 179 NaeI GCC'GGC Cuts at: 2768 2768 Size: 6172 NarI GG'CG_CC Cuts at: 2092 2092 Size: 6172 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5620 NdeI CA'TA_TG Cuts at: 387 387 Size: 6172 NgoMIV G'CCGG_C Cuts at: 2766 2766 Size: 6172 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6172 NotI GC'GGCC_GC Cuts at: 3619 3619 Size: 6172 NspI r_CATG'y Cuts at: 1132 1229 2806 3530 5075 1132 Size: 97 1577 724 1545 2229 Fragments arranged by size: 2229 1577 1545 724 97 PasI CC'CwG_GG Cuts at: 1348 2332 2746 1348 Size: 984 414 4774 Fragments arranged by size: 4774 984 414 PciI A'CATG_T Cuts at: 5071 5071 Size: 6172 PflMI CCAn_nnn'nTGG Cuts at: 1108 3014 1108 Size: 1906 4266 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5754 5786 4241 Size: 32 1481 32 4627 Fragments arranged by size: 4627 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2467 3148 2467 Size: 681 5491 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6172 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PspOMI G'GGCC_C Cuts at: 2335 2685 2335 Size: 350 5822 PstI C_TGCA'G Cuts at: 830 3360 3423 3524 830 Size: 2530 63 101 3478 Fragments arranged by size: 3478 2530 101 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 3059 3059 Size: 6172 SacI G_AGCT'C Cuts at: 2300 2300 Size: 6172 SacII CC_GC'GG Cuts at: 2090 2090 Size: 6172 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SanDI GG'GwC_CC Cuts at: 3148 3148 Size: 6172 SapI GCTCTTCn'nnn_ Cuts at: 2288 2492 2288 Size: 204 5968 SbfI CC_TGCA'GG Cuts at: 3423 3524 3423 Size: 101 6071 ScaI AGT'ACT Cuts at: 4420 6132 4420 Size: 1712 4460 SfcI C'TryA_G Cuts at: 826 1045 3356 3419 3520 4655 5336 5527 826 Size: 219 2311 63 101 1135 681 191 1471 Fragments arranged by size: 2311 1471 1135 681 219 191 101 63 SfoI GGC'GCC Cuts at: 2093 2093 Size: 6172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6172 SmaI CCC'GGG Cuts at: 1872 2720 2777 3499 1872 Size: 848 57 722 4545 Fragments arranged by size: 4545 848 722 57 SmlI C'TyrA_G Cuts at: 820 1017 1943 4276 5177 5439 820 Size: 197 926 2333 901 262 1553 Fragments arranged by size: 2333 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 3530 1132 Size: 2398 3774 SspI AAT'ATT Cuts at: 5 52 4096 5 Size: 47 4044 2081 Fragments arranged by size: 4044 2081 47 StuI AGG'CCT Cuts at: 1517 3302 3425 1517 Size: 1785 123 4264 Fragments arranged by size: 4264 1785 123 StyI C'CwwG_G Cuts at: 513 1065 2325 2339 3283 3611 513 Size: 552 1260 14 944 328 3074 Fragments arranged by size: 3074 1260 944 552 328 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3311 4199 4216 4369 4554 4999 325 Size: 1255 1731 888 17 153 185 445 1498 Fragments arranged by size: 1731 1498 1255 888 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4418 6130 96 Size: 274 80 33 51 1443 2441 1712 138 Fragments arranged by size: 2441 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6132 6164 6132 Size: 32 6140 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6172 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6172 XmaI C'CCGG_G Cuts at: 1870 2718 2775 3497 1870 Size: 848 57 722 4545 Fragments arranged by size: 4545 848 722 57 XmnI GAAnn'nnTTC Cuts at: 2846 2978 4301 2846 Size: 132 1323 4717 Fragments arranged by size: 4717 1323 132 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3980 276 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI XcmI